Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT24.2% 644.7 / 136.7 277S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (17/193);  new base T (67/0);  total (84/193)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 333 total aligned reads displayed.

ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  AP012306/3938738‑3939035
                                                                                                                                                      |                                                                                                                                                     
 cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttct                                                                                                                                                      <  1:556441/150‑1 (MQ=255)
  tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                                     >  1:1658987/1‑150 (MQ=255)
   cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCtttttttttt                                                                                                                                                    >  2:3807287/1‑150 (MQ=255)
    aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                                   <  1:1213935/150‑1 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                  <  1:1256175/150‑1 (MQ=255)
      aGAAAAATGCGTCATGCGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                 <  2:1987536/150‑1 (MQ=255)
      aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCttttttttttttt                                                                                                                                                 >  1:104690/1‑149 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTTGtttttttttttttt                                                                                                                                               >  2:1307227/1‑147 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAttttttttttttttt                                                                                                                                               >  1:1361601/1‑147 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                               <  2:2604860/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                               <  1:2079918/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                               <  2:1895601/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                               <  2:1477873/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGACGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                               <  2:2646302/150‑1 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttc                                                                                                                                              >  2:79619/1‑146 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttc                                                                                                                                              >  2:229535/1‑146 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAttttttttttttttttc                                                                                                                                             >  2:1117522/1‑145 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                                                                                             <  2:653210/150‑1 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCACTTTTTTTTTTTTTGCCAt                                                                                                                                           >  1:3910191/1‑150 (MQ=255)
             tGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg                                                                                                                                          <  2:2237281/150‑1 (MQ=255)
             tGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg                                                                                                                                          <  1:3265388/150‑1 (MQ=255)
              gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                         <  1:2232766/150‑1 (MQ=255)
              gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                         <  1:707087/150‑1 (MQ=255)
                gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                       <  1:1929670/150‑1 (MQ=255)
                gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                       <  1:443292/150‑1 (MQ=255)
                gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                       <  1:2700895/150‑1 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgagca                                                                                                                                      >  1:735253/1‑148 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCAttggaa                                                                                                                                      >  2:1254524/1‑145 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTCTTCCATgagga                                                                                                                                      >  2:3672744/1‑148 (MQ=255)
                   aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGt                                                                                                                                    >  1:3543766/1‑150 (MQ=255)
                   aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATgaggaga                                                                                                                                    >  1:4172967/1‑149 (MQ=255)
                   aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                                    <  2:4078486/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                               <  2:2830777/150‑1 (MQ=255)
                         agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                              <  1:547410/150‑1 (MQ=255)
                           taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTTCCATGAGAAGGGAGCGGag                                                                                                                            >  1:405499/1‑150 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                          <  1:2461007/150‑1 (MQ=255)
                               ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                                                                                        <  1:3388510/150‑1 (MQ=255)
                                   ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                                                                                   >  1:3751669/1‑119 (MQ=255)
                                   ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                    <  2:722089/150‑1 (MQ=255)
                                    tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGtt                                                                                                                   >  1:1711970/1‑150 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  1:3370109/150‑1 (MQ=255)
                                       gagTTTTTTTAGAAAGATTGCAGGTAACCATTAAAAGGCTCC‑TGCCTAATCCGGTCTGGGTTAATTTTAAGAAGCCATGTCGGGGGTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                                <  2:460432/150‑1 (MQ=255)
                                       gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGAGTATc                                                                                                                >  1:2769786/1‑150 (MQ=255)
                                        agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGAAGGGAGAGCGGAGAGGGGTTAtat                                                                                                               >  2:356192/1‑148 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGAGAGCGGAGAGGGGGGTTATCTTg                                                                                                             >  2:2464347/1‑150 (MQ=255)
                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGc                                                                                                            >  1:3467663/1‑150 (MQ=255)
                                             aTTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                                          <  1:175683/150‑1 (MQ=255)
                                                cAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgac                                                                                                       <  1:4020345/150‑1 (MQ=255)
                                                cAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgac                                                                                                       <  1:2096934/150‑1 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGCTTTTTTTTTTTTTTTTCATGAGAAGGGAGGGCAGAGAGGGGGTATATTTCctgtct                                                                                                      >  2:1729594/1‑150 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAAGTGAGCGCAGAGAGGGGGTTTTTTTCctgact                                                                                                      >  2:2177536/1‑150 (MQ=255)
                                                    aaGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTCAGAAGTGGGCGCCGAAAGGGATTTTTTTTCCTGCCTGaa                                                                                                   >  1:822060/1‑150 (MQ=255)
                                                      gTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                                                                                 <  2:3313324/150‑1 (MQ=255)
                                                       tttGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTg                                                                                                <  1:3657786/150‑1 (MQ=255)
                                                         tGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                              <  2:4125614/150‑1 (MQ=255)
                                                          gCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTACCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCg                                                                                             <  1:2520547/150‑1 (MQ=255)
