Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,714,5550TG21.9% 706.3 / 20.2 279T175P (ACC→CCC) ygjVconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (95/123);  new base G (61/0);  total (156/123)
Fisher's exact test for biased strand distribution p-value = 6.26e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 319 total aligned reads displayed.

CGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTG  >  AP012306/2714407‑2714704
                                                                                                                                                    |                                                                                                                                                     
 gTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGttt                                                                                                                                                     >  1:1808577/1‑150 (MQ=255)
 gTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGgtt                                                                                                                                                     >  1:1539522/1‑150 (MQ=255)
  tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGtttt                                                                                                                                                    <  1:4233830/150‑1 (MQ=255)
   aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTtc                                                                                                                                                   >  2:2547363/1‑150 (MQ=255)
      gCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATATCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAAGAGGTTTCTCa                                                                                                                                                >  2:1888533/1‑150 (MQ=255)
          gACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACtt                                                                                                                                            <  2:106489/150‑1 (MQ=255)
          gACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACtt                                                                                                                                            <  1:4139497/150‑1 (MQ=255)
          gACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAAcct                                                                                                                                            >  2:241179/1‑148 (MQ=255)
           aCTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACttt                                                                                                                                           >  1:2288635/1‑150 (MQ=255)
             tctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACtttta                                                                                                                                         >  1:2036872/1‑148 (MQ=255)
             tctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTaa                                                                                                                                         >  2:3667550/1‑150 (MQ=255)
               ttGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAAc                                                                                                                                       >  2:4069623/1‑150 (MQ=255)
                 gCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACgg                                                                                                                                     >  1:2241870/1‑150 (MQ=255)
                   gAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGAt                                                                                                                                   >  1:101365/1‑150 (MQ=255)
                   gAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGAt                                                                                                                                   >  2:2244765/1‑150 (MQ=255)
                   gAAACCGCGCTGAACTCTTCAACGGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGAt                                                                                                                                   <  2:316832/150‑1 (MQ=255)
                    aaaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATc                                                                                                                                  <  1:3711166/150‑1 (MQ=255)
                    aaaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATc                                                                                                                                  <  2:3306175/150‑1 (MQ=255)
                    aaaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTTTCAACTTTAAACGGATc                                                                                                                                  >  2:2745832/1‑150 (MQ=255)
                     aaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCa                                                                                                                                 >  1:1891348/1‑150 (MQ=255)
                     aaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCa                                                                                                                                 <  1:1706280/150‑1 (MQ=255)
                         gcgcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTc                                                                                                                             <  2:1008614/150‑1 (MQ=255)
                          cgcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTcc                                                                                                                            <  1:4245993/150‑1 (MQ=255)
                             tGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAAAGGATCAATTCCCCt                                                                                                                         >  2:156666/1‑150 (MQ=255)
                                    ttCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGc                                                                                                                  <  1:1177452/150‑1 (MQ=255)
                                     tCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCa                                                                                                                 <  2:1840423/150‑1 (MQ=255)
                                       aaCTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATc                                                                                                               <  2:954493/150‑1 (MQ=255)
                                       aaCTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATc                                                                                                               >  1:1100494/1‑150 (MQ=255)
                                           gACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACAGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGcc                                                                                                           >  1:3996751/1‑150 (MQ=255)
                                            aCGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCa                                                                                                          <  1:2807225/150‑1 (MQ=255)
                                                gCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAAGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCCCCAGaaa                                                                                                      >  2:665068/1‑150 (MQ=255)
                                                 cTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAAc                                                                                                     <  2:3624271/150‑1 (MQ=255)
                                                   gTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCCGAAACGa                                                                                                   >  2:218596/1‑150 (MQ=255)
                                                    ttCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGaa                                                                                                  >  1:504376/1‑150 (MQ=255)
                                                           cGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATAtt                                                                                           <  1:3554290/150‑1 (MQ=255)
                                                           cGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATAtt                                                                                           <  2:2224855/150‑1 (MQ=255)
                                                            ggCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTc                                                                                          <  1:2684420/150‑1 (MQ=255)
                                                                gCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGc                                                                                      >  1:2995382/1‑150 (MQ=255)
                                                                 cTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGcc                                                                                     <  1:1065055/150‑1 (MQ=255)
                                                                   tGCCAGGCAGAAGATGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCAt                                                                                   <  1:3309693/150‑1 (MQ=255)
                                                                    gCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCAtt                                                                                  <  1:2305835/150‑1 (MQ=255)
                                                                      cAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCAACCGCCCGAAACGAATGATATTCAGGGCATTCa                                                                                >  2:3002660/1‑150 (MQ=255)
                                                                        ggCAGAAGCCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATa                                                                              <  2:244188/150‑1 (MQ=255)
                                                                          cAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAg                                                                            <  2:1271668/150‑1 (MQ=255)
                                                                          cAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAg                                                                            <  1:428351/150‑1 (MQ=255)
                                                                          cAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAg                                                                            >  1:3034206/1‑150 (MQ=255)
                                                                           agaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTTTCAACTTTAAACGGTTCAATTCCCCTTTTCTGCATCCGCCCGAAACGAATGGTATTAAGGCCACTCATAAGc                                                                           >  2:825932/1‑150 (MQ=255)
                                                                            gaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCa                                                                          <  1:66725/150‑1 (MQ=255)
                                                                              agaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGTAAGGGGTTTTCTGAACTTTGAACTGGGCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTTAGGCCATTCTTTAGctgt                                                                        >  2:604794/1‑147 (MQ=255)
