BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,805,954	G→C	intergenic (+153/‑23)	ecnB → / → sugE	entericidin B membrane lipoprotein/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,805,954	0	G	C	100.0%	1425.7 / NA	347	intergenic (+153/‑23)	ecnB/sugE	entericidin B membrane lipoprotein/multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (134/213); total (134/213)

Only 100 of 358 total aligned reads displayed.

AACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCA  >  AP012306/3805807‑3806100

 aCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGcccc                                                                                                                                                 <  1:2934510/150‑1 (MQ=255)

   ggTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCa                                                                                                                                               <  2:1161473/150‑1 (MQ=255)

      aCGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAAtt                                                                                                                                            <  1:3081173/150‑1 (MQ=255)

       cGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATtc                                                                                                                                           >  2:3876461/1‑150 (MQ=255)

        gACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATtct                                                                                                                                          >  1:3416508/1‑150 (MQ=255)

        gACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATtct                                                                                                                                          >  1:3919816/1‑150 (MQ=255)

        gACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATtct                                                                                                                                          >  1:2275292/1‑150 (MQ=255)

         aCAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATtctc                                                                                                                                         >  2:2645805/1‑150 (MQ=255)

         aCAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATtctc                                                                                                                                         <  1:1810433/150‑1 (MQ=255)

          cAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTcc                                                                                                                                        <  1:3335385/150‑1 (MQ=255)

               gtgtCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGc                                                                                                                                   >  1:3529778/1‑150 (MQ=255)

                tgtCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGcc                                                                                                                                  >  1:1000599/1‑150 (MQ=255)

                 gtCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCt                                                                                                                                 <  2:2769933/150‑1 (MQ=255)

                 gtCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCt                                                                                                                                 >  1:3590451/1‑150 (MQ=255)

                  tCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTg                                                                                                                                <  2:3394715/150‑1 (MQ=255)

                  tCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTg                                                                                                                                <  2:1409569/150‑1 (MQ=255)

                  tCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTg                                                                                                                                <  2:2062250/150‑1 (MQ=255)

                   cGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGa                                                                                                                               <  1:4175458/150‑1 (MQ=255)

                   cGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGa                                                                                                                               >  1:1645197/1‑150 (MQ=255)

                   cGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGa                                                                                                                               >  2:2890252/1‑150 (MQ=255)

                    gTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGat                                                                                                                              <  1:678406/150‑1 (MQ=255)

                     tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGata                                                                                                                             >  1:874310/1‑150 (MQ=255)

                      gCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGatat                                                                                                                            <  2:3671727/150‑1 (MQ=255)

                      gCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGatat                                                                                                                            <  2:353212/150‑1 (MQ=255)

                      gCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGatat                                                                                                                            <  1:187857/150‑1 (MQ=255)

                       ccGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATg                                                                                                                           <  2:2223802/150‑1 (MQ=255)

                        cGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGt                                                                                                                          <  1:2080281/150‑1 (MQ=255)

                        cGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGt                                                                                                                          <  2:1320280/150‑1 (MQ=255)

                            tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTg                                                                                                                      <  2:1392239/150‑1 (MQ=255)

                              ttttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGa                                                                                                                    <  1:3719311/150‑1 (MQ=255)

                               tttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGAt                                                                                                                   >  1:1759867/1‑150 (MQ=255)

                                ttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGAtt                                                                                                                  <  2:3964717/150‑1 (MQ=255)

                                ttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGAtt                                                                                                                  <  1:206485/150‑1 (MQ=255)

                                  ttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTAt                                                                                                                <  1:2744605/150‑1 (MQ=255)

                                    cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCt                                                                                                              >  2:3474683/1‑150 (MQ=255)

                                         aTAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGtt                                                                                                         <  2:3152321/150‑1 (MQ=255)

                                         aTAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGtt                                                                                                         <  2:807855/150‑1 (MQ=255)

                                          tAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTa                                                                                                        >  2:3281356/1‑150 (MQ=255)

                                             tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTg                                                                                                     <  2:1059342/150‑1 (MQ=255)

                                             tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTg                                                                                                     <  1:949059/150‑1 (MQ=255)

                                             tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTg                                                                                                     <  2:2711490/150‑1 (MQ=255)

                                             tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTg                                                                                                     >  2:614875/1‑150 (MQ=255)

                                               acaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCt                                                                                                   >  2:3373922/1‑150 (MQ=255)

                                               acaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCt                                                                                                   >  1:2853517/1‑150 (MQ=255)

                                                     gTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTctg                                                                                             >  1:252931/1‑150 (MQ=255)

                                                     gTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTctg                                                                                             <  2:4076881/150‑1 (MQ=255)

                                                        aTAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTctgctg                                                                                          >  2:2845889/1‑150 (MQ=255)

                                                         tAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTgg                                                                                         <  1:569236/150‑1 (MQ=255)

                                                         tAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTCGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTgg                                                                                         <  1:155272/150‑1 (MQ=255)

