Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT23.8% 296.0 / 39.3 101intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (15/62);  new base T (24/0);  total (39/62)
Fisher's exact test for biased strand distribution p-value = 2.40e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 175 total aligned reads displayed.

GCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAAC  >  AP012306/934906‑935202
                                                                                                                                                     |                                                                                                                                                        
 cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑Gtttttt                                                                                                                                                        >  2:516011/1‑150 (MQ=255)
   gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑ttttttttt                                                                                                                                                      >  1:1494540/1‑149 (MQ=255)
    tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑tttttttttg                                                                                                                                                     >  1:1982953/1‑148 (MQ=255)
    tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑tttttttttg                                                                                                                                                     >  1:4316050/1‑148 (MQ=255)
    tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTgg                                                                                                                                                     <  1:111722/150‑1 (MQ=255)
     aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑gtttgttttgg                                                                                                                                                    >  2:4354103/1‑147 (MQ=255)
     aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGc                                                                                                                                                    <  2:3747915/150‑1 (MQ=255)
      ttGGGATTGTAGTGTTTAGTTTCCTCGGTAAAGCGCCGTCACCGGCGATGCAAAAACGCTTTGCCGAGGCTGTTGCGCACTATCATTCGGCTCCGCGGTCACGGTTGCAGGAAAGCTAAGGATCTTCGCGTGTGGCGG‑ATTTGTTAggat                                                                                                                                                   >  2:1350817/1‑148 (MQ=255)
       tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑tttttttttggtt                                                                                                                                                  >  2:2521261/1‑145 (MQ=255)
       tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCtt                                                                                                                                                  >  2:1636354/1‑150 (MQ=255)
       tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCtt                                                                                                                                                  >  1:2417204/1‑150 (MQ=255)
       tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑Gttttttttgttt                                                                                                                                                  >  2:863538/1‑145 (MQ=255)
        gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCttt                                                                                                                                                 >  2:2920302/1‑149 (MQ=255)
          gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCtttcg                                                                                                                                               >  1:3676927/1‑147 (MQ=255)
          gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTTGCTTctg                                                                                                                                               >  1:1219224/1‑148 (MQ=255)
           aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTcacc                                                                                                                                              >  1:1413964/1‑148 (MQ=255)
           aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTTGCCTccgg                                                                                                                                              >  1:482325/1‑147 (MQ=255)
           aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTGTTTTGCTTctgg                                                                                                                                              >  2:2748255/1‑147 (MQ=255)
               tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTTGCTTCAGCAG‑Cg                                                                                                                                         >  1:513343/1‑150 (MQ=255)
               tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑Cg                                                                                                                                         <  1:3732461/150‑1 (MQ=255)
                aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTTGCTTTTGCAA‑Cgg                                                                                                                                        >  1:2562012/1‑150 (MQ=255)
                 gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTTAGCCA‑Cggg                                                                                                                                       >  2:2661927/1‑150 (MQ=255)
                    tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTTCGCAG‑CGGGGTg                                                                                                                                    >  2:3490596/1‑150 (MQ=255)
                    tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTCCGCCGCCGGGtt                                                                                                                                     >  1:1561500/1‑150 (MQ=255)
