Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 2,209,361 A→G H98R (CAC→CGC)  suhB → inositol monophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,209,3610AG99.0% 1212.6 / ‑0.9 302H98R (CAC→CGC) suhBinositol monophosphatase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (194/105);  total (196/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 323 total aligned reads displayed.

GCGGTGATTATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCACTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGC  >  AP012306/2209213‑2209506
                                                                                                                                                     |                                                                                                                                                  
 cGGTGATTATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCt                                                                                                                                                  <  1:4137510/150‑1 (MQ=255)
   gTGATTATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTc                                                                                                                                                <  1:1479197/150‑1 (MQ=255)
      aTTATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTcgcg                                                                                                                                             >  2:3579539/1‑150 (MQ=255)
      aTTATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTcgcg                                                                                                                                             <  2:2732352/150‑1 (MQ=255)
       ttATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCgg                                                                                                                                            <  1:945381/150‑1 (MQ=255)
       ttATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCgg                                                                                                                                            >  1:860494/1‑150 (MQ=255)
          tCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGtat                                                                                                                                         <  1:98925/150‑1 (MQ=255)
           cGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGtatc                                                                                                                                        >  2:1258059/1‑150 (MQ=255)
            gACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGtatct                                                                                                                                       >  2:3720635/1‑150 (MQ=255)
               acGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGtatctatc                                                                                                                                    <  1:1324731/150‑1 (MQ=255)
               acGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGtatctatc                                                                                                                                    >  2:164346/1‑150 (MQ=255)
                cGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCg                                                                                                                                   <  2:2698910/150‑1 (MQ=255)
                cGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCg                                                                                                                                   >  1:366632/1‑150 (MQ=255)
                 gATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGc                                                                                                                                  >  2:1775243/1‑150 (MQ=255)
                  aTTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCt                                                                                                                                 >  1:3221615/1‑150 (MQ=255)
                  aTTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCt                                                                                                                                 >  1:3392001/1‑150 (MQ=255)
                    tCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGt                                                                                                                               >  1:1550233/1‑150 (MQ=255)
                      gTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTc                                                                                                                             <  1:2921972/150‑1 (MQ=255)
                       tAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCg                                                                                                                            <  2:2914335/150‑1 (MQ=255)
                        aaaTCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGt                                                                                                                           >  2:2296126/1‑150 (MQ=255)
                        aaaTCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGt                                                                                                                           <  1:1054714/150‑1 (MQ=255)
                         aaTCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTa                                                                                                                          >  2:2767916/1‑150 (MQ=255)
                         aaTCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTa                                                                                                                          >  2:2517594/1‑150 (MQ=255)
                          atattACCCACAGCGCACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTGCAATGGGTTAT‑CGATCCACTGTATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTTTCTCTCGCTGTTCGTAt                                                                                                                         <  2:3195737/147‑1 (MQ=255)
                           tCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGGTGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATc                                                                                                                        <  1:311898/150‑1 (MQ=255)
                           tCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATc                                                                                                                        <  1:564730/150‑1 (MQ=255)
                               aCCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAg                                                                                                                    >  1:2304359/1‑150 (MQ=255)
                                cccACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAgg                                                                                                                   >  1:2517788/1‑150 (MQ=255)
                                 ccACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑c                                                                                                                 >  1:2060294/1‑150 (MQ=255)
                                 ccACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑c                                                                                                                 >  1:3920952/1‑150 (MQ=255)
                                     aGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCa                                                                                                             >  1:1123091/1‑150 (MQ=255)
                                      gCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAACCGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCAc                                                                                                            <  2:1205585/150‑1 (MQ=255)
