Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,421,1470TG24.5% 420.7 / 10.2 207T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (91/63);  new base G (50/0);  total (143/64)
Fisher's exact test for biased strand distribution p-value = 6.16e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 263 total aligned reads displayed.

TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGA  >  AP012306/1420998‑1421295
                                                                                                                                                     |                                                                                                                                                    
  ccTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGa                                                                                                                                                    >  2:3810471/1‑150 (MQ=255)
   cTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGAt                                                                                                                                                   >  1:3008788/1‑150 (MQ=255)
    tGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATc                                                                                                                                                  >  1:3270183/1‑150 (MQ=255)
    tGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATc                                                                                                                                                  <  2:1704228/150‑1 (MQ=255)
    tGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATc                                                                                                                                                  >  1:1825756/1‑150 (MQ=255)
       aaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGc                                                                                                                                               <  1:2946515/150‑1 (MQ=255)
        aGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCa                                                                                                                                              >  2:2305489/1‑150 (MQ=255)
        aGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTTCa                                                                                                                                              >  2:1926776/1‑150 (MQ=255)
         gACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGcct                                                                                                                                             >  2:3040858/1‑148 (MQ=255)
         gACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAt                                                                                                                                             >  1:2890091/1‑150 (MQ=255)
          aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCAtt                                                                                                                                            >  1:2151987/1‑150 (MQ=255)
          aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                                                                                                                            <  1:2768343/150‑1 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCAtttt                                                                                                                                          >  2:2868364/1‑150 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAtttt                                                                                                                                          >  2:2691850/1‑150 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttt                                                                                                                                         >  2:695214/1‑150 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCAttttt                                                                                                                                         >  1:201327/1‑150 (MQ=255)
              gaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtttttt                                                                                                                                        <  1:854546/150‑1 (MQ=255)
               aagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCAttttttt                                                                                                                                       >  2:512482/1‑150 (MQ=255)
                 gTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCttttttttg                                                                                                                                     >  2:3437191/1‑148 (MQ=255)
                  ttCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGac                                                                                                                                    <  2:1139004/150‑1 (MQ=255)
                   tCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTaca                                                                                                                                   >  2:1829332/1‑150 (MQ=255)
                      aCATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTacaaca                                                                                                                                >  1:932704/1‑150 (MQ=255)
                          catcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAgc                                                                                                                            <  1:1012973/150‑1 (MQ=255)
                           atcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCCTTTTTTTGACAACAGAgcg                                                                                                                           >  1:109582/1‑150 (MQ=255)
                             caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGcc                                                                                                                         >  1:3310202/1‑150 (MQ=255)
                              aaaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAAAAGAGCGccc                                                                                                                        >  2:1209858/1‑150 (MQ=255)
                                 aTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCcttc                                                                                                                     >  1:2312428/1‑150 (MQ=255)
                                 aTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCcttc                                                                                                                     <  1:2846002/150‑1 (MQ=255)
                                   ggCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTTCAACAGAGCGCCcttctt                                                                                                                   >  2:1611924/1‑150 (MQ=255)
                                    gCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCttt                                                                                                                  >  2:3207106/1‑150 (MQ=255)
                                     cGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTg                                                                                                                 >  1:1430835/1‑150 (MQ=255)
                                        tAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTgaga                                                                                                              <  2:1015943/150‑1 (MQ=255)
                                        tAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTgaga                                                                                                              <  2:2966510/150‑1 (MQ=255)
