Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306472,8680TG24.2% 562.8 / 19.7 211D435A (GAC→GCC) cusSsensory histidine kinase in two‑component regulatory system with CusR, senses copper ions
Reads supporting (aligned to +/- strand):  ref base T (34/126);  new base G (51/0);  total (85/126)
Fisher's exact test for biased strand distribution p-value = 2.01e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 282 total aligned reads displayed.

TGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTC  >  AP012306/472719‑473017
                                                                                                                                                     |                                                                                                                                                      
   tGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCaa                                                                                                                                                     <  2:296242/150‑1 (MQ=255)
     aaGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAAcg                                                                                                                                                   <  1:612514/150‑1 (MQ=255)
     aaGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAAcg                                                                                                                                                   >  1:4041100/1‑150 (MQ=255)
        gATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGa                                                                                                                                                >  2:1668286/1‑150 (MQ=255)
          ttAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATa                                                                                                                                              <  1:2515434/150‑1 (MQ=255)
          ttAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATa                                                                                                                                              >  1:836849/1‑150 (MQ=255)
           tAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGACCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGAGGGGCAACGCGagga                                                                                                                                             >  2:1000385/1‑147 (MQ=255)
              gCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGaaa                                                                                                                                          <  2:1876768/150‑1 (MQ=255)
              gCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGaaa                                                                                                                                          <  1:1151440/150‑1 (MQ=255)
               cGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAAc                                                                                                                                         >  1:2106330/1‑150 (MQ=255)
                gggTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCACAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACg                                                                                                                                        >  1:2538954/1‑150 (MQ=255)
                  gTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGt                                                                                                                                      >  1:2480375/1‑150 (MQ=255)
                    aaTGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCa                                                                                                                                    <  1:3503211/150‑1 (MQ=255)
                     aTGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCaa                                                                                                                                   >  1:2405282/1‑150 (MQ=255)
                      tgtgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCaaa                                                                                                                                  <  1:2840324/150‑1 (MQ=255)
                      tgtgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCaaa                                                                                                                                  <  2:3731321/150‑1 (MQ=255)
                      tgtgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAAAGGTCaaa                                                                                                                                  >  1:224936/1‑150 (MQ=255)
                       gtgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGTGGGGGGAACGCGTTAAAAGCAGGCAAAc                                                                                                                                 >  2:1576701/1‑150 (MQ=255)
                       gtgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAAc                                                                                                                                 <  2:1484405/150‑1 (MQ=255)
                       gtgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAAAGGTCAAAc                                                                                                                                 >  2:1745310/1‑150 (MQ=255)
                        tgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACCCGATAGAAACAGTcacaca                                                                                                                                >  1:3219755/1‑150 (MQ=255)
                           tAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATc                                                                                                                             <  1:1538268/150‑1 (MQ=255)
                             aCAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCCGTAGAAACCGTCcaacaaaagc                                                                                                                           >  2:1394972/1‑146 (MQ=255)
                               aaaCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAAAGGGCCAACAAACCCgg                                                                                                                         >  2:2226677/1‑150 (MQ=255)
                                   cTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGTCAAACAATCCCGG‑TAAg                                                                                                                    >  1:673829/1‑150 (MQ=255)
                                   cTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAAAGCGGTAGAAACCGGCAAACAATAGCGG‑ttat                                                                                                                    >  1:2919647/1‑147 (MQ=255)
                                    ttGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGt                                                                                                                   <  1:2157631/150‑1 (MQ=255)
                                      gTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGGTAGAAAAGGGCAAAAAAACGCGG‑GAAGGGc                                                                                                                 >  2:2685417/1‑150 (MQ=255)
                                       tCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCt                                                                                                                >  1:1599192/1‑150 (MQ=255)
                                         ccccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCg                                                                                                              <  2:515889/150‑1 (MQ=255)
                                          cccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCCATAAAAACAGTCAAAAAAAAGCGG‑CAAGGGCTCgg                                                                                                             >  2:3270969/1‑150 (MQ=255)
                                             gcgcATCCGACGTTACCGAAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATTCCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGgcg                                                                                                          <  1:1014962/150‑1 (MQ=255)
                                               gcATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCa                                                                                                        <  1:1892429/150‑1 (MQ=255)