                                       cacaCCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCAcc                                                                                                           >  1:2390400/1‑150 (MQ=255)
                                        acacCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCg                                                                                                          <  1:515490/150‑1 (MQ=255)
                                            catcatCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGt                                                                                                      >  1:2337350/1‑150 (MQ=255)
                                             atcatcACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGtt                                                                                                     <  2:4105004/150‑1 (MQ=255)
                                              tcatcaCCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTg                                                                                                    >  1:2731555/1‑150 (MQ=255)
                                               catcaCCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGc                                                                                                   >  2:2646133/1‑150 (MQ=255)
                                                  caCCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCtgt                                                                                                <  2:3165742/150‑1 (MQ=255)
                                                   aCCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCtgtg                                                                                               >  2:3816357/1‑150 (MQ=255)
                                                   aCCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCACTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCtgtg                                                                                               <  2:3891607/150‑1 (MQ=255)
                                                    ccGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTgg                                                                                              <  2:1823106/150‑1 (MQ=255)
                                                      gaagaaAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGta                                                                                            >  2:3102457/1‑150 (MQ=255)
                                                        agaaAGCGATGAACTTGAAGGTACTGATCAGGATCTTCAATTGGTTAG‑CGATCGACTGGATGTCGCCACCAACCTGATCAAACGTCTGCCCAGGTTCGCGGTATCAAGCGCTGCTCGTCTCAAAGA‑CCGCACCGAAGTAGCTGCGGtata                                                                                          <  2:1302053/150‑1 (MQ=255)
                                                         gaaAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATAc                                                                                         <  1:1638816/150‑1 (MQ=255)
                                                           aaGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGa                                                                                       >  1:3650148/1‑150 (MQ=255)
                                                             gCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATc                                                                                     <  2:1496550/150‑1 (MQ=255)
                                                             gCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATc                                                                                     <  1:1774832/150‑1 (MQ=255)
                                                               ggTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCt                                                                                   <  2:2815463/150‑1 (MQ=255)
                                                                gTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTa                                                                                  <  2:4276899/150‑1 (MQ=255)
                                                                 tGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTAt                                                                                 >  2:894098/1‑150 (MQ=255)
                                                                  gAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATg                                                                                <  2:1716574/150‑1 (MQ=255)
                                                                   aaCTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGc                                                                               <  2:3286121/150‑1 (MQ=255)
                                                                    aCTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTTCCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCg                                                                              <  1:3997215/150‑1 (MQ=255)
                                                                     cTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGt                                                                             <  1:100269/150‑1 (MQ=255)
                                                                     cTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGt                                                                             >  1:1609614/1‑150 (MQ=255)
                                                                     cTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGt                                                                             >  1:1675008/1‑150 (MQ=255)
                                                                     cTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGt                                                                             >  2:1583308/1‑150 (MQ=255)
                                                                     cTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGt                                                                             <  2:844895/150‑1 (MQ=255)
                                                                       tGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTaa                                                                           >  2:3538888/1‑150 (MQ=255)
                                                                          aGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGa                                                                        >  1:3274616/1‑150 (MQ=255)
                                                                             tACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACt                                                                     <  1:3576918/150‑1 (MQ=255)
                                                                              aCTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTg                                                                    <  2:4237899/150‑1 (MQ=255)
                                                                              aCTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTg                                                                    >  1:4221503/1‑150 (MQ=255)
                                                                                  aTCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCa                                                                >  1:3371184/1‑150 (MQ=255)
                                                                                    cAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCAcc                                                              >  1:814170/1‑150 (MQ=255)
                                                                                        atcttCACTGGGTTAT‑CGATCCACTGGATGGCACTCCCACCTTTATCAACCGTCTGCCGCGCTTCGCGGTATCTATCCCTGTTCGTATCAAAGC‑CCGCCCCGAAGTTGCTGTGGTATCCGATCCTATGCGTAACGAACTGTTCACCGCCa                                                          <  2:7638/147‑1 (MQ=255)
