Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,360,0480TG27.2% 473.4 / 11.7 191T113P (ACC→CCC) hycBhydrogenase 3, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base T (46/93);  new base G (52/0);  total (98/93)
Fisher's exact test for biased strand distribution p-value = 9.09e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 250 total aligned reads displayed.

TTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGC  >  AP012306/2359899‑2360197
                                                                                                                                                     |                                                                                                                                                     
  tAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTAt                                                                                                                                                     <  1:2586161/150‑1 (MQ=255)
   aGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGGGCTTTCGGGGGAtt                                                                                                                                                    >  1:2302915/1‑150 (MQ=255)
      cGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGc                                                                                                                                                 <  2:948184/150‑1 (MQ=255)
      cGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGc                                                                                                                                                 >  1:1256878/1‑150 (MQ=255)
       gggCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCCGGGGAATGGCg                                                                                                                                                >  2:2783655/1‑150 (MQ=255)
             aTCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGGTTGcc                                                                                                                                          >  2:1600529/1‑150 (MQ=255)
              tCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCg                                                                                                                                         <  1:3382479/150‑1 (MQ=255)
                cgcacgcaGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGa                                                                                                                                       >  2:2239706/1‑150 (MQ=255)
                    cgcaGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAatat                                                                                                                                   >  2:2437313/1‑150 (MQ=255)
                            ggACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGa                                                                                                                           >  1:2496584/1‑150 (MQ=255)
                            ggACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGa                                                                                                                           >  1:47398/1‑150 (MQ=255)
                            ggACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCggg                                                                                                                           >  2:3643248/1‑149 (MQ=255)
                            ggACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACACGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGa                                                                                                                           >  1:3756279/1‑150 (MQ=255)
                             gACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGAc                                                                                                                          <  2:3796641/150‑1 (MQ=255)
                              aCCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGTATTGGCGTTTGCCGGAGTATCCAGCGGACg                                                                                                                         >  2:2840050/1‑150 (MQ=255)
                                 ttGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCt                                                                                                                      <  2:1160750/150‑1 (MQ=255)
                                  tGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGTGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTg                                                                                                                     <  1:3994989/150‑1 (MQ=255)
                                  tGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTg                                                                                                                     <  1:2960304/150‑1 (MQ=255)
                                  tGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTg                                                                                                                     <  2:2355299/150‑1 (MQ=255)
                                  tGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTTCCGGAATATCCAGCGGACGGCTg                                                                                                                     >  1:3036569/1‑150 (MQ=255)
                                    ttCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGcc                                                                                                                   >  2:3170299/1‑150 (MQ=255)
                                     tcatcaAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCg                                                                                                                  <  1:748559/150‑1 (MQ=255)
                                      catcaAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCgg                                                                                                                 >  1:626381/1‑150 (MQ=255)
                                        tcaAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGaa                                                                                                               >  1:1657901/1‑150 (MQ=255)
                                           aaGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGGCGGCTGCCGGGAAAt                                                                                                            >  2:4022337/1‑150 (MQ=255)
                                             gCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGGATATCCCGCGGACGGCTGCCGGGAAATTc                                                                                                          >  2:863844/1‑150 (MQ=255)
                                              cTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGGGCTTTCGGGGGATTTGCGGTTGCCGGGATATCCCGCGGACGGCTGCCGGAAAATTCa                                                                                                         >  2:1703724/1‑150 (MQ=255)
                                              cTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGGGCCGGGGGCGCTTTCGGGGGGTTGGCGCTTGCCGGGATATCCAGCGGCCGGGTGCCGGGAAAATCa                                                                                                         >  2:1465050/1‑150 (MQ=255)
                                               tACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCCGAAAATTCaa                                                                                                        >  2:3119061/1‑150 (MQ=255)
                                                 cAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTTGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAAtt                                                                                                      >  1:2150004/1‑150 (MQ=255)
                                                 cAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAAtt                                                                                                      >  1:2269238/1‑150 (MQ=255)
                                                   aaGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTgc                                                                                                    >  1:2529252/1‑150 (MQ=255)
