Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 1,152,159 T→G F74C (TTC→TGC)  fabF → 3‑oxoacyl‑[acyl carrier protein] synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,152,1590TG99.4% 679.3 / NA 175F74C (TTC→TGC) fabF3‑oxoacyl‑[acyl carrier protein] synthase 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (90/84);  total (90/85)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

GAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTTCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACATCTCTG  >  U00096/1152013‑1152304
                                                                                                                                                  |                                                                                                                                                 
gAAAGCTCTGCTTGCCGGTCAGAGGGGCATCAGCCTAAGCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCtgca                                                                                                                                                 <  2:804118/149‑1 (MQ=255)
 aaaGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCtgcat                                                                                                                                                <  1:804120/149‑1 (MQ=255)
 aaaGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCtgca                                                                                                                                                 >  1:804189/1‑148 (MQ=255)
   aGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCtgcatt                                                                                                                                               >  1:804123/1‑148 (MQ=255)
       ctGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAata                                                                                                                                          <  1:804126/149‑1 (MQ=255)
        tGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAatat                                                                                                                                         >  1:804173/1‑149 (MQ=255)
         gCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATg                                                                                                                                        <  2:804127/149‑1 (MQ=255)
         gCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATg                                                                                                                                        <  1:804128/149‑1 (MQ=255)
          cTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATgg                                                                                                                                       <  2:804131/149‑1 (MQ=255)
           ttGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGa                                                                                                                                      >  1:804260/1‑149 (MQ=255)
           ttGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGa                                                                                                                                      <  2:1855340/149‑1 (MQ=255)
                ggTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGTGCAAGATGGATGCCTGCATTCAATATGGAATTGt                                                                                                                                 <  1:804133/149‑1 (MQ=255)
                ggTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGAGTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCCAGATGGATGCCTGCATTCAATATGGAATTGt                                                                                                                                 >  1:804226/1‑149 (MQ=255)
                 gTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGt                                                                                                                                 <  1:804136/148‑1 (MQ=255)
                 gTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTc                                                                                                                                <  2:804137/149‑1 (MQ=255)
                 gTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTc                                                                                                                                <  1:2520951/149‑1 (MQ=255)
                     gattGGCAGCAGCCTCAGCGACAATTTCGATACAAGCGCCTATTCAACGAAATTTGCTTGCTTAGTAAAGGATTTTAACTGTGATGACATTATCTCGCGCAAAGAACCGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTg                                                                                                                            <  2:804139/146‑1 (MQ=255)
                     gagTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCt                                                                                                                             >  1:804272/1‑148 (MQ=255)
                            aTCAGCCTAATCGACCAGTGCGATACTAGCGCCTCTGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGAGGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCa                                                                                                                     <  1:804142/149‑1 (MQ=255)
                               aGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGATATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAg                                                                                                                    >  1:2891638/1‑147 (MQ=255)
                               aGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGc                                                                                                                  <  2:804143/149‑1 (MQ=255)
                                gCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGcc                                                                                                                 >  2:804214/1‑149 (MQ=255)
                                gCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGcc                                                                                                                 <  2:804144/149‑1 (MQ=255)
                                 ccTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCa                                                                                                                >  2:804256/1‑149 (MQ=255)
                                   tAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATg                                                                                                              <  1:804147/149‑1 (MQ=255)
                                    aaTCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGc                                                                                                             <  2:804149/149‑1 (MQ=255)
                                    aaTCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGc                                                                                                             >  1:804236/1‑149 (MQ=255)
                                      tCGCCCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTATCTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCTTTCATTATGGAATTGTCGCTGGCGTTCAGGCCATGCAg                                                                                                           >  2:804267/1‑149 (MQ=255)
                                      tCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAg                                                                                                           >  1:804201/1‑149 (MQ=255)
                                      tCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAg                                                                                                           >  2:804223/1‑149 (MQ=255)
                                       cGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAgg                                                                                                          <  1:804152/149‑1 (MQ=255)
                                         aCCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGAt                                                                                                        >  2:804153/1‑149 (MQ=255)
                                            aTTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCt                                                                                                     >  2:804199/1‑149 (MQ=255)
                                              ttCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTgg                                                                                                   <  2:804155/149‑1 (MQ=255)
                                               tCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTgg                                                                                                   >  1:804299/1‑148 (MQ=255)
                                                  aTACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCtt                                                                                               >  1:804232/1‑149 (MQ=255)
                                                    aCTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGATGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTTCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGa                                                                                             <  2:804158/149‑1 (MQ=255)
                                                     cTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGaa                                                                                            <  1:804159/149‑1 (MQ=255)
                                                      tAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGaaa                                                                                           >  1:804233/1‑149 (MQ=255)
                                                          gcCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAAc                                                                                       <  2:804163/149‑1 (MQ=255)
                                                          cccTATGCAACGAAATTGGCTGGCTTAGTAAAGGATTTTAACTGTGATGACATTACCTCGCGCAAAGAACAGCGCAAGATGGATTCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAAc                                                                                       <  2:804161/148‑1 (MQ=255)
                                                            cTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACgg                                                                                     >  2:804317/1‑149 (MQ=255)
                                                            cTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACgg                                                                                     <  1:1449739/149‑1 (MQ=255)
                                                             tATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGa                                                                                    <  1:804166/149‑1 (MQ=255)
                                                              aTGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGaa                                                                                   <  2:804167/149‑1 (MQ=255)
                                                                gCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGATCAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAaga                                                                                 <  1:804170/149‑1 (MQ=255)
                                                                gCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGa                                                                                    >  2:804246/1‑146 (MQ=255)
                                                                  aaCGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAag                                                                                  >  1:804213/1‑146 (MQ=255)
