Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,324,4330TA21.9% 642.6 / 94.3 251intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (152/44);  new base A (0/55);  total (152/99)
Fisher's exact test for biased strand distribution p-value = 2.20e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 298 total aligned reads displayed.

GGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTC  >  AP012306/3324284‑3324581
                                                                                                                                                     |                                                                                                                                                     
ggTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  2:1854471/150‑1 (MQ=255)
ggTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  2:2422030/150‑1 (MQ=255)
ggTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  1:836420/150‑1 (MQ=255)
gctgatgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  1:3323027/148‑1 (MQ=255)
 gTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaata                                                                                                                                                      >  2:1564313/1‑150 (MQ=255)
    atgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaa                                                                                                                                                   <  1:1980079/150‑1 (MQ=255)
     tgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaa                                                                                                                                                  <  1:3038273/150‑1 (MQ=255)
     tgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaa                                                                                                                                                  <  1:3372993/150‑1 (MQ=255)
     tgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaa                                                                                                                                                  <  1:3835237/150‑1 (MQ=255)
      gaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaaa                                                                                                                                                 >  1:992821/1‑150 (MQ=255)
       taCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑c                                                                                                                                               >  2:263423/2‑150 (MQ=255)
       aaCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑c                                                                                                                                               <  2:3115783/150‑1 (MQ=255)
        aCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑cc                                                                                                                                              >  1:946914/1‑150 (MQ=255)
        aCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑cc                                                                                                                                              <  1:2228813/150‑1 (MQ=255)
         cAAGCCGGAATTTATGGGCGGCGTGATTCAGGACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑ccc                                                                                                                                             <  2:2250803/150‑1 (MQ=255)
         cAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑ccc                                                                                                                                             <  2:1707788/150‑1 (MQ=255)
             ccGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGGGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCa                                                                                                                                         <  2:751130/150‑1 (MQ=255)
               ggAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGc                                                                                                                                       >  2:2185194/1‑150 (MQ=255)
               ggAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGc                                                                                                                                       >  1:4282637/1‑150 (MQ=255)
                       tGGGGGGCGTGATTCAGAACAAAGTTGATCCGCAAACGGGAGAAGTGATTGATCGGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCTTTGGGTGAGTTTATTCAGCGAGTTAAGATCAAAATAAAAAC‑CCCGCCAGCAATcatgc                                                                                                                               <  2:139948/150‑1 (MQ=255)
                         ggcgggGTGATTAAAAACAAAGTTGACCCGCAACCCGGAGAAGTGATTGATCAGGTTACGCGGGCCCCCCTGCCCGGGCAATTGACCACATTTGGTGGTTTTATTCAGGGAGTAAAAATCAAAAAAAAAAA‑CCCGCCAGCAATcatgcat                                                                                                                             <  2:3345869/150‑1 (MQ=255)
                           cggcgTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATgg                                                                                                                           >  2:2491362/1‑150 (MQ=255)
                            ggcgTGATTAAAAAAAAATTTGACCCAAAACCCGGAGAAGTGATTGATCGGGGTACGCGGGCCCACCTGCCGGGGAAATTGACCGCATTTGGTGATTTTATTCAGGGATTAAAGATAAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGc                                                                                                                          <  2:1029466/150‑1 (MQ=255)
                                 gATTAAAAACAAAGTGGACCCGCAAACCGAAGAATTTATTAATCAGGGTCCGCGGGCCCACCTGCCGGGGCAATTGACCCCATTTGGGGGGTTTATTCAGGGGGTAAAGATAAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGtt                                                                                                                     <  2:1243934/150‑1 (MQ=255)
                                  aTTCAGAAAAAAGTTGACCCGCAAACCGGAGAAGTGATTGATCAGGGTCCGCTGGACCCCCTGCCGGGGAAATTGACCGCATTTGGTGATTTTATTCAGGGAGTTAAAATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGttt                                                                                                                    <  2:861939/150‑1 (MQ=255)
                                    tCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGgtttt                                                                                                                  >  2:660106/1‑150 (MQ=255)
                                    tCAGAAAAAAGTTCATCCGAAAACCGAAGAAGTGTTTGATCAGGGTACGTTGACCCCCCTGCCCGGGCAATTGACCGCTTTTGGTGGGTTTATTCAGGGAGTTAAGATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGttttt                                                                                                                  <  2:3964498/150‑1 (MQ=255)
                                      aGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTaa                                                                                                                >  1:2892991/1‑150 (MQ=255)
                                      aGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTaa                                                                                                                >  2:1523041/1‑150 (MQ=255)
                                        aaCAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAAcg                                                                                                              >  2:3761452/1‑150 (MQ=255)
                                           aaaGTTGCCCCGCAAACGGGAGAAGTGATTGATCGGGGTCCGCGGGACCCCCTGACCGGGAAATTGACCGCATTTGGTGATTTTATTCGGCGATTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACgcgc                                                                                                           <  2:4214605/150‑1 (MQ=255)
                                           aaaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACgcgc                                                                                                           >  2:585120/1‑150 (MQ=255)
