Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306800,6750AC29.3% 533.9 / 40.3 240T192P (ACC→CCC) lolAchaperone for lipoproteins
Reads supporting (aligned to +/- strand):  ref base A (137/32);  new base C (0/70);  total (137/103)
Fisher's exact test for biased strand distribution p-value = 9.20e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 292 total aligned reads displayed.

GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824
                                                                                                                                                      |                                                                                                                                                     
gCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑Ca                                                                                                                                                       <  1:704470/150‑1 (MQ=255)
    tGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CAcccc                                                                                                                                                   <  1:2012832/150‑1 (MQ=255)
       cAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCccgcc                                                                                                                                                <  2:163438/150‑1 (MQ=255)
          tCTGAAGCAGTTCACAATTAACGGGGGACGGGATGGCACAATCCATCAGTTTAGCGCGGTGGAGGAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAATGGGGCTGGGGATGCAGAGAATTTTCCCTC‑CCCCCCGCCGCa                                                                                                                                             <  2:3584633/150‑1 (MQ=255)
           cTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCaa                                                                                                                                            >  2:2337671/1‑150 (MQ=255)
              aaGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGc                                                                                                                                         >  1:2865338/1‑150 (MQ=255)
              aaGCAGTTCACCATTAACGTGGGACGTGATGGCACAACCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAACCCCAGAAAAAGGGGGCTGTGGATGCAGCAAAATTTACCTT‑CCCCCCGCCGCAAGGc                                                                                                                                         <  2:599430/150‑1 (MQ=255)
               aGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCg                                                                                                                                        <  2:3797729/150‑1 (MQ=255)
                gCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGt                                                                                                                                       >  1:518700/1‑150 (MQ=255)
                ccAGTCCACCATTAACGTGGGACGTGATGGCAAAATCCATCAGTTTAGCGCGGGGGAGAAGGACGATCAGCGCAGCAGTTATCACCTGAAACCCCAGAAAAAGGGGGCTGGGGATGCAGCGAAATTTCCCTC‑CCCCCCGCCGCAAGGCGt                                                                                                                                       <  2:365219/149‑1 (MQ=255)
                 cAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTc                                                                                                                                      >  1:787510/1‑150 (MQ=255)
                 cAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTc                                                                                                                                      >  1:3393146/1‑150 (MQ=255)
                   gTTCACCATTACCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCAc                                                                                                                                    <  2:3531122/150‑1 (MQ=255)
                     tCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACgg                                                                                                                                  <  1:3881586/150‑1 (MQ=255)
                           ttAGCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCGGGACGATCAGCGCAGCAGTTATCAACTAAAATCCAAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAgatg                                                                                                                            <  1:561736/150‑1 (MQ=255)
                           ttAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAgatg                                                                                                                            <  1:1531967/150‑1 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                          >  2:3117998/1‑150 (MQ=255)
                               cGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCa                                                                                                                        >  2:2527829/1‑150 (MQ=255)
                                gTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCaa                                                                                                                       >  1:3375606/1‑150 (MQ=255)
                                     aCGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCAAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTaa                                                                                                                  <  2:1368704/150‑1 (MQ=255)
                                      cGTGATGGCAAAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGAGGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAg                                                                                                                 <  1:1706994/150‑1 (MQ=255)
                                       gTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGt                                                                                                                >  2:2757900/1‑150 (MQ=255)
                                        tGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTa                                                                                                               >  2:289559/1‑150 (MQ=255)
                                          aTGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCAAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTaga                                                                                                             <  1:992435/150‑1 (MQ=255)
                                           tGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGAGGCAGCAAATTTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTagag                                                                                                            <  2:3860714/150‑1 (MQ=255)
                                              cacaATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCa                                                                                                         >  1:1410219/1‑150 (MQ=255)
                                              cacaATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCa                                                                                                         >  1:1159898/1‑150 (MQ=255)
                                                caATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAcc                                                                                                       >  1:3106985/1‑150 (MQ=255)
                                                caACCCATCAGTTTAGCGCGGTGGAGAAGGACGATCAGCGCAGCAGTTATCAACTAAACCCCCAGCAAAATGGGGCTGTGGATGAAGCGAAATTTACCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAcc                                                                                                       <  2:1220506/150‑1 (MQ=255)
                                                  aTCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg                                                                                                     <  1:161470/150‑1 (MQ=255)
                                                    ccATCATTTAAGCGCGGTGGGGCAGGCCAATCGGCCCAGAATTTATCAACTGAAATCCCAGAAAATGGGGGTTGTGGACGCAGCGAATTTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag                                                                                                   <  2:657991/150‑1 (MQ=255)
