Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG33.3% 365.8 / 11.6 195K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (87/43);  new base G (65/0);  total (152/43)
Fisher's exact test for biased strand distribution p-value = 1.90e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 271 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819
                                                                                                                                                     |                                                                                                                                                     
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGgtt                                                                                                                                                     >  2:2610047/1‑150 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGgtttt                                                                                                                                                   >  1:1934635/1‑150 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTc                                                                                                                                                  <  1:3247397/150‑1 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTc                                                                                                                                                  <  2:311003/150‑1 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTc                                                                                                                                                  >  2:231911/1‑150 (MQ=255)
        tttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAAc                                                                                                                                               <  2:630525/150‑1 (MQ=255)
         ttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCCGGGTTTTCTACg                                                                                                                                              >  2:2674006/1‑150 (MQ=255)
          tGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTAACGa                                                                                                                                             >  2:1392905/1‑150 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGaa                                                                                                                                            <  2:3272832/150‑1 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAAt                                                                                                                                           <  1:224853/150‑1 (MQ=255)
              aaaGTCGTGGCTTGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTc                                                                                                                                         <  2:2392483/150‑1 (MQ=255)
                   cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATaa                                                                                                                                    <  1:703748/150‑1 (MQ=255)
                   cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTAACGAATTTGATaa                                                                                                                                    >  2:3144070/1‑150 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACCAAATCGATAAt                                                                                                                                   >  2:1746853/1‑150 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAAtt                                                                                                                                  <  2:3700983/150‑1 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAAttt                                                                                                                                 <  2:1053743/150‑1 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTTGCAGGGTTTTCAACGAATTCGATAAtttt                                                                                                                                >  2:1327276/1‑150 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAAtttt                                                                                                                                <  1:2926429/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTTAACGAATTTGATAATTTTAt                                                                                                                              >  2:3033608/1‑150 (MQ=255)
                         tGGAGTTCTCATCGCGATCGGCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATTCTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTAt                                                                                                                              <  2:1342826/150‑1 (MQ=255)
                            aGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGt                                                                                                                           <  2:645191/150‑1 (MQ=255)
                              ttCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTtc                                                                                                                         <  1:1100858/150‑1 (MQ=255)
                               tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTtct                                                                                                                        <  2:2807235/150‑1 (MQ=255)
                                ctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTtctc                                                                                                                       >  2:3317467/1‑150 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCtca                                                                                                                      >  1:3447067/1‑150 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCtca                                                                                                                      <  2:3921924/150‑1 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCtca                                                                                                                      >  1:1203223/1‑150 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCtcat                                                                                                                     >  1:1999086/1‑150 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTTAACGAATTCGATAATTTTTTCGTTCTCATCAAcc                                                                                                                >  2:2871162/1‑150 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAAcc                                                                                                                >  1:772235/1‑150 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAAcc                                                                                                                <  2:338836/150‑1 (MQ=255)
                                           cgcctttttTCAGCGCTTCACACAGCTAGTCGGGGTCAAAGACGTTTATACCCATACTCCCCAGAGATTTGCTCGTATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTCTCAACGAATTCGCTAATTTTTTCGTTCTCATCACCCGCCa                                                                                                            <  2:300949/147‑1 (MQ=255)
                                             tcttctTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATa                                                                                                          >  1:3892476/1‑150 (MQ=255)
                                             tcttctTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATa                                                                                                          >  1:2709582/1‑150 (MQ=255)