                                                    aGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTTGCGTTTGCCGGGATATCCAGCGGACGGCTGCCGGAAAATTCAATTgcg                                                                                                   >  1:2901053/1‑150 (MQ=255)
                                                       tCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGGGCCCGGGGGGCCTTAGGGGGGTTGGCGTTTGCAGGCATAACCACCGGACAGCTGCCGGCAAAATCCATTGCGCCa                                                                                                >  2:1251222/1‑150 (MQ=255)
                                                        cacaTTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCCGCGGACGGCCGCCGGGAAATTCAATTGCGCCaa                                                                                               >  2:523004/1‑150 (MQ=255)
                                                        cacaTTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCaa                                                                                               >  1:1307223/1‑150 (MQ=255)
                                                           aTTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACg                                                                                            <  1:527519/150‑1 (MQ=255)
                                                             ttGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTTGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACggg                                                                                          >  1:396490/1‑150 (MQ=255)
                                                             ttGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACggg                                                                                          >  1:709472/1‑150 (MQ=255)
                                                              tGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGc                                                                                         >  2:2724379/1‑150 (MQ=255)
                                                                aCGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAg                                                                                       <  1:2740651/150‑1 (MQ=255)
                                                                aCGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAg                                                                                       >  1:1767785/1‑150 (MQ=255)
                                                                  ggCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGc                                                                                     <  2:3937440/150‑1 (MQ=255)
                                                                    cGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGa                                                                                   >  1:4119231/1‑150 (MQ=255)
                                                                     gATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGAt                                                                                  <  2:917927/150‑1 (MQ=255)
                                                                     gATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGAt                                                                                  >  1:3159252/1‑150 (MQ=255)
                                                                     gATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGAt                                                                                  >  1:3339169/1‑150 (MQ=255)
                                                                       tCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGc                                                                                <  1:2748937/150‑1 (MQ=255)
                                                                         gcgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCg                                                                              >  2:680659/1‑150 (MQ=255)
                                                                         gcgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGGAAATTCAATTGCGCCAAACGGGCAGGCGATGCCg                                                                              >  2:1262850/1‑150 (MQ=255)
                                                                          cgcgAATACCTGGCACCCAGTCAAGCAATGGGCTGACCCGCGCCGGAGCAGGCGGTGCCGGTGGCGCGTTCGGGGTATTGGCCGTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGc                                                                             <  2:1026114/150‑1 (MQ=255)
                                                                             gAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCCGGGGCGCTTTCGGGGGATTGGCGGTTGCCGGAATATCCAGCGGACGGCCGCCGGAAAATTCAATTGCGCCAAACCGGCAGGCGATGCCGccca                                                                          >  2:1923229/1‑150 (MQ=255)
                                                                                 aCCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCtt                                                                      <  2:1616197/150‑1 (MQ=255)
                                                                                     ggCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAg                                                                  <  1:1561102/150‑1 (MQ=255)
                                                                                     ggCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAg                                                                  <  1:651176/150‑1 (MQ=255)
                                                                                      gCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGc                                                                 <  2:1591271/150‑1 (MQ=255)
                                                                                      gCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGc                                                                 <  1:3001749/150‑1 (MQ=255)
                                                                                       cACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCt                                                                <  2:1324927/150‑1 (MQ=255)
                                                                                         cccAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCCAACGGGCAGGCCATGCCCCACCGCCTGCCGCTTa                                                              >  2:651592/1‑150 (MQ=255)
                                                                                           cAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGGCGGTGGGGCTTTCGGGGGATTGGCGGTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACCGGCAGGGGATGCCGCACAGCGTGCAGCTTGCg                                                            >  2:3351483/1‑150 (MQ=255)
                                                                                           cAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACg                                                            <  1:930332/150‑1 (MQ=255)
                                                                                           cAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGGGCCGGGGGCGCCTTCGGGGGATTGGCGTTTGCCGGGAGATCCAGCGGGCCGCTGCCGGGAAATTCCATTGCGCCAAACCGGCAGGCGATTCCGCGCAGCTTGCAGCCTACg                                                            >  2:1739552/1‑150 (MQ=255)
                                                                                            aGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGc                                                           >  2:2013380/1‑150 (MQ=255)
                                                                                              tCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGGAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGcac                                                         >  1:3576817/1‑150 (MQ=255)
                                                                                               cAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGcaca                                                        <  2:848628/150‑1 (MQ=255)
                                                                                               cAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGGAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGcccc                                                        >  2:459916/1‑147 (MQ=255)