                                                    ccATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag                                                                                                   >  2:933068/1‑150 (MQ=255)
                                                     cATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagt                                                                                                  >  1:1366600/1‑150 (MQ=255)
                                                       tCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAATGGGGTTGGGGATGCAGAGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtga                                                                                                <  2:829000/150‑1 (MQ=255)
                                                        ccgTTTACCGCGGTGGAGCGGGCCGATCCCCCCCGCATTTACCAACTCAAACCCCAGCAAAATGGGGCTGTGTCTCCCCCCAAATTTCCCTC‑CCCCCCGCCCCAAGGCGTCCCGGTAGATGATCAACGTAAGTAGAGGCACCtgagtgag                                                                                               <  2:3173035/148‑1 (MQ=255)
                                                        cAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtgag                                                                                               <  1:858173/150‑1 (MQ=255)
                                                         aGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGc                                                                                              >  2:2039958/1‑150 (MQ=255)
                                                               gcgcGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTg                                                                                        >  1:3632807/1‑150 (MQ=255)
                                                               gcgcGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTg                                                                                        >  2:1695834/1‑150 (MQ=255)
                                                               gcgcGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTg                                                                                        <  2:1106516/150‑1 (MQ=255)
                                                                    gTGGAGCAGGACGACCAGCGCAGCAGTTATCAACTAAAACCCCAGAAAAATGGGGCTGGGGATGCAGCAAAATTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt                                                                                   <  2:3560079/150‑1 (MQ=255)
                                                                     tGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTc                                                                                  >  2:282612/1‑150 (MQ=255)
                                                                      ggAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCg                                                                                 <  2:1025221/150‑1 (MQ=255)
                                                                        aGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAt                                                                               <  1:2319199/150‑1 (MQ=255)
                                                                        aGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAt                                                                               >  1:3791133/1‑150 (MQ=255)
                                                                         gCAGGCCGATCAGCGCAGCAGTTATCACGTCAAACCCCAGAAAAATGGGGCTGTGGAGGCGGCGAAATTTCCTTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAtt                                                                              <  2:1729837/150‑1 (MQ=255)
                                                                          cAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                             >  1:1033325/1‑150 (MQ=255)
                                                                          cAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                             <  1:3935696/150‑1 (MQ=255)
                                                                            ggACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttttt                                                                           <  2:3486364/150‑1 (MQ=255)
                                                                               cGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCgg                                                                        >  2:3390409/1‑150 (MQ=255)
                                                                                gATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGa                                                                       >  2:4049432/1‑150 (MQ=255)
                                                                                gATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGa                                                                       >  1:2603525/1‑150 (MQ=255)
                                                                                   cAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataa                                                                    <  1:2293533/150‑1 (MQ=255)
                                                                                    aGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                   <  2:1285933/150‑1 (MQ=255)
                                                                                      cgcAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATAc                                                                 >  2:2061192/1‑150 (MQ=255)
                                                                                        cagcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtt                                                               <  2:3904754/150‑1 (MQ=255)
                                                                                        cagcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGTTAATACtt                                                               >  2:3744733/1‑150 (MQ=255)
                                                                                        cagcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtt                                                               <  1:2895479/150‑1 (MQ=255)
                                                                                        cagcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtt                                                               >  1:2799805/1‑150 (MQ=255)
                                                                                          gcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtttt                                                             <  1:1567901/150‑1 (MQ=255)
                                                                                          gcagTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCATCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtttt                                                             <  1:2254493/150‑1 (MQ=255)
                                                                                           cagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTc                                                            >  1:1689675/1‑150 (MQ=255)
                                                                                               tATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAcc                                                        >  2:191609/1‑150 (MQ=255)
                                                                                               tATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAcc                                                        >  2:1142359/1‑150 (MQ=255)
                                                                                                aTCAACTGAAATCCCAGCAAAATGGGGCTGTGGAAGAAGAGAAAGTAACCTT‑CACCCCGCCGAAAGGCGGCACGGGAGATGATCAAGGTAAGGAGAGGAACCTGAGCGAGCAATATGTCGGTCGATTTTTTGGATAATACGTTTCACCct                                                       >  2:3081464/1‑150 (MQ=255)
                                                                                                aTCAACTAAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAATTTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACct                                                       <  2:1988357/150‑1 (MQ=255)