                    tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTGGCTTGTGCAA‑Cgggcct                                                                                                                                    >  1:609990/1‑147 (MQ=255)
                      tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGCTT‑AGCAG‑CGGGGTggg                                                                                                                                  >  2:2315184/1‑148 (MQ=255)
                      tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTgcg                                                                                                                                  <  2:2984902/150‑1 (MQ=255)
                      tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTTGGTTCCGCCC‑CGGGTTtcg                                                                                                                                  >  1:3771695/1‑150 (MQ=255)
                          ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTGGCTTTAGCAG‑CGGGGTGCG‑CTcc                                                                                                                             >  1:1141816/1‑150 (MQ=255)
                            gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTTACCCG‑CGGGTTGCG‑CCCCCt                                                                                                                           >  1:956329/1‑150 (MQ=255)
                            gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTCTGCAG‑CGGGGTTCG‑CTcccc                                                                                                                           >  1:2998693/1‑149 (MQ=255)
                            gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTTGCTTCCGCAG‑CGGGGTGCG‑CTcccc                                                                                                                           >  1:2198780/1‑149 (MQ=255)
                             cTGGGTAAAGCGCCGTCAGCGGGGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCtt                                                                                                                          <  2:1826726/150‑1 (MQ=255)
                             cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTCCGCAG‑CGGGGTTCG‑CTcccct                                                                                                                          >  2:3270003/1‑148 (MQ=255)
                             cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTCCGCAG‑CGGGGTGCG‑CTcccct                                                                                                                          >  1:3420344/1‑148 (MQ=255)
                             cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCtt                                                                                                                          <  2:2955506/150‑1 (MQ=255)
                             cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCtt                                                                                                                          <  2:1987188/150‑1 (MQ=255)
                              tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTa                                                                                                                         <  2:1537620/150‑1 (MQ=255)
                              tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTTGGTTCTGCAG‑CGGGGTGGG‑CTcccctt                                                                                                                         >  2:3865325/1‑147 (MQ=255)
                                ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAAt                                                                                                                       <  2:1498322/150‑1 (MQ=255)
                                ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTTGCTTCAGCAG‑CGGGGTGCG‑CTccccttat                                                                                                                       >  2:2421087/1‑145 (MQ=255)
                                ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTGTTTTGTTTCTCCAG‑CGGGTTGCG‑CTccccttat                                                                                                                       >  2:121134/1‑145 (MQ=255)
                                 gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAAtg                                                                                                                      <  1:3826587/150‑1 (MQ=255)
                                  tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTGGGTTCCGCCG‑CGGGGTGGG‑GTCCCCTTAtgt                                                                                                                     >  2:2716780/1‑150 (MQ=255)
                                  tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTGGGTTCAGCAG‑CGGGGTGCG‑CGCCCTTAAttt                                                                                                                     >  1:279569/1‑148 (MQ=255)
                                     aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑ATTTTTTTTGCTTCAGCCG‑CGGGGTTCG‑CTCCCCTAAtgtaac                                                                                                                  >  1:1319268/1‑147 (MQ=255)
                                        gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCg                                                                                                               <  2:3680558/150‑1 (MQ=255)
                                        gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTGGCTTCAGCAG‑CGGGGTGGG‑CGCCCCTTATTTGGCTCg                                                                                                               >  2:449141/1‑150 (MQ=255)
                                         ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTCAGCAG‑CGGGGTGCG‑CTCCCCTTATTTGCCTCGc                                                                                                              >  1:1978901/1‑150 (MQ=255)
                                            tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTCCGCAG‑CGGGGTGCG‑CTCCCCTTATTTGCCTCGccct                                                                                                           >  1:594049/1‑148 (MQ=255)
                                              aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTGGCTTCTGCAG‑CGGGGTGGG‑CTCCCCTCATTTGCCTCGcccttt                                                                                                         >  2:1188345/1‑146 (MQ=255)
