Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,560,2430TG24.6% 421.2 / 22.3 171N419T (AAC→ACC) pepPproline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (38/91);  new base G (42/0);  total (80/91)
Fisher's exact test for biased strand distribution p-value = 5.49e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 192 total aligned reads displayed.

CAGCGCCCCGTGACTTAACCGGG‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCC  >  AP012306/2560096‑2560392
                                                                                                                                                     |                                                                                                                                                     
aaGCGCCCCGTGAC‑TAACCGGGAAAATGG‑CCAGCCCAGGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGttt                                                                                                                                                     <  2:1390746/149‑1 (MQ=255)
 aGCGCCCCGTGACTTAACCGGG‑AAATAG‑CCAGCGCCAGCGCCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGtttt                                                                                                                                                    <  2:2075923/150‑1 (MQ=255)
  gcgcCCCGTGACTTAACCGGG‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTc                                                                                                                                                   <  1:983304/150‑1 (MQ=255)
      cccGTGACTTACCCGGA‑AAATAG‑CCAGCCCCGGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTa                                                                                                                                               <  2:2508622/150‑1 (MQ=255)
      cccGTGACTTAACCGGG‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTa                                                                                                                                               <  1:1761804/150‑1 (MQ=255)
       ccGTGACTTAACCGGG‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTAc                                                                                                                                              >  1:1696622/1‑150 (MQ=255)
          tGACTTAACCGGG‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCgg                                                                                                                                           >  1:1636832/1‑150 (MQ=255)
             cTTACCCGGG‑AAATAG‑CCAGCGCCAGCGTCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGttt                                                                                                                                        <  2:1376273/150‑1 (MQ=255)
              ttAACCGGG‑AAAAGGCCCGGCCCCGGCGCGGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGttt                                                                                                                                        <  2:2931775/150‑1 (MQ=255)
                aaCCGGG‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCgg                                                                                                                                     >  2:661048/1‑150 (MQ=255)
                      g‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATca                                                                                                                               >  1:2563953/1‑150 (MQ=255)
                       aaaaTAG‑CCGGCCCCGGCGCCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATca                                                                                                                               <  2:281078/149‑1 (MQ=255)
                        aaaTAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCCGTTTCGGTAATcac                                                                                                                              >  2:3972545/1‑150 (MQ=255)
                        aaaTAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGCTTCGGTAATcac                                                                                                                              >  2:2774626/1‑150 (MQ=255)
                             g‑ccagcgccagcGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgt                                                                                                                         >  1:2744895/1‑150 (MQ=255)
                                     ccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAAAGTCGTCTTc                                                                                                                  >  1:1241088/1‑150 (MQ=255)
                                       agcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCaa                                                                                                                >  1:1842612/1‑150 (MQ=255)
                                        gcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAAt                                                                                                               <  1:1392518/150‑1 (MQ=255)
                                          gccgcgCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATAc                                                                                                             <  2:1520408/148‑1 (MQ=255)
                                          gTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATAc                                                                                                             <  2:838792/150‑1 (MQ=255)
                                          gTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATAc                                                                                                             >  2:3548528/1‑150 (MQ=255)
                                           tCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACg                                                                                                            <  1:1018659/150‑1 (MQ=255)
                                               gcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTTGTTATCGGTTTCCGTAATCACCATGTCGTCTTCAATACGAAta                                                                                                        >  2:2122624/1‑149 (MQ=255)
                                                cccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGc                                                                                                       >  2:1315231/1‑150 (MQ=255)
                                                 ccgccATGCCGGCAACGACGATGATTGCGCTCATTGCTTTCTCGCAGCAACCATCAACGCGTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTGCCGGTTTCGGTTATCACAATGGCGTCTTCAATACGAATGcc                                                                                                      <  2:2099576/150‑1 (MQ=255)
                                                 ccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGcc                                                                                                      >  2:1356174/1‑150 (MQ=255)
                                                  cgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCg                                                                                                     <  2:2255585/150‑1 (MQ=255)
                                                    ccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGAt                                                                                                   <  1:2516878/150‑1 (MQ=255)
                                                        gccgccACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACcg                                                                                               <  1:2697855/150‑1 (MQ=255)
                                                        gccgccACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGGTTCGGTAATCACAATGTCGGCTTCAATACGAATGCCGATACcg                                                                                               >  1:3153946/1‑150 (MQ=255)
                                                         ccgccACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACcgc                                                                                              <  1:2834149/150‑1 (MQ=255)
                                                             cACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGata                                                                                          <  2:3655530/150‑1 (MQ=255)
