Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG32.6% 173.6 / 23.4 99G60G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (13/53);  new base G (32/0);  total (45/54)
Fisher's exact test for biased strand distribution p-value = 1.11e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGG  >  AP012306/341228‑341523
                                                                                                                                                        |                                                                                                                                                   
 tAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTg                                                                                                                                                    <  1:3394259/150‑1 (MQ=255)
  aaaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGa                                                                                                                                                   <  1:3093152/150‑1 (MQ=255)
   aaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTgga                                                                                                                                                  >  1:3921271/1‑148 (MQ=255)
    aGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTggga                                                                                                                                                 >  2:1042362/1‑147 (MQ=255)
     gCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGgaaa                                                                                                                                                >  1:2100146/1‑150 (MQ=255)
     gCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGaaaa                                                                                                                                                <  2:3608205/150‑1 (MQ=255)
       tGGCGCAACGAGGACGTTTTACCACGCATCCCACCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGT‑C‑GCGTGAAAAAg                                                                                                                                              <  2:4031704/150‑1 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGcc                                                                                                                                            <  2:1946516/150‑1 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGcc                                                                                                                                            <  1:2019334/150‑1 (MQ=255)
          cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCC‑GGGGGAAAAAGcc                                                                                                                                            >  1:4037017/1‑150 (MQ=255)
          cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCa                                                                                                                                           <  1:3731922/150‑1 (MQ=255)
          cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCa                                                                                                                                           <  1:1555957/150‑1 (MQ=255)
          cccagcGAGGACGTATTACCACGCATCCCAGCCCGAATGTCGGGTGCGTCAATGTCAAAGATGGCGAAATTGTCGGTGAGGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTCCACGCGTTGCGTATGG‑A‑GGGTGAAAAAGcta                                                                                                                                           <  2:1673990/145‑3 (MQ=255)
            cAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCaaa                                                                                                                                         <  2:3338040/150‑1 (MQ=255)
            cAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAAACaaa                                                                                                                                         >  2:2683983/1‑150 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAg                                                                                                                                        <  2:2458533/150‑1 (MQ=255)
              aCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCaaaag                                                                                                                                       >  1:3674344/1‑148 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAGGCaaaaggg                                                                                                                                     >  1:2248473/1‑146 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCaaaaggg                                                                                                                                     >  2:2671265/1‑146 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGACaaaaagg                                                                                                                                     >  2:2052311/1‑146 (MQ=255)
                 aGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGc                                                                                                                                    <  1:1927056/150‑1 (MQ=255)
                  ggACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑CGGGGGGAAAAAGCCaaaaaggg                                                                                                                                    >  2:2886486/1‑145 (MQ=255)
                  ggACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCg                                                                                                                                   <  1:2330309/150‑1 (MQ=255)
                  ggACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCg                                                                                                                                   <  2:681062/150‑1 (MQ=255)
                  ggACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCg                                                                                                                                   <  2:454265/150‑1 (MQ=255)
                   gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGa                                                                                                                                  <  2:379366/150‑1 (MQ=255)
                   gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGCgg                                                                                                                                  >  2:560011/1‑149 (MQ=255)