                                              aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCatat                                                                                                         <  1:2146857/150‑1 (MQ=255)
                                               gcggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCAtata                                                                                                        <  1:950212/150‑1 (MQ=255)
                                                cggcgATGCAAAAACGTTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATaa                                                                                                       <  2:1376040/150‑1 (MQ=255)
                                                cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTTGCTTCAGCAG‑CGGGGTGCG‑CCCCCCTTATGTGCCTCGCCCTATaa                                                                                                       >  2:3412471/1‑150 (MQ=255)
                                                cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATaa                                                                                                       <  1:1679336/150‑1 (MQ=255)
                                                 ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTTGCTTCAGCAG‑CGGGGTGCG‑GTTCCTTTATGTGCCTCGCCCTATtaa                                                                                                      >  2:815956/1‑150 (MQ=255)
                                                 ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATaaa                                                                                                      <  1:3916593/150‑1 (MQ=255)
                                                 ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATaaa                                                                                                      <  2:3331108/150‑1 (MQ=255)
                                                   cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTTGCTTCCGCAG‑CGGGGTGGG‑CCCCCCTTATTTGTCTCGCCCTATaaaat                                                                                                    >  1:1027471/1‑148 (MQ=255)
                                                    gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTGTTTTGCGTTTGCAG‑CGGGGTGCG‑CCCCCCTAATGTGGCTCGCCGTATAAAATg                                                                                                   >  2:65812/1‑150 (MQ=255)
                                                     aTGCAAAACCGCTTTGCCGAGGCGGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑a                                                                                                 <  2:931364/150‑1 (MQ=255)
                                                      tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑aa                                                                                                <  1:1609592/150‑1 (MQ=255)
                                                      tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑CTTTTTTTGGCTTCAGCAG‑CGGGGTGCG‑CCCCCCTTATGTGCCTCGCCCTATAAATTG‑aa                                                                                                >  1:3990246/1‑150 (MQ=255)
                                                       gCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTCAGCAG‑CGGGGTGCG‑CTCCCCTTATTTGCCTCGCCCTATAAATTT‑AAt                                                                                               >  1:97860/1‑150 (MQ=255)
                                                       gCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑GTTTTTTTTGCTTCAGCAG‑CGGGGTGCG‑CTCCCCTAATTTGCCTCGCCCTATAAATTT‑AAt                                                                                               >  1:811178/1‑150 (MQ=255)
                                                        cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATg                                                                                              <  1:4224074/150‑1 (MQ=255)
                                                          aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGt                                                                                            <  1:1323355/150‑1 (MQ=255)
                                                           aaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTCAGCAG‑CGGGGTGGG‑CGCCCCTTAAGTGGCTCGCCCTTTAAATTT‑AATGGTg                                                                                           >  2:3586218/1‑150 (MQ=255)
                                                               gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCCTTCGCAG‑CGGGTTGCG‑GGCCCTTTATTTGGCTCCCCCATTAAAATT‑AATggcggtgg                                                                                       >  2:2828445/1‑144 (MQ=255)
                                                               gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAgg                                                                                       <  1:4315223/150‑1 (MQ=255)
                                                                 tttGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGa                                                                                     <  1:2205467/150‑1 (MQ=255)
                                                                    gCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTCCGCCG‑CGGGGTGCG‑CGCCCCTAATGTGGCTCGCCATATAAATTT‑TATGGGGGAGGGGgcg                                                                                  >  2:592791/1‑150 (MQ=255)
                                                                     ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTCCGCAG‑CGGGGTGGG‑CCCCCCTTATTTGGCCCGCCCTATAAAATT‑AAAAGTGCAgggggggg                                                                                 >  2:3962430/1‑145 (MQ=255)
                                                                     ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAgcgc                                                                                 <  2:3403471/150‑1 (MQ=255)
                                                                           ccGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGGTTTGGTTTTGCAG‑CGGGGTGCG‑CGCCCCTTATGTGCCTCGCCCTATTAAATG‑AATGGGGGAGGGGGGGCGCgggg                                                                           >  2:695896/1‑150 (MQ=255)
