Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC23.6% 327.4 / 17.2 123L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (80/14);  new base C (0/29);  total (80/43)
Fisher's exact test for biased strand distribution p-value = 6.20e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 183 total aligned reads displayed.

TATTCCCCCTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCGGGGCCGTTGCTGCTTTCTATG  >  AP012306/3182714‑3183009
                                                                                                                                                          |                                                                                                                                                       
tattCCCCCTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGGTTT‑ACCCTTGCATGATGTTTGGCGCAACGCTGGTGCTTCCCTGGTGGGGGCGGGGCC‑GT‑TG‑CC‑TTTATTT‑Tcc                                                                                                                                                       >  2:2279736/1‑150 (MQ=255)
tattCCCCCTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑Acc                                                                                                                                                       <  1:2760252/150‑1 (MQ=255)
 attCCCCCTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑Accc                                                                                                                                                      <  2:4003056/150‑1 (MQ=255)
   tCCCCCTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCg                                                                                                                                                    <  2:1732199/150‑1 (MQ=255)
   tCCCCCTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCAATT‑CCCCCTGCTTGATGATGGGGGCCAAGCGGATTCTTATCTGTTGGGGGGCGGCCACAT‑GC‑CC‑TTATTTT‑Acccc                                                                                                                                                     >  2:2068635/1‑150 (MQ=255)
      cccTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCa                                                                                                                                                 <  1:1263963/150‑1 (MQ=255)
       ccTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTT‑TCCCCGGCATGATGGTGGGGGCAACGCTGATGCTTACCTGGGGGGGGGGGGCCA‑GG‑CG‑CC‑CTTATTT‑ATCCCGcccc                                                                                                                                                >  2:1384868/1‑148 (MQ=255)
       ccTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTT‑CCCCCGGCATGATGGTGGGCGGAACGCTGATGCTTACCTGGTGGTGGGAGGCCC‑GT‑CG‑CC‑CTTATTT‑ATcccccccg                                                                                                                                                >  2:1417008/1‑148 (MQ=255)
       ccTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTT‑CCCCCGGCATGATGATGGGCGCGACGCTGATGCTTACCTCGGGGTGGGGGGGCA‑GC‑CG‑CC‑CTAAATT‑TCcccccccc                                                                                                                                                >  2:2600557/1‑148 (MQ=255)
              ttCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑TCCCCCGCAACATTGTTGGCCGAACGCCGAAGTTTTCCTGGTGGGGGGCGGCCC‑CG‑AA‑CC‑GTAATTT‑CTCCCGCCAACcaaaaa                                                                                                                                         >  2:916108/1‑149 (MQ=255)
               tCCTTTTATTA‑CTTCGGGCTTTCCCAGGGTTTTACCAATAAGTAAGTTTTTTATGGGGGGCATTG‑CCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTCCCGGGGGGTGGCAGGCCA‑GT‑CG‑CT‑TTAATTT‑CCCCCGCCAGCAAAAAGc                                                                                                                                        <  2:1014119/150‑1 (MQ=255)
                   tttATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCAATT‑CCCCTTGGATGAGGATGGGCGCAACGCTGATGCTTTCCTGGTGGTGGGAGGGCA‑GT‑TG‑CC‑CTTATTT‑TTCCCCCCCCCAAAAAGCAACg                                                                                                                                    >  2:2468402/1‑150 (MQ=255)
                      attatCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CT‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATg                                                                                                                                 <  1:3439558/150‑1 (MQ=255)
                       ttatCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCTTTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGc                                                                                                                                <  2:3268864/150‑1 (MQ=255)
                            ttC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTT‑CCCCCCGCCTGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGGCC‑GG‑CG‑CC‑CTAATTT‑ACCCCCCCCGCCAAAAGCAAAGATGCAggga                                                                                                                           >  2:635864/1‑148 (MQ=255)
                             tC‑GGCGTTCCCAGCGGATTATCCACCAGTAAGCTGTTTAGGGCGGGCATTG‑CCCCTGGCATGATGAGGGGCGCACCGCTGATGCTTACCGGGTGGGGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGaaa                                                                                                                          <  2:2308666/150‑1 (MQ=255)
                                ggCTTTCCCAGCGGATTATCCATCAGAAAGTTTTTTATGGCGGGCTTTG‑CCCCTGGGATGATGTTGGGCGAAACGCTGAGGCTTCCGTGGTGGGGGCGGGCAA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATc                                                                                                                        <  2:336593/150‑1 (MQ=255)
                                    tttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGc                                                                                                                    <  1:3625721/150‑1 (MQ=255)
                                     ttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTT‑CCCCCCGGATGATGATAGGGGCAAAGCTGATTCTTTCCTGGGGGGGGGAGGGCA‑GT‑CGCCC‑TTATTTT‑ACCCC‑CCCGCAAAAAACAAAGATGCCGGGAAACTgggg                                                                                                                   >  2:2958869/1‑148 (MQ=255)
                                         aGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGATTG‑CCCCCGGCATGATGATGGGGGCAACGCCGGTGGTTTCCTGGTGGGGGGGGGGCC‑GG‑CG‑CC‑CTTATTT‑TTCCCCCCCGCAAAAAACAACGAAGCAGGGAATATGGCActct                                                                                                               >  2:3090567/1‑150 (MQ=255)
