Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,716,9320TA24.1% 564.1 / 128.7 228intergenic (‑348/‑45)acs/nrfAbifunctional acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base T (156/17);  new base A (0/55);  total (156/72)
Fisher's exact test for biased strand distribution p-value = 4.32e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 245 total aligned reads displayed.

AAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAAC  >  AP012306/3716783‑3717079
                                                                                                                                                      |                                                                                                                                                    
 aaaGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGCTTACTGTCGTGAAAAACAATa                                                                                                                                                     >  1:2820536/1‑150 (MQ=255)
  aaGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATaa                                                                                                                                                    <  1:1265968/150‑1 (MQ=255)
   agagGAAGATACTGACTAACTCTAAAGGGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATaaa                                                                                                                                                   >  1:1156305/1‑150 (MQ=255)
    gagGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATaaaa                                                                                                                                                  <  2:1007878/150‑1 (MQ=255)
    gagGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATaaaa                                                                                                                                                  >  2:441522/1‑150 (MQ=255)
       gAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑cc                                                                                                                                              <  2:513510/150‑1 (MQ=255)
        aaGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCg                                                                                                                                             >  2:2145294/1‑150 (MQ=255)
        aaGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCg                                                                                                                                             >  2:2377862/1‑150 (MQ=255)
        aaGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCg                                                                                                                                             <  1:2800736/150‑1 (MQ=255)
         aGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGc                                                                                                                                            <  1:386070/150‑1 (MQ=255)
           aTACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCa                                                                                                                                          >  2:531229/1‑150 (MQ=255)
           aTACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCa                                                                                                                                          >  2:3175111/1‑150 (MQ=255)
            tACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCAt                                                                                                                                         >  2:2040920/1‑150 (MQ=255)
              cTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTg                                                                                                                                       >  2:1737491/1‑150 (MQ=255)
                gACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAC‑CCGCCATTGca                                                                                                                                     >  1:5664/1‑150 (MQ=255)
                  cTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGcaac                                                                                                                                   >  1:2704794/1‑150 (MQ=255)
                    aaCTCTAAAGTGTTTTTTTACATGCCTTTAAAATTGATTAAAAACAACATTCACAGTTTGGTTATTTTTTACACATAGGGGCAAGAAATGTA‑ATGACAGTGTAGGTGCGTTTACTGTCGAAAAAAACAAAAAAAA‑CCGCCATTGcaacaa                                                                                                                                 <  2:1221328/150‑1 (MQ=255)
                    aaCTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGcaacaa                                                                                                                                 >  1:3745442/1‑150 (MQ=255)
                     aCTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAAt                                                                                                                                >  2:1792993/1‑150 (MQ=255)
                      ctctAAGGTGGTATTTTACATGCATTTAAAATTAATTAAAGACAACATTCAAAGTGTGTTTATTTGTAACAAATGGGGGCGAAAAATGTC‑ATGACGGTGGGGGTGGGGTTCTTGTCGAAAAAAAAAATAAAAA‑CCGCCATTGCAACAATg                                                                                                                               <  2:287736/150‑1 (MQ=255)
                       tctAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTGGGTGCGGTTACTGTCGTGAAAAACAAAAAAAA‑CCGCCATTGCAACAATgg                                                                                                                              <  1:2171371/150‑1 (MQ=255)
                         tAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGgcg                                                                                                                            >  1:1209267/1‑150 (MQ=255)
                              tGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAAtt                                                                                                                       >  2:805079/1‑150 (MQ=255)
                                gTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCg                                                                                                                     >  1:4246280/1‑150 (MQ=255)
                                gTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCg                                                                                                                     >  1:1082298/1‑150 (MQ=255)
                                 tATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCgg                                                                                                                    >  2:4180727/1‑150 (MQ=255)
                                      tACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                               >  2:3163428/1‑150 (MQ=255)
                                      tACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                               >  1:330388/1‑150 (MQ=255)
                                         aTGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGcc                                                                                                            >  1:1789579/1‑150 (MQ=255)
                                            cACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTCGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CTGCCATTGCAACAATGTCGCAATTCGGATGAAGCCCCt                                                                                                         >  1:1941308/1‑150 (MQ=255)
                                              tttAAAATTGATAAAAGAAAAAATTCAAAGTGTGGTTTTTTGTTACAAAGGGGGGCGAGAAATGTC‑ATGAAAGTGTGGGGGGGGTTACTGTGGTAAAAAACAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTAt                                                                                                       <  2:1465043/149‑1 (MQ=255)
                                              cTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTAt                                                                                                       >  1:503341/1‑150 (MQ=255)
