Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,396,759 T→C L377P (CTG→CCG)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,396,7590TC99.7% 1548.5 / ‑5.2 381L377P (CTG→CCG) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (198/182);  total (198/183)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

Only 100 of 394 total aligned reads displayed.

ACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAA  >  AP012306/3396612‑3396905
                                                                                                                                                   |                                                                                                                                                  
 cGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCa                                                                                                                                                 <  1:2160149/150‑1 (MQ=255)
    aGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGaa                                                                                                                                              <  1:784613/150‑1 (MQ=255)
      ttATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGaaaa                                                                                                                                            >  1:2501829/1‑150 (MQ=255)
      ttATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGaaaa                                                                                                                                            >  1:2932807/1‑150 (MQ=255)
       tATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAAt                                                                                                                                           <  1:3781498/150‑1 (MQ=255)
        aTCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAAtg                                                                                                                                          >  1:2846644/1‑150 (MQ=255)
           tACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTgg                                                                                                                                       >  2:2155494/1‑150 (MQ=255)
            aCGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGCCTCAGGAAGAACCGCAGAAAATGTGGa                                                                                                                                      <  2:65565/150‑1 (MQ=255)
             cGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGAt                                                                                                                                     >  1:1961607/1‑150 (MQ=255)
             cGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGAt                                                                                                                                     <  2:369298/150‑1 (MQ=255)
              gAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATc                                                                                                                                    >  2:1827881/1‑150 (MQ=255)
               aaGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATcc                                                                                                                                   >  2:218812/1‑150 (MQ=255)
                 gagTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTg                                                                                                                                 >  1:1574655/1‑150 (MQ=255)
                 gagTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTg                                                                                                                                 <  1:1368931/150‑1 (MQ=255)
                    tttAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTgcgc                                                                                                                              <  2:3688407/150‑1 (MQ=255)
                      tAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCaa                                                                                                                            >  2:3607247/1‑150 (MQ=255)
                       aaaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCaaa                                                                                                                           >  2:4003271/1‑150 (MQ=255)
                        aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCaaaa                                                                                                                          <  2:3122600/150‑1 (MQ=255)
                         aGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAAt                                                                                                                         <  2:267448/150‑1 (MQ=255)
                           gTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCa                                                                                                                       >  2:739505/1‑150 (MQ=255)
                                ggCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCAc                                                                                                                  <  1:2473356/150‑1 (MQ=255)
                                 gCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCAcc                                                                                                                 >  1:990607/1‑150 (MQ=255)
                                 gCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCAcc                                                                                                                 <  2:2920206/150‑1 (MQ=255)
                                     cATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGAt                                                                                                             >  2:1395090/1‑150 (MQ=255)
                                        ggAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATggg                                                                                                          >  2:3317549/1‑150 (MQ=255)
                                        ggAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATggg                                                                                                          >  2:3207146/1‑150 (MQ=255)
                                            cTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGaa                                                                                                      >  1:3595311/1‑150 (MQ=255)
                                             tGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGaaa                                                                                                     >  1:633488/1‑150 (MQ=255)
                                                 ccTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGa                                                                                                 >  1:2331543/1‑150 (MQ=255)
                                                  ctctctCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGAt                                                                                                <  1:3019354/150‑1 (MQ=255)
                                                  ctctctCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGAt                                                                                                <  2:197121/150‑1 (MQ=255)
                                                  ctctctCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGAt                                                                                                <  1:4151458/150‑1 (MQ=255)
                                                   tctctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATg                                                                                               <  1:496461/150‑1 (MQ=255)
                                                    ctctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGc                                                                                              >  2:1047930/1‑150 (MQ=255)
                                                    ctctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGc                                                                                              <  1:3933447/150‑1 (MQ=255)
                                                     tctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCa                                                                                             <  2:1250211/150‑1 (MQ=255)
                                                       tcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAAt                                                                                           <  1:1269274/150‑1 (MQ=255)
                                                       tcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAAt                                                                                           >  1:925136/1‑150 (MQ=255)
                                                       tcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAAt                                                                                           <  2:2377422/150‑1 (MQ=255)
                                                       tcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAAt                                                                                           <  2:3388650/150‑1 (MQ=255)
                                                        cGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATg                                                                                          <  2:2404783/150‑1 (MQ=255)
                                                         gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATgg                                                                                         >  2:3458014/1‑150 (MQ=255)
                                                           aaGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCGCCCGATGGGCGAAATCGATGCAATGGaa                                                                                       <  1:2773412/150‑1 (MQ=255)
                                                              aTCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTc                                                                                    >  2:3586862/1‑150 (MQ=255)
                                                              aTCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTc                                                                                    <  1:3131254/150‑1 (MQ=255)
                                                               tCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTcc                                                                                   >  2:2774835/1‑150 (MQ=255)
                                                                  cTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCa                                                                                <  1:1445101/150‑1 (MQ=255)
                                                                  cTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCa                                                                                >  2:1470745/1‑150 (MQ=255)
                                                                    gAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCAtt                                                                              >  2:3865162/1‑150 (MQ=255)