                                        tAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTgaga                                                                                                              >  1:2099450/1‑150 (MQ=255)
                                         aaaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGGATTTTTTTTTCAACAGAGCGCCCTTCttttataa                                                                                                             >  2:360189/1‑145 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAGAGCGCCCCTCTTTTAGaaa                                                                                                            >  2:1276088/1‑150 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGaaa                                                                                                            >  1:549691/1‑150 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTTACAACAGAGCGCCCTTCTTTGAGaaa                                                                                                            >  2:223174/1‑150 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGaaa                                                                                                            <  1:3896161/150‑1 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTTCCTTTTTTTGACAACAAAGGGCCCTTCTTTTAGaaa                                                                                                            >  2:278757/1‑150 (MQ=255)
                                           aaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGATGATCTGCCATTTTTTTACAACCGACGGCCCCTCTTTGCGAAAc                                                                                                           >  1:4009379/1‑150 (MQ=255)
                                            aaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTTTCAACAGAGCGCCCTTCTTTTGGAAAcc                                                                                                          >  2:3896102/1‑150 (MQ=255)
                                             aTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCa                                                                                                         >  1:413471/1‑150 (MQ=255)
                                                aaCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGc                                                                                                      <  1:3489496/150‑1 (MQ=255)
                                                aaCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGTGTCTGCCATTTTTTTATAAAAAAACGCCCCTCTTTTTGAAAACACGc                                                                                                      >  2:2932656/1‑150 (MQ=255)
                                                 aCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCt                                                                                                     >  1:2798990/1‑150 (MQ=255)
                                                  cGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTc                                                                                                    <  1:601980/150‑1 (MQ=255)
                                                   gATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTTCATTTTTTTTGCAACAGAGCGCCCTTCTTTTTGAAAACACGcccc                                                                                                   >  2:3538606/1‑150 (MQ=255)
                                                   gATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTGACAACACAGCGCCCTTCTTTGAGAAACCACGCTcc                                                                                                   >  1:3062140/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCca                                                                                                  <  1:779045/150‑1 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCcag                                                                                                 <  1:1649380/150‑1 (MQ=255)
                                                       atcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTGCAAAAGAGCGCCCTTCTTTTAGAAAACACGCTcctacc                                                                                               >  2:2738647/1‑146 (MQ=255)
                                                       atcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCcagca                                                                                               >  2:2486680/1‑150 (MQ=255)
                                                         cTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAAAAAAGCGCCCTTCTTTGAGAAACCACGCTCCagcagc                                                                                             >  2:138240/1‑150 (MQ=255)
                                                            ccGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTTACAACAGAGCGCCCTTCTTTTGGAAACCACGCTCCAGCAGCGGt                                                                                          >  2:786617/1‑150 (MQ=255)
                                                             cGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCCGCATTTTTTTTTCAAAAGAACCCCCTTCTTTTAGAAAACACACCCCCCCAGCGGTc                                                                                         >  2:1792160/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCca                                                                                  >  1:2478700/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACACAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGGCAGCTCca                                                                                  >  2:3291147/1‑150 (MQ=255)
                                                                     ttCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAAACACGCTCCAGCAGCGGTCAGCTCcac                                                                                 >  1:385387/1‑150 (MQ=255)
                                                                      tCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCcaca                                                                                <  1:2812818/150‑1 (MQ=255)
                                                                      tCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGCGATCTGCATTTTTTTGACAAAAGAACGCCCTTCCTTTTGAAAACACGCTCCAGCAGCGGTCAGCtgccca                                                                                >  2:2123602/1‑145 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACCCTCCAGCAGCGGTCAGCTCCACAt                                                                               >  1:1225056/1‑150 (MQ=255)
                                                                        tGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATc                                                                              <  2:528803/150‑1 (MQ=255)
                                                                        tGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCTTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGTCAGCTCCCCATc                                                                              >  2:567302/1‑150 (MQ=255)
                                                                        tGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCCGCGGGCAGCTCCACATc                                                                              >  2:3146329/1‑150 (MQ=255)
                                                                        tGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCCTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATc                                                                              >  1:3140188/1‑150 (MQ=255)
                                                                           ccTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACg                                                                           >  1:632067/1‑150 (MQ=255)
                                                                              gACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTTCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCCGCGGGCAGCTCCCCATCACCGTg                                                                        >  2:1352548/1‑150 (MQ=255)
