BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,352,668	Δ1 bp	coding (226/624 nt)	gidB ←	methyltransferase, glucose‑inhibited cell‑division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,352,667	0	G	.	100.0%	2007.7 / NA	403	coding (227/624 nt)	gidB	methyltransferase, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (128/275); total (128/275)

Only 100 of 415 total aligned reads displayed.

CTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCAC  >  AP012306/3352518‑3352812

      cccTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGt                                                                                                                                            >  1:752249/1‑150 (MQ=255)

        cTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGc                                                                                                                                          >  1:393502/1‑150 (MQ=255)

         tGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGcc                                                                                                                                         <  2:3835430/150‑1 (MQ=255)

          gCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCg                                                                                                                                        <  2:466499/150‑1 (MQ=255)

             ctGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACa                                                                                                                                     >  1:1375739/1‑150 (MQ=255)

              tGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACAt                                                                                                                                    <  2:19917/150‑1 (MQ=255)

               gTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGTACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATc                                                                                                                                   <  1:3312540/150‑1 (MQ=255)

               gTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATc                                                                                                                                   <  2:1230768/150‑1 (MQ=255)

                tACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCg                                                                                                                                  >  2:1458046/1‑150 (MQ=255)

                tACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCg                                                                                                                                  <  2:4023375/150‑1 (MQ=255)

                  cTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGAt                                                                                                                                <  2:3547627/150‑1 (MQ=255)

                  cTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGAt                                                                                                                                <  1:1856264/150‑1 (MQ=255)

                      ttCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAAc                                                                                                                            <  2:3447290/150‑1 (MQ=255)

                       tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAAcc                                                                                                                           >  2:801374/1‑150 (MQ=255)

                        cAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCCACAGAGCGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCg                                                                                                                          >  1:1111239/1‑150 (MQ=255)

                        cAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCg                                                                                                                          >  1:1167475/1‑150 (MQ=255)

                        cAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCg                                                                                                                          >  1:2092009/1‑150 (MQ=255)

                         aaTATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGt                                                                                                                         <  2:2511015/150‑1 (MQ=255)

                                 ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTg                                                                                                                 <  1:4101117/150‑1 (MQ=255)

                                 ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTg                                                                                                                 <  2:613773/150‑1 (MQ=255)

                                 ccAGTTTAAGCTCATGTTCCACCTGACGAAGGAAACGCACGCGTTTACCAATGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTGCACCTTg                                                                                                                 <  2:2870387/150‑1 (MQ=255)

                                   aGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCa                                                                                                               >  1:870183/1‑150 (MQ=255)

                                   aGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCa                                                                                                               >  2:1788133/1‑150 (MQ=255)

                                     tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGa                                                                                                             >  1:3633028/1‑150 (MQ=255)

                                     tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGa                                                                                                             <  2:1340956/150‑1 (MQ=255)

                                     tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGa                                                                                                             >  2:857697/1‑150 (MQ=255)

                                        aaGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATAc                                                                                                          <  1:1730709/150‑1 (MQ=255)

                                           cTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGt                                                                                                       <  2:3577603/150‑1 (MQ=255)

                                           cTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGCGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGt                                                                                                       >  2:2131034/1‑150 (MQ=255)

                                              aTGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGcc                                                                                                    >  1:189074/1‑150 (MQ=255)

                                                 ttGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGccacc                                                                                                 <  2:2279462/150‑1 (MQ=255)

                                                 ttGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGccacc                                                                                                 >  2:549883/1‑150 (MQ=255)

                                                  tGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGccacca                                                                                                <  2:2651890/150‑1 (MQ=255)

                                                    cACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACcaca                                                                                              >  1:1809861/1‑150 (MQ=255)

                                                    cACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACcaca                                                                                              <  1:687105/150‑1 (MQ=255)

                                                    cACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACcaca                                                                                              >  1:290019/1‑150 (MQ=255)

                                                    cACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACcaca                                                                                              >  2:3216150/1‑150 (MQ=255)

                                                       cTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                           >  1:2516715/1‑150 (MQ=255)

                                                          aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTa                                                                                        <  1:934317/150‑1 (MQ=255)

                                                            gAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATc                                                                                      <  2:650006/150‑1 (MQ=255)

                                                             aaGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCg                                                                                     <  1:467903/150‑1 (MQ=255)

                                                                gAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgaga                                                                                  >  2:894405/1‑150 (MQ=255)

                                                                gAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgaga                                                                                  >  2:2740259/1‑150 (MQ=255)

                                                                  aaCGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAat                                                                                <  1:1593364/150‑1 (MQ=255)

                                                                    cgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAatat                                                                              <  2:1048605/150‑1 (MQ=255)

                                                                       acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTACT‑GTCCGGTGCCGAAATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATgga                                                                           <  1:787420/150‑2 (MQ=255)

                                                                         gcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGt                                                                         <  2:1689991/150‑1 (MQ=255)

