Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306524,2970CT23.6% 354.5 / 43.7 144M1M (ATG→ATA) †citDcitrate lyase, acyl carrier (gamma) subunit
Reads supporting (aligned to +/- strand):  ref base C (29/81);  new base T (34/0);  total (63/81)
Fisher's exact test for biased strand distribution p-value = 6.42e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 171 total aligned reads displayed.

CCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCCAGCAGTTGACGT  >  AP012306/524149‑524446
                                                                                                                                                    |                                                                                                                                                         
 cAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCAt                                                                                                                                                         <  1:36074/150‑1 (MQ=255)
   gAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCAttt                                                                                                                                                       <  1:3688097/150‑1 (MQ=255)
   gAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCAttt                                                                                                                                                       <  2:1313115/150‑1 (MQ=255)
     aTGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCAttttt                                                                                                                                                     <  2:1074137/150‑1 (MQ=255)
      tggtggTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTatttttatttttt                                                                                                                                                    >  1:1417380/1‑149 (MQ=255)
       ggtggtGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCa                                                                                                                                                   <  2:2208165/150‑1 (MQ=255)
       ggtggtGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGACGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCa                                                                                                                                                   <  1:3884254/150‑1 (MQ=255)
          ggtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCt                                                                                                                                                <  2:3363244/150‑1 (MQ=255)
           gtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTg                                                                                                                                               <  1:1579340/150‑1 (MQ=255)
           gtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTCATTTTTTACCTg                                                                                                                                               >  1:217381/1‑150 (MQ=255)
           gtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCGGTTTTTTTTTTTTTTTTTATCTg                                                                                                                                               >  2:1175865/1‑150 (MQ=255)
            tgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGt                                                                                                                                              <  1:880708/150‑1 (MQ=255)
             gCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGtt                                                                                                                                             <  1:735119/150‑1 (MQ=255)
                 aTTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGGTTTTTTTTTTTTTTTACCTGtttttt                                                                                                                                         >  2:4122110/1‑147 (MQ=255)
                  ttGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCa                                                                                                                                        <  1:292785/150‑1 (MQ=255)
                   tGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCAt                                                                                                                                       <  1:3352176/150‑1 (MQ=255)
                     cATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTCTTTTTTTCCCGTTtttccttc                                                                                                                                     >  2:1829018/1‑146 (MQ=255)
                     cATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGCGCCTGCAACGGCGGGCCGGATTATTTTTTCTTTTCAACCGTCTTTCAtac                                                                                                                                     >  2:684549/1‑148 (MQ=255)
                      aTCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCTCTTTTTCATG‑Cg                                                                                                                                   >  2:3740765/1‑150 (MQ=255)
                         gCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGTCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑Cgggg                                                                                                                                <  2:2728379/150‑1 (MQ=255)
                           cAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGt                                                                                                                              <  2:615921/150‑1 (MQ=255)
                           cAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGt                                                                                                                              <  1:1680004/150‑1 (MQ=255)
                            aaaCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTTACCTGTTTTTCATG‑Cggggggg                                                                                                                             >  2:2744079/1‑148 (MQ=255)
                             aaCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGGCGGTTTATTTTTCTTTTTCACCTGTTTCTCCTG‑CGGGGGGCt                                                                                                                            >  2:1031060/1‑150 (MQ=255)
                             aaCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTGTTTTTTCCCCCTGTTCTCCTT‑Cgggggggt                                                                                                                            >  2:2919269/1‑147 (MQ=255)
                              aCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTTTTTTTTACCTCGTTCTCTTGTCGGGGGGCt                                                                                                                            >  2:609779/1‑150 (MQ=255)
                              aCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCtt                                                                                                                           <  1:3524304/150‑1 (MQ=255)
                              aCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTCATTTTTTACCTGTTTCTCCTG‑CGGGGGGCtt                                                                                                                           >  1:3526977/1‑150 (MQ=255)
                               cTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTTACCTGTTTTTCATT‑CGGGGGTCttt                                                                                                                          >  1:3650859/1‑150 (MQ=255)
                               cTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCttt                                                                                                                          <  1:4060354/150‑1 (MQ=255)
                               cTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTTTTTTTTACCTGTTTTTCTTT‑Cgggggggttt                                                                                                                          >  2:1648310/1‑145 (MQ=255)
                                tGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATG‑CGGGGGGCtttt                                                                                                                         >  1:1349189/1‑150 (MQ=255)
