Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG30.0% 180.3 / 30.5 111G60G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (17/60);  new base G (33/0);  total (51/60)
Fisher's exact test for biased strand distribution p-value = 7.82e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 185 total aligned reads displayed.

AAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGC  >  AP012306/341230‑341525
                                                                                                                                                    |                                                                                                                                                     
 aaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGaa                                                                                                                                                    >  1:2777929/1‑150 (MQ=255)
   gCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGaaaa                                                                                                                                                  <  2:1977864/150‑1 (MQ=255)
    cTGGCGCAACGAGGACGTTTTTCCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGaaaaa                                                                                                                                                 <  2:754706/150‑1 (MQ=255)
     tGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGaaaaaa                                                                                                                                                >  2:2764367/1‑149 (MQ=255)
      ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaaa                                                                                                                                               >  1:3584399/1‑148 (MQ=255)
       gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaaac                                                                                                                                              >  2:3728845/1‑147 (MQ=255)
        cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGGGTATGGC‑GGGGGGAAAAgagg                                                                                                                                             >  2:3437270/1‑147 (MQ=255)
        cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCa                                                                                                                                             >  1:2167170/1‑150 (MQ=255)
         gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAACCaa                                                                                                                                            >  1:2129862/1‑150 (MQ=255)
         gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCaa                                                                                                                                            <  1:3460626/150‑1 (MQ=255)
         gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCaa                                                                                                                                            <  2:4288468/150‑1 (MQ=255)
         gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCaa                                                                                                                                            >  1:4077130/1‑150 (MQ=255)
           aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAg                                                                                                                                          <  1:1980836/150‑1 (MQ=255)
            aCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAgg                                                                                                                                         <  1:4115245/150‑1 (MQ=255)
               aGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCaaaagggg                                                                                                                                      >  2:3270877/1‑145 (MQ=255)
                ggACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCg                                                                                                                                     <  2:1112032/150‑1 (MQ=255)
                   cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGAcc                                                                                                                                  <  2:1419695/150‑1 (MQ=255)
                   cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGAcc                                                                                                                                  <  1:314555/150‑1 (MQ=255)
                    gTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCg                                                                                                                                 <  1:2628352/150‑1 (MQ=255)
                     ttttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATAGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAAACAAAAGGGGCGcccc                                                                                                                                 >  2:3058839/1‑149 (MQ=255)
                      tttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGTGCGACCGcc                                                                                                                               >  1:1133045/1‑150 (MQ=255)
                        tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTa                                                                                                                             <  2:3488723/150‑1 (MQ=255)
                        tACCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAATAAACCAAAAGTGGGGACGCCtg                                                                                                                             >  2:1051043/1‑149 (MQ=255)
                             cGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGGGCGACCCCCCATTT‑ca                                                                                                                       >  1:667401/1‑150 (MQ=255)
                             cGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCtctcttc                                                                                                                        >  1:1854892/1‑144 (MQ=255)
                              gCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑cac                                                                                                                      <  1:398829/150‑1 (MQ=255)