                   gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCCAAAGTGCGa                                                                                                                                  >  1:1021216/1‑150 (MQ=255)
                     cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGAcc                                                                                                                                <  1:182133/150‑1 (MQ=255)
                     cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGACAAAGGAGCGaac                                                                                                                                >  2:207487/1‑148 (MQ=255)
                     cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAAACAAAAGAGCGGcc                                                                                                                                >  1:2704993/1‑150 (MQ=255)
                          tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTa                                                                                                                           <  1:1555381/150‑1 (MQ=255)
                           aCCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAGG‑GCGACCGCCCATg                                                                                                                         >  1:1780279/1‑150 (MQ=255)
                                  aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGCGGCCGCCTCTGTCacac                                                                                                                   >  1:3511745/1‑150 (MQ=255)
                                     ccAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGGAAAAACCAAAAGGGCGAACCCCTATGTcacaaccc                                                                                                                >  1:803645/1‑146 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGGAAAAGCCAAAGGGGCGACCGCCTATGTCCCACTCG‑aa                                                                                                             >  1:1690619/1‑150 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCCAAAGCGCGGCCGCCTATGTCACACTCC‑aa                                                                                                             >  1:466709/1‑150 (MQ=255)
                                        cccagattgtgGGGTGTGTATTTGAAAAGGAGGGCGAAATTGTGGGTGATGGTTTCCACGAGC‑GGGCGGGGGAACCACATGCGGAAGTCCACGCGTTGGTTGGGG‑G‑GGGTGAAAAACCAAAGGGTGCGACCGCCTATGTCACACTCG‑AAc                                                                                                            <  2:3476492/143‑1 (MQ=255)
                                        aCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCCAAAGTGCGACCGCCTATTTCACACCCG‑TAc                                                                                                            >  1:672327/1‑150 (MQ=255)
                                          ccGAATGTCGGGTGCGTCATTGTAAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AAccc                                                                                                          <  1:2518440/150‑1 (MQ=255)
                                           cGAATGTCGGGTGCGTGATTGTCAAAGATGGCAAATTTGTCGGTGAAGGTTCCCCCCACC‑GTGGGGGAGAACCATAGGCCGAAGTCCCCGCGTGGCGTGGGG‑C‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCt                                                                                                         <  2:2134258/150‑1 (MQ=255)
                                           cGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAGACAAAAGGGCGGCCGCCCATGTCACACTCGCAAccc                                                                                                          >  2:368133/1‑150 (MQ=255)
                                                  cGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCca                                                                                                  <  1:479615/150‑1 (MQ=255)
                                                   gggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAAGGGCGACCGCCCATGTCACACTCG‑AAACCCGTAGCcat                                                                                                 >  1:1257815/1‑150 (MQ=255)
                                                     gTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑CGGGGGGAAAAACCCAAAGG‑GGGACCCCCCATGTCACCCCCC‑AAACCTGTTGcccgca                                                                                               >  2:1233524/1‑146 (MQ=255)
                                                      tGCGTCATGGTAAAAGAGGGCGAAATTGTCGGGGAA‑GTTTCCCCCAACGGGGCGGGTGAACCACGTCCCGAAGTCCGCGCGTTGCGTAGGG‑C‑GGGTGAAAAGGCAAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCcatcat                                                                                              <  2:841866/150‑1 (MQ=255)
                                                       gCGTCATTGTAAAAGATGGCAAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTCCACGCGTTGCGTATGG‑C‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATg                                                                                             <  2:3280473/150‑1 (MQ=255)
                                                         gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGt                                                                                           <  1:2271549/150‑1 (MQ=255)
                                                         gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGGAAAAACCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGt                                                                                           >  1:3566944/1‑150 (MQ=255)
                                                          tCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTc                                                                                          <  1:1477533/150‑1 (MQ=255)
                                                              tGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTAc                                                                                      <  1:3344604/150‑1 (MQ=255)
                                                               gaaaaaGATGGAGAAGTTGTCGGTGAAGGTCACCACAACG‑GGGGGGGTAAACCACATGCCGAACTACCCCGGTTGCGTAGGG‑C‑GGGTAAAAAGGCCAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACg                                                                                     <  2:1099430/147‑1 (MQ=255)
                                                               gTCAAAGAGGGGGAATTTGTCGGTGAAGGTTCCCACCAAC‑GTGGGGGTGACCCACATGCCGAAGTACCCGCGTTGCGTAGGG‑C‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACg                                                                                     <  2:226399/150‑1 (MQ=255)