                                                             cACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGata                                                                                          >  1:3354703/1‑150 (MQ=255)
                                                              aCCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGatat                                                                                         <  2:3596207/150‑1 (MQ=255)
                                                               ccGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATAtt                                                                                        >  1:3007652/1‑150 (MQ=255)
                                                               ccGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATAtt                                                                                        <  1:1508568/150‑1 (MQ=255)
                                                                    gatgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCt                                                                                   <  2:2475550/150‑1 (MQ=255)
                                                                    gatgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCt                                                                                   >  2:3354709/1‑150 (MQ=255)
                                                                     atgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTg                                                                                  <  2:494755/150‑1 (MQ=255)
                                                                      tgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCttg                                                                                 >  1:1878767/1‑148 (MQ=255)
                                                                      tgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCttg                                                                                 >  1:1738349/1‑148 (MQ=255)
                                                                       gatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGc                                                                                >  2:2261348/1‑150 (MQ=255)
                                                                        atTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCa                                                                               >  2:2025673/1‑150 (MQ=255)
                                                                            cGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTc                                                                           >  1:3745710/1‑150 (MQ=255)
                                                                             gCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCt                                                                          <  1:3122568/150‑1 (MQ=255)
                                                                             gCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCt                                                                          <  1:1960691/150‑1 (MQ=255)
                                                                              cTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCCCGATATTGTTCTGGCACTTCTg                                                                         >  1:3551939/1‑150 (MQ=255)
                                                                               tCATTGCTTTCTCGCAGCAACCATCAACGCTTCTATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGc                                                                        <  2:1456840/150‑1 (MQ=255)
                                                                               tCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGc                                                                        <  1:3915943/150‑1 (MQ=255)
                                                                                cATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCa                                                                       >  1:2718600/1‑150 (MQ=255)
                                                                                   tGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATcc                                                                    <  1:3865390/150‑1 (MQ=255)
                                                                                    gCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGGTATTGTTCTGGCACTTCTGCATCCg                                                                   >  2:1994848/1‑150 (MQ=255)
                                                                                      tttCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgc                                                                 <  2:2721593/150‑1 (MQ=255)
                                                                                      tttCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTTGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCCCGGTAATTTTTTTGCCCTTCTTCATCCCgc                                                                 >  2:4048956/1‑150 (MQ=255)
                                                                                         ctcGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCCGCGCa                                                              >  1:3053321/1‑150 (MQ=255)
                                                                                            gcagcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAata                                                           <  1:713249/150‑1 (MQ=255)
                                                                                             cagcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGAGGTTTTCGGTACCGGTTTCTGTAATCACAATGTCGGCTTCAATACGAATGCCGATACCGCGATATTTTTCTTGCACTTCTTCATTCGGCGCAatat                                                          >  2:2699031/1‑150 (MQ=255)
                                                                                              agcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAAtata                                                         <  1:634138/150‑1 (MQ=255)
                                                                                              agcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAAtata                                                         >  2:3230817/1‑150 (MQ=255)
                                                                                               gcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGGGGGTTTCGGTACCGGGTTCGGTAATCACAAAGTCTTCTTTGATACGAATTCCCATACCGCGCGATTGTTCTTGCACTTCTGCATGCGGCGCAATATAc                                                        >  2:157480/1‑150 (MQ=255)
                                                                                                 aaCCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAg                                                      >  1:2925170/1‑150 (MQ=255)
                                                                                                   ccATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGcc                                                    <  2:1616991/150‑1 (MQ=255)
                                                                                                     aTCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCt                                                  <  1:3487835/150‑1 (MQ=255)
                                                                                                      tCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTTGCACTTCTGCATCCGGCGCAATATACAGCCCTg                                                 >  2:1211324/1‑150 (MQ=255)
                                                                                                       cAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTgg                                                >  1:3257815/1‑150 (MQ=255)
                                                                                                       cAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCCGCGCAATATACAGCCCTgg                                                >  2:1647291/1‑150 (MQ=255)
                                                                                                           gCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTTGTTTCCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTTTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct                                            >  1:807057/1‑150 (MQ=255)
                                                                                                            cTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGGGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGGTACCGCGATATTTTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTa                                           >  2:110765/1‑150 (MQ=255)
