Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT32.1% 165.9 / 45.8 84intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (19/38);  new base T (27/0);  total (46/38)
Fisher's exact test for biased strand distribution p-value = 5.70e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 155 total aligned reads displayed.

GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203
                                                                                                                                                       |                                                                                                                                                             
ggCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttg                                                                                                                                                               >  1:1698274/1‑150 (MQ=255)
  cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttttt                                                                                                                                                             >  1:3694360/1‑150 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gttttttttg                                                                                                                                                          >  2:3345254/1‑148 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gttttttttg                                                                                                                                                          >  2:2589073/1‑148 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gttttttttg                                                                                                                                                          >  1:925564/1‑148 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Attttttttg                                                                                                                                                          >  2:3126670/1‑148 (MQ=255)
        tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTggtt                                                                                                                                                        >  2:1820642/1‑148 (MQ=255)
        tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCtt                                                                                                                                                       <  1:2399766/150‑1 (MQ=255)
        tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCtt                                                                                                                                                       <  2:2553957/150‑1 (MQ=255)
        tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCtt                                                                                                                                                       >  2:2990629/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTc                                                                                                                                                      >  1:3324789/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTc                                                                                                                                                      >  1:1642526/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGCTTc                                                                                                                                                      >  1:1245849/1‑150 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTct                                                                                                                                                     >  1:682423/1‑149 (MQ=255)
           gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGGTTtag                                                                                                                                                    >  2:2537019/1‑150 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTcagc                                                                                                                                                   >  1:2748158/1‑150 (MQ=255)
             ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TCTTTTTTGTCTTctgca                                                                                                                                                  >  2:2965569/1‑150 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGATTTTGTTTGGTTTcgccc                                                                                                                                                  >  2:508466/1‑146 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTctgcag                                                                                                                                                 >  2:887517/1‑150 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTcagcag                                                                                                                                                 <  1:4034421/150‑1 (MQ=255)
               gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTcacccgc                                                                                                                                                >  1:357382/1‑145 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGTTTcgccgcgg                                                                                                                                               >  2:3568767/1‑143 (MQ=255)
                  gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCTGCAGC‑ggg                                                                                                                                            >  1:2514570/1‑150 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCGTCGGCAGC‑GGGt                                                                                                                                           >  2:2485913/1‑150 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAGC‑gggg                                                                                                                                           >  1:3528236/1‑149 (MQ=255)