                                                                                                  gCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGc                                                     >  1:2917341/1‑150 (MQ=255)
                                                                                                   cAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGGCGGCTGCCGGGAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCCGCTTACGCACAGGCt                                                    >  2:2067848/1‑150 (MQ=255)
                                                                                                   cAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACCCCCAGGCt                                                    >  2:444524/1‑150 (MQ=255)
                                                                                                     aTGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCttt                                                  <  2:1646943/150‑1 (MQ=255)
                                                                                                      tgtgCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttc                                                 <  1:393103/150‑1 (MQ=255)
                                                                                                       gtgCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttca                                                >  2:2819426/1‑150 (MQ=255)
                                                                                                        tgCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcat                                               <  1:3771370/150‑1 (MQ=255)
                                                                                                           tGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCCCAGCTTGCAGCTTACGCACAGGCTttcattca                                            >  2:256726/1‑150 (MQ=255)
                                                                                                             acacGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAAc                                          <  1:1015064/150‑1 (MQ=255)
                                                                                                                 gcgcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCa                                      <  2:394660/150‑1 (MQ=255)
                                                                                                                 gcgcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGGTTGCCGGGATATCCAGCGGGCGGCTGCCGGGAAAATCAATTGCGCCAAACGGGCAGGCGGTGCCGCCCAGCCTGCAGCCTACGCACAGGCTTTTATTCaaacgca                                      >  2:4099080/1‑145 (MQ=255)
                                                                                                                   gcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACg                                    >  1:3851959/1‑150 (MQ=255)
                                                                                                                   gcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGGAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACg                                    >  2:2951990/1‑150 (MQ=255)
                                                                                                                    ccGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGGAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACgg                                   >  2:3741480/1‑150 (MQ=255)
                                                                                                                     cGGAGCAGGCGGTGGCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGc                                  <  2:2302120/150‑1 (MQ=255)
                                                                                                                     cGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGc                                  >  1:2670141/1‑150 (MQ=255)
                                                                                                                     cGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGc                                  >  2:1367218/1‑150 (MQ=255)
                                                                                                                       gAGCAGGCGGTGCCGGTGGGGCTTTCGGGGTATTGGGGTTTGCCGGAATATCCCACGGGCGGCTGCCGGAAAATTCAATTGGGCCAAACGGGCAGGCGATGCCGCACAGCCTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGccc                                >  2:3851667/1‑150 (MQ=255)
                                                                                                                           aGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATc                            >  2:620166/1‑150 (MQ=255)
                                                                                                                           aGGCGGGGCCGGGGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATc                            >  2:1862195/1‑150 (MQ=255)
                                                                                                                            ggCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCCCGGCCCCACCg                           <  2:1805059/150‑1 (MQ=255)
                                                                                                                              cGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGAc                         >  1:3610787/1‑150 (MQ=255)
                                                                                                                               ggTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGAcg                        <  1:2386911/150‑1 (MQ=255)
                                                                                                                                gTGCCGGGGGCGCTTTCGGGGCATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACCGATTGCAGCTTACGAAAAGGCTTTCATTCAACTGCACGGCCCCATCGAcgc                       >  2:3037192/1‑150 (MQ=255)
                                                                                                                                  gCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCgg                     >  2:2180097/1‑150 (MQ=255)
                                                                                                                                      gTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGa                 <  1:85665/150‑1 (MQ=255)
                                                                                                                                      gTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGa                 <  1:2403794/150‑1 (MQ=255)
                                                                                                                                             tttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGtt          >  2:1901427/1‑150 (MQ=255)
                                                                                                                                             tttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGtt          <  1:1921971/150‑1 (MQ=255)
                                                                                                                                              ttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTa         >  1:2186925/1‑150 (MQ=255)
                                                                                                                                                 ggggTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAAcc      <  1:10830/150‑1 (MQ=255)
                                                                                                                                                 ggggTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAAcc      >  2:3523770/1‑150 (MQ=255)
                                                                                                                                                   ggTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCgg    <  1:2693225/150‑1 (MQ=255)
                                                                                                                                                     tATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc  <  1:2616778/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGC  >  AP012306/2359899‑2360197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