                                                 aCAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGAc                                                                                                    >  1:2196607/1‑150 (MQ=255)
                                                  cAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACa                                                                                                   >  2:764329/1‑150 (MQ=255)
                                                  cAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACa                                                                                                   >  1:397171/1‑150 (MQ=255)
                                                  cAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACa                                                                                                   >  2:2299276/1‑150 (MQ=255)
                                                      tGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGa                                                                                               >  1:1679571/1‑150 (MQ=255)
                                                      tGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGa                                                                                               >  2:4200450/1‑150 (MQ=255)
                                                        ataaaaGACAACTTTCACGGTGTGTTTTTTTTTTAAACAAGGGGGCGAGAAAGGCA‑ATGAAAGTGTGGGGGGGTTTACTGTGGAAAAAAAAAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATa                                                                                             <  2:1493728/147‑1 (MQ=255)
                                                         ttAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATaa                                                                                            >  1:2344818/1‑150 (MQ=255)
                                                          tAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATaaa                                                                                           >  2:3683942/1‑150 (MQ=255)
                                                            aaGAAAACATTCACGGTGTGGTTTTTTGTAACAAATGGGGGCGAGAAATGTA‑ATGACAGTGTGGGGGGGGTTACTGTGGTAAAAAAAAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATaaaaa                                                                                         <  2:2571955/150‑1 (MQ=255)
                                                             aGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAAt                                                                                        >  2:1887373/1‑150 (MQ=255)
                                                              gACAACATTCACGGTGTGGTTATTTGTTACACAGGGGGGCGAGCAATGTC‑ATGACAGTGGGGGTGGGGTTACTGTCGTAAAAAAAAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATa                                                                                       <  1:3637556/150‑1 (MQ=255)
                                                               acaacaTTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATaa                                                                                      >  1:3549357/1‑150 (MQ=255)
                                                                caacaTTCACGGTTTGTTTTTTTTTTACGAAGAGGGGCGAAAAATGTC‑AGGCAAGTGTAGGTGGGTTTACGGTCGGAAAAAAAAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATaaa                                                                                     <  2:658775/150‑1 (MQ=255)
                                                                 aacaTTCACAGTGTGGTTATTTGTTACACAAAGGGAAAAGAAATGAC‑ATGACAGTGTAGGGGCGGTTACTGTGGTGAAAAACAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAac                                                                                    <  2:182171/150‑1 (MQ=255)
                                                                  acaTTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacg                                                                                   >  1:2305530/1‑150 (MQ=255)
                                                                   caTTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgc                                                                                  >  1:306562/1‑150 (MQ=255)
                                                                    aTTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgca                                                                                 >  1:1575161/1‑150 (MQ=255)
                                                                    aTTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgca                                                                                 >  1:2592816/1‑150 (MQ=255)
                                                                        cctgtgtgtTTTTTTGTTCCAAAAGGGGCCGAGAAATGTC‑ATGCCGTTGTAGGTGCGTTTCCTGTCGTAAAAAACAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGcc                                                                             <  2:617519/147‑1 (MQ=255)
                                                                        actgtgtgtTTTTTTTTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTGGGTGCGGTTACTGTCGAAAAAAACAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGcc                                                                             <  2:4016737/147‑1 (MQ=255)
                                                                        acaGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGcc                                                                             >  2:1302414/1‑150 (MQ=255)
                                                                            tgtgGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTAt                                                                         >  2:1138339/1‑150 (MQ=255)
                                                                             gtgGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATc                                                                        >  2:3078606/1‑150 (MQ=255)
                                                                              tgGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCt                                                                       >  2:3327738/1‑150 (MQ=255)
                                                                                   aTTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGc                                                                  >  1:3275108/1‑150 (MQ=255)
                                                                                      tGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTa                                                               >  1:1379876/1‑150 (MQ=255)
                                                                                      tGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTa                                                               >  2:3717118/1‑150 (MQ=255)
                                                                                        ttACAAAAAGGGGGGAAAAATGTCAATGATGGTGTGGGTGGGTTTACTGGGGAAAAAAAAAAAAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTAt                                                              <  2:2189042/150‑1 (MQ=255)
                                                                                         tACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTg                                                            >  1:851842/1‑150 (MQ=255)
                                                                                         gacacATGGGGGAGAGAAGTGTA‑ATGAGGGGGTAGGGGTTGTTTGTTTCGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTg                                                            <  1:3525316/149‑1 (MQ=255)
                                                                                            acaggggggCGACAAATGTA‑ATGACGGTGTGGGGGGGGTTATTGTCGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGAtt                                                         <  1:544948/145‑1 (MQ=255)
                                                                                             caTAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTc                                                        >  1:2619276/1‑150 (MQ=255)
                                                                                                 ggggggAGAAATTTC‑AGGACGGGGGGGGTGCGGTTCCTGGCGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCttt                                                    <  1:2935434/150‑1 (MQ=255)