                                                                  aaCGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAgaga                                                                               <  2:804172/149‑1 (MQ=255)
                                                                   aCGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGaa                                                                              >  2:804217/1‑149 (MQ=255)
                                                                    cGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAAc                                                                             <  2:804175/149‑1 (MQ=255)
                                                                      aaaTTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGc                                                                           >  2:804251/1‑149 (MQ=255)
                                                                      aaaTTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGc                                                                           >  1:804284/1‑149 (MQ=255)
                                                                           tGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAAccc                                                                      <  2:804178/149‑1 (MQ=255)
                                                                            gCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCa                                                                          >  1:804247/1‑144 (MQ=255)
                                                                             cTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGc                                                                    >  2:804294/1‑149 (MQ=255)
                                                                                gCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCAtt                                                                 <  2:1827393/149‑1 (MQ=255)
                                                                                gCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCAt                                                                  >  1:804295/1‑148 (MQ=255)
                                                                                  ttAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTgg                                                               <  2:804182/149‑1 (MQ=255)
                                                                                  ttAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTgg                                                               >  2:804238/1‑149 (MQ=255)
                                                                                  tgaGTAAAGGATTTTTACTTTGAGGACATTATCTCGAGCAAAGATCAGCGCAAGATGGATGCCTGCATTCAATATGGCATTGTCGCTGGCGTGCAGGCCAGGCAGGATTCTGGTCTTGAAATAACGGAAGAGAACGCAACCCGCATTgg                                                               <  1:804180/147‑1 (MQ=255)
                                                                                   tAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGt                                                              <  1:804183/149‑1 (MQ=255)
                                                                                   tAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGt                                                              >  1:804274/1‑149 (MQ=255)
                                                                                     gTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGc                                                            <  1:804185/149‑1 (MQ=255)
                                                                                      tAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGcc                                                           >  1:804291/1‑149 (MQ=255)
                                                                                      tAAAGGATTTTAACAGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATCGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGcc                                                           >  2:804230/1‑149 (MQ=255)
                                                                                          ggATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCaa                                                       >  2:804266/1‑149 (MQ=255)
                                                                                            aTTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAAtt                                                     <  2:804187/149‑1 (MQ=255)
                                                                                             ttttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTg                                                    >  1:804287/1‑149 (MQ=255)
                                                                                             ttttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTg                                                    <  1:804188/149‑1 (MQ=255)
                                                                                              tttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTgg                                                   >  1:804302/1‑149 (MQ=255)
                                                                                               ttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGc                                                  >  1:1743078/1‑149 (MQ=255)
                                                                                               ttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGc                                                  <  2:804189/149‑1 (MQ=255)
                                                                                               ttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGc                                                  >  1:804280/1‑149 (MQ=255)
                                                                                                 aaCTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTc                                                >  1:804242/1‑149 (MQ=255)
                                                                                                         ggACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTg                                        >  1:804327/1‑149 (MQ=255)
                                                                                                           aCATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTggc                                      <  2:804194/149‑1 (MQ=255)
                                                                                                           aCATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTggc                                      >  1:804332/1‑149 (MQ=255)
                                                                                                           aCATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTggc                                      <  1:804195/149‑1 (MQ=255)
                                                                                                             aTTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTggcgg                                    >  2:804259/1‑149 (MQ=255)
                                                                                                              ttATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCATGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTggcggc                                   >  2:804248/1‑149 (MQ=255)
                                                                                                               tATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGcc                                  >  2:804262/1‑149 (MQ=255)
                                                                                                                 tctcGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAAc                                                                                       <  1:804197/94‑1 (MQ=255)
                                                                                                                    cgcgCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGa                             <  1:804199/149‑1 (MQ=255)
                                                                                                                         aaaGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGAt                        <  2:804201/149‑1 (MQ=255)
                                                                                                                          aaGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATc                       <  2:804203/149‑1 (MQ=255)
                                                                                                                             aaCAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaa                    <  1:804204/149‑1 (MQ=255)
                                                                                                                              aCAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaag                   >  1:804265/1‑149 (MQ=255)
                                                                                                                              aCAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCACTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaag                   >  2:804254/1‑149 (MQ=255)
                                                                                                                                aGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaagaa                 >  2:804278/1‑149 (MQ=255)
                                                                                                                                 gcgcAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGaaa                <  1:804205/149‑1 (MQ=255)
                                                                                                                                  cgcAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGaaaa               <  1:804208/149‑1 (MQ=255)
                                                                                                                                   gcAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGgaagc              >  2:848214/1‑147 (MQ=255)
                                                                                                                                        atggatgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAAc              >  1:804271/1‑144 (MQ=255)
                                                                                                                                        atggatgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACcacac         <  2:804210/149‑1 (MQ=255)
                                                                                                                                            atgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGaaa                >  1:804309/1‑138 (MQ=255)
                                                                                                                                            atgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACAt       <  1:804211/147‑1 (MQ=255)
                                                                                                                                             tgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACAtctc    <  2:804213/149‑1 (MQ=255)
                                                                                                                                              gCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACATctct   <  1:804215/149‑1 (MQ=255)
                                                                                                                                               ccTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGCACGCACCCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACATCTCtg  >  2:804326/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTTCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACATCTCTG  >  U00096/1152013‑1152304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