                                                                                        aTGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCa                                                          >  1:3030798/1‑150 (MQ=255)
                                                                                        aTGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCa                                                          <  2:544544/150‑1 (MQ=255)
                                                                                         tGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCAc                                                         >  2:2125144/1‑150 (MQ=255)
                                                                                          gTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACt                                                        >  1:258965/1‑150 (MQ=255)
                                                                                           ttCAATGGGTTTC‑CGCTCCACTGGATGGCACTCCCACCTTTATCAACCGCCTGCCGCGCTCCGCGGTTTCTCTCGCTGTTCGTATCAAGGG‑CCCCCCCGAAGTTGGGGTGGTATACGTTCCTATGCGTAACGAACTGTCCACCGCCACTc                                                       <  2:3211622/150‑1 (MQ=255)
                                                                                            tCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTcg                                                      >  1:2219572/1‑150 (MQ=255)
                                                                                             taaTGGGTTTTCCGACCCACTGGATGGCACTCCCACCTTTACAAACCGTCTGCCGCCCTTCGCGTTTTCTACCGCTGTTCGTATAAAAGC‑CCCCCCCGAAGTTGCTGGGGTATCCGTTCCTATGCGAAACAAACTGTTCACCGCCACTcg                                                      <  2:2306789/149‑1 (MQ=255)
                                                                                              aaTGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTcgcg                                                    >  2:1032853/1‑150 (MQ=255)
                                                                                              aaTGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTcgcg                                                    >  1:1376753/1‑150 (MQ=255)
                                                                                                   gTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAg                                               >  2:1925703/1‑150 (MQ=255)
                                                                                                   gTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAg                                               >  2:3960607/1‑150 (MQ=255)
                                                                                                          gctCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGca                                         >  2:4000261/3‑150 (MQ=255)
                                                                                                           tcccaggggATGCCACTCCCAATTTTAAAAAACGTCTCCCGCGCTCCGGGGTTTCTCCCGCTGTTCGTA‑CAAAGGCCCCCCCCGAGGTGGCTGGGGTATCCGATCCTATGCGAAACGACCTTTTCACCGCCACTCGCGGTCAGGGCGcac                                        <  2:1069392/143‑1 (MQ=255)
                                                                                                           aTCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGcac                                        >  1:3777399/1‑150 (MQ=255)
                                                                                                              cACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGc                                     >  1:2201510/1‑150 (MQ=255)
                                                                                                                cTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTg                                   >  2:3080463/1‑150 (MQ=255)
                                                                                                                 tGGATGGCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGa                                  >  1:1930882/1‑150 (MQ=255)
                                                                                                                      gccACTACCATCTTAAAAAACCGTCGGCCGCGCTTCGCGGTATCTACCGTTGTTCGTATCAAAGC‑CCGCCCCGAAGTTGCTGGGGTATACGACCCTATGCGAAACGACCTGTCCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGc                             <  2:1390919/148‑1 (MQ=255)
                                                                                                                      gccACTACCAATTTTACCAACCGTCGGCCGCGCTTCGCGGTTTCTATCGCTGTTCGTATCAAGGG‑CCGCCCCGAAGTTGCTGGGGTATACGATCTTATGCGTAACGAACTGTCCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGc                             <  2:3444153/148‑1 (MQ=255)
                                                                                                                       gCACTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCt                            >  1:1017385/1‑150 (MQ=255)
                                                                                                                         aCTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTAc                          >  1:1412824/1‑150 (MQ=255)
                                                                                                                          cTACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTAcc                         >  1:3490647/1‑150 (MQ=255)
                                                                                                                           tACCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCg                        >  2:3631908/1‑150 (MQ=255)
                                                                                                                            aCCAACTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGa                       >  1:3535863/1‑150 (MQ=255)
                                                                                                                             ccAACTTTATCAAACGTCTGCCGCACTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGAc                      >  1:661489/1‑150 (MQ=255)
                                                                                                                                 cTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTgcg                  >  2:637197/1‑150 (MQ=255)
                                                                                                                                 cTTTATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTgcg                  >  2:2408891/1‑150 (MQ=255)
                                                                                                                                   ttATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGcgcg                >  2:2491764/1‑150 (MQ=255)
                                                                                                                                    tATCAAACGTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCgg               >  1:3101250/1‑150 (MQ=255)
                                                                                                                                            gTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCg       >  1:2274115/1‑150 (MQ=255)
                                                                                                                                            gTCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCg       >  2:302291/1‑150 (MQ=255)
                                                                                                                                             tCTGCCGCGCTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGc      >  1:1217037/1‑150 (MQ=255)
                                                                                                                                                 ccGCGCTTCGCGGTATCTACCGCTGTTCTTAAAAAAGG‑CCGCACCGAGGTTGCGGTGGTATACGACCCTAGGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTcgc  <  2:2242538/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGGTGATTATCGACACGATTCGTAAATCTTACCCACAGCACACCATCATCACCGAAGAAAGCGGTGAACTTGAAGGTACTGATCAGGATGTTCAATGGGTTAT‑CGATCCACTGGATGGCACTACCAACTTTATCAAACGTCTGCCGCACTTCGCGGTATCTATCGCTGTTCGTATCAAAGG‑CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGC  >  AP012306/2209213‑2209506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