Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306524,2970CT22.8% 348.6 / 40.3 145M1M (ATG→ATA) †citDcitrate lyase, acyl carrier (gamma) subunit
Reads supporting (aligned to +/- strand):  ref base C (34/78);  new base T (33/0);  total (67/78)
Fisher's exact test for biased strand distribution p-value = 3.03e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 187 total aligned reads displayed.

TCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGT  >  AP012306/524148‑524446
                                                                                                                                                     |                                                                                                                                                        
tCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTc                                                                                                                                                          <  2:3311467/150‑1 (MQ=255)
tCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTc                                                                                                                                                          <  1:1417296/150‑1 (MQ=255)
tCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTc                                                                                                                                                          >  1:2670246/1‑150 (MQ=255)
 ccAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCa                                                                                                                                                         >  1:1065912/1‑150 (MQ=255)
 ccAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCa                                                                                                                                                         >  1:3201547/1‑150 (MQ=255)
 ccAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCa                                                                                                                                                         >  1:3849830/1‑150 (MQ=255)
   aGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCAtt                                                                                                                                                       >  1:140537/1‑150 (MQ=255)
    gAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTAttt                                                                                                                                                      >  1:1596863/1‑150 (MQ=255)
     aaTGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTAtttt                                                                                                                                                     >  2:1729602/1‑150 (MQ=255)
     aaTGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCAtttt                                                                                                                                                     <  1:3168748/150‑1 (MQ=255)
      aTGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTatttttattttt                                                                                                                                                    >  1:1482130/1‑150 (MQ=255)
       tggtggTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGGTGGGTTTTTTTTAtttttt                                                                                                                                                   >  2:3481476/1‑149 (MQ=255)
       tggtggTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTc                                                                                                                                                   <  2:2524983/150‑1 (MQ=255)
       tggtggTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTc                                                                                                                                                   <  2:2366731/150‑1 (MQ=255)
        ggtggtGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTatttttattttttt                                                                                                                                                  >  1:1375936/1‑148 (MQ=255)
        ggtggtGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTatttttatttttta                                                                                                                                                  >  1:2151689/1‑148 (MQ=255)
        ggtggtGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCa                                                                                                                                                  <  2:2823516/150‑1 (MQ=255)
        ggtggtGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGttttttttttttttta                                                                                                                                                  >  2:3925370/1‑148 (MQ=255)
         gtggtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCAc                                                                                                                                                 <  1:895943/150‑1 (MQ=255)
         gtggtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTAc                                                                                                                                                 >  1:2795929/1‑150 (MQ=255)
           ggtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCt                                                                                                                                               <  2:1270830/150‑1 (MQ=255)
             tgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTTTTTTTTTCCTGt                                                                                                                                             >  2:1688626/1‑150 (MQ=255)
              gCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGtt                                                                                                                                            <  2:394210/150‑1 (MQ=255)
                  aTTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTtttc                                                                                                                                        >  2:1037672/1‑150 (MQ=255)
                   ttGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTCTCa                                                                                                                                       >  1:619511/1‑150 (MQ=255)
                     gCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGGAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATg                                                                                                                                     <  2:2285355/150‑1 (MQ=255)
                      cATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑c                                                                                                                                   <  2:2093185/150‑1 (MQ=255)
                        tCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGGGGGGTTGTTTTTATTTTTTTCCAGTTtttctttcag                                                                                                                                  >  2:1543373/1‑144 (MQ=255)
                          gCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑Cgggg                                                                                                                               <  1:1952927/150‑1 (MQ=255)
                          gCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑Cgggg                                                                                                                               <  1:1890561/150‑1 (MQ=255)
                            cAAACTGTTTCTCAACGCTGCTATTGAGTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGt                                                                                                                             <  2:1357100/150‑1 (MQ=255)
                              aaCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTTACCTGTTTCTCATG‑CGGGGGGCt                                                                                                                           >  1:4076689/1‑150 (MQ=255)
                              aaCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGGCt                                                                                                                           >  1:44101/1‑150 (MQ=255)