                               cATCCCACCCCGAATGTGGGGTGCGGCATTGTCAAAGAGGGCGAAATTGTCGGTGAGGGTTCCCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑caca                                                                                                                     <  2:49382/150‑1 (MQ=255)
                                 tCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACt                                                                                                                   <  1:1063883/150‑1 (MQ=255)
                                     aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCAAAGG‑GCGGCCGCCCCTGT‑CACACTCGaa                                                                                                               >  2:247544/1‑150 (MQ=255)
                                      aCCCGAATGTGGGGTGCGTCATTGTCAAAGATGGCGAAATTGTGGGTGAAGTTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAAc                                                                                                              <  1:3449002/150‑1 (MQ=255)
                                        ccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCCAAAGGGCGACCCCCCATTT‑CACACTCCAAccc                                                                                                            >  1:3971455/1‑150 (MQ=255)
                                         cGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTGGGTGAAGGTTCCCCCCAGCGTGCGGGTGACCCATATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTGAAAAAGCAAAAGGGGGGCCCGCCTATGT‑CACACTCGAACCCt                                                                                                           <  2:1714502/150‑1 (MQ=255)
                                         cGAATGCCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTCCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGT‑CACACTCGAACCCt                                                                                                           <  2:2416525/150‑1 (MQ=255)
                                          gAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCGCCCATGT‑CACACTCGAACCCTg                                                                                                          >  1:56471/1‑150 (MQ=255)
                                          gAATGTCGGGTGCGTCATTGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTCCCACCAAGGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTg                                                                                                          <  2:1179763/150‑1 (MQ=255)
                                           aaTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCCCCCCTGT‑CACACTCGCAccccct                                                                                                         >  1:2177644/1‑147 (MQ=255)
                                             tGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAg                                                                                                       <  1:2333982/150‑1 (MQ=255)
                                             tGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAg                                                                                                       <  1:1182689/150‑1 (MQ=255)
                                              ggcGGGTCGGTCATTGGCAAAGAGGGAGAAATTGTGGGTGAGGTTTACCACCACCGTGGGGGTGAACCACATCCCGAGGTCCACGCGTGGCGTGGGGG‑GGGTGAAAAACCCAAAGGTGCGCCCGCCTATGT‑CACACTCGAACCCTGTAGc                                                                                                      <  2:282559/148‑1 (MQ=255)
                                              gTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGTGCGACCGCCCATGT‑CACACTCGAACCCCGTAGc                                                                                                      >  1:1764819/1‑150 (MQ=255)
                                                cGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCCCTTT‑TACACCCCAACCCCGTAAccc                                                                                                    >  2:2517635/1‑149 (MQ=255)
                                                 gggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCcat                                                                                                   >  1:68204/1‑150 (MQ=255)
                                                 gggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGT‑CACACTCCAACCCTGTAGCcat                                                                                                   >  1:1910719/1‑150 (MQ=255)
                                                   gTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCcatca                                                                                                 <  2:1772611/150‑1 (MQ=255)
                                                      cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATgg                                                                                              <  1:128466/150‑1 (MQ=255)
                                                       gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAGCCAAAAGTGCGACCGCCTATGT‑CACACTCGAACCCCGTAGCCATCATGGt                                                                                             >  1:4061942/1‑150 (MQ=255)
                                                        tCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCCATGT‑CACACTCCAACCCCCTAGCCATCATGGTc                                                                                            >  2:4209238/1‑150 (MQ=255)
                                                           ttGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTAACCCAAATGCCGAAGCCCACGCGTTGGGTATGGG‑GGGTGAAAAAGCCAAAGGTGGGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTa                                                                                         <  2:3607855/150‑1 (MQ=255)
                                                              tCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGc                                                                                      <  1:3270877/150‑1 (MQ=255)
                                                              tCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGc                                                                                      >  1:2676298/1‑150 (MQ=255)
                                                              tCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCCCCCATGT‑CACACCCCAACCCCGTAGCCACCATGGTCGTACGc                                                                                      >  2:3884074/1‑150 (MQ=255)
