BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,805,951	A→G	intergenic (+150/‑26)	ecnB → / → sugE	entericidin B membrane lipoprotein/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,805,951	0	A	G	100.0%	1360.2 / NA	332	intergenic (+150/‑26)	ecnB/sugE	entericidin B membrane lipoprotein/multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (124/208); total (124/208)

Only 100 of 338 total aligned reads displayed.

AATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATT  >  AP012306/3805804‑3806096

   aaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGcc                                                                                                                                              <  1:3003749/150‑1 (MQ=255)

   aaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGcc                                                                                                                                              >  1:1666152/1‑150 (MQ=255)

     cGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGcccc                                                                                                                                            >  1:2887984/1‑150 (MQ=255)

      ggTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCa                                                                                                                                           >  1:855522/1‑150 (MQ=255)

        tACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAAt                                                                                                                                         >  2:1717427/1‑150 (MQ=255)

         aCGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAAtt                                                                                                                                        <  2:3030307/150‑1 (MQ=255)

          cGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATtc                                                                                                                                       <  2:344424/150‑1 (MQ=255)

           gACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATtct                                                                                                                                      >  1:973671/1‑150 (MQ=255)

              aGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCg                                                                                                                                   <  2:1864877/150‑1 (MQ=255)

               gCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCgg                                                                                                                                  >  1:2110148/1‑150 (MQ=255)

               gCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCgg                                                                                                                                  <  2:2438436/150‑1 (MQ=255)

                cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGa                                                                                                                                 <  2:1684305/150‑1 (MQ=255)

                cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGa                                                                                                                                 <  2:1452427/150‑1 (MQ=255)

                  gtgtCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGc                                                                                                                               <  2:4096909/150‑1 (MQ=255)

                   tgtCGTGCCGTTTGTTTTCTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGcc                                                                                                                              >  2:1192662/1‑150 (MQ=255)

                     tCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTg                                                                                                                            <  2:2952775/150‑1 (MQ=255)

                     tCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTg                                                                                                                            <  1:325001/150‑1 (MQ=255)

                      cGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGa                                                                                                                           >  2:1118431/1‑150 (MQ=255)

                        tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGata                                                                                                                         <  1:516129/150‑1 (MQ=255)

                         gCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGatat                                                                                                                        >  1:1870597/1‑150 (MQ=255)

                          ccGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATg                                                                                                                       <  1:4140593/150‑1 (MQ=255)

                              ttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCt                                                                                                                   <  1:2183482/150‑1 (MQ=255)

                              ttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCt                                                                                                                   <  2:254889/150‑1 (MQ=255)

                                gtttTTTCTGAGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTgg                                                                                                                 <  1:820570/150‑1 (MQ=255)

                                 ttttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGa                                                                                                                <  2:520926/150‑1 (MQ=255)

                                  tttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGAt                                                                                                               <  2:133879/150‑1 (MQ=255)

                                     ttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTAt                                                                                                            <  1:2865495/150‑1 (MQ=255)

                                      tCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATc                                                                                                           >  1:3546644/1‑150 (MQ=255)

                                       cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCt                                                                                                          <  2:2336439/150‑1 (MQ=255)

                                        tGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCtt                                                                                                         <  1:3690131/150‑1 (MQ=255)

                                         gCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTa                                                                                                        <  1:2543609/150‑1 (MQ=255)

                                         gCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTa                                                                                                        >  1:2248623/1‑150 (MQ=255)

                                          cGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAg                                                                                                       <  2:3337005/150‑1 (MQ=255)

                                           gATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGt                                                                                                      >  2:3512557/1‑150 (MQ=255)

                                           caTAGTCACAAATGTAAGAGTAGAAGCTCCTGTGCCTCGTCGCAAAATATCTGTTCCGCCTGCAGCTTTGCAGGAAGACCTGCAAACGCCTCTTGTCCCCGGGGACGGCCCCAATTCTGAGGAGCATGAAATGGCCCAGATTATCATAGt                                                                                                      <  2:1254209/149‑1 (MQ=255)

                                            aTAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGtt                                                                                                     <  2:1407954/150‑1 (MQ=255)

                                             tAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTa                                                                                                    <  2:1922598/150‑1 (MQ=255)

                                               gTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTAtt                                                                                                  <  2:1842149/150‑1 (MQ=255)

                                               gTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTAtt                                                                                                  <  2:3092345/150‑1 (MQ=255)

                                                tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTg                                                                                                 >  1:1975872/1‑150 (MQ=255)

                                                tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTg                                                                                                 >  2:3650490/1‑150 (MQ=255)

                                                  acaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCt                                                                                               <  1:3020815/150‑1 (MQ=255)

                                                    aaaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTgg                                                                                             >  1:3489886/1‑150 (MQ=255)

                                                     aaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGt                                                                                            <  2:1506875/150‑1 (MQ=255)

                                                     aaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCATCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGt                                                                                            >  2:2889480/1‑150 (MQ=255)

                                                        gTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTGTCTTAGTTATTGCTGGTctg                                                                                         >  1:389725/1‑150 (MQ=255)

                                                         tAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTctgc                                                                                        <  2:939567/150‑1 (MQ=255)