                                            aaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCt                                                                                                          >  1:2837278/1‑150 (MQ=255)
                                            aaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCt                                                                                                          >  1:1489165/1‑150 (MQ=255)
                                               ttGACCCGAAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACGGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATc                                                                                                       <  1:98523/150‑1 (MQ=255)
                                                 gATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGa                                                                                                     >  2:203254/1‑150 (MQ=255)
                                                  aTCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGAt                                                                                                    >  1:3699950/1‑150 (MQ=255)
                                                       cAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAg                                                                                               >  1:124384/1‑150 (MQ=255)
                                                        aaaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGt                                                                                              <  2:393036/150‑1 (MQ=255)
                                                             ggAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCg                                                                                         >  2:4057063/1‑150 (MQ=255)
                                                               agaAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTc                                                                                       >  2:3691326/1‑150 (MQ=255)
                                                                gaggtaattgatAGGGGTACGCTGGACCTCCTGACGGGTCATTTGACCGATTTTGGTGATTTTATTCAGGGATTTAATTTCTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCg                                                                                      <  2:3634693/144‑1 (MQ=255)
                                                                  aGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGAt                                                                                    >  1:4185197/1‑150 (MQ=255)
                                                                     gattgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCggttaa                                                                                 >  2:3189830/1‑145 (MQ=255)
                                                                     gattgatCAGGGTACGCTGGACCACCTGACCGGCAAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTAAAGATCAAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATaaa                                                                                 <  2:3801257/150‑1 (MQ=255)
                                                                      attgatCGGGGTAGGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAg                                                                                <  1:3457295/150‑1 (MQ=255)
                                                                      attgatCAGGGTACGCTGGACCACCTGACGGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAg                                                                                <  1:2267004/150‑1 (MQ=255)
                                                                      attgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAg                                                                                <  1:3528416/150‑1 (MQ=255)
                                                                        ttatCAGGTTCCCCTGCCCCCCCTGCCCGGGCAATCCCCCCCTTTTGGTGATTTTATCCAGCCATTTAAAATCTAAATAAAACC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGAc                                                                              <  2:2524117/148‑1 (MQ=255)
                                                                         gatCAGGGTACGATGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACa                                                                             <  1:1163979/150‑1 (MQ=255)
                                                                           tCAGGGACCGCTGGACCCCCTGACGGGGCAATTGACCGCATTTGGTGATTTTATTCAGAGATTTAAGATAAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAAt                                                                           <  2:2298274/150‑1 (MQ=255)
                                                                            cAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATc                                                                          >  1:3241473/1‑150 (MQ=255)
                                                                             aGGGTACGCTGGACCACCTGACGGGGAAATTGCCCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCt                                                                         <  1:180322/150‑1 (MQ=255)
                                                                             aGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCt                                                                         >  1:1058100/1‑150 (MQ=255)
                                                                               ggTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTc                                                                       >  1:1130217/1‑150 (MQ=255)
                                                                               ggTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTc                                                                       <  1:1622819/150‑1 (MQ=255)
                                                                                     cTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATa                                                                 >  2:492622/1‑150 (MQ=255)
                                                                                        gACCCCCTGACGGGGAAATTGCCCGTATTTGGTGATTTTATTCAGGGAGTTAAGATCAAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAc                                                              <  2:2883290/150‑1 (MQ=255)
                                                                                        gACCACCTACCGGGGCAATTGACCGTTTTTGGGGATTTTATTCAG‑GGGTTTATTTTTAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAc                                                              <  2:2534221/150‑1 (MQ=255)
                                                                                         accgccTGACCGGGCAATTGCCCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAca                                                             <  1:806901/150‑1 (MQ=255)
                                                                                          cccccggACGGGGCAATTGACCGTTTTTGGTGATTTTATTCAGCGAGTTAAGATCTAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcag                                                            <  2:2084073/144‑1 (MQ=255)
                                                                                          cccccTGACCGGGAAATTGACCGCTTTTGGGGATTTTATCAGGCGAGTAAAGACCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcag                                                            <  2:2592170/147‑1 (MQ=255)
                                                                                          ccaccTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcag                                                            >  2:3835255/1‑150 (MQ=255)
                                                                                           cactctgccGGACAATTGCCCGATTTTGGTTAGTTTATTCAGCGAGATAAGATCCAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagc                                                           <  2:3819663/143‑1 (MQ=255)
                                                                                             ccTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagcag                                                         >  1:3945321/1‑150 (MQ=255)
                                                                                             ccTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagcag                                                         >  1:3004147/1‑150 (MQ=255)