                                                               cAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGG‑GGAAAAGCCAAAGGAGCGACCGCCTCTGT‑TCCACCCGCACCCCGTAACCATCCTGGGCGTACGccc                                                                                    >  2:881783/1‑149 (MQ=255)
                                                                aaaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAGCCAAAAGTGCGGCCCCCCAAGTCCACCCCCGACCCCT‑CAGCCCGCATCGTCGTACCCCa                                                                                    >  2:3803597/1‑150 (MQ=255)
                                                                 aaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAAACAAAAGGGGGGCCGGCCCA‑GT‑CACACTCGAAACCCGTAGCCATAATGGTGGGACGCCAc                                                                                   >  2:1919357/1‑150 (MQ=255)
                                                                       ccGAAATTGTCGGTGAAGGTTCCCCCCACCGTGCGGGGGAACCACCGGCCGAAGTACCCGCGTGGCTTATGGC‑GGGTAAAAAAGCAAAAGGGGCAACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCt                                                                             <  2:2205575/149‑1 (MQ=255)
                                                                             tggTCGGTGAAGGTTACCACCAACGTGGGGGTGAACCACTTGCCGAAGTACCCGCGTTGCGTATGGG‑GGGTAAAAAACCCAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg                                                                       <  2:635056/148‑1 (MQ=255)
                                                                               gTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCCAAAGTGCGGCCCCCCCTGT‑CCCACCCGCACCCCCTAGCCATCATGGTCGTACGCCACCGCGCTGTGgcgcc                                                                     >  2:391236/1‑149 (MQ=255)
                                                                                  ggTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCAAAGG‑GCGACCGCCCATGT‑CACACTCGAACCCCGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTc                                                                  >  2:521296/1‑150 (MQ=255)
                                                                                  ggTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGGGCGACCGCCCATGT‑CACACTCCCACCCCGTAGCCATCATGGTCGTACGCCACCCGGCTGTGACGCACTc                                                                  >  2:207805/1‑150 (MQ=255)
                                                                                   gTGAGGGTTCCCACCACGGTGGGGGTGAACCACATGCCAAAGTACACGCGTGGCGTATGGC‑GGGTAAAAAAGCAAAGGGTGCGCCCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCa                                                                 <  2:2264047/150‑1 (MQ=255)
                                                                                   gTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCa                                                                 <  1:1360492/150‑1 (MQ=255)
                                                                                     gAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGC‑GGGTAAAAAAGCCAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                               <  1:3028167/150‑1 (MQ=255)
                                                                                          ttACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGGGCGACCCCCCATGT‑CACACTCGAACCCCGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCaccccccc                                                          >  2:3274476/1‑143 (MQ=255)
                                                                                            gccaccaTGGTGGGGGGCAACCACGTCCGGAACTACCCGGGTTGCGTAGGGC‑GGGTGAAAAAGCAAAGGGTGCAACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTg                                                        <  2:2654719/149‑1 (MQ=255)
                                                                                            ccccccaGCGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTg                                                        <  2:1946864/146‑1 (MQ=255)
                                                                                            cccaccaACGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGGAAAAAAGCCAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTg                                                        <  2:2565405/149‑1 (MQ=255)
                                                                                             ccaccaAGGGGCGGGTAAACCACATGCCGAAGTACCCGCGTGGCGTAGGGG‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTgg                                                       <  2:1018992/150‑1 (MQ=255)
                                                                                                ccaACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGt                                                    >  1:298007/1‑150 (MQ=255)
                                                                                                   aCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCTATGT‑CACACTCGAACCCCGTAGCCATCATGGTCGTACGCCCCCCTGCTGTGACGCACTCATCGCCCCTGGCggcgg                                                 >  2:480555/1‑146 (MQ=255)
                                                                                                       gCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACCGCCCATGT‑CACACCCCAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCCGGCGCGgcgcgc                                             >  2:299624/1‑150 (MQ=255)
                                                                                                       gCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCCCCTATGT‑CACACTCCAACCCCGTAGCCATCATTGGCGTACGCCACCCGGCTGTGACGCACTCATCGCCCCCGGGGGAgcgcgc                                             >  2:962148/1‑150 (MQ=255)
                                                                                                          ggTGAACCAAATGCAGAAGTACCGGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTg                                          <  1:74990/150‑1 (MQ=255)
                                                                                                            tGAACCACATGCCGAAGTACACGCGTGGCTTAGGGC‑GGGTAAAAAAGCCAAAGTTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGt                                        <  1:3779069/150‑1 (MQ=255)
                                                                                                             gAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATAGCCGCTGGCGTAGCGCGCGTGGtt                                       <  2:3727169/150‑1 (MQ=255)
                                                                                                             gAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGtt                                       <  1:1947900/150‑1 (MQ=255)