                                                               ttGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAg                                                                                  >  1:3569183/1‑150 (MQ=255)

                                                                tGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGt                                                                                 <  1:153476/150‑1 (MQ=255)

                                                                 gAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTg                                                                                <  2:725715/150‑1 (MQ=255)

                                                                    aTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTa                                                                             >  2:3367032/1‑150 (MQ=255)

                                                                    aTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTa                                                                             <  2:2675370/150‑1 (MQ=255)

                                                                     ttCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTAt                                                                            <  1:1815208/150‑1 (MQ=255)

                                                                      tCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATg                                                                           >  2:1059529/1‑150 (MQ=255)

                                                                       ccccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                                                          >  2:1769585/1‑150 (MQ=255)

                                                                        cccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATggg                                                                         <  2:2236462/150‑1 (MQ=255)

                                                                          cTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGcc                                                                       >  2:2026482/1‑150 (MQ=255)

                                                                           tGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCg                                                                      <  1:1005446/150‑1 (MQ=255)

                                                                             ccACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGtt                                                                    <  1:2466532/150‑1 (MQ=255)

                                                                                 cTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGcc                                                                >  1:1335579/1‑150 (MQ=255)

                                                                                  tGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCt                                                               <  1:1185707/150‑1 (MQ=255)

                                                                                  tGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCt                                                               <  2:890289/150‑1 (MQ=255)

                                                                                     cAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaa                                                            >  2:83367/1‑150 (MQ=255)

                                                                                     cAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaa                                                            <  1:743247/150‑1 (MQ=255)

                                                                                       aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAat                                                          >  2:2908029/1‑150 (MQ=255)

                                                                                         atatCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAatat                                                        <  2:3299445/150‑1 (MQ=255)

                                                                                          tatCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAAtata                                                       <  2:3698472/150‑1 (MQ=255)

                                                                                           atCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAc                                                      <  1:1910998/150‑1 (MQ=255)

                                                                                                 tCAACCATCAGCTTTGCAGGACGACCCGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACgg                                                <  1:2768511/150‑1 (MQ=255)

                                                                                                   aaCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGTTGGAGTTGGTATGGGCCGTTGGCCTGAAATTTACCCACGCCt                                              >  1:2634782/1‑150 (MQ=255)

                                                                                                     ccATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCttt                                            <  2:1316051/150‑1 (MQ=255)

                                                                                                       aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAg                                          <  2:3962962/150‑1 (MQ=255)

                                                                                                        tCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGt                                         <  1:174323/150‑1 (MQ=255)

                                                                                                        tCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGt                                         >  2:3571740/1‑150 (MQ=255)

                                                                                                        tCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGt                                         <  2:1576398/150‑1 (MQ=255)

                                                                                                         cAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTc                                        <  1:4081187/150‑1 (MQ=255)

                                                                                                               tGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCATTTGa                                  >  2:2373508/1‑150 (MQ=255)

                                                                                                                gCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGAc                                 <  2:642400/150‑1 (MQ=255)

                                                                                                                gCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGAc                                 <  2:2485449/150‑1 (MQ=255)

                                                                                                                 cAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACg                                >  1:2310695/1‑150 (MQ=255)

                                                                                                                 cAGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACg                                >  1:1208941/1‑150 (MQ=255)

                                                                                                                  aGGACGACCTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGc                               >  2:2091164/1‑150 (MQ=255)

                                                                                                                         ccTGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAgtgt                        <  2:1552696/150‑1 (MQ=255)

                                                                                                                           tGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGtta                      <  2:41021/150‑1 (MQ=255)

                                                                                                                           tGCAAACGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGtta                      <  1:297704/150‑1 (MQ=255)

                                                                                                                              aaaCGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGttatta                   >  2:2289887/1‑150 (MQ=255)

                                                                                                                              aaaCGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGttatta                   <  2:3938641/150‑1 (MQ=255)

                                                                                                                                aCGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACt                 <  1:3061077/150‑1 (MQ=255)

                                                                                                                                 cGCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACtg                >  2:427828/1‑150 (MQ=255)

                                                                                                                                  gCCTCTTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACtgt               >  1:3305030/1‑150 (MQ=255)

                                                                                                                                     tctTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGAc            <  1:1400679/150‑1 (MQ=255)

                                                                                                                                     tctTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGAc            <  1:16899/150‑1 (MQ=255)

                                                                                                                                      ctTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACg           <  2:1094158/150‑1 (MQ=255)

                                                                                                                                      ctTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACg           <  1:556456/150‑1 (MQ=255)

                                                                                                                                      ctTTTCACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGAGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACg           >  2:3845741/1‑150 (MQ=255)

                                                                                                                                           cACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCgat      <  1:2737139/150‑1 (MQ=255)

                                                                                                                                           cACCGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCgat      >  1:212624/1‑150 (MQ=255)

                                                                                                                                              cGGGGGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCgatgat   <  2:757257/150‑1 (MQ=255)

                                                                                                                                               ggggGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGAtt  <  1:1481396/150‑1 (MQ=255)

                                                                                                                                               ggggGCGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGAtt  <  1:381891/150‑1 (MQ=255)

AATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATT  >  AP012306/3805804‑3806096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`