                                                                           gTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTAc                                                                       <  1:2035569/150‑1 (MQ=255)

                                                                             ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                     <  1:3371263/150‑1 (MQ=255)

                                                                             ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                     >  2:3147270/1‑150 (MQ=255)

                                                                              tACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAg                                                                    <  2:1736514/150‑1 (MQ=255)

                                                                                 cAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                 >  2:417080/1‑150 (MQ=255)

                                                                                    ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtctc                                                              <  2:3898984/150‑1 (MQ=255)

                                                                                      cTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                            <  1:2720527/150‑1 (MQ=255)

                                                                                              cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                    >  1:2394562/1‑150 (MQ=255)

                                                                                              cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                    <  1:1459147/150‑1 (MQ=255)

                                                                                              cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                    <  2:1110500/150‑1 (MQ=255)

                                                                                                    gAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGAcc                                              >  1:618315/1‑150 (MQ=255)

                                                                                                    gAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGAcc                                              <  2:3045392/150‑1 (MQ=255)

                                                                                                     aaaTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCTCCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCg                                             <  2:684110/150‑1 (MQ=255)

                                                                                                     aaaTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCg                                             <  1:3816176/150‑1 (MQ=255)

                                                                                                        tGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAg                                          <  1:3142403/150‑1 (MQ=255)

                                                                                                          ggCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTc                                        <  2:3370277/150‑1 (MQ=255)

                                                                                                          ggCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTc                                        <  2:3723806/150‑1 (MQ=255)

                                                                                                          ggCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTc                                        <  2:2776240/150‑1 (MQ=255)

                                                                                                          ggCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTc                                        <  1:2480511/150‑1 (MQ=255)

                                                                                                            cTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAg                                      <  2:3824834/150‑1 (MQ=255)

                                                                                                              tCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGt                                    <  2:924951/150‑1 (MQ=255)

                                                                                                              tCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGt                                    >  2:20628/1‑150 (MQ=255)

                                                                                                                aGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTg                                  <  2:1394529/150‑1 (MQ=255)

                                                                                                                 ggACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGt                                 <  1:3023902/150‑1 (MQ=255)

                                                                                                                  gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTa                                <  1:3259633/150‑1 (MQ=255)

                                                                                                                  gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTa                                <  2:1903187/150‑1 (MQ=255)

                                                                                                                     gCACGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGc                             <  1:2662505/150‑1 (MQ=255)

                                                                                                                        cGATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCttt                          >  1:2954954/1‑150 (MQ=255)

                                                                                                                         gATAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTg                         <  2:745979/150‑1 (MQ=255)

                                                                                                                          aTAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGt                        >  2:2798531/1‑150 (MQ=255)

                                                                                                                          aTAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGt                        <  1:702059/150‑1 (MQ=255)

                                                                                                                          aTAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGt                        <  1:2802964/150‑1 (MQ=255)

                                                                                                                          aTAGAGAGTGGAATGCCTGGCAGTCCT‑GACCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGt                        <  1:2194355/150‑1 (MQ=255)

                                                                                                                           tAGAGAGTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGtt                       >  1:2832615/1‑150 (MQ=255)

                                                                                                                              agagTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCa                    <  2:3473864/150‑1 (MQ=255)

                                                                                                                              agagTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCa                    <  1:403087/150‑1 (MQ=255)

                                                                                                                               gagTGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAt                   <  2:2091922/150‑1 (MQ=255)

                                                                                                                                  tGGAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTa                <  2:326586/150‑1 (MQ=255)

                                                                                                                                   ggAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt               <  2:3963118/150‑1 (MQ=255)

                                                                                                                                   ggAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt               <  2:2331267/150‑1 (MQ=255)

                                                                                                                                   ggAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt               <  1:1335254/150‑1 (MQ=255)

                                                                                                                                    gAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATg              <  2:1295853/150‑1 (MQ=255)

                                                                                                                                    gAATGCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATg              <  2:2928328/150‑1 (MQ=255)

                                                                                                                                        gCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcagc          <  2:2586430/150‑1 (MQ=255)

                                                                                                                                        gCCTGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcagc          <  2:1629151/150‑1 (MQ=255)

                                                                                                                                           tGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata       <  1:3855017/150‑1 (MQ=255)

                                                                                                                                           tGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata       <  2:361821/150‑1 (MQ=255)

                                                                                                                                           tGGCAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCata       <  1:1031369/150‑1 (MQ=255)

                                                                                                                                              cAGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc    <  1:3484964/150‑1 (MQ=255)

                                                                                                                                               aGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa   <  1:3726560/150‑1 (MQ=255)

                                                                                                                                               aGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa   <  2:2445439/150‑1 (MQ=255)

                                                                                                                                               aGTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa   <  2:2695131/150‑1 (MQ=255)

                                                                                                                                                gTCCT‑GTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCAc  >  1:835045/1‑150 (MQ=255)

CTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCAC  >  AP012306/3352518‑3352812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`