                                                                 cAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAAGCAAAGGGGCGGCCCCCCATGTTACACTCG‑CAACCTTTAGGCATCATGGTTGTACGcc                                                                                   >  2:3292279/1‑150 (MQ=255)
                                                                    aGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGTGCGACCGCCCATGTCACACTCG‑AACCCCGTAGCCATCATGGTCGGACGCCAcc                                                                                >  2:906444/1‑150 (MQ=255)
                                                                      aTGGCAAAATTGCCGGTGAAGGTTCCCACCACC‑GTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTAGGG‑G‑GGGTGAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGt                                                                              <  2:20637/150‑1 (MQ=255)
                                                                       tGGCGAAATTGTCGGTGAAGGTTACCACCACC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                             <  1:20994/150‑1 (MQ=255)
                                                                        ggCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAAACAAAAGTGGGACCGCCCATGTCACACTCC‑AACCCCTTTGCCCTCAATGTCGTACGCCCCCCTGc                                                                            >  2:483020/1‑150 (MQ=255)
                                                                         gaaaatttGTGGGTGAAGTTCCCCCCCAAG‑GGGGGGGAGAACCACAGGCGGAAGTACACGCGTGGCGTAGGG‑G‑GGGTAAAAAAGCAAAGGGTGCGCCCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCt                                                                           <  2:4049901/144‑1 (MQ=255)
                                                                          caaaaTGGTCGGGGAAGGTTCCCACCAAC‑GGGCGGGTAACCAACATGCGGAAGTACACGCGTGGCGTAGGG‑G‑GGGGAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtg                                                                          <  2:3381247/148‑1 (MQ=255)
                                                                           gAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCG‑AACCCCGTAGCCATCATGGTCGTACGCCCCCGTGCcgt                                                                         >  2:2650033/1‑150 (MQ=255)
                                                                            aaaTTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGAGCGAACCCCCATGTTACAACCC‑AACCCCCTAGCCCTCCTGGGCGTACGCCCCCCCGCtgtt                                                                        >  2:147456/1‑149 (MQ=255)
                                                                            aaaTGGTGGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTAGGG‑C‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgtg                                                                        <  2:2317620/150‑1 (MQ=255)
                                                                               ttGTGGGTGAAGGTTACCACCAAC‑GGGCGGGTGAACCAAATGCCGAAGTACACGCGTGGCGTATGG‑G‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg                                                                     <  2:1491423/150‑1 (MQ=255)
                                                                               ttGTCGGTGAGGGTTACCACAAAG‑GGGGGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg                                                                     <  2:422934/150‑1 (MQ=255)
                                                                                   cGGTGAAGGTTACCACCAAC‑GAGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGGAAAAACCAAAAAGGGGACCGCCCATGTCACACTCG‑AAACCTGTAGCCATAATAGGCGTACGCCACCCGGCtgtggccgcac                                                                 >  2:695271/1‑143 (MQ=255)
                                                                                   cGGTGAAGGCTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAAACAAAAGGGCGACCGCCCATGTTCCACTCG‑CACCCCGTAGACAACCAGGTCGTACGCCCCCCTGCTGTGAcccccc                                                                 >  2:1220883/1‑145 (MQ=255)
                                                                                     gTGAAGGTTACCCCCAAC‑GTGGGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGG‑G‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCa                                                               <  2:3318985/150‑1 (MQ=255)
                                                                                       gaggtttACCACCAAC‑GTGGGGGGGAACCACATCGCGAAGTACACGCGTTGCGGATGG‑G‑GGGTGAAAACCCCAAAGGTGCGCCCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                             <  2:1797188/145‑1 (MQ=255)
                                                                                       gAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑G‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                             <  1:105096/150‑1 (MQ=255)
                                                                                        aaGGTTCCCACCACC‑GTGCGGGTCAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTAAAAAACCCAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcg                                                            <  2:3809157/150‑1 (MQ=255)
                                                                                          ggTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgcc                                                          <  1:1260446/150‑1 (MQ=255)
                                                                                               ccaccaAC‑GTGGGGGTGAACCACATGCGGAAGTACACGGGTTGCGTAGGG‑G‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTgg                                                     <  1:2843575/150‑1 (MQ=255)
                                                                                                caccacC‑GGGGGGGTGAACCAAATGCCGAAGTACATGCGTTGCGTAGGG‑G‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGc                                                    <  2:3853961/150‑1 (MQ=255)
                                                                                                 cccaAC‑GTGCGGGAGAACCACTTGCCAAAGTCCCCGCGTCGCGTATGG‑G‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCg                                                   <  2:1359109/149‑1 (MQ=255)
                                                                                                   caAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGCGACCCCCCATGTCCCACCCC‑AACCCCGTAGCCCTCAAGGGCGGACGCCCCCCGGCTGTGACCCACCCATCCCCCCTGGCGTa                                                 >  2:639502/1‑150 (MQ=255)
                                                                                                      c‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGCGGCCCCCCATGTCACACTCC‑AACCCCGTCACCAACATGGTCCGACGCCCCCGTGCTGTGACGCACTCATCGCCCCTcgcggggcg                                              >  2:3024606/1‑145 (MQ=255)