                                                                            cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTTAGCAG‑CGGGGTGGG‑CCCCCCTTATTTGGCTCCCCCTATAAATTT‑AATGGTGGAGGGGGGGCGCCgggg                                                                          >  2:2105082/1‑150 (MQ=255)
                                                                            cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTTTGGTCTGCCG‑CGGGGTTTG‑CTCCCTTAAATTGGCTTCCCCCGCTAATTG‑GATAGTGGAAGGGGCGCGCAgggg                                                                          >  2:236080/1‑150 (MQ=255)
                                                                                 cgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTTCGCAC‑CGGGGTGCGCCCCCCTTAAT‑TGCCTCGCCCTCTAAATTG‑AATGGGGGCGGGGGGGCGCCggggggggg                                                                     >  2:3870479/1‑149 (MQ=255)
                                                                                  gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGCTT‑AGCAG‑CGGGGTTCG‑CCCCCCTAATGTGCCTCCCCCTATAAAATGTAATGG‑GCAGGGGGGGCGCCGGGGGGGGcc                                                                    >  2:946660/1‑150 (MQ=255)
                                                                                         cATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGCTTCCGCAG‑CGGGGTGCG‑CTCCCCTTATTTGCCTCGCCCTATAAATTT‑AATGGTGGAGGGGGGGCGCCGGGGGGGGCCCAAcgcc                                                             >  2:4088092/1‑150 (MQ=255)
                                                                                                            ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTCAGCAA‑CGGGGTGCG‑CTCCCCTTATGTGCCCCCCCCTATAAATTT‑AATTGGGCCGGGAGCGCGCCGGGGGGGGCCCATCCCCCCCCCCCCCCCCCGT‑CCCg                                         >  2:324072/1‑150 (MQ=255)
                                                                                                             gTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTTGTTTTGCAG‑CGGGGTGGG‑CTCCCCTTATTTGGCTCGCCCTTTAAAATT‑GATTGTGGAGGGGGGGCGCCGGGGGGGGGCAAACCCCCCCGCCCCCCCCTGT‑tcccc                                        >  2:3693083/1‑148 (MQ=255)
                                                                                                              ttGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTTGGTTTAGCAG‑CGGGGTGCG‑CTCCCCTTATGTGGCTCGCCCTATAAATTT‑AATGGGGCAGGGGGCGCGCCGGGGGGGGGCAAACGCCCCCCCCCCCCGCCGT‑CCCCGc                                       >  2:563027/1‑150 (MQ=255)
                                                                                                                 cAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTGGGTTCCGCAA‑CGGGGTTCG‑CTCCCTTAATGTGGCTCGCCCCATAAAATT‑AATGGTGCAGGGGGCGCGCAGGGGGGGGCCAATCCCCCCCGCCCCCCGCTCG‑CCCCGccct                                    >  2:1735477/1‑148 (MQ=255)
                                                                                                                     aaaGCTAAGGGACTTAGCCTGCGGCGG‑TTTTTTTTGGCTTCACCCG‑CGGGGTGAG‑CTCCCCTAATTTGGCCCCGCATATAAATTT‑AATGGGGCAGGGGGCGCGCCGGGGGCGGCCCAACGCCGCCGCCCCCCTCTGTCCCCGGCCTTggg                                 >  2:2324751/1‑150 (MQ=255)
                                                                                                                      aaGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCgggg                               <  1:3392055/150‑1 (MQ=255)
                                                                                                                        gCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGaa                             <  1:1632717/150‑1 (MQ=255)
                                                                                                                          tAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACg                           <  1:2920597/150‑1 (MQ=255)
                                                                                                                                aCTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAg                     <  1:2072621/150‑1 (MQ=255)
                                                                                                                                 cTTAGCCTGGGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGc                    <  2:2932439/150‑1 (MQ=255)
                                                                                                                                  ttAGCCTGCGGCGG‑TTTTGTTTGGCTGCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCg                   <  2:2463075/150‑1 (MQ=255)
                                                                                                                                    aGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGAt                 <  1:3875855/150‑1 (MQ=255)
                                                                                                                                     gCCTGCGGCGG‑TTTTGTTTGGCTTCGGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGAtt                <  2:3808216/150‑1 (MQ=255)
                                                                                                                                        tGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTg             <  2:2040886/150‑1 (MQ=255)
                                                                                                                                            gcgg‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGc         >  1:2319848/1‑150 (MQ=255)
                                                                                                                                              gg‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca       >  1:2478761/1‑150 (MQ=255)
                                                                                                                                              gg‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca       >  2:3301444/1‑150 (MQ=255)
                                                                                                                                               g‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac      >  2:6398/1‑150 (MQ=255)
                                                                                                                                                    tgtttgGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAac  <  1:4249297/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
GCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCG‑CTCCCTTAATGTGCCTCGCCATATAAATTG‑AATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAAC  >  AP012306/934906‑935202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