                                                                                                  cAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctct                                                     >  2:1803620/1‑150 (MQ=255)
                                                                                                  cAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctct                                                     <  2:2956629/150‑1 (MQ=255)
                                                                                                     cTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGc                                                  <  1:1804505/150‑1 (MQ=255)
                                                                                                     cTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGc                                                  >  1:352441/1‑150 (MQ=255)
                                                                                                         aaccccAGCAAAATGGGGCTGTGGATGCAGCAAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcgcg                                              <  2:901449/147‑1 (MQ=255)
                                                                                                             ccAGCAAAAGGGGGTTGTGGATGCAGCGAAATTTCCCTT‑CGCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGTGCGTa                                          <  1:3246124/150‑1 (MQ=255)
                                                                                                              cAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTAGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTAt                                         >  2:451010/1‑150 (MQ=255)
                                                                                                              cAGCAAAATGGGGCTGTGGATGCAGCAAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTAt                                         <  2:2317085/150‑1 (MQ=255)
                                                                                                               aGCAAATTGGGCGTGGGGATGCGGCAAAATTTGCCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATg                                        <  1:2040914/150‑1 (MQ=255)
                                                                                                               aGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATg                                        <  1:763382/150‑1 (MQ=255)
                                                                                                                gaaaaaGGGGGCTGTGGATGCAGCAAAATTTCCCTC‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGc                                       <  2:711026/148‑1 (MQ=255)
                                                                                                                gCAAAATGGGGCTGGGGATGCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGc                                       <  2:3258530/150‑1 (MQ=255)
                                                                                                                   aaaTGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGc                                    >  2:2574161/1‑150 (MQ=255)
                                                                                                                      tGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAg                                 <  1:3342855/150‑1 (MQ=255)
                                                                                                                       ggggCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGa                                <  1:125599/150‑1 (MQ=255)
                                                                                                                         ggCTGGGGATGCAGC‑AAATTTTCCTTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaaa                              <  2:2987082/150‑1 (MQ=255)
                                                                                                                          gCTGTGGATGCAGCGAAATTTACCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaaaa                             <  1:3705355/150‑1 (MQ=255)
                                                                                                                          gCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaaaa                             >  2:1264437/1‑150 (MQ=255)
                                                                                                                            tgtgGATGCAGCAAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAtt                           <  2:1434210/150‑1 (MQ=255)
                                                                                                                             gtgGATGCAGCAAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAttt                          <  2:1579052/150‑1 (MQ=255)
                                                                                                                              tgGATGCAGCGAAATTTCCCTA‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTa                         <  2:1253045/150‑1 (MQ=255)
                                                                                                                                gATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGc                       >  1:3330350/1‑150 (MQ=255)
                                                                                                                                   gCAGCGAAATTTCCCTT‑CCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGcaca                    <  2:767139/150‑1 (MQ=255)
                                                                                                                                        gAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtata               >  1:2980709/1‑150 (MQ=255)
                                                                                                                                         aaaTTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTatat              >  2:2136186/1‑150 (MQ=255)
                                                                                                                                          aaTTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATc             <  1:2143803/150‑1 (MQ=255)
                                                                                                                                           aTTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCg            >  1:1930839/1‑150 (MQ=255)
                                                                                                                                            tttACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCgg           <  1:3472814/150‑1 (MQ=255)
                                                                                                                                            tttACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGAAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCgg           >  2:2282913/1‑150 (MQ=255)
                                                                                                                                              tACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGcc         >  1:2395396/1‑150 (MQ=255)
                                                                                                                                              tACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGcc         >  2:2712006/1‑150 (MQ=255)
                                                                                                                                              tACCTC‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGcc         <  1:1461936/150‑1 (MQ=255)
                                                                                                                                                ccTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAg       >  2:2164862/1‑150 (MQ=255)
                                                                                                                                                 cTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc      <  1:3428623/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTT‑CACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACA  >  AP012306/800526‑800824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