Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 379,625 A→C V86G (GTT→GGT)  frmR ← DNA‑binding transcriptional repressor FrmR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096379,6250AC100.0% 678.0 / NA 173V86G (GTT→GGT) frmRDNA‑binding transcriptional repressor FrmR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (93/80);  total (93/80)

Only 100 of 173 total aligned reads displayed.

AGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTAT  >  U00096/379480‑379770
                                                                                                                                                 |                                                                                                                                                 
aGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCa                                                                                                                                                <  1:260477/149‑1 (MQ=255)
   aCTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGtt                                                                                                                                             <  1:260480/149‑1 (MQ=255)
   aCTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGtt                                                                                                                                             >  1:260563/1‑149 (MQ=255)
   aCTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGtt                                                                                                                                             >  1:260529/1‑149 (MQ=255)
    cTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTc                                                                                                                                            <  2:260481/149‑1 (MQ=255)
        aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATa                                                                                                                                        <  1:260485/149‑1 (MQ=255)
        aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATa                                                                                                                                        >  2:1234458/1‑149 (MQ=255)
        aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATa                                                                                                                                        >  1:260545/1‑149 (MQ=255)
        aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCACGCGGTTTACCGTGAGCAAATGCAACTGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATa                                                                                                                                        <  1:260484/149‑1 (MQ=255)
          cTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAgt                                                                                                                                      <  2:260486/149‑1 (MQ=255)
          cTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAgt                                                                                                                                      >  1:260552/1‑149 (MQ=255)
          cTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAgt                                                                                                                                      >  1:260550/1‑149 (MQ=255)
            tttttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAgtgt                                                                                                                                    <  2:260488/149‑1 (MQ=255)
             ttttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTgtc                                                                                                                                   <  1:260490/149‑1 (MQ=255)
               ttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTgtcgt                                                                                                                                 >  1:260608/1‑149 (MQ=255)
                 cGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCa                                                                                                                               <  2:260492/149‑1 (MQ=255)
                   gtggtgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAAc                                                                                                                             <  1:260493/149‑1 (MQ=255)
                   gtggtgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAAc                                                                                                                             >  2:260569/1‑149 (MQ=255)
                    tggtgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACg                                                                                                                            >  2:260536/1‑149 (MQ=255)
                    tggtgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACg                                                                                                                            >  2:260535/1‑149 (MQ=255)
                    tggtgCAACGTCAATTTCAACGATTTCCAGCGGGTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGCCCCAGTGCAATAGTGTCGTCAACg                                                                                                                            >  2:260547/1‑149 (MQ=255)
                      gtgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACgg                                                                                                                           <  1:260497/148‑1 (MQ=255)
                      gtgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGa                                                                                                                          <  1:260495/149‑1 (MQ=255)
                          aaCGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTgg                                                                                                                      <  2:260498/149‑1 (MQ=255)
                           aCGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGGCGTCAACGGATTGGc                                                                                                                     <  2:260500/149‑1 (MQ=255)
                               cAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGAc                                                                                                                 <  1:260501/149‑1 (MQ=255)
                                  tttCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTc                                                                                                              <  1:260504/149‑1 (MQ=255)
                                   ttCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcg                                                                                                             >  1:260542/1‑149 (MQ=255)
                                    tCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                                                            <  2:260505/149‑1 (MQ=255)
                                      aaCGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCgg                                                                                                          <  2:260507/149‑1 (MQ=255)
                                        cGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCt                                                                                                        >  1:260580/1‑149 (MQ=255)
                                         gATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTg                                                                                                       >  2:260560/1‑149 (MQ=255)
                                         gATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTg                                                                                                       >  1:260589/1‑149 (MQ=255)
                                          aTTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGt                                                                                                      >  2:260539/1‑149 (MQ=255)
                                             tCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGAtt                                                                                                                        <  1:260510/128‑1 (MQ=255)
                                             tCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGAtt                                                                                                                        >  2:260510/1‑128 (MQ=255)
                                               cAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAg                                                                                                 <  2:260511/149‑1 (MQ=255)
                                                    gTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCAtt                                                                                            >  2:260626/1‑149 (MQ=255)
                                                     tttACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCAttt                                                                                           <  2:260514/149‑1 (MQ=255)
                                                     ttcaCCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCAttt                                                                                           <  1:260513/146‑1 (MQ=255)
                                                      ttACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTAGTCAACGGATTGGCTGACTTCGAGGCTGTAGCAGTCATTTc                                                                                          >  2:260605/1‑149 (MQ=255)
                                                           gggAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCa                                                                                     >  2:260601/1‑149 (MQ=255)
                                                              aGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAAc                                                                                  <  2:260517/149‑1 (MQ=255)
                                                                cAAATGCAACGGCAGCACGTGATTTCCTCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCaaa                                                                                   <  1:260518/146‑1 (MQ=255)
                                                                 aaaTGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGtt                                                                               <  2:260520/149‑1 (MQ=255)
                                                                   aTGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTc                                                                             <  2:260521/149‑1 (MQ=255)
                                                                     gaaaCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGAACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTAGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTcac                                                                           >  2:260656/3‑147 (MQ=255)
                                                                     gCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTccc                                                                           <  2:260523/149‑1 (MQ=255)
                                                                      cAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCg                                                                          <  1:260525/149‑1 (MQ=255)
                                                                       aaCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTAAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCgg                                                                         >  1:260556/1‑149 (MQ=255)