                                          gCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGATTG‑CCCCCGGCCTGTTGATGGGGGCCACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑CTTATTT‑TCCCCCCCCGCAAAAAACAACGATGCAGGAAAACTTGGACTCtt                                                                                                              >  2:3950571/1‑150 (MQ=255)
                                            ggATTATCCATCAGTAAGCTGTTTATGGCGGGCGTTG‑CCCCCCGCTTGTTTATTGGGGGGACGCTGATGCCTTTCCGGCGGGGGCGGGGCA‑GG‑CG‑CCTTTATTTT‑ACCCCCCGACAAAAAAACAAC‑AAACCGGAAAACTGGGACACtttt                                                                                                            >  2:853854/1‑150 (MQ=255)
                                              aTTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGt                                                                                                          <  1:2888020/150‑1 (MQ=255)
                                                           aaGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGCCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTgg                                                                                             <  1:3060734/150‑1 (MQ=255)
                                                            aGCTTTTTAGGGGGGGCTTTC‑CCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTCCCTGGTGGGGGCAGGCCA‑GT‑CC‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTggg                                                                                            <  2:358096/150‑1 (MQ=255)
                                                            aGCTGTTTATGGCGGGCATTT‑CCCCCCGCCTGATGATGGGGGCAACGCTGATGGTTTCCTGGGGGGGGGGGGGCA‑GT‑CG‑CC‑CTTATTT‑TTCCCCCCCGCCAAAAACAACGAAGCAGGGAAACTGGGACCCTTTTTTTTCCGGGATCTtgg                                                                                            >  2:2519663/1‑150 (MQ=255)
                                                             gCTGTTTATGGCGGGCATTT‑CCCCCCGCCTGATGATGGGCGGCACGCCGATTCTTTCCTTGTGGTGGGAGGCCA‑GTCCC‑CC‑TTATTTT‑ACCCC‑CCCGCAAAAAACAAACCAGCAGGGAAACTGGCGCTCTTTTTTTTCCGGGATCTtggg                                                                                           >  2:2703639/1‑149 (MQ=255)
                                                                    tgggggggCATTG‑CCCGTGGCAGGATGAGGGGCGCAACGTTGAGGCTTTCCGTGTGGGGGCGGGCCA‑GT‑CG‑CT‑TTATTTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGttt                                                                                    <  2:1776834/145‑1 (MQ=255)
                                                                     gggCGGGGCTTGCCCCCGGGAATGATGAGGGGCCAAACGCTGATCCTTACCTGGGGGGGGCGGGCCG‑GT‑CG‑‑C‑TTAATTTCCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGttt                                                                                    <  2:3174791/149‑1 (MQ=255)
                                                                       gCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                                 <  1:3507932/150‑1 (MQ=255)
                                                                        cGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                >  2:2549934/1‑150 (MQ=255)
                                                                        cGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                >  2:3183844/1‑150 (MQ=255)
                                                                        cGGGCATTC‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CT‑TTATTTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                <  2:3787806/150‑1 (MQ=255)
                                                                          ggCATTG‑CCGCTGGCATGGTGTTGGGCGCAAGGGTTATGCTTCCTGGGTGCTGGAGGGCGT‑GC‑CG‑CT‑TTAATTG‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                              <  2:1214116/150‑1 (MQ=255)
                                                                          ggCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                              >  2:820983/1‑150 (MQ=255)
                                                                            cATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                            >  1:3380013/1‑150 (MQ=255)
                                                                             aTTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                           >  1:2888137/1‑150 (MQ=255)
                                                                             aTTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                           >  2:131010/1‑150 (MQ=255)
                                                                             aTTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                           >  1:1960747/1‑150 (MQ=255)
                                                                              ttG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtca                                                                          >  1:1359348/1‑150 (MQ=255)
                                                                                g‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                        >  1:557856/1‑150 (MQ=255)
                                                                                g‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                        >  1:4026772/1‑150 (MQ=255)
                                                                                  ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                       >  2:2176840/1‑150 (MQ=255)
                                                                                  ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                       >  2:57753/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                      >  2:369231/1‑150 (MQ=255)
                                                                                    ccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                     <  1:3813809/150‑1 (MQ=255)
                                                                                    ccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                     >  1:1085602/1‑150 (MQ=255)
                                                                                    ccTGGCATGATGAGGGGCGCACCGTTGATGCTTCCCTGGGGGTGGCGGGCCA‑GT‑CG‑CT‑TTATTTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                     <  2:3739397/150‑1 (MQ=255)
                                                                                      gggCATGATGATGGGCGCACCGCTGATGCTTCCCTGGTGGTGGCGGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                   <  2:1653055/149‑1 (MQ=255)