                                              cttcttCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATaa                                                                                                         >  2:3973010/1‑150 (MQ=255)
                                                tcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACg                                                                                                       >  1:470396/1‑150 (MQ=255)
                                                    ccAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCaa                                                                                                   >  2:2565272/1‑150 (MQ=255)
                                                     cagcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCcaa                                                                                                  >  2:3849500/1‑150 (MQ=255)
                                                       gcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTTAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATg                                                                                                >  2:143416/1‑150 (MQ=255)
                                                         agTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCCACCGCCATAACGCCCAATGCg                                                                                              >  1:86215/1‑150 (MQ=255)
                                                         agTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCg                                                                                              >  1:963925/1‑150 (MQ=255)
                                                          gTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCgg                                                                                             >  1:3682188/1‑150 (MQ=255)
                                                          gTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCgg                                                                                             >  2:2378766/1‑150 (MQ=255)
                                                           ttCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGa                                                                                            >  1:2006491/1‑150 (MQ=255)
                                                            tCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAg                                                                                           >  1:3086215/1‑150 (MQ=255)
                                                             cATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAgg                                                                                          >  1:2057820/1‑150 (MQ=255)
                                                               tACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGct                                                                                        >  2:1558164/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttc                                                                                      >  1:2434935/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAACCGGTTTTTTAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAAAGCCAAATGCGGAGGcttc                                                                                      >  2:2191769/1‑150 (MQ=255)
                                                                      aggcGGCGACAAAGGCGAAAATCCCCATACCCACCAGATTTTTGCTCGTCCCGTCCGACATGGGCGGCGGTTGAGCGGTTTTTACAACAAATTCAAAATTTTTCTCTTTCTCAAAACCCGCCATAACGCCAAATGCGGAGGCTTCTTCaa                                                                                 <  2:525825/147‑1 (MQ=255)
                                                                         cGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCg                                                                              >  1:106223/1‑150 (MQ=255)
                                                                         cGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCg                                                                              >  1:1573908/1‑150 (MQ=255)
                                                                          ggCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAGTTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGCTTCTTCAATCgg                                                                             >  1:1044193/1‑150 (MQ=255)
                                                                          ggCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCgg                                                                             >  1:3355097/1‑150 (MQ=255)
                                                                                aaaGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTgg                                                                       >  1:3596928/1‑150 (MQ=255)
                                                                                 aaGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGc                                                                      >  1:385247/1‑150 (MQ=255)
                                                                                  aGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCa                                                                     >  1:1336928/1‑150 (MQ=255)
                                                                                  aGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAATTTGATAATTTTATCGTTCTCATCAACCCCCCTAACGCCCAATGCGGAGGCTTCTTCAATCGGTACTGGCa                                                                     >  2:4056225/1‑150 (MQ=255)
                                                                                      gTAGATACCCATCCTCGCCAGAGTTTGGCTCGGATCGTTCGGCATGGGGGGGGGGTTAGCAGTTTTTTCAACAATTTCAATATTTTTTTCGTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACa                                                                 <  2:2112624/150‑1 (MQ=255)
                                                                                      gTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACa                                                                 >  2:2950122/1‑150 (MQ=255)
                                                                                            aCCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAc                                                           >  1:1635321/1‑150 (MQ=255)
                                                                                             cccATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACg                                                          >  1:3375109/1‑150 (MQ=255)
                                                                                              ccATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACgg                                                         >  1:3016606/1‑150 (MQ=255)
                                                                                               cATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGt                                                        >  2:854516/1‑150 (MQ=255)
                                                                                                aTACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTTAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAAgggg                                                       >  2:251065/1‑148 (MQ=255)
                                                                                                 tACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGGAGGGTTTTTAACGAATTCGATAATTTTTTCCTTCTCCTCAACCGCCATCAAGCCAAATGCGGAGGCTTCTTCAATCCGTACTGGCATACAAGCCACGGTGc                                                      >  2:2731376/1‑150 (MQ=255)
                                                                                                  aCTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGGGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCa                                                     <  1:23201/150‑1 (MQ=255)