                               aCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTTTTTTTTTCCTCTTTTTCCTG‑CGGGGGGCtt                                                                                                                          >  2:980379/1‑150 (MQ=255)
                                cTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCttt                                                                                                                         <  2:3728211/150‑1 (MQ=255)
                                 tGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGTTTATTTTTCTTTTTTACCTCTTTCTCCTT‑CGGGGGGCtttt                                                                                                                        >  1:96714/1‑150 (MQ=255)
                                  gTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTCTTTTTTACCTGTTTTTCCTT‑CGGGGGGCttttt                                                                                                                       >  1:1039866/1‑149 (MQ=255)
                                    ttCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTTACCTGTTTTTCATG‑CGGGGGGCTTTTTAc                                                                                                                     >  1:2589053/1‑150 (MQ=255)
                                     tctcAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCA‑TTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGa                                                                                                                   <  1:3871972/150‑1 (MQ=255)
                                       tcAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTTACCCTTTTTTCCTGCCGGGGGGCtttgtgcgg                                                                                                                   >  2:2649194/1‑144 (MQ=255)
                                        cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTTACCCTTTTTTCCTT‑CGGGGGGCTTTTTTCGAGc                                                                                                                 >  2:4223890/1‑150 (MQ=255)
                                        cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTTACGAGc                                                                                                                 >  1:1717538/1‑150 (MQ=255)
                                        cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTGACGAGc                                                                                                                 >  2:3245267/1‑150 (MQ=255)
                                        cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCCGTTTTTCCTG‑CGGGGGGCTTTTTTCGAGc                                                                                                                 >  2:1635889/1‑150 (MQ=255)
                                         aaCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTTCCCGTTTTTCCTG‑CCGGGGGCCTTTTTCGAGCt                                                                                                                >  2:4020878/1‑150 (MQ=255)
                                           cGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGc                                                                                                              <  2:3239306/150‑1 (MQ=255)
                                            gctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTTACGAGCTGcc                                                                                                             >  1:1326349/1‑150 (MQ=255)
                                            gctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGcc                                                                                                             <  1:2501851/150‑1 (MQ=255)
                                             ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTTTTTTTTACCTGTTTCTTTTG‑TGGGGGGCTTTTTTCGAGCTTCcg                                                                                                            >  2:3686452/1‑150 (MQ=255)
                                              tgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTTACGAGCTGCcgc                                                                                                           >  1:1841514/1‑150 (MQ=255)
                                               gctATTGATTTGCAGGTCGATGTCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCAAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCcgcg                                                                                                          <  1:1137026/150‑1 (MQ=255)
                                               gctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGGTTATTTTTATTTTTTACCTGTTTCTCATT‑CGGGGGGCTTTTTACGAGCTGCcgcc                                                                                                          >  1:3990752/1‑149 (MQ=255)
                                                ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTTTTTTTTCACTGCTTCTCTTT‑CGGGGGGGCTTTTTCGAGCTGCcgcgg                                                                                                         >  2:851693/1‑149 (MQ=255)
                                                ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCCGGTTTTTTTTTTTTTTTTTCCTGTTTTTCTTT‑CGGGGGGCTTTTTTCGAGCTGCcgcct                                                                                                         >  2:2061508/1‑148 (MQ=255)
                                                      aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTCTCCTG‑CGGGGGGCTTTTTACCAGCTGCCGCGTCCCGGc                                                                                                   >  1:1364787/1‑150 (MQ=255)
                                                         tGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCggg                                                                                                >  1:3368382/1‑150 (MQ=255)
                                                          gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGATGGGTTTTTTTCTTTTTTTCCCTTTTTCTCGTT‑CGGGGGGGTTTTTGCCGGGTTCCGCGTCctggctggg                                                                                               >  2:3372440/1‑149 (MQ=255)
                                                          gCAGGTCGATAGCCTGCGTTTCGAGTGGGGCGATGCGTATCATCACATCCCCAGGCTCAAGGGTGCCTGGAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGGCGAGCTGCCGCGTCCTGGCGGGa                                                                                               <  2:2756753/150‑1 (MQ=255)
                                                           cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGGTGGGTTATTTTTATTTTTTACCCGTTTTTCATA‑CGGGGGGCTTTTGTCGAGCTGCCGCGTTCTTGgggggg                                                                                              >  2:3761786/1‑148 (MQ=255)
                                                           cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAg                                                                                              <  1:1972510/150‑1 (MQ=255)
                                                            aGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCTTTTTTCACCTGTTTCTCATT‑CGGGGGGCTTTTGTCGAGCTGCCGCGTCCTGGCgggggg                                                                                             >  2:158302/1‑147 (MQ=255)
                                                             ggTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTg                                                                                            <  2:455268/150‑1 (MQ=255)
                                                                cGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑c                                                                                        <  1:524810/150‑1 (MQ=255)
                                                                   tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAg                                                                                     <  1:2735225/150‑1 (MQ=255)
                                                                             aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGc                                                                          <  2:1106862/150‑1 (MQ=255)
                                                                                  gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCCCTTTTTCCTG‑CGGGGGGGTTTTTTCGGGCTGCCGCGGCCCGGCGGGGGGGGT‑CCAGCA‑GGGTTTTCAaata                                                                     >  2:2649942/1‑150 (MQ=255)