                                                                     aaaaaCGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGAGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATta                                                                             >  2:1545243/1‑150 (MQ=255)
                                                                          cgcgTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATaaa                                                                        <  1:3069626/150‑1 (MQ=255)
                                                                            cgTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACt                                                                      <  1:2690789/150‑1 (MQ=255)
                                                                            cgTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACt                                                                      <  1:1155310/150‑1 (MQ=255)
                                                                             gTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTg                                                                     >  1:4122141/1‑150 (MQ=255)
                                                                                 tCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCaa                                                                 <  2:1194751/150‑1 (MQ=255)
                                                                                  cccGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAAt                                                                >  2:1908488/1‑150 (MQ=255)
                                                                                    cGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATga                                                              >  2:1123271/1‑150 (MQ=255)
                                                                                    cGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATga                                                              <  1:670300/150‑1 (MQ=255)
                                                                                    cGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATga                                                              >  1:2092272/1‑150 (MQ=255)
                                                                                      gCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgacc                                                            >  2:1629878/1‑150 (MQ=255)
                                                                                       cTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgacca                                                           >  1:1696481/1‑150 (MQ=255)
                                                                                       cTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgacca                                                           <  1:1428148/150‑1 (MQ=255)
                                                                                       cTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgacca                                                           <  2:286458/150‑1 (MQ=255)
                                                                                          tCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaaga                                                        <  1:2644030/150‑1 (MQ=255)
                                                                                           cGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaagac                                                       >  2:2076602/1‑150 (MQ=255)
                                                                                            gACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaagacc                                                      >  1:1801376/1‑150 (MQ=255)
                                                                                             aCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaagacca                                                     <  1:4229953/150‑1 (MQ=255)
                                                                                              cTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaagaccaa                                                    <  2:3787705/150‑1 (MQ=255)
                                                                                              cTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGAGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaagaccaa                                                    <  2:1076714/150‑1 (MQ=255)
                                                                                               tACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAAt                                                   >  1:334722/1‑150 (MQ=255)
                                                                                               tACAACCGATCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAACAGTGGATCCTACGCAAAATCATACACCAGAAGGGCGAAACCGATGCAATGGAACTCCTCATTAATAAACTGCCAATGACCAAGACCAAt                                                   >  2:2304107/1‑150 (MQ=255)
                                                                                                aCAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATg                                                  <  2:1685471/150‑1 (MQ=255)
                                                                                                 cAACCGTTCTGGTACCAGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGa                                                 <  2:298569/150‑1 (MQ=255)
                                                                                                   aCCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATTGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACg                                               >  1:2600855/1‑150 (MQ=255)
                                                                                                      gTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGAtt                                            >  2:305946/1‑150 (MQ=255)
                                                                                                       ttCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGAttt                                           <  2:949428/150‑1 (MQ=255)
                                                                                                        tCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttc                                          >  2:431644/1‑150 (MQ=255)
                                                                                                         cTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttct                                         <  2:3961073/150‑1 (MQ=255)
                                                                                                          tGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttctt                                        >  2:1338236/1‑150 (MQ=255)
                                                                                                              aCCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATTGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGaa                                    <  2:4023392/150‑1 (MQ=255)
                                                                                                               cccGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGaaa                                   >  1:1899025/1‑150 (MQ=255)
                                                                                                                 cGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatg                                 <  1:3158233/150‑1 (MQ=255)
                                                                                                                 cGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatg                                 <  2:4158361/150‑1 (MQ=255)
                                                                                                                  gTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatga                                >  1:223413/1‑150 (MQ=255)
                                                                                                                  gTAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACACGATGGCAGAAATCGATACAATGGAAGTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatga                                >  2:1792682/1‑150 (MQ=255)
                                                                                                                   tAAAGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatgat                               <  1:883016/150‑1 (MQ=255)
                                                                                                                    aaaGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatgatg                              <  1:148665/150‑1 (MQ=255)
                                                                                                                    aaaGAAGAGCTGCTCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatgatg                              >  1:1635639/1‑150 (MQ=255)
                                                                                                                            gctgctCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTc                      <  2:1291574/150‑1 (MQ=255)
                                                                                                                             ctgctCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCa                     <  1:3419921/150‑1 (MQ=255)
                                                                                                                             ctgctCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCa                     <  1:105277/150‑1 (MQ=255)
                                                                                                                               gctCACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATa                   <  2:1749754/150‑1 (MQ=255)
                                                                                                                                  cACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAt                >  2:2492834/1‑150 (MQ=255)
                                                                                                                                  cACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAt                <  2:627208/150‑1 (MQ=255)
                                                                                                                                  cACGACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAt                <  2:449281/150‑1 (MQ=255)
                                                                                                                                     gACTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTg             <  1:2574140/150‑1 (MQ=255)
                                                                                                                                      aCTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGt            >  1:1001564/1‑150 (MQ=255)
                                                                                                                                      aCTCAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGt            >  1:3284117/1‑150 (MQ=255)
                                                                                                                                         cAGGAAGAACCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCtt         >  2:531988/1‑150 (MQ=255)
                                                                                                                                               gaaCCGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCa   <  2:2230386/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAA  >  AP012306/3396612‑3396905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