                                                                        aCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGa                                                                        <  2:260526/149‑1 (MQ=255)
                                                                            cagcaCGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATg                                                                    <  2:260527/149‑1 (MQ=255)
                                                                             agcaCGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATgg                                                                   >  1:260622/1‑149 (MQ=255)
                                                                              gcaCGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGc                                                                  <  2:260529/149‑1 (MQ=255)
                                                                                 cGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCttt                                                               >  2:260566/1‑149 (MQ=255)
                                                                                 cGTGATTTCATCTCTCGCGCTTCCTCAATATGGTAATAGATACAGCTATTTAAGATAGGCACGACCCAGTACAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCAGTAGCAGGCATTTCGCTCAAACGTGTACCGGATATggaatt                                                               >  2:260624/1‑145 (MQ=255)
                                                                                     aTTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAg                                                           >  2:260607/1‑149 (MQ=255)
                                                                                         cATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACt                                                       <  2:260532/149‑1 (MQ=255)
                                                                                         cATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACt                                                       <  1:260531/149‑1 (MQ=255)
                                                                                          aTCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACtt                                                      >  1:260653/1‑149 (MQ=255)
                                                                                          aTCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACtt                                                      >  2:260594/1‑149 (MQ=255)
                                                                                             tctcGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGGCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCt                                                    <  1:260534/148‑1 (MQ=255)
                                                                                              ctcGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGTTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGc                                                  >  2:260582/1‑149 (MQ=255)
                                                                                              ctcGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTc                                                     <  1:260536/146‑1 (MQ=255)
                                                                                                 gCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGAGTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCAt                                               >  2:260590/1‑149 (MQ=255)
                                                                                                  ctctTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATc                                              <  1:260537/149‑1 (MQ=255)
                                                                                                     ttCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAg                                            >  1:260627/1‑148 (MQ=255)
                                                                                                      tCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGcc                                          >  1:260600/1‑149 (MQ=255)
                                                                                                      tCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGc                                           >  1:260573/1‑148 (MQ=255)
                                                                                                           aaTATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTa                                     >  1:260672/1‑149 (MQ=255)
                                                                                                           aaTATGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTa                                     >  1:260629/1‑149 (MQ=255)
                                                                                                            atatGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAg                                    >  1:260576/1‑149 (MQ=255)
                                                                                                              atGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGcc                                  >  2:260598/1‑149 (MQ=255)
                                                                                                               tGGTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGcc                                  >  1:260674/1‑148 (MQ=255)
                                                                                                                 gTAATAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCcgcg                               >  1:260596/1‑149 (MQ=255)
                                                                                                                   aaTAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCACCGGATTGGCTGACTTCGCGGCAGTAGCAGTCATTTCGGTCCAACGTTTACCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGcc                             >  2:260630/1‑149 (MQ=255)
                                                                                                                   aaTAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGcc                             <  1:260540/149‑1 (MQ=255)
                                                                                                                   aaTAGATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGcc                             >  2:260578/1‑149 (MQ=255)
                                                                                                                       gATTCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGcc                             >  1:260571/1‑145 (MQ=255)
                                                                                                                         ttCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAAc                       <  2:260542/149‑1 (MQ=255)
                                                                                                                          tCAGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACg                      >  2:260572/1‑149 (MQ=255)
                                                                                                                            aGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGc                    <  2:260544/149‑1 (MQ=255)
                                                                                                                            aGCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGc                    <  2:260545/149‑1 (MQ=255)
                                                                                                                             gCTATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTAGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGACATCAGCCCATTAGCCGCGCCCCGAACGGCa                   >  2:260616/1‑149 (MQ=255)
                                                                                                                             gCTATTTAAGATAGGCACGACCCAGGTCACTAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTGCGGGCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCa                   <  1:260547/149‑1 (MQ=255)
                                                                                                                               tATTTAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGc                 >  2:260587/1‑149 (MQ=255)
                                                                                                                                  ttAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGAt              <  2:260549/149‑1 (MQ=255)
                                                                                                                                   tAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATc             >  2:260662/1‑149 (MQ=255)
                                                                                                                                   tAAGATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATc             >  1:260617/1‑149 (MQ=255)
                                                                                                                                      gATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGt          >  1:260591/1‑149 (MQ=255)
                                                                                                                                      gATAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGt          <  2:260552/149‑1 (MQ=255)
                                                                                                                                       aTAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGtt         <  1:260553/149‑1 (MQ=255)
                                                                                                                                       aTAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGtt         >  2:260584/1‑149 (MQ=255)
                                                                                                                                       aTAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGACAAACGTTTCCCGGATATGGCTTTCAAGCACTACTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGtt         >  2:260644/1‑149 (MQ=255)
                                                                                                                                        tAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTg        <  2:260554/149‑1 (MQ=255)
                                                                                                                                        tAGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTACGGTCAAACGTTTCCCGGATATGGCGTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAATGGCAGCGATCTGTTg        >  1:260633/1‑149 (MQ=255)
                                                                                                                                         aGGCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTgg       >  1:2137441/1‑149 (MQ=255)
                                                                                                                                          ggCACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGa      <  2:260556/149‑1 (MQ=255)
                                                                                                                                            cACGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGt    <  2:260558/149‑1 (MQ=255)
                                                                                                                                             aCGACCCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTa   >  2:260618/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTAT  >  U00096/379480‑379770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