                                                                                  gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTACCTGTTTCTCATG‑CGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAACAA‑GGTTTTGCaaaa                                                                      >  1:2996641/1‑149 (MQ=255)
                                                                                     gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATg                                                                  <  2:53068/150‑1 (MQ=255)
                                                                                           tGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGt                                                            <  1:1499832/150‑1 (MQ=255)
                                                                                              gTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTCTTTTTTACCTGTTTCTCATG‑CGGGGGGGTTTTGTCGAGCTGCCGCGTCCTCGCGGGGGGGGT‑CCAGCA‑GGGTTTGTAAAAAATGTAGCGTgga                                                         >  2:346894/1‑148 (MQ=255)
                                                                                               tATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACt                                                        <  1:266440/150‑1 (MQ=255)
                                                                                                 tcatcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGc                                                      <  1:150967/150‑1 (MQ=255)
                                                                                                 tcatcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGc                                                      <  2:2869193/150‑1 (MQ=255)
                                                                                                    tcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAgg                                                   <  1:776296/150‑1 (MQ=255)
                                                                                                        aTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGCCTGCAgggccg                                               <  2:2945257/150‑4 (MQ=255)
                                                                                                              aGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggc                                         <  1:829807/150‑1 (MQ=255)
                                                                                                                   cAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGAt                                    <  1:1572742/150‑1 (MQ=255)
                                                                                                                    aaGGGTGCCTGCAACGGCGGGCTTGTTTTTTTTTCTTTTTCACCCGTTTCTCATG‑CGGGGGGCTTTTTACGAGCTGCCGCGGCCCGGCGGGGGGGGC‑CAAACA‑AGGTTTTCAAACAATTCAGCGTGACTCCCGGGGACCGCGGggcggga                                   >  2:397577/1‑147 (MQ=255)
                                                                                                                       ggTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGcc                                <  1:3817393/150‑1 (MQ=255)
                                                                                                                        gTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCg                               <  1:2222639/150‑1 (MQ=255)
                                                                                                                          gCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTTACCTGTTTCTCATT‑CGGGGGGCTTTTGGCGAGCTGCCGCGTCCTTGGGGGGGGGGT‑CCCACAGGGTTTTCCAAAAAAT‑CAGCGGGACTGGAGGGGCCCGCGGCGGGGGAGCCgcg                             >  2:141696/1‑148 (MQ=255)
                                                                                                                            cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTgag                           <  2:2423763/150‑1 (MQ=255)
                                                                                                                               cAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATc                        <  1:3515847/150‑1 (MQ=255)
                                                                                                                                aaCGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCATGGACCAGCGGCGCGATAGCCGTGAGATCg                       <  2:3809379/150‑1 (MQ=255)
                                                                                                                                   ggcggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGttt                    <  2:215241/150‑1 (MQ=255)
                                                                                                                                   ggcggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGttt                    <  2:3155339/150‑1 (MQ=255)
                                                                                                                                     cggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGttttt                  >  2:34196/1‑150 (MQ=255)
                                                                                                                                      gggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTc                 >  1:3263408/1‑150 (MQ=255)
                                                                                                                                          tGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCca             >  2:3572203/1‑150 (MQ=255)
                                                                                                                                          tGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCca             >  1:2915271/1‑150 (MQ=255)
                                                                                                                                           ggTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCT‑CAAGGA‑GGTTCTGCCAATAATGCCTCGGGGCGGCAACGACCACCCGGGCGATAGGCGGGAGTTAGCTTTTctgcag            >  2:4001197/1‑145 (MQ=255)
                                                                                                                                           ggTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag            >  1:2721449/1‑150 (MQ=255)
                                                                                                                                             tttATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagca          <  1:2851373/150‑1 (MQ=255)
                                                                                                                                               tATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGt        >  1:3023337/1‑150 (MQ=255)
                                                                                                                                                aTTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGtt       <  2:3553326/150‑1 (MQ=255)
                                                                                                                                                 ttttCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGGGAGATCGGTTTTCGCCAGCAGTTg      >  2:652418/1‑150 (MQ=255)
                                                                                                                                                   ttCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTTCAAATAATGCAACGTGGCTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGAc    >  2:2294452/1‑150 (MQ=255)
                                                                                                                                                   ttCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGAc    >  1:2516520/1‑150 (MQ=255)
                                                                                                                                                    tCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACg   >  1:3298886/1‑150 (MQ=255)
                                                                                                                                                     cATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGGGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGt  >  2:247769/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
TCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATG‑CGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCT‑CAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGT  >  AP012306/524148‑524446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