                                                                                                           cGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGCGACCGCCCATGTCCCACCCG‑AACCCCGTAGCCATCATTGTCGTACGCCACCCGGCTGTGACGCACTCCTCCCCGCCCGCGTAGcgcgcg                                          >  2:706475/1‑150 (MQ=255)
                                                                                                            gggTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGGAAAAACCAAAAGGGCGGCCCCCCCTGTCACACCCG‑CACCCCGTCGCCATCATTGGCGTACTCCACCCGGCTGTGACGCACTCCCCGCCGCTGGCGTAGCGCGCGt                                         >  2:2811663/1‑150 (MQ=255)
                                                                                                            gggTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAAGGGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGt                                         >  1:1010686/1‑150 (MQ=255)
                                                                                                               tGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCCAAAAAGCGACCGCCCTTCTCACACTCG‑AACCCCCGAGCCATCATGGTGCTACGCCACCCTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCggggc                                      >  2:1026634/1‑149 (MQ=255)
                                                                                                                 aaCCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAAGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTg                                    >  1:1359521/1‑150 (MQ=255)
                                                                                                                     acaTGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                <  1:3466968/150‑1 (MQ=255)
                                                                                                                      caTGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAAGGGCCACCCCCCCTGTCACACTCC‑AACCCCCTAGCCCTCATGGGCGTACGCCACCCGGCTGTGGCCCACTCCTCCCCCCTTGCGGAGCGCGCGGGGGTGccccc                               >  2:1189771/1‑147 (MQ=255)
                                                                                                                       aTGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAAGGGCGACCGCCCATGTCACACCCG‑AACCCTGTAGGCCTCAAGGTCGTACGCCACCCGGCTGTGACGCACCCATCGCCCCCGGCGTAGCGCGCGTGGTTGCCTcca                              >  2:3249760/1‑148 (MQ=255)
                                                                                                                           cGAAGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAAGGGGGACCGCCCATGTCACACTCG‑AACCCTGTAGCCCTCATGGTCGTACGCCACCCCGCTGTGGCGCACTCATCGCCGCCGGGGTAGCGCGCGTGGTTGCCTCGCTGCa                          >  2:1504190/1‑150 (MQ=255)
                                                                                                                             aaGTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCCAAAGGGGGACCCCCCCTTTCACACTCC‑AACCCCGTAGCCACCATGGGCGGACGCCCCCCCGCTGTGACGCACCCATCGCCGCCGGCGTAGCGCGCGTGGTTGCCTCGATGCaaa                        >  2:2609080/1‑149 (MQ=255)
                                                                                                                               gTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAGCCAAAGGAGGGACCGCCCATGTCCCACTCC‑AACCCTGTAGCCATCATGGTCGTACGCCACCGGGCTGTGACGCACTCATCGCCGCTGGCGGAGCGCGCGTGGGTGCCTCGATGCAAAAt                      >  2:1985385/1‑150 (MQ=255)
                                                                                                                               gTACACGCGTTGCGTATGG‑C‑GGGGGAAAAAACCAAAAGGGCGGCCCCCCATGTCCCACTCG‑CACCCTGTAGCCATCATGGTCGTACGCCACCCTGCTGTTACGCCCTCATCCCCGCCGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGaa                      >  2:1814289/1‑149 (MQ=255)
                                                                                                                                  cacGCTTTGCGTATGG‑G‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                   <  1:2202804/150‑1 (MQ=255)
                                                                                                                                  cacGCGTGGCGAAGGG‑G‑GGGTAAAAAACCAAAAGGTCCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                   <  2:2157809/150‑1 (MQ=255)
                                                                                                                                     gcgTTGCGTATGG‑C‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAc                <  1:2602664/150‑1 (MQ=255)
                                                                                                                                     gcgTTGCGTAGGG‑C‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAc                <  2:3797746/150‑1 (MQ=255)
                                                                                                                                      cgTTGCGTGGGG‑G‑GGGTGAAAAGGCAAAGGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAcc               <  1:724967/150‑1 (MQ=255)
                                                                                                                                         tgggaaggg‑C‑GGGTAAAAAGGCGAAGGGTGGGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGc            <  2:2380965/143‑1 (MQ=255)
                                                                                                                                         tGCGTATGG‑C‑GGGTGGAAAAACCAAAGGTGCGACCGCCCCTCTCACACCCC‑AACCCTCTAGCCATCATGGTCGTACGCCACCCTGCTGTGAAGCACTCACCGCCCCCGGCGGAGCGCGCGTGGGTGCCCCGATGCAAGATCCCAAccccc            >  2:2924583/1‑148 (MQ=255)
                                                                                                                                          gCGTATGG‑C‑GGGTGAAAAAAACAAACGGGAGCCCCCCCACTGCACACTCC‑GACCCTTGAGCCAACATGGGCGTTCGCCACCGTGCTCGGACGCACTCATCGCCGCCGGCCGAGCGCGCGGGGTTGCCTCCAAGCAAGATCCTAAaccccc           >  2:3959381/1‑147 (MQ=255)
                                                                                                                                            gTATGG‑C‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAgg         <  1:799152/150‑1 (MQ=255)
                                                                                                                                               tGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCg      >  1:95/1‑150 (MQ=255)
                                                                                                                                                     gggTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTgg  >  1:263297/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                   
CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAC‑GTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGG‑C‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCG‑AACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGG  >  AP012306/341228‑341523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