                                                                                                gACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAgcgc                                                      >  1:2610265/1‑150 (MQ=255)
                                                                                                     ggCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACga                                                 >  2:3064984/1‑150 (MQ=255)
                                                                                                        aaTTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatga                                              >  1:2331078/1‑150 (MQ=255)
                                                                                                          ttGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGAtt                                            >  1:184871/1‑150 (MQ=255)
                                                                                                           tGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTa                                           <  1:1771043/150‑1 (MQ=255)
                                                                                                             aCCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACg                                         <  1:2301001/150‑1 (MQ=255)
                                                                                                             aCCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACg                                         >  2:2779670/1‑150 (MQ=255)
                                                                                                                gctttgggTGAGTTTATTCAGGGATTTAAGATAAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCAc                                      <  2:1331748/144‑1 (MQ=255)
                                                                                                                gattttGGTGATTTTATTCAGGGATTAAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCAc                                      <  2:2990040/147‑1 (MQ=255)
                                                                                                                gCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGTCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCAc                                      >  1:970430/1‑150 (MQ=255)
                                                                                                                 cATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACg                                     >  1:2061279/1‑150 (MQ=255)
                                                                                                                  aTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACgg                                    >  2:3487969/1‑150 (MQ=255)
                                                                                                                        tGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAg                              <  1:3262954/150‑1 (MQ=255)
                                                                                                                        tGAGTTTATTCAGCGAGTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAg                              <  2:2837278/150‑1 (MQ=255)
                                                                                                                         gAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGa                             >  1:405642/1‑150 (MQ=255)
                                                                                                                          aGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGGTATTGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAACAGCGCAACGATGATTACGCACGGGCTTAGAt                            >  1:2195220/1‑150 (MQ=255)
                                                                                                                            tttATTCAGCGATTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCa                          <  2:3251336/150‑1 (MQ=255)
                                                                                                                              tATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACg                        >  2:1182202/1‑150 (MQ=255)
                                                                                                                              tATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGCCGATAAAGACAATCTTCAGGATAAACAGCCGCGCAACGATGATTACGCACGGGCTTAGATCACg                        >  2:1910796/1‑150 (MQ=255)
                                                                                                                               aTTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGc                       >  1:2447283/1‑150 (MQ=255)
                                                                                                                               aTTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGGTATCGATTTTAGGGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGc                       >  2:357333/1‑150 (MQ=255)
                                                                                                                                  cAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGa                    >  1:4248512/1‑150 (MQ=255)
                                                                                                                                   aGCGAGTTAAGATCTAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGAc                   <  1:477555/150‑1 (MQ=255)
                                                                                                                                       aGTTAAGATATAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTcc               <  2:2307379/150‑1 (MQ=255)
                                                                                                                                             gATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCCCGGGCTTAGATCACGCAGACGTCCGGTAcc         >  1:4141062/1‑150 (MQ=255)
                                                                                                                                             gATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTAcc         >  1:673536/1‑150 (MQ=255)
                                                                                                                                             gATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTAcc         <  1:240721/150‑1 (MQ=255)
                                                                                                                                              aTCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCg        >  1:2625126/1‑150 (MQ=255)
                                                                                                                                              aTCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCg        >  1:3112292/1‑150 (MQ=255)
                                                                                                                                               tCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGa       >  1:568864/1‑150 (MQ=255)
                                                                                                                                                cTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAt      >  1:2557710/1‑150 (MQ=255)
                                                                                                                                                cTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAt      >  1:3898606/1‑150 (MQ=255)
                                                                                                                                                cTAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAt      <  1:2601986/150‑1 (MQ=255)
                                                                                                                                                  aaataaaAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAttt    <  1:1326829/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTC  >  AP012306/3324284‑3324581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