                                                                                                 ggggCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCttt                                                    >  1:3207701/1‑150 (MQ=255)
                                                                                                  ggggGAGAAATGTC‑ATGACGGTGTGGGTGGGGTTCCTGCGGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCtttt                                                   <  1:1903321/150‑1 (MQ=255)
                                                                                                    gCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCtttttt                                                 >  2:191619/1‑150 (MQ=255)
                                                                                                    gCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCtttttt                                                 >  2:1642883/1‑150 (MQ=255)
                                                                                                     cGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttc                                                >  1:2401611/1‑150 (MQ=255)
                                                                                                      gAGCAATTTC‑ATGACGGTGTGGGTGGGTTTATTGTCGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttct                                               <  1:3779037/150‑1 (MQ=255)
                                                                                                       aGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttctt                                              >  2:2843173/1‑150 (MQ=255)
                                                                                                        gCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGTGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttcttt                                             >  1:1113390/1‑150 (MQ=255)
                                                                                                          attGTC‑AGGAAGGTGTGGGGGCGTTTACTGTCGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttcttttt                                           <  1:4041117/148‑1 (MQ=255)
                                                                                                          aaTGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttcttttt                                           >  2:3763823/1‑150 (MQ=255)
                                                                                                           aTGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttctttttc                                          >  2:3469620/1‑150 (MQ=255)
                                                                                                            tGTC‑ATGACGGTGTGGGGGGGTTTCCTGTCGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCa                                         <  1:3879682/150‑1 (MQ=255)
                                                                                                            tGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCa                                         >  1:1707152/1‑150 (MQ=255)
                                                                                                            tGTA‑ATGACAGTGGGGGGGGGGTTCTTGTTGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCa                                         <  2:3932672/150‑1 (MQ=255)
                                                                                                                 aggACGGTGTAGGCGCGGTTACGGTCGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTACTGATTCCTTTTTTCTTTTTCACTTc                                     <  1:706450/148‑1 (MQ=255)
                                                                                                                   gACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTg                                   >  2:2984983/1‑150 (MQ=255)
                                                                                                                    acgttggaGGGGCGTTTCCTGTGGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGt                                  <  2:71399/143‑1 (MQ=255)
                                                                                                                        tgtAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCAc                              >  2:1665022/1‑150 (MQ=255)
                                                                                                                         ggaGGTGCGTTTCCTGTGGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACg                             <  1:616012/148‑1 (MQ=255)
                                                                                                                          tAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGc                            >  1:228182/1‑150 (MQ=255)
                                                                                                                          tAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGc                            >  2:1873320/1‑150 (MQ=255)
                                                                                                                           aGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCt                           >  1:3843755/1‑150 (MQ=255)
                                                                                                                               gCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAAc                       >  2:273548/1‑150 (MQ=255)
                                                                                                                                 ggTTATTGTAGAAAAAAAAAAAAAAAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACaa                     <  2:3570229/150‑1 (MQ=255)
                                                                                                                                 ggTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACaa                     >  2:3952442/1‑150 (MQ=255)
                                                                                                                                  gTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACaaa                    >  2:3421083/1‑150 (MQ=255)
                                                                                                                                   ttACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAAc                   >  2:3100630/1‑150 (MQ=255)
                                                                                                                                     ctcgTCGAGAAAAAAAAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACg                  <  2:2712376/147‑1 (MQ=255)
                                                                                                                                      cTGTCGAAAAAAAAAAAAAAAC‑CCGCCATTGAAACAATGGCGCAATTCGGATGAAGCCCCTATGAAAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGgc                <  2:437238/150‑1 (MQ=255)
                                                                                                                                           gTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTc           >  2:1271469/1‑150 (MQ=255)
                                                                                                                                            aaaaaaaaaaaaaaAC‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTcc          <  1:3222252/148‑1 (MQ=255)
                                                                                                                                             aaaaaaaaaaaaaTT‑CCGCCATTGCAAAAAGGGTGCATTTCGGATGAATCCCATATGAAAAGAATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTccc         <  2:2624209/149‑1 (MQ=255)
                                                                                                                                                    aaTAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAAc  >  1:740910/1‑150 (MQ=255)
                                                                                                                                                    aaTAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAAc  >  2:877107/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTC‑ATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAAC  >  AP012306/3716783‑3717079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