                     tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTg                                                                                                                                         <  1:2270293/150‑1 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTgc                                                                                                                                        <  1:3322985/150‑1 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCAGCAGC‑GGGGTgc                                                                                                                                        >  1:2928038/1‑150 (MQ=255)
                       tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGCTTCCACAAC‑GGGGTgtg                                                                                                                                       >  2:1463676/1‑148 (MQ=255)
                         gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCCGC‑GGGGTTCGCt                                                                                                                                     >  1:2507345/1‑150 (MQ=255)
                           ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑cc                                                                                                                                  <  1:3825525/150‑1 (MQ=255)
                            tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑ccc                                                                                                                                 <  2:1373749/150‑1 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCt                                                                                                                                <  1:1622964/150‑1 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCCGCAGC‑GGGGTGCGCT‑cccct                                                                                                                               >  2:3219653/1‑148 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGGTTCCCCGGC‑GGGCTTCGCT‑cccct                                                                                                                               >  1:3552799/1‑148 (MQ=255)
                               tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTCAGCAGC‑GGGTTGCGCG‑cccctt                                                                                                                              >  2:2243084/1‑147 (MQ=255)
                                gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAGC‑GGGGTGCGCC‑CCCCTaa                                                                                                                             >  1:3681270/1‑150 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCTGCAGC‑GGGGTGCGCT‑CCCTTAAtat                                                                                                                          >  1:1602741/1‑148 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTCTGCCGC‑GGGGTGCGCT‑CCCCTAAttt                                                                                                                          >  2:3772615/1‑148 (MQ=255)
                                    aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTTAtgtg                                                                                                                         >  1:144837/1‑150 (MQ=255)
                                    aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAAtgtg                                                                                                                         <  1:3243462/150‑1 (MQ=255)
                                    aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAAtgtg                                                                                                                         <  1:2211217/150‑1 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCAGCAGC‑GGGGTTCGCT‑CCCCTAATGTGc                                                                                                                        >  1:1522886/1‑150 (MQ=255)
                                       gcgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCt                                                                                                                      <  2:1466985/150‑1 (MQ=255)
                                        cgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTTAGCAGC‑GGGGTGCGCT‑CCCTTTATTTGGCTc                                                                                                                     >  1:2658888/1‑150 (MQ=255)
                                         gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAGC‑GGGGTGCGCC‑CCCCTAAAGTGccccc                                                                                                                    >  1:59951/1‑147 (MQ=255)
                                             tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCat                                                                                                                <  1:2248888/150‑1 (MQ=255)
                                              cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCTTTATGTGCCTCGCCata                                                                                                               >  1:2173540/1‑150 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTAATATGCCCCGCCCTA‑T‑aa                                                                                                          >  1:1348767/1‑150 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑aa                                                                                                          <  2:485522/150‑1 (MQ=255)
                                                  ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTAATGTGCCTCGCCCTA‑T‑aaa                                                                                                         >  1:3595245/1‑150 (MQ=255)
                                                    cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAAtt                                                                                                       <  2:1699236/150‑1 (MQ=255)
                                                     gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCATGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTg                                                                                                      <  2:3842906/150‑1 (MQ=255)
                                                     gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTAATTTGCCCCCCCCTA‑T‑AAAttt                                                                                                      >  1:1734572/1‑149 (MQ=255)