                                               gcATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCCACCCGATAGAAACGGGCAAACAATCGCGG‑GAAGTGGCCCGGCGCa                                                                                                        >  1:3192810/1‑150 (MQ=255)
                                               gcATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACACGAAAGAAACGGTCAAACAAACGCGG‑TAAGTGGCCGGGCGCa                                                                                                        >  2:261265/1‑150 (MQ=255)
                                                cATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCaa                                                                                                       >  1:1991577/1‑150 (MQ=255)
                                                 aTCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAAt                                                                                                      <  1:3596981/150‑1 (MQ=255)
                                                  tCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGGCAAACAACCGCGG‑GAAGTGCACCGGCGCAATg                                                                                                     >  1:3451868/1‑150 (MQ=255)
                                                   ccGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATgg                                                                                                    <  1:232014/150‑1 (MQ=255)
                                                    cGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCCACGCGATAGAAACGGTCCAACAATCGCGG‑GAAGTGCTCGGGCGCAATggg                                                                                                   >  2:3200469/1‑150 (MQ=255)
                                                     gACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAAAAACCGTCCAACAATCGCCG‑GAAGTGCCCCGGGCCAATGGGc                                                                                                  >  1:3931642/1‑150 (MQ=255)
                                                      aCGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGCAGGGTCAACCCGATAAAAACGGGCAAACAATCGCGG‑GTAGTGACCGGGCGCAACggggg                                                                                                 >  2:2337558/1‑148 (MQ=255)
                                                        gTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTa                                                                                               >  1:895229/1‑150 (MQ=255)
                                                            ccGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑GAAGTGCTCGGGCGCAATGGGCGGACCgg                                                                                           >  2:1267047/1‑150 (MQ=255)
                                                                aaCCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGtt                                                                                       <  1:1356188/150‑1 (MQ=255)
                                                                aaCCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGGTAGAAAACGGCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCgggggt                                                                                       >  2:4081338/1‑148 (MQ=255)
                                                                 aCCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGttt                                                                                      <  1:4011032/150‑1 (MQ=255)
                                                                 aCCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑GAAGTGCTCGGGCGCAATGGGCGGACCGGGGgtt                                                                                      >  1:2198540/1‑150 (MQ=255)
                                                                    gTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATTGGCGTACCGGGGGTTTcg                                                                                   >  1:365672/1‑150 (MQ=255)
                                                                      gCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAAAGGTCAAACAAACGCGG‑TAAGTGCTCGGGGGCAATGGGCGTACCGGGGGTTTtgac                                                                                 >  2:1987435/1‑150 (MQ=255)
                                                                       ccTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGGGCAATGGGCGTACCGGGGGTTTcgacg                                                                                >  2:3249129/1‑150 (MQ=255)
                                                                        cTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGTCAACGCGATAGAAACGGTCAAACAAACGCGG‑TAAGGGCTCGGGCGGAAAAGGGGGAACGGGGGTTTtgacga                                                                               >  2:1920509/1‑150 (MQ=255)
                                                                        cTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGGGCTCGGGCGCAATGGGCGTACCCGGGGTTTtgacga                                                                               >  1:1646896/1‑150 (MQ=255)
                                                                           tATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATaa                                                                            <  1:4147626/150‑1 (MQ=255)
                                                                           tATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATaa                                                                            <  1:2906118/150‑1 (MQ=255)
                                                                             tGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGGTAGAAACGGGCAAACAATCGCGG‑TAAGTGCTCGGGCGCCATGGGCGTTCCCGGGGTTTTGACGATaaat                                                                          >  2:2504087/1‑148 (MQ=255)
                                                                              gcgcGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACtt                                                                         <  2:1116396/150‑1 (MQ=255)
                                                                                      acaaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAgg                                                                 <  1:1294086/150‑1 (MQ=255)
                                                                                          tCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGAt                                                             <  2:1674078/150‑1 (MQ=255)
                                                                                           cGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATc                                                            <  1:1487075/150‑1 (MQ=255)
                                                                                           cGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATc                                                            <  1:2968490/150‑1 (MQ=255)
                                                                                            gATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGGAAAGGGGGAGAAAAGGGGAAAAAAAAGGGG‑GAAGGGGGGGGGGGGGATGGGGGGAGCGGGGGTTTTGACGAGAAATTGGACCAgggggagg                                                           >  2:425285/1‑144 (MQ=255)
                                                                                             aTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGCGGGGTCAACGCGATAAAAACGGGTAAACAAACGCGGCAAAGTGCCGGGGCGC‑ATGGGCGTTCCCGGGATGTTGAGGCGAACCTTCACCAGGAGGTCGa                                                          >  2:2768169/1‑150 (MQ=255)
                                                                                             aTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑GAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGa                                                          >  1:2805645/1‑150 (MQ=255)
                                                                                                   cTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCt                                                    <  2:902566/150‑1 (MQ=255)
                                                                                                    tATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGGTAGAAAAGGTCAAACAATTGCGG‑TAAGTGCTCGGGCGCAATTGGCGTAACGGGGGTTTCGACGATAACTTGCACCAGGTGGTCGACCGTCTg                                                   >  2:2284158/1‑150 (MQ=255)
