Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,962,255 A→G Y133C (TAT→TGT)  nadR → bifunctional DNA‑binding transcriptional repressor/NMN adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,962,2550AG100.0% 1318.6 / NA 319Y133C (TAT→TGT) nadRbifunctional DNA‑binding transcriptional repressor/NMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (137/182);  total (137/182)

Only 100 of 326 total aligned reads displayed.

TCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCG  >  AP012306/3962108‑3962401
                                                                                                                                                   |                                                                                                                                                  
 ccAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCa                                                                                                                                                 <  1:144917/150‑1 (MQ=255)
 ccAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCa                                                                                                                                                 <  2:331232/150‑1 (MQ=255)
 ccAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCa                                                                                                                                                 <  2:1404296/150‑1 (MQ=255)
      gcgcCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGaaaa                                                                                                                                            >  1:3924469/1‑150 (MQ=255)
       cgcCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGaaaaa                                                                                                                                           <  2:39309/150‑1 (MQ=255)
       cgcCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGaaaaa                                                                                                                                           <  2:3858703/150‑1 (MQ=255)
       cgcCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGaaaaa                                                                                                                                           <  1:823420/150‑1 (MQ=255)
        gcCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAat                                                                                                                                          <  2:2142771/150‑1 (MQ=255)
         ccTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAata                                                                                                                                         >  2:3264357/1‑150 (MQ=255)
            gTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGAAGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTc                                                                                                                                      <  1:2971698/150‑1 (MQ=255)
             tAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCg                                                                                                                                     <  2:974296/150‑1 (MQ=255)
                 cAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCAtt                                                                                                                                 >  1:2271243/1‑150 (MQ=255)
                 cAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCAtt                                                                                                                                 <  1:2481784/150‑1 (MQ=255)
                    gTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCAt                                                                                                                              <  1:3371457/150‑1 (MQ=255)
                    gTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCAt                                                                                                                              <  2:3536702/150‑1 (MQ=255)
                      tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGc                                                                                                                            <  2:2451731/150‑1 (MQ=255)
                      tGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGc                                                                                                                            <  2:3668344/150‑1 (MQ=255)
                       gACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCt                                                                                                                           <  1:1136866/150‑1 (MQ=255)
                       gACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCt                                                                                                                           <  1:1606466/150‑1 (MQ=255)
                        aCGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCtt                                                                                                                          <  2:3000714/150‑1 (MQ=255)
                         cGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCttt                                                                                                                         >  2:2209830/1‑150 (MQ=255)
                         cGAGCTGCATATCATTAAGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCttt                                                                                                                         <  1:2297707/150‑1 (MQ=255)
                           aGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCa                                                                                                                       <  2:1358781/150‑1 (MQ=255)
                            gCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCaa                                                                                                                      <  2:2329421/150‑1 (MQ=255)
                              tGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACg                                                                                                                    >  2:17020/1‑150 (MQ=255)
                               gCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGa                                                                                                                   >  1:1000357/1‑150 (MQ=255)
                               gCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGa                                                                                                                   >  1:2011569/1‑150 (MQ=255)
                               gCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGa                                                                                                                   <  2:3691454/150‑1 (MQ=255)
                                  tatCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAaga                                                                                                                <  2:3587184/150‑1 (MQ=255)
                                   atCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAagag                                                                                                               <  2:2769517/150‑1 (MQ=255)
                                    tCATTATGGGTTTTGACGATGCCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAgg                                                                                                              <  1:3745845/150‑1 (MQ=255)
                                      aTTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGc                                                                                                            <  2:118224/150‑1 (MQ=255)
                                             gTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGc                                                                                                     >  1:4090218/1‑150 (MQ=255)
                                               tttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg                                                                                                   >  2:2058614/1‑150 (MQ=255)
                                                   aCGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATc                                                                                               <  1:3658228/150‑1 (MQ=255)
                                                    cGATATCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATcc                                                                                              <  1:1719344/150‑1 (MQ=255)
                                                    cGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATcc                                                                                              <  1:2590518/150‑1 (MQ=255)
                                                        aCCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCAc                                                                                          <  2:4049486/150‑1 (MQ=255)
                                                            gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCt                                                                                      <  2:3077891/150‑1 (MQ=255)
                                                               aCCGCGCGTTTTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTggg                                                                                   >  1:451990/1‑150 (MQ=255)
                                                               aCCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTggg                                                                                   <  1:2925218/150‑1 (MQ=255)
                                                               aCCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTggg                                                                                   <  2:527002/150‑1 (MQ=255)
                                                                 cgcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAt                                                                                 <  1:3963066/150‑1 (MQ=255)
                                                                 cgcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAt                                                                                 <  2:3493023/150‑1 (MQ=255)
                                                                  gcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtg                                                                                <  1:673427/150‑1 (MQ=255)
                                                                   cgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCGGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATGCGCACGGCTGGGAtgt                                                                               <  2:2021730/150‑1 (MQ=255)
                                                                   cgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgt                                                                               <  1:1163591/150‑1 (MQ=255)
                                                                   cgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgt                                                                               <  1:4035530/150‑1 (MQ=255)
                                                                       ttgttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTgg                                                                           <  1:953153/150‑1 (MQ=255)