                                                                                       ggCATGATGGGGGGCGCCACGTTGATGCTTCCCTGGTGGTGGCAGGCCG‑GC‑CG‑CC‑TTATTTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                  <  2:782597/150‑1 (MQ=255)
                                                                                       ggCATGATGAGGGGCGCAACGCTGAGGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                  <  1:3569008/150‑1 (MQ=255)
                                                                                         cATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTgg                                                                >  2:4236586/1‑150 (MQ=255)
                                                                                          atgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGc                                                               >  1:1059754/1‑150 (MQ=255)
                                                                                          atgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGc                                                               >  1:1169515/1‑150 (MQ=255)
                                                                                          atgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGc                                                               >  1:1476780/1‑150 (MQ=255)
                                                                                           tgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                              >  2:1253356/1‑150 (MQ=255)
                                                                                            gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                             >  2:133758/1‑150 (MQ=255)
                                                                                            gatgaggGGCGCAACGCTGATGCTTCCCGGGTGGTGGCGGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                             <  2:2688244/150‑1 (MQ=255)
                                                                                                atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTATTTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                                         <  1:2291164/150‑1 (MQ=255)
                                                                                                 tgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgttt                                                        >  2:3921026/1‑149 (MQ=255)
                                                                                                     cgcAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTc                                                    >  1:2600830/1‑150 (MQ=255)
                                                                                                        aaCGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTcccg                                                 >  2:3536379/1‑148 (MQ=255)
                                                                                                           gCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCt                                              <  1:2298357/150‑1 (MQ=255)
                                                                                                           gCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCt                                              >  2:212343/1‑150 (MQ=255)
                                                                                                           gCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGCGTTTCGCTCCGGCCt                                              >  1:4207621/1‑150 (MQ=255)
                                                                                                             tGATGCTTCCCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑t                                           <  1:3198874/150‑1 (MQ=255)
                                                                                                             tGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCGGCCTG‑t                                           >  1:1547572/1‑150 (MQ=255)
                                                                                                                tGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCGGcccgcttt                                         >  2:2028228/1‑144 (MQ=255)
                                                                                                                tGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGCCCG‑TTTa                                        >  1:2424687/1‑150 (MQ=255)
                                                                                                                agCTTCCCGGGGGGGGGCAGGCCG‑GT‑CG‑CT‑TTAATTA‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTa                                        <  2:3132225/149‑1 (MQ=255)
                                                                                                                 gCTTACCTAGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTAc                                       <  1:649610/150‑1 (MQ=255)
                                                                                                                  cTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGCCTG‑TTTTcc                                      >  1:3322155/1‑150 (MQ=255)
                                                                                                                       cTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCTCTCCGGCCCG‑GTTATCCCC‑A‑c                               >  1:1331529/1‑150 (MQ=255)
                                                                                                                        tggtggtggCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCCG‑TTTTCCCCC‑A‑cc                              >  2:2410406/1‑150 (MQ=255)
                                                                                                                          gtggtggCAGGCCA‑GT‑CG‑CT‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGa                            <  1:2226204/150‑1 (MQ=255)
                                                                                                                            ggtggCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCTG‑TTTTCCCCC‑A‑ccccaa                          >  1:2483431/1‑146 (MQ=255)
                                                                                                                             gtggCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGCCCG‑TTTTCCCCC‑A‑CCCAAGc                         >  1:2056601/1‑150 (MQ=255)