                                                                              gACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTg                                                                        >  1:1190402/1‑150 (MQ=255)
                                                                              gACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTg                                                                        >  1:3918806/1‑150 (MQ=255)
                                                                                  atatTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgt                                                                    >  2:1441110/1‑150 (MQ=255)
                                                                                      tatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGtt                                                                >  1:368443/1‑150 (MQ=255)
                                                                                       atCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTc                                                               >  1:486167/1‑150 (MQ=255)
                                                                                        tCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAAAGCGCCCCTCTTTGAGGAACCACCCCCCAGCAGCGGGCAGCCCCACATCACCGTGCCGTCGTTCa                                                              >  2:3313450/1‑150 (MQ=255)
                                                                                         ccACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCaa                                                             <  2:3780475/150‑1 (MQ=255)
                                                                                           aCTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAag                                                           <  1:1953412/150‑1 (MQ=255)
                                                                                            cTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAaga                                                          <  1:3649740/150‑1 (MQ=255)
                                                                                            cTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAaga                                                          <  1:2410387/150‑1 (MQ=255)
                                                                                             tGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAagag                                                         >  1:3159680/1‑150 (MQ=255)
                                                                                               cGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCg                                                       >  1:3186111/1‑150 (MQ=255)
                                                                                                gATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCCTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCCCCACATCACCGTGCCGTCGTTCAAAGAGCgg                                                      >  2:2030176/1‑150 (MQ=255)
                                                                                                   aGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAc                                                   <  1:237317/150‑1 (MQ=255)
                                                                                                    gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAcc                                                  >  1:1326684/1‑150 (MQ=255)
                                                                                                    gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAcc                                                  <  1:1257168/150‑1 (MQ=255)
                                                                                                       aaCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgcc                                               <  1:1802760/150‑1 (MQ=255)
                                                                                                        aCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgcca                                              >  1:16222/1‑150 (MQ=255)
                                                                                                             ccGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGGGAAACCACGCCCCCGCAGCGGGCAGCCCCACATCACGGTGCCGTCGTTCAAAAAGCGGCCCCgccagcccc                                         >  2:414451/1‑148 (MQ=255)
                                                                                                                  ccAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAGAGCGCCCCTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAAAGCGGCCCCCCCAGCCACTCTTc                                    >  2:1981268/1‑150 (MQ=255)
                                                                                                                   cAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTcc                                   >  1:1556074/1‑150 (MQ=255)
                                                                                                                    aGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCa                                  >  1:3395150/1‑150 (MQ=255)
                                                                                                                      ccGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCCCCAGCCACTCTTCCAGc                                >  1:3469817/1‑150 (MQ=255)
                                                                                                                           cGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCCGCTGAcc                           >  2:3882095/1‑149 (MQ=255)
                                                                                                                                 ctgctgACATTGCTGGCGGGGGGTCTGCCTTTTTTTTACAACAGAGCGCCCTTCTTTGTGAAAACCCCCTCCAGCAGCGGTCAGCCTCCCATCACGGTGCCCCCGTTATAAGAGCGGCACCCCCCGCCACTCTTCCACCTTGCGCACggg                     >  2:1574243/1‑149 (MQ=255)
                                                                                                                                     tgACATTGCTGGCGGGGGATCTGCCTTTTTTTTACAACAGAGCGCCCTTCTTTTTGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTTAAAGAGCGGCACCGCCAGCCCCTCTTCCCGCCGACGCCCCGTATAg                 >  2:2547862/1‑150 (MQ=255)
                                                                                                                                       aCATTGCTGGCGGGGGATCTGCATTTTTTTTACAAAAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCCCGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCCCTCTTCCCGCTGACCCACCGGATAGCt               >  2:2158546/1‑150 (MQ=255)
                                                                                                                                        cATTGCTGGCGGGGGATTTGCCTTTTTTTTACAACAAAGCGCCCTTTTTTTGGAAACCACACTCCAGCAGCGGTGAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCCCCAACCACCCTTCCAGCTGACGCCCGGTATACCTg              >  2:650722/1‑150 (MQ=255)
                                                                                                                                            gCTGGCGGGGGGTCTTCATTTTTTTTGCAACAAAGCGCCCTTCTTTGAGAAACCACCCCCCCGCCGCGGTCCGCTCCCCATCCCGGTGCCGTCGTTTCAAAAGCGGCCCCCCCCGCCCCCCTTTCCGCTGACGCCCGGGATAAGTGACCg          >  2:1532762/1‑150 (MQ=255)
                                                                                                                                              tGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACAACACGGGGCCGTCGTTCAAAGAGCGGCACCGCCCGCCACCCTTCCAGCTGACGCACGGGATAGCTGACCGCa        >  2:1909145/1‑150 (MQ=255)
                                                                                                                                                   ggTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAgg   >  1:2679792/1‑150 (MQ=255)
                                                                                                                                                    gTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGa  >  2:3723429/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGA  >  AP012306/1420998‑1421295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