                                                                       ttgttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGGTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTgg                                                                           >  2:1451333/1‑150 (MQ=255)
                                                                         gttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                                                                         <  1:2089156/150‑1 (MQ=255)
                                                                            cGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCaa                                                                      <  1:1776258/150‑1 (MQ=255)
                                                                                gACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGc                                                                  <  2:2575413/150‑1 (MQ=255)
                                                                                 aCAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCa                                                                 >  1:3044577/1‑150 (MQ=255)
                                                                                 aCAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCa                                                                 >  1:2950008/1‑150 (MQ=255)
                                                                                  cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCAt                                                                <  2:1546232/150‑1 (MQ=255)
                                                                                   aGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATc                                                               <  1:314604/150‑1 (MQ=255)
                                                                                   aGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAAC‑TTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATTGAGCCGGATTCGCACGGCTGGGATGTGTGGAGCAACGGCATCa                                                              >  2:2726254/1‑150 (MQ=255)
                                                                                    gTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCa                                                              <  2:3819132/150‑1 (MQ=255)
                                                                                     tGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCaa                                                             <  1:685765/150‑1 (MQ=255)
                                                                                          tGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGt                                                        <  2:742339/150‑1 (MQ=255)
                                                                                          tGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGt                                                        <  1:184421/150‑1 (MQ=255)
                                                                                             cGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTa                                                     <  1:2719372/150‑1 (MQ=255)
                                                                                             cGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGCTTa                                                     >  2:1852711/1‑150 (MQ=255)
                                                                                               cagcagCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATg                                                   >  2:1923971/1‑150 (MQ=255)
                                                                                                 gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGc                                                 <  2:876199/150‑1 (MQ=255)
                                                                                                  cagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCt                                                <  1:2216338/150‑1 (MQ=255)
                                                                                                   agcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTg                                               <  2:1135749/150‑1 (MQ=255)
                                                                                                    gcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGa                                              >  2:3166830/1‑150 (MQ=255)
                                                                                                      cGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaa                                            <  2:2063968/150‑1 (MQ=255)
                                                                                                       gACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaaa                                           >  2:3387764/1‑150 (MQ=255)
                                                                                                       gACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaaa                                           <  1:2058007/150‑1 (MQ=255)
                                                                                                        aCCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaaaa                                          >  2:1794089/1‑150 (MQ=255)
                                                                                                           gTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAggg                                       <  1:2490517/150‑1 (MQ=255)
                                                                                                           gTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAggg                                       >  2:913124/1‑150 (MQ=255)
                                                                                                           gTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAggg                                       <  1:718067/150‑1 (MQ=255)
                                                                                                            tGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGa                                      <  2:1263609/150‑1 (MQ=255)
                                                                                                                    cGTCTGCGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCg                              >  1:880631/1‑150 (MQ=255)
                                                                                                                          cGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGgatctg                        >  2:1582831/1‑150 (MQ=255)
                                                                                                                          cGTTGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAAGGATCCAGCCGgatctg                        >  2:4157889/1‑150 (MQ=255)
                                                                                                                            ttGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGgatctgat                      <  2:2501189/150‑1 (MQ=255)
                                                                                                                             tGGTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGgatctgatc                     <  1:1310915/150‑1 (MQ=255)
                                                                                                                               gTTATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTa                   <  1:3285708/150‑1 (MQ=255)
                                                                                                                                ttATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTac                  >  1:212442/1‑150 (MQ=255)
                                                                                                                                ttATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTac                  <  1:3421355/150‑1 (MQ=255)
                                                                                                                                 tATTGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTaca                 <  1:2664400/150‑1 (MQ=255)
                                                                                                                                   ttGCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACAcc               >  2:45015/1‑150 (MQ=255)
                                                                                                                                     gCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTc             <  1:1671839/150‑1 (MQ=255)
                                                                                                                                     gCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTc             <  1:1797042/150‑1 (MQ=255)
                                                                                                                                     gCAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTc             >  2:2241871/1‑150 (MQ=255)
                                                                                                                                      cAAACTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCg            >  1:557192/1‑150 (MQ=255)
                                                                                                                                       aaaCTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCgg           >  1:3241156/1‑150 (MQ=255)
                                                                                                                                        aaCTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGga          >  1:1491593/1‑150 (MQ=255)
                                                                                                                                          cTTTTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGgaag        >  2:1149073/1‑150 (MQ=255)
                                                                                                                                           ttttAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGgaaga       >  1:241907/1‑150 (MQ=255)
                                                                                                                                           ttttAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGgaaga       >  2:3229062/1‑150 (MQ=255)
                                                                                                                                           ttttAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGgaaga       <  1:1948960/150‑1 (MQ=255)
                                                                                                                                            tttAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGgaagaa      >  2:2632785/1‑150 (MQ=255)
                                                                                                                                                aaTGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCg  <  1:483729/150‑1 (MQ=255)
                                                                                                                                                aaTGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGGTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTAAACCTCGGAAGAAGCCg  >  2:2870605/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCG  >  AP012306/3962108‑3962401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