                                                                                                              acccACTTCCCGAAGTACAGGCGTGGCGTAGGGC‑GGGTGAAAAGCCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTg                                      <  2:3172581/148‑1 (MQ=255)
                                                                                                               aCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGTGCGACCCCCCATGT‑CACACTCGAACCCCGTAGCCATCATGGGCGTACGCCACCCTGCTGTGACGCCCTCCTCGCCGCTGGCGTAGCGCGCGTGGTTgg                                     >  2:41456/1‑149 (MQ=255)
                                                                                                                ccACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTAAAAAACCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGcc                                    <  2:1854892/150‑1 (MQ=255)
                                                                                                                  atgtGCCGAATTACCGGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                  <  1:4211396/147‑1 (MQ=255)
                                                                                                                  acaTGCCGAAGTACACGCGTTGCGTATGGG‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                  <  2:1233474/150‑1 (MQ=255)
                                                                                                                   caTGCCGAAGTCCACGCGTTGCGTATGGC‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCg                                 <  2:3852240/150‑1 (MQ=255)
                                                                                                                    aTGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGa                                <  1:3571558/150‑1 (MQ=255)
                                                                                                                    aTGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGGGACCCCCCATGT‑CCCACCCCAACCCCGTAGCCATCATGGTCGTACCCCCCCCCGCTGTGACGCACCCCTCGCCGCCGGCGGAGCGCGCCTGGTTGccccca                                >  2:2114286/1‑146 (MQ=255)
                                                                                                                     tccagAAGTACCCGCTTTCCTTAGGGG‑GGGTGAAAAGGCAAGGGGTGGGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGAt                               <  2:1995756/146‑1 (MQ=255)
                                                                                                                      cccGAAGTACACGCGTGGCGAATGGG‑GGGTGAAAAAGCCAAAGGTGCAACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATg                              <  1:3649175/149‑1 (MQ=255)
                                                                                                                       ccGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGACAAAAGTGCGGCCCCCCCTTT‑CACACTCGGACCCCGTAACCCTCATGGTCGGACGCCCCCCCGCTGTGGCGCACTCCTCGCCCCTGGCGTAGGGCGCGTGGTTGCCTCGATGc                             >  2:2944451/1‑150 (MQ=255)
                                                                                                                          agaaaaacGGGTTGCGTATGGG‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAg                          <  2:1486538/144‑1 (MQ=255)
                                                                                                                           aGTCCACGCGTTGCGTATGGC‑GGGGGAAAAAGCAAAGGGTGGGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGa                         <  1:2441191/150‑1 (MQ=255)
                                                                                                                           aGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGa                         <  2:2904557/150‑1 (MQ=255)
                                                                                                                            gTACACGCGTTGCGTATGGC‑GGTTAAAAAGGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGAt                        <  1:786923/150‑1 (MQ=255)
                                                                                                                            gTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGAt                        <  1:3720540/150‑1 (MQ=255)
                                                                                                                                 cgcgTGGCGTATGGC‑GGGTAAAAAGGCAAAAGGGGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTaa                   <  1:1582508/150‑1 (MQ=255)
                                                                                                                                     ttcggTAGGGC‑GGGAAAAAAAGAAAAGGGCGCGGCCGCCTATGT‑CACACTCGAGCCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCg               <  2:674202/146‑1 (MQ=255)
                                                                                                                                      tgggTATGGG‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGc              <  1:1012497/147‑1 (MQ=255)
                                                                                                                                        cGTATGGG‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAg            <  2:3971455/150‑1 (MQ=255)
                                                                                                                                            tGGC‑GGTTCAAAAAGCAAAAGGTGCACCCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCg        <  2:463619/150‑1 (MQ=255)
                                                                                                                                            agggggggTAAAAAAGCCAAGGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTc         <  2:3537733/145‑1 (MQ=255)
                                                                                                                                               c‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTg     <  1:749305/150‑1 (MQ=255)
                                                                                                                                                ggggTAAAAAAGCAAAGGGGGCGCCCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTg     <  2:3415323/149‑1 (MQ=255)
                                                                                                                                                ggggTAAAAAAGCAAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTg     <  2:3989682/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGT‑CACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGC  >  AP012306/341230‑341525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