                                                             tGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGt                                                                                     <  1:1524990/150‑1 (MQ=255)

                                                              gAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTg                                                                                    <  1:82400/150‑1 (MQ=255)

                                                               aaaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGACTGCTGGAAGTgg                                                                                   >  2:2990983/1‑150 (MQ=255)

                                                                aaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGt                                                                                  >  2:3498772/1‑150 (MQ=255)

                                                                 aTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTa                                                                                 <  1:1367524/150‑1 (MQ=255)

                                                                  ttCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTAt                                                                                <  2:1266423/150‑1 (MQ=255)

                                                                    ccccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                                                              >  1:504726/1‑150 (MQ=255)

                                                                    ccccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                                                              >  1:2154509/1‑150 (MQ=255)

                                                                    ccccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                                                              >  1:2371020/1‑150 (MQ=255)

                                                                       cTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGcc                                                                           >  1:404279/1‑150 (MQ=255)

                                                                          ccACCTGGCAAAATATCGGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGtt                                                                        <  2:955327/150‑1 (MQ=255)

                                                                            aCCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTgg                                                                      >  1:2621330/1‑150 (MQ=255)

                                                                            aCCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTgg                                                                      >  1:558612/1‑150 (MQ=255)

                                                                                ggCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGTCCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTg                                                                  <  2:894209/150‑1 (MQ=255)

                                                                                ggCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTg                                                                  >  1:1410633/1‑150 (MQ=255)

                                                                                  cAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaa                                                                >  1:1028990/1‑150 (MQ=255)

                                                                                    aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAat                                                              >  2:1805553/1‑150 (MQ=255)

                                                                                    aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAat                                                              <  1:1096528/150‑1 (MQ=255)

                                                                                    aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAat                                                              >  2:3025474/1‑150 (MQ=255)

                                                                                     aaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAata                                                             >  1:3895924/1‑150 (MQ=255)

                                                                                      atatCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAatat                                                            <  1:3126702/150‑1 (MQ=255)

                                                                                         tCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAcc                                                         <  1:1606585/150‑1 (MQ=255)

                                                                                         tCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAcc                                                         <  1:3241551/150‑1 (MQ=255)

                                                                                          ccGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAccc                                                        >  2:3620300/1‑150 (MQ=255)

                                                                                            gTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCAc                                                      <  2:2508082/150‑1 (MQ=255)

                                                                                            gTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCAc                                                      >  2:531580/1‑150 (MQ=255)

                                                                                              tCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACgg                                                    <  1:728550/150‑1 (MQ=255)

                                                                                               cAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGc                                                   >  1:4217731/1‑150 (MQ=255)

                                                                                               cAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGc                                                   >  2:494831/1‑150 (MQ=255)

                                                                                                   cATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTa                                               >  1:927652/1‑150 (MQ=255)

                                                                                                     tCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGt                                             <  1:1698340/150‑1 (MQ=255)

                                                                                                         cTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt                                         >  2:450055/1‑150 (MQ=255)

                                                                                                          tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGttt                                        <  1:2785832/150‑1 (MQ=255)

                                                                                                            tGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGa                                      <  1:2007005/150‑1 (MQ=255)

                                                                                                              cAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACg                                    <  1:3191281/150‑1 (MQ=255)

                                                                                                              cAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACg                                    >  1:1457631/1‑150 (MQ=255)

                                                                                                                       cTGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGtt                           <  2:3938674/150‑1 (MQ=255)

                                                                                                                        tGCAAACGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGtta                          <  1:343208/150‑1 (MQ=255)

                                                                                                                           aaaCGCCTCTTTTCACCGGGAACGCCCCCAATTCTCCGGAGCCTTATATTTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGttatta                       <  2:1028788/150‑1 (MQ=255)

                                                                                                                            aaCGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTAc                      <  1:2987207/150‑1 (MQ=255)

                                                                                                                            aaCGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTAc                      <  2:4023840/150‑1 (MQ=255)

                                                                                                                             aCGCCTCTTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACt                     <  2:2413342/150‑1 (MQ=255)

                                                                                                                                  tctTTTCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGAc                <  2:2583378/150‑1 (MQ=255)

                                                                                                                                     tttCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGTCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGc             <  2:1711183/150‑1 (MQ=255)

                                                                                                                                     tttCACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGc             >  2:2934741/1‑150 (MQ=255)

                                                                                                                                     ttgcACCGGGGACGCCACCAATTCCCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTCTGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGc             <  1:1213206/147‑1 (MQ=255)

                                                                                                                                        cACCGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCgat          >  2:596959/1‑150 (MQ=255)

                                                                                                                                           cGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCgatgat       <  1:893641/150‑1 (MQ=255)

                                                                                                                                           cGGGGACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCgatgat       <  2:1574804/150‑1 (MQ=255)

                                                                                                                                               gACGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTc   <  2:2111426/150‑1 (MQ=255)

                                                                                                                                                aCGCCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCa  <  1:364273/150‑1 (MQ=255)

AACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCA  >  AP012306/3805807‑3806100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`