                                                                                                    tATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGTCAAAAAATCCCGG‑TAAATGCTCGGGCGCAAAGGGGGGACCGGGGGTTTTGACGGTAACTTTCACCAAGGGATCGACCGTCTg                                                   >  2:3542071/1‑150 (MQ=255)
                                                                                                       cGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACa                                                <  1:3567590/150‑1 (MQ=255)
                                                                                                        gCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAg                                               >  1:1077177/1‑150 (MQ=255)
                                                                                                           aGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCCATGGGCGTACCCGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAgcgc                                            >  1:3764410/1‑150 (MQ=255)
                                                                                                           aGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAgcgc                                            >  1:3041360/1‑150 (MQ=255)
                                                                                                               cAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑GAAGTGCTCGGGCGCAATGGGGGTACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCACTa                                        >  2:477982/1‑150 (MQ=255)
                                                                                                               cAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTa                                        <  2:657663/150‑1 (MQ=255)
                                                                                                                aaTACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCCACGCGATAGAAACGGGCAAACAATCGCGG‑GAAGTGGTCGGGCGCAATGGGGGTACCGGGGGTTTTGACGATAAATTTCACCAGGGGATCGACCCTCTGACAGCGCCCTAc                                       >  2:3600866/1‑150 (MQ=255)
                                                                                                                aaTACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTAc                                       <  1:1067275/150‑1 (MQ=255)
                                                                                                                aaTACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGGCAAACAATCGCGG‑GAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTAc                                       >  1:261829/1‑150 (MQ=255)
                                                                                                                aaTACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTAc                                       >  2:580650/1‑150 (MQ=255)
                                                                                                                  tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACaa                                     <  2:145581/150‑1 (MQ=255)
                                                                                                                   aCCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTTGACGATAACTTGCACCAGGTGGTCGACCGTCTGACAGCGCACTACAAt                                    >  2:3447037/1‑150 (MQ=255)
                                                                                                                    ccGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGGCAAACAATCACGG‑TAAAAGGCCGGGGGCACTGGGCGTACCGGGGGTTTCGACGATAAATTGCCCCAGGGGATCGACCGTCTTGCAGCGCACTCCAAtt                                   >  2:1962548/1‑150 (MQ=255)
                                                                                                                       cTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtc                                >  1:3881352/1‑150 (MQ=255)
                                                                                                                          ccTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtctct                             <  1:3793982/150‑1 (MQ=255)
                                                                                                                           cTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtctctc                            <  2:2854395/150‑1 (MQ=255)
                                                                                                                           cTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtctctc                            <  1:4108274/150‑1 (MQ=255)
                                                                                                                            ttCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAAAGGTCAAACAATCGCGG‑GAAGTGCTCGGGCGCAATGGGCGTAACGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCACTACAATTGTCTCTcc                           >  2:3860934/1‑150 (MQ=255)
                                                                                                                             tCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCg                          <  2:3796846/150‑1 (MQ=255)
                                                                                                                                 cTTTTCGCTGGCGGGAGGGGGCAACGCGGGAAAAACGGTGAAAAAATCGCCG‑GAAGGGGCCCGGTGCTAGGGGCCTGCCGGGGTTTTTGGCGATAACTTGCCCCGGGTGAGCGCCCGTCCGAAAGGCCACTACAATTTTACCTCCgcatg                      >  2:21573/1‑146 (MQ=255)
                                                                                                                                  ttttCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTgg                     <  2:1045456/150‑1 (MQ=255)
                                                                                                                                   tttCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGc                    >  1:3349219/1‑150 (MQ=255)
                                                                                                                                   tttCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGc                    <  2:2378224/150‑1 (MQ=255)
                                                                                                                                      cGCTGGCGGGAGGGGGCAACGCGATAGGAACGGGCAAACAATCCCGG‑TAAGTGCTCGGGCGCGATGGGCGGGCCGGGGTTTTCGACGATAACTTGCACCAGGTGATAGACCGTCTGACAGCGCACTCCAATTTTCTCTCCAGTTGGCGta                 >  2:1150566/1‑150 (MQ=255)
                                                                                                                                       gCTGGCGGGAGGTGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGtat                <  2:2077552/150‑1 (MQ=255)
                                                                                                                                       gCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGtat                <  2:3116830/150‑1 (MQ=255)
                                                                                                                                        cTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑GAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGtata               >  2:1245112/1‑150 (MQ=255)
                                                                                                                                          ggCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAAc             <  1:1207246/150‑1 (MQ=255)
                                                                                                                                             gggAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCa          >  1:1799228/1‑150 (MQ=255)
                                                                                                                                              ggAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAg         <  1:2076201/150‑1 (MQ=255)
                                                                                                                                                aGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAggg       >  2:3032243/1‑150 (MQ=255)
                                                                                                                                                  gggTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCg     <  2:3318819/150‑1 (MQ=255)
                                                                                                                                                    gTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGtt   <  2:756597/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGG‑TAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTC  >  AP012306/472719‑473017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