                                                                                                   cTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCaa                                                    >  1:1822794/1‑150 (MQ=255)
                                                                                                      gCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGt                                                 >  1:3219535/1‑150 (MQ=255)
                                                                                                      gCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGt                                                 >  1:3313421/1‑150 (MQ=255)
                                                                                                       ccAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGGAGGGTTTTTAAAGAAGTGGGTAATTTTATGGGTGTCATCAACCGGCATAAAGGCAAAAGGGGAGGGTTTTTCAAAGGGGACTGGCATACAAGCAAAGGGGCAACggg                                                >  2:3474446/1‑148 (MQ=255)
                                                                                                        cAGAGATTTGCTCGGATCGTTAGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTgc                                               >  2:1187739/1‑150 (MQ=255)
                                                                                                          gagaTTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAAAGAATTCGATAATTTTATCCTTCTCATCAACCGCCCAAACGCCCAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAAAGTgcgc                                             >  2:449081/1‑150 (MQ=255)
                                                                                                           agaTTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAAATCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGcc                                            >  2:1859476/1‑150 (MQ=255)
                                                                                                            gaTTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTAACGAATTCGATAATTTTATCGTTCTCCTCAACCGCCCTAACGCCAAATGGGGAGGGTTCTTTAAAAGGCATTGGCATAAAAACAACGGGGCCACGGGCGCCt                                           >  2:927547/1‑150 (MQ=255)
                                                                                                             ttttGCTCGGTTCGTTCGGCTTGGGGGGGGGTTTAGCAGTTTTTACAACAAATTCTATATTTTTATCGTTCTCATAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCtt                                          <  2:1099286/149‑1 (MQ=255)
                                                                                                              tttGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCttt                                         >  1:3146147/1‑150 (MQ=255)
                                                                                                               ttGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGGATTCGGTAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAAGGTGCGACGTGCGCCcttt                                        >  2:228498/1‑150 (MQ=255)
                                                                                                                tGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAAAGCGGAGGCGTCTTCAATCGGGACTGGCATACAAGCAACGGTGCAACGTGCGCCttttt                                       >  2:2425080/1‑150 (MQ=255)
                                                                                                                 gCTGGGCTCGTGCGGCATTGGGGGGGGTTGCGCAGTTTTTACAACAAATTCGATATTTTTATCGTTCTCATAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTc                                      <  2:213520/150‑1 (MQ=255)
                                                                                                                       aTCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTg                                >  1:3503500/1‑150 (MQ=255)
                                                                                                                             cGGCATTGAGGGGGGGTAAGCAGTTTTTTAAACGATTTCGATATTTTTATCGTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGAt                          <  2:3526034/150‑1 (MQ=255)
                                                                                                                             cGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGGGCAACGTGCGCCTTTTTCGACGTGATCGAt                          >  2:1900106/1‑150 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTTGATAATTTTTTTGTTTTCATCCACCGCCATAACGCCAAATGCGGAGGGTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGCGCGCCTTTTTCGACGTGATCGATa                         >  2:1140499/1‑150 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATa                         >  1:1281424/1‑150 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATa                         >  1:2567876/1‑150 (MQ=255)
                                                                                                                               gCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATaa                        >  1:1380239/1‑150 (MQ=255)
                                                                                                                                ttttGAGGGGGGTTTAGCAGTTTTTTCAACGATTTGGATATTTTTATCGTTCTCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAg                       <  1:3592589/148‑1 (MQ=255)
                                                                                                                                    gggggggggTTAGCGTTTTTTACAAAAAATTCGATATTTTTATCTTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATa                   <  2:3447025/144‑1 (MQ=255)
                                                                                                                                          gggTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGag             >  1:3921077/1‑150 (MQ=255)
                                                                                                                                           ggTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            >  1:2766189/1‑150 (MQ=255)
                                                                                                                                           ggTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            >  1:1403778/1‑150 (MQ=255)
                                                                                                                                            gTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagta           >  1:2269674/1‑150 (MQ=255)
                                                                                                                                              tAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtagt         >  1:793263/1‑150 (MQ=255)
                                                                                                                                               agcggttttttCAACGAATTCATTTTTTTTCCCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTc        <  2:1831978/144‑1 (MQ=255)
                                                                                                                                                 cAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      >  1:542021/1‑150 (MQ=255)
                                                                                                                                                   gtttttcCAACAATTTAGATATTTTTATCGTTCTCACAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgc    <  2:3232766/148‑1 (MQ=255)
                                                                                                                                                   ggTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgc    >  2:200190/1‑150 (MQ=255)
                                                                                                                                                    gTTTTAAAACAAATTCAATTTTTTTATCTTCCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgct   <  2:1246154/150‑1 (MQ=255)
                                                                                                                                                     ttttaCAACGATTTCGATATTTTTCTCTTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgctt  <  1:62996/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