                                                        gCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGGTTTTGCCGC‑GGGGTGGGCC‑CCCCTTATGTGCCTCTCCCAA‑T‑AAATTTaaa                                                                                                   >  1:2410754/1‑149 (MQ=255)
                                                        gCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑t                                                                                                  <  2:3985025/150‑1 (MQ=255)
                                                         cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGTGTTCAGCAGC‑GGGGTGGGCT‑CCCCTTATTTGGCTCCCCCTA‑T‑AAAtattatg                                                                                                  >  2:2114215/1‑144 (MQ=255)
                                                          aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTTAGCAGC‑GGGTTGCGCT‑CCCCTAATGTGCCTCGCCCTA‑T‑AAAATTAA‑Tgg                                                                                                >  1:603310/1‑150 (MQ=255)
                                                          aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTTATGTGGCTCGCCCTA‑T‑AAATTGAA‑tag                                                                                                >  1:3271954/1‑148 (MQ=255)
                                                           aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGt                                                                                               <  1:1360630/150‑1 (MQ=255)
                                                                   ttGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAg                                                                                       <  1:3045094/150‑1 (MQ=255)
                                                                             cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGGTTCTGCAGC‑GGGGTGGGGC‑CCCCTTATTTGCCCCCCCCTT‑T‑AAAATGTA‑TGGGGCCGGGGGCGCGCC‑gggg                                                                            >  2:2251866/1‑150 (MQ=255)
                                                                                 gcgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCCGCAGC‑GGGGTGCGCT‑CCCCTAATATGCCCCCCCCTA‑T‑AAATTTAA‑TGGTGGAGGGGGGGCGCC‑gggggggg                                                                        >  2:3649217/1‑150 (MQ=255)
                                                                                   gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCCTTATGTGCCTCCCCCTA‑T‑AAATTGAA‑TGGTGCAGGGGGCGCGCA‑gggggggggc                                                                      >  2:2354473/1‑148 (MQ=255)
                                                                                      cTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTTATTTGCCTCCCCCTA‑T‑AAATTTTA‑TGGGGGAGGGGGGGCGCC‑gggggggggccaa                                                                   >  2:15339/1‑145 (MQ=255)
                                                                                        aTCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATcg                                                                 <  1:801405/150‑1 (MQ=255)
                                                                                         tCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTTCGCAGC‑GGGGTGCGCT‑CCCTTTATTTGGCCCCCCCTA‑T‑AAATTTAA‑TGGGGCAGGGGGCGCGCC‑GGGGGGGGCCCAAcgc                                                                >  2:1352212/1‑150 (MQ=255)
                                                                                          cATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGTTTTGGC‑GC‑GGGGTGTGGT‑CCCCTTATCTGGCCCCCCATA‑T‑ATAATTAA‑AGGGGGACGGGGCGCGGACGGGGGGGGCCATTccc                                                                >  2:2762554/1‑148 (MQ=255)
                                                                                          cATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGGTTCAGCAGC‑GGGGTGGGCT‑CCCTTTTTGTGTCCCCCCCCA‑TAAAATTGAA‑‑GGTGGAGGGGGGGGCCC‑GGGGGCGGGCCAAcgcc                                                               >  2:4002272/1‑150 (MQ=255)
                                                                                              cGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTTCGCCAC‑GGGGTTCGCTCCCCTTAAT‑TGCCCCTCCCTA‑T‑AAATTTGA‑TGGTGCGGGGGGCGCCCC‑GGGGGCGGCCACTagcccccg                                                           >  2:436407/1‑150 (MQ=255)
                                                                                                   ccgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTCCGCAGC‑GGGGTGGCCC‑CCCCTTATGTGCCCCCCCCCA‑T‑AAAATTTA‑AGGGGGAGGGGGGGCGGG‑GGGGGGGGCCAAACGCCGcccccccc                                                      >  2:2449211/1‑150 (MQ=255)
                                                                                                    cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTTTGCAGCGGGGTGGGGCC‑CCCTTATTTTGCC‑CGCCCCATT‑AAATTAAA‑T‑GTGGAGGGGGGGGGCA‑GGGGGGGGGCCAccccccccccccccc                                                     >  2:2308587/1‑149 (MQ=255)
                                                                                                     gccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAGC‑GGGGTGCCCC‑CCCCTTATGTGCCCCGCCCTC‑T‑AAATTTAA‑TGGGGCGGGGGGGGCGCC‑CGGGGGGGCCAAACACCCCCGccccccg                                                    >  2:519307/1‑148 (MQ=255)
                                                                                                       cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCGCAGC‑GGGGTGCGCT‑CCCCTTATGTGCCTCCCCCTA‑T‑TAATTTAA‑TGGGGGAGGGGGGGGGCA‑GGGGGGGGCCAAACGCCGCCGCCCCccg‑ct                                                 >  2:2663490/1‑150 (MQ=255)
                                                                                                       cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCAGCAGC‑GGGGTGCGCT‑CCCCTAATGTGGCTCCCCCTA‑T‑AAATTTAA‑TGGTGCAGGGGGGGCGCC‑GGGGGGGGGCCATCGCCGCCCCCCCccg‑ct                                                 >  2:2404105/1‑150 (MQ=255)
                                                                                                       cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCCGCAGC‑GGGGTGCGCC‑CCCCTTATGTGCCCCCCCCTA‑T‑AAATTTAA‑TGGGGCAGGGGGCGCGCC‑GGGGGCGGCCCAACGCcccccccccccccc                                                  >  2:1437658/1‑146 (MQ=255)
                                                                                                       cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTTATGTGCCTCGCCCTA‑T‑AAATTTGA‑TGGTGCAGGGGGCGCGCC‑GGGGGGGGGCACTCGCCGCCGcccccccct                                                  >  2:1403409/1‑146 (MQ=255)