                                                              gTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTa                                                                                         <  1:3941722/150‑1 (MQ=255)
                                                              gTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTa                                                                                         <  1:3033563/150‑1 (MQ=255)
                                                               tAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTaa                                                                                        <  2:1083607/150‑1 (MQ=255)
                                                               tAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTaa                                                                                        <  2:3870677/150‑1 (MQ=255)
                                                                 aCCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑t                                                                                     <  2:1372676/150‑1 (MQ=255)
                                                                     ttATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCAAGAGGAGGGAGCGGAGGGAGGGGGTATTTTTCCTGACTGAACTTGCGGTTTAC‑ttttc                                                                                 >  2:2897738/1‑148 (MQ=255)
                                                                     ttATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTcc                                                                                 <  1:2194176/150‑1 (MQ=255)
                                                                      tataA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCcg                                                                                <  2:2063175/150‑1 (MQ=255)
                                                                        taA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAAGGGAGCGCAGGGAGGGAGTATCTTTCCTGACTGAACTGGCGGTTTAG‑TTTTcgcg                                                                              >  2:2801179/1‑150 (MQ=255)
                                                                         aa‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGt                                                                             <  1:1454420/150‑1 (MQ=255)
                                                                         aa‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGt                                                                             <  1:3074083/150‑1 (MQ=255)
                                                                                cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCgg                                                                       <  1:2901532/150‑1 (MQ=255)
                                                                                  tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCAGGGGGGGGTTATTTTTCCTGACTGAACTGGCGGTTTAG‑TTTTCGCGTACCCcgcg                                                                     >  2:3952072/1‑150 (MQ=255)
                                                                                      tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTGCCTTAGAAGTGAGGGCAGGGGGGGGATTTTTTTCCTTACTGAAATTGCGGTTTAG‑TTTTCGCGTACCCGGCGgttt                                                                 >  2:4264946/1‑149 (MQ=255)
                                                                                       ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAg                                                                <  2:3423530/150‑1 (MQ=255)
                                                                                       ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAg                                                                <  1:2929528/150‑1 (MQ=255)
                                                                                        taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCCTGAGAAGTGAGCGGAGAGAGGGGTTATCTTGCCTGACTTAACTGGCGCTTTAGTTTTCCCCGACCCCGGGGtttgt                                                                >  2:2409318/1‑148 (MQ=255)
                                                                                         aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGtt                                                              <  1:2329630/150‑1 (MQ=255)
                                                                                          ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCAGAGAGGGGGTATTTTTCCTTGATGAAATTGCGGGTAAG‑TTTTCGCGTACCCCGCGGTTTGTTa                                                             >  2:3479595/1‑150 (MQ=255)
                                                                                                  gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                     <  2:1565191/150‑1 (MQ=255)
                                                                                                       aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                                <  1:4106417/150‑1 (MQ=255)
                                                                                                       aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                                <  1:229535/150‑1 (MQ=255)
                                                                                                           cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGCCCTGAGAAGTGGGGGCAGGGAGGGGGTATCTTGCCTGCCTGGAATGGCGGTTTAA‑TTTTCGCGTACCCGGCGGTTTGTTATTGCGGGTGATGGTAtt                                            >  2:2565073/1‑150 (MQ=255)
                                                                                                             ggATGCCATGTCGCGGATCACGTTTGCAGCGGTCAGTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGAGATTAAG‑TTTCCGCGTCCCCGGCGTTTAGTTATTGCGGATGATGATATTGa                                          <  2:1780760/150‑1 (MQ=255)
                                                                                                              gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                         <  2:2046153/150‑1 (MQ=255)
                                                                                                              gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                         <  2:1821421/150‑1 (MQ=255)
                                                                                                               aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                        <  2:3631274/150‑1 (MQ=255)
                                                                                                                 gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCTTCAGGAGAGGGCGCAGTGAGGGGTTATTTTGCCTGGATTAACTGGGGGTTTAG‑CTTCCGCGTAACCCGCGGTTTGTTATTGTCGATTGTGATAttttaacc                                      >  2:3284380/1‑144 (MQ=255)
                                                                                                                      gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                 <  2:4236724/150‑1 (MQ=255)
                                                                                                                         gcgGATCTCGTTTGCGGCGGTAATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                              <  2:1689333/150‑1 (MQ=255)
                                                                                                                           ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGTCTGAACTGGCGATTAAG‑TTTTCGCGTACCCCGCGTTTTGTTATTTCGGGTGATGGTATTTAAGCTCCGCTGCTTg                            >  2:2219516/1‑150 (MQ=255)
                                                                                                                               ctcGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGGTTAGTTATTTCGGGTGATGATATTGAAGCTCGGCTGATTGGTTc                        >  2:3906812/1‑150 (MQ=255)
                                                                                                                               ctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                        <  1:1774367/150‑1 (MQ=255)
                                                                                                                                       cggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc                <  2:95871/150‑1 (MQ=255)
                                                                                                                                        ggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGcc               <  1:3122910/150‑1 (MQ=255)
                                                                                                                                          cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAGGCTCGGCTGATTGGTTCATTGTCGCCAt             <  2:909291/150‑1 (MQ=255)
                                                                                                                                             tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          >  1:4305116/1‑150 (MQ=255)
                                                                                                                                             tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          <  1:4246721/150‑1 (MQ=255)
                                                                                                                                               aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTTATTGGTTCATTGTCGCCATCACCg        <  2:617457/150‑1 (MQ=255)
                                                                                                                                                ttttttCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       <  1:624544/150‑1 (MQ=255)
                                                                                                                                                 tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt      <  1:2043590/150‑1 (MQ=255)
                                                                                                                                                 tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt      >  1:2576356/1‑150 (MQ=255)
                                                                                                                                                  ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg     <  2:4023025/150‑1 (MQ=255)
                                                                                                                                                   tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg    >  2:3159422/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAA‑GCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  AP012306/3938738‑3939035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