                                                                               gaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaa                                                                       <  1:1229960/150‑1 (MQ=255)
                                                                               gaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaa                                                                       >  1:908812/1‑150 (MQ=255)
                                                                                 cGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa                                                                     <  2:126014/150‑1 (MQ=255)
                                                                                 cGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa                                                                     <  1:3450211/150‑1 (MQ=255)
                                                                                      gTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTAcc                                                                >  1:3200796/1‑150 (MQ=255)
                                                                                      gTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTAcc                                                                <  1:3929015/150‑1 (MQ=255)
                                                                                      gTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTAcc                                                                >  2:2688581/1‑150 (MQ=255)
                                                                                       tCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTAccc                                                               <  1:1968576/150‑1 (MQ=255)
                                                                                           gCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCaa                                                           >  1:3104609/1‑150 (MQ=255)
                                                                                            cAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAat                                                          <  1:2817671/150‑1 (MQ=255)
                                                                                            cAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAat                                                          >  1:3403726/1‑150 (MQ=255)
                                                                                             aaaTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatc                                                         >  1:379892/1‑150 (MQ=255)
                                                                                              aaTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatca                                                        >  1:1375414/1‑150 (MQ=255)
                                                                                               aTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatcat                                                       <  1:3531019/150‑1 (MQ=255)
                                                                                               aTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatcat                                                       >  1:2841864/1‑150 (MQ=255)
                                                                                                tAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatcatc                                                      <  2:3110495/150‑1 (MQ=255)
                                                                                                tAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatcatc                                                      <  2:4177051/150‑1 (MQ=255)
                                                                                                tAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatcatc                                                      >  2:4242040/1‑150 (MQ=255)
                                                                                                   cgcCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTg                                                   <  1:3366248/150‑1 (MQ=255)
                                                                                                    gcCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTTTCAACTTTCAACGGATCCATTCCCCTTTTCTGCATCCGCCAGAAAAGCATGATATTCAAGCCATTCATAAGCAGAAAACTACCCTCAATCATCGttc                                                  >  2:1700867/1‑148 (MQ=255)
                                                                                                    gcCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgc                                                  >  2:3777171/1‑150 (MQ=255)
                                                                                                    gcCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgc                                                  >  2:3528979/1‑150 (MQ=255)
                                                                                                     cccTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgcc                                                 <  2:2337979/150‑1 (MQ=255)
                                                                                                      ccTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgccg                                                <  2:323674/150‑1 (MQ=255)
                                                                                                        tGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTgccgcc                                              >  2:1961042/1‑150 (MQ=255)
                                                                                                         gCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATCCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCt                                             <  2:1379954/150‑1 (MQ=255)
                                                                                                            taattaatTGTTTAACCCCTTTCGTCTACGGCGGGAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAAAGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATc                                          >  1:2422836/1‑150 (MQ=255)
                                                                                                             aattaattGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTTTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCg                                         >  2:3024689/1‑150 (MQ=255)
                                                                                                                 aattGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAccc                                     >  2:12042/1‑150 (MQ=255)
                                                                                                                 aattGTTTAACCCCTTTCGTCTACGGCGGAAAGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAccc                                     >  2:2022422/1‑150 (MQ=255)
                                                                                                                  attGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAcccc                                    <  2:2088924/150‑1 (MQ=255)
                                                                                                                     gTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGcc                                 >  2:2172102/1‑150 (MQ=255)
                                                                                                                     gTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGcc                                 <  1:2143881/150‑1 (MQ=255)
                                                                                                                     gTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGcc                                 >  1:1594421/1‑150 (MQ=255)
                                                                                                                      tttAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGccc                                >  2:1812744/1‑150 (MQ=255)
                                                                                                                          aCCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGaa                            >  1:1387758/1‑150 (MQ=255)
                                                                                                                           ccccTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAg                           >  1:2533103/1‑150 (MQ=255)
                                                                                                                             ccTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGtt                         <  1:2762741/150‑1 (MQ=255)
                                                                                                                               tttCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTtgt                       >  2:146627/1‑150 (MQ=255)
                                                                                                                                  cGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGaa                    >  2:293102/1‑150 (MQ=255)
                                                                                                                                  cGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGaa                    >  2:2877464/1‑150 (MQ=255)
                                                                                                                                     cTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCa                 >  1:1467860/1‑150 (MQ=255)
                                                                                                                                     cTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCa                 >  2:2647992/1‑150 (MQ=255)
                                                                                                                                          gcggAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAg            >  2:76049/1‑150 (MQ=255)
                                                                                                                                            ggAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCa          >  2:4279403/1‑150 (MQ=255)
                                                                                                                                              aaGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAac        <  2:4152026/150‑1 (MQ=255)
                                                                                                                                               aGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAaca       >  2:2475853/1‑150 (MQ=255)
                                                                                                                                                 gggTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACg     >  1:1295889/1‑150 (MQ=255)
                                                                                                                                                 gggTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACg     <  1:3907593/150‑1 (MQ=255)
                                                                                                                                                  ggTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGt    >  2:3516374/1‑150 (MQ=255)
                                                                                                                                                   gTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGCAGTTGTGAATCACCCAGCAACACGtt   >  2:1625738/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTG  >  AP012306/2714407‑2714704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