                                                                                                                               ggCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCTCTCCGGCCTG‑TTTATCCCT‑A‑CCgcaaccc                       >  2:2432382/1‑144 (MQ=255)
                                                                                                                               ggCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTGCGGCCTG‑TTTTCCCCC‑A‑CCGGAGCCg                       >  2:18895/1‑150 (MQ=255)
                                                                                                                                gCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCgg                      <  1:1751288/150‑1 (MQ=255)
                                                                                                                                  aGGCCA‑GT‑CG‑CT‑TTAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCgggg                    <  1:1385174/150‑1 (MQ=255)
                                                                                                                                    gCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTTCCGCCCG‑TTTTTCCCT‑ACCCGAACCCGGGGc                   >  2:3902729/1‑150 (MQ=255)
                                                                                                                                     ccA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTTCCCCC‑A‑CCCAAGCCGGGGGCg                 >  1:1190092/1‑150 (MQ=255)
                                                                                                                                      cA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCTACCTG‑GTTATTCCC‑G‑CCCCAGCCgggggccc                >  2:3458469/1‑146 (MQ=255)
                                                                                                                                       a‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCTGCCTC‑TTTATCCCC‑A‑CCCAAGCCCGGGGCggt               >  2:361885/1‑148 (MQ=255)
                                                                                                                                       a‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCTG‑TTTTCCCCTCC‑CCCAACCCGGGGcggc                >  1:1768642/1‑147 (MQ=255)
                                                                                                                                            cG‑CT‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCGGGGCCGTtgct            <  1:928565/150‑1 (MQ=255)
                                                                                                                                             g‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGGCTG‑TTTTCTCCT‑C‑CCCCAACCCGGGGCGGtgctt           >  2:2764365/1‑149 (MQ=255)
                                                                                                                                             g‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCTG‑TTTTTCCCC‑T‑CCCAAGCCCGGGGCGtttgtg           >  2:2103711/1‑146 (MQ=255)
                                                                                                                                               cc‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGGGCCGGGCTGCTTTCCCCTT‑C‑CCCAAACCGGGGCGGtggtcg           >  2:2232032/1‑145 (MQ=255)
                                                                                                                                                c‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCGGCCTG‑TTTACCCCC‑A‑CCCCAGCCGGGGCCGGTgctgct         >  1:3954638/1‑150 (MQ=255)
                                                                                                                                                c‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCCG‑TTTTCCCCT‑C‑CCGAAGCCCCGGGCGTTgctgct         >  1:1794909/1‑150 (MQ=255)
                                                                                                                                                 tttAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCGGGGCCGTTgctgct         <  1:1908428/149‑1 (MQ=255)
                                                                                                                                                  ttAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCGGGGCCGTTGCTGCtt        <  2:4093319/150‑1 (MQ=255)
                                                                                                                                                  ttAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCCG‑TTTTTCCCT‑C‑CCCAAGCCGGGGCCGGTGCTGCtt        >  2:1478076/1‑150 (MQ=255)
                                                                                                                                                   tAATTT‑GCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCGGGGCCGTTGCTGCttt       <  2:1425647/150‑1 (MQ=255)
                                                                                                                                                   tAATTT‑CCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCGGGGCCGTTGCTGCttt       <  1:820983/150‑1 (MQ=255)
                                                                                                                                                      ttt‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCCGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTTTTCCCGCCTG‑TTTTCCCCT‑A‑CCCAAGCCGGGGCCGCTGGTGCtttttt    >  2:2146113/1‑147 (MQ=255)
                                                                                                                                                       tt‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCCG‑TTTTCCCCC‑C‑CCCAAGCCCGGGCCGGTGCTGCttttttt   >  1:2964306/1‑146 (MQ=255)
                                                                                                                                                        t‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCG‑TTTTCCCCC‑C‑CCCAAACCCGGGGCGTTTCTTCCTTCtttt  >  2:3590461/1‑147 (MQ=255)
                                                                                                                                                          |                                                                                                                                                       
TATTCCCCCTTCCATTCCGTTTATTATCTTC‑GGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCA‑GT‑CG‑CC‑TTAATTT‑ACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCT‑A‑CCGAAGCCGGGGCCGTTGCTGCTTTCTATG  >  AP012306/3182714‑3183009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