                                                                                                       cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGCTTCCGCAGC‑GGGGTGCGCT‑CCCCTAATGTGCCCCGCCCTC‑T‑AAATTTAA‑TTGGGCAGGGGGGGCGCA‑CGGGGGGGCCCAACGCCGCCGccccccccc                                                  >  2:2119563/1‑146 (MQ=255)
                                                                                                           aCGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCGCAGC‑GGGGTGGGCC‑CCCCTTATGTGCCTCGCCCTA‑T‑AAATTTAA‑TGGGGCAGGGGGCGGGCA‑GGGGGGGGCCCATCCCCCCCCCCCCCCC‑CT‑GTcc                                            >  2:3280647/1‑150 (MQ=255)
                                                                                                              gTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGGTTTGCGAGC‑GGGGGGGGCT‑CCCCTTCTGTTTCCCGCCCTA‑T‑AAATTTGA‑TGGTGGTGGGGGCGGCCA‑TGGGGCGGCCCAACTCCCCCCCCCCCCC‑CT‑GTCCCgg                                         >  2:2807791/1‑150 (MQ=255)
                                                                                                                 gCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCt                                      <  2:2688770/150‑1 (MQ=255)
                                                                                                                     gAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCGCAGC‑GGGGTGCGCC‑CCCCTAATGTGCCTCGCCCCA‑T‑AAATTTAA‑GAGTGGAGGGGGCGCCCG‑GGGGGCGGCCCAACACCCCCGCCCCCCC‑CC‑GTCCCCGCCCTCgg                                  >  2:1328286/1‑150 (MQ=255)
                                                                                                                      aaaGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCGCAGC‑GGGGTGGGCT‑CCCCTTATTTGGCTCGCCCTA‑T‑AAATTTAATTGGGGCGGGGGGGCGGCG‑GGGGGGGGCCAA‑CGCCCCCGCCCCCCGCCT‑GTCCCGCCCTTCgg                                  >  2:1779505/1‑150 (MQ=255)
                                                                                                                        aGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGa                               <  2:2051752/150‑1 (MQ=255)
                                                                                                                         gCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCCGCCGC‑CGGGTGCGCC‑CCCCTCATGTGCCCCCCCCCA‑T‑TAATTTTA‑TTGTGCAGGGGGCCCCCC‑GGGGGGGGCCCATCGCCCCCCCCCCCCC‑CC‑GTCCCCGCCCTCcgggga                              >  2:684764/1‑148 (MQ=255)
                                                                                                                            aaGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAGC‑GGGGTGCGCT‑CCCCTTATGTGGCTCGCCCTT‑T‑AAATTTAA‑TGGGGCGGGGGGGGGGCG‑GGGGGGGGCCCAACGCCCCCGCCCCCCG‑CTCGCCCCGGCCTCGGGGGaagg                            >  2:1123544/1‑148 (MQ=255)
                                                                                                                                gACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCa                       <  1:3197815/150‑1 (MQ=255)
                                                                                                                                gACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTGCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCa                       <  2:3517808/150‑1 (MQ=255)
                                                                                                                                      gCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGAtt                 <  2:1049778/150‑1 (MQ=255)
                                                                                                                                        cTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGAtttt               <  1:97873/150‑1 (MQ=255)
                                                                                                                                           cggc‑gg‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGAc            <  1:2732899/150‑1 (MQ=255)
                                                                                                                                            ggc‑gg‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGGGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACg           <  2:648767/150‑1 (MQ=255)
                                                                                                                                             gc‑gg‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGc          >  1:2443370/1‑150 (MQ=255)
                                                                                                                                              c‑gg‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcc         >  1:102455/1‑150 (MQ=255)
                                                                                                                                                 g‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac       >  2:1985583/1‑150 (MQ=255)
                                                                                                                                                 g‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac       >  1:2419695/1‑150 (MQ=255)
                                                                                                                                                    tttgtttgGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     >  1:415803/1‑150 (MQ=255)
                                                                                                                                                     ttgtttgGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCaa    >  1:242047/1‑150 (MQ=255)
                                                                                                                                                      tgtttgGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAac   >  2:1221786/1‑150 (MQ=255)
                                                                                                                                                       gtttgGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  <  1:269616/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                             
GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCT‑CCCTTAATGTGCCTCGCCATA‑T‑AAATTGAA‑TGGTGCAGGGAGCGCGCA‑GGGGGCGGCCAATCGCCGCCGCCCCCTG‑CT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