                                                                                                             ttCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTat                                          <  1:939611/150‑2 (MQ=255)
                                                                                                             ttCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTTCCCGTTTTGGTAATCACCATGTCGTCTTCAATACGAATGCCGATACCGCGGTATTGTTCTGGCACTTCTGCATCCCGCGCAATATACAGCCCTGGCTCTAc                                          >  2:1830439/1‑150 (MQ=255)
                                                                                                              tCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACg                                         <  1:1536849/150‑1 (MQ=255)
                                                                                                              tCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTTGTTACCCGGTTCGGTAATCACAATATCGTCTTTCATACGAAAGCCGGTACCGCGGTATTTTTCTGGCACTTCCGCCTCCGGCGCCATATACCGCCCCCGctccccg                                         >  2:967004/1‑146 (MQ=255)
                                                                                                              tCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTTCCCGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGGTATTGTTCTTGCACTTCTTCATCCGGCGCAATATACAGCCCTGGCTCTACg                                         >  2:1246099/1‑150 (MQ=255)
                                                                                                               cGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGGTACCGCGGTATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACgg                                        >  2:2242465/1‑150 (MQ=255)
                                                                                                               cGATTTCTTCAGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACgg                                        <  1:2383782/150‑1 (MQ=255)
                                                                                                                gATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGt                                       <  1:1433810/150‑1 (MQ=255)
                                                                                                                 aTTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTc                                      >  1:1987987/1‑150 (MQ=255)
                                                                                                                  tttCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCa                                     <  2:1924701/150‑1 (MQ=255)
                                                                                                                        ccGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCa                               <  1:2532664/150‑1 (MQ=255)
                                                                                                                         cGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTTGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCAt                              >  1:3052399/1‑150 (MQ=255)
                                                                                                                          ggCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATg                             >  1:1985782/1‑150 (MQ=255)
                                                                                                                          ggCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTTGGTAATCACAAAGTCGGCCTCAATACGAATTCCCATACCGCGGTTTTGTTCTGTCACTTCTCCATCCCGCGCGATATACAGCCCTCGCTCTACGGGCAGTACCCTg                             >  2:1427676/1‑150 (MQ=255)
                                                                                                                          ggCTTTTTCACCACGCTGGCGGTGAGGGTTTTGTTACCCGGTTCGGTAATCACAATGCCGTCTTTAATACGAATGCCGCAACCGCGTTATTGTTCTGGCACTTCTGCCTCCCGCGCAATATAAAGCCCCGGCTTTTCGGGCAAAACCaag                             >  2:2385714/1‑148 (MQ=255)
                                                                                                                          ggCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCCGTTTCGGTAATCACAATATCGTCTTCAATACGAATGCCGATACCGCGATATTGGTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCCGGCTCTACCGTCAGGACCATg                             >  2:3898809/1‑150 (MQ=255)
                                                                                                                           gCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAAAGCCGATACCGCGGTATTGTTTTGGCACTTCTGCATCCGGCGCAATATAAAGCCCCGGCCCTACGGTCAGTACCCTGc                            >  2:2968259/1‑150 (MQ=255)
                                                                                                                             tttttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGccc                          >  2:579381/1‑150 (MQ=255)
                                                                                                                               tttCACCACGCTGGCGGTGAGGTTTTTGTTACCGGGTTCGGAAATCACAAAGTCGGCTTAAATACGAATGACGATACCGCGATATTTTTCTTTCAATTCTTCATCCCGCGCAAAATACAGCCCCGTCTCTTCGGTCACTAACATGccccg                        >  2:219863/1‑148 (MQ=255)
                                                                                                                                  caccacGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTc                     >  1:1180175/1‑150 (MQ=255)
                                                                                                                                      acGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGa                 <  2:772306/150‑1 (MQ=255)
                                                                                                                                        gCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAAt               <  2:3128370/150‑1 (MQ=255)
                                                                                                                                            gCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATgcgc           <  1:561471/150‑1 (MQ=255)
                                                                                                                                             cGGTGGGGGTTTCGTTACTGGATTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGcgcg          >  2:929489/1‑150 (MQ=255)
                                                                                                                                                tGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagc       >  1:2640809/1‑150 (MQ=255)
                                                                                                                                                tGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagc       >  1:2179136/1‑150 (MQ=255)
                                                                                                                                                 gAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagcg      >  2:1081018/1‑150 (MQ=255)
                                                                                                                                                 gAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagcg      >  2:1327450/1‑150 (MQ=255)
                                                                                                                                                 gAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagcg      >  1:1074199/1‑150 (MQ=255)
                                                                                                                                                   ggTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGAt    >  1:442928/1‑150 (MQ=255)
                                                                                                                                                    gTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATc   <  1:377777/150‑1 (MQ=255)
                                                                                                                                                    gTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATc   >  2:2744659/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCGCCCCGTGACTTAACCGGG‑AAATAG‑CCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCC  >  AP012306/2560096‑2560392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