                                tGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCTGGTTTTCCTG‑CGGGGGGGtttt                                                                                                                         >  2:3035824/1‑150 (MQ=255)
                                  tttCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGa                                                                                                                       <  1:3975053/150‑1 (MQ=255)
                                  tttCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGa                                                                                                                       <  1:532709/150‑1 (MQ=255)
                                      tcAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAg                                                                                                                   <  1:4070644/150‑1 (MQ=255)
                                        aaCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCt                                                                                                                 <  1:2513887/150‑1 (MQ=255)
                                        aaCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTCTTTTTTTTCCTTTTTTTTCTG‑CGGGGGGCTTTTTACGAACt                                                                                                                 >  2:3047668/1‑150 (MQ=255)
                                         aCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTTACGAGCTg                                                                                                                >  1:1179968/1‑150 (MQ=255)
                                           gctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGcc                                                                                                              <  1:3552287/150‑1 (MQ=255)
                                           gctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGGTTTTTTTTATTTTTTCCCCGTTTCTTCTG‑CGGGGGGGCTTTTACGAGCTGcc                                                                                                              >  2:3817139/1‑150 (MQ=255)
                                            ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGGCTTTTGACGAGCTGCcg                                                                                                             >  1:1290543/1‑150 (MQ=255)
                                              gctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCTCTTTTTCTTT‑TGGGGGGCTTTTTACGGGCGTCcgcg                                                                                                           >  2:2359263/1‑150 (MQ=255)
                                              gctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTCACCTGTTTCTCATG‑CGGGGGGCTTTTGACGAGCTGCcgcg                                                                                                           >  1:3369208/1‑150 (MQ=255)
                                                 aTTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTTGTTTTTTTTTTTTTTTTCCCTGTTTCTTCTG‑CCGGGGGCCTTTTAGGGGGTGCCGCGttc                                                                                                        >  2:470745/1‑148 (MQ=255)
                                                 aTTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTcc                                                                                                        <  2:2835416/150‑1 (MQ=255)
                                                   tGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTGTCGAGCTGCCGCGTCCTg                                                                                                      >  1:95915/1‑150 (MQ=255)
                                                     aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTCTCCTG‑CGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGc                                                                                                    >  1:3254933/1‑150 (MQ=255)
                                                     aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGc                                                                                                    >  1:543099/1‑150 (MQ=255)
                                                     aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGTTTATTTTCTTTTTTTACCTGTTTCTCCTG‑CCGGGGGCTTTTTGCGAGCTGCCGCCTCCTGGc                                                                                                    >  2:1231651/1‑150 (MQ=255)
                                                      tttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTCTTTTCATTTTTTACCCGTTTTTCATG‑CGGGGGGGTTTTGTCGAGCTGCCGCGCCCTcgcg                                                                                                   >  2:427411/1‑150 (MQ=255)
                                                      tttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCg                                                                                                   <  1:2586467/150‑1 (MQ=255)
                                                      tttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCCGTTTTTCCTG‑CGGGGGGGTTTTTTCGAGCCGCCCCGTCCTcgcg                                                                                                   >  2:2071027/1‑150 (MQ=255)
                                                       ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTTTTTTTTTCCCGTTTCTCATT‑CGGGGGGGTTTTTAAGGGCCGCCGCGTCCttgggg                                                                                                  >  2:2561702/1‑145 (MQ=255)
                                                        tGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTCTCATG‑CGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCggg                                                                                                 >  1:1412106/1‑150 (MQ=255)
                                                         gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa                                                                                                <  2:367782/150‑1 (MQ=255)
                                                           aGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTTACCCGTTTTTCATG‑CGGGGGGCTTTTGACGAGCTGCCGCGGCCTGGCgggggg                                                                                              >  2:3734777/1‑147 (MQ=255)
                                                           aGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGt                                                                                              <  1:3859825/150‑1 (MQ=255)
                                                              tCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCt                                                                                           <  1:305634/150‑1 (MQ=255)
                                                                gATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑Ca                                                                                        <  1:1405274/150‑1 (MQ=255)
                                                                 atatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑Caa                                                                                       <  1:1154177/150‑1 (MQ=255)
                                                                   atCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGc                                                                                     <  1:1315304/150‑1 (MQ=255)
                                                                     ccTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑g                                                                                  <  2:1971274/150‑1 (MQ=255)
                                                                         cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTc                                                                              <  1:1042732/150‑1 (MQ=255)
                                                                          gTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCT‑CCAACA‑gggttt                                                                             >  1:1633391/1‑146 (MQ=255)
                                                                              cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCTGTTTTTCATT‑CGGGGGGCTTTTTTCGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAAGCA‑GGttttcaa                                                                          >  2:1476262/1‑145 (MQ=255)
                                                                                 gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAaata                                                                      <  2:300775/150‑1 (MQ=255)
                                                                                   ggggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAaataat                                                                    <  2:917726/150‑1 (MQ=255)
                                                                                          tGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGt                                                             <  1:177981/150‑1 (MQ=255)
                                                                                           gCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTg                                                            <  1:3221746/150‑1 (MQ=255)
                                                                                           gCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTg                                                            <  1:1006684/150‑1 (MQ=255)
                                                                                                        tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCa                                               <  2:298183/150‑1 (MQ=255)
                                                                                                             aGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTTACCTGTTTCTCCTG‑CGGGGGGCTTTTGTCGGGCTGCCGCGGCCTGGCGGGGGGGGTCCAAGAG‑GGTTTTGCAAA‑AAAGCAGGGGGGCTGccggggcccggggc                                          >  2:3851923/1‑150 (MQ=255)
                                                                                                                cTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg                                       <  2:1668795/150‑1 (MQ=255)
                                                                                                                  cAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGAt                                     <  1:3838624/150‑1 (MQ=255)
                                                                                                                     gggTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGc                                  <  2:3909918/150‑1 (MQ=255)
                                                                                                                              cAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑Tc                        <  2:3282371/150‑1 (MQ=255)
                                                                                                                                aCGGCGGGCTGGTTTTTTTTTATTTTTTTCCCGTTTTTCATT‑CGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGT‑CCAGCAGGGTTTTCCAAAAAAT‑CAGCGGGGCTGCCGGGGCCCGCGGGGCGGTAGGCGCGGGA‑TCgg                      >  2:796195/1‑149 (MQ=255)
                                                                                                                                aCGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGt                      <  1:1262442/150‑1 (MQ=255)
                                                                                                                                   gcggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGtttt                   <  1:2898573/150‑1 (MQ=255)
                                                                                                                                    cggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGttttt                  >  1:893972/1‑150 (MQ=255)
                                                                                                                                     gggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTTCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAAGGACCAGCGGCGCGATAGCCGCGTGATTCGttttc                  >  2:2883511/1‑149 (MQ=255)
                                                                                                                                     gggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTc                 >  2:3502505/1‑150 (MQ=255)
                                                                                                                                     gggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTc                 <  1:2055305/150‑1 (MQ=255)
                                                                                                                                      ggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCg                >  2:1140376/1‑150 (MQ=255)
                                                                                                                                      ggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCg                >  2:2285631/1‑150 (MQ=255)
                                                                                                                                        cTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CTGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGcc              <  2:632415/150‑1 (MQ=255)
                                                                                                                                        cTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGcc              <  2:3099033/150‑1 (MQ=255)
                                                                                                                                         tGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCca             <  2:990450/150‑1 (MQ=255)
                                                                                                                                          ggTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCcag            <  2:4034790/150‑1 (MQ=255)
                                                                                                                                          ggTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCcag            <  1:1958965/150‑1 (MQ=255)
                                                                                                                                            tttATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCcagca          <  1:2476414/150‑1 (MQ=255)
                                                                                                                                            tttATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCcagca          >  1:1023814/1‑150 (MQ=255)
                                                                                                                                             ttATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCcagcag         >  1:432758/1‑150 (MQ=255)
                                                                                                                                               aTTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCCAGCAGtt       >  2:3382390/1‑150 (MQ=255)
                                                                                                                                               aTTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCCAGCAGtt       >  1:718645/1‑150 (MQ=255)
                                                                                                                                                ttttCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGG‑TCGTTTTTCGCCAGCAGTTg      >  2:2633862/1‑150 (MQ=255)
                                                                                                                                                    cATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCCAGCAGTTGACGt  >  2:534695/1‑150 (MQ=255)
                                                                                                                                                    cATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCCAGCAGTTGACGt  >  1:2926395/1‑150 (MQ=255)
                                                                                                                                                    cATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCCAGCAGTTGACGt  >  1:2661993/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                         
CCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGA‑TCGTTTTTCGCCAGCAGTTGACGT  >  AP012306/524149‑524446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