BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,805,972	C→A	intergenic (+171/‑5)	ecnB → / → sugE	entericidin B membrane lipoprotein/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,805,972	0	C	A	100.0%	1278.0 / NA	325	intergenic (+171/‑5)	ecnB/sugE	entericidin B membrane lipoprotein/multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (130/195); total (130/195)

Only 100 of 326 total aligned reads displayed.

TCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCT  >  AP012306/3805825‑3806118

tCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATg                                                                                                                                                  <  1:1064341/150‑1 (MQ=255)

 cGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGa                                                                                                                                                 <  1:3244781/150‑1 (MQ=255)

    gCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGatat                                                                                                                                              >  1:500988/1‑150 (MQ=255)

       gtttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTc                                                                                                                                           >  2:339997/1‑150 (MQ=255)

          tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTg                                                                                                                                        >  1:664786/1‑150 (MQ=255)

          tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTg                                                                                                                                        >  2:744821/1‑150 (MQ=255)

          tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTg                                                                                                                                        <  2:1506824/150‑1 (MQ=255)

          tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTg                                                                                                                                        <  1:3070178/150‑1 (MQ=255)

           gtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTgg                                                                                                                                       >  2:322663/1‑150 (MQ=255)

             tttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGAt                                                                                                                                     <  2:332772/150‑1 (MQ=255)

             tttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGACATGTCCTGGAt                                                                                                                                     <  2:3922939/150‑1 (MQ=255)

              ttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGAtt                                                                                                                                    >  2:3134944/1‑150 (MQ=255)

              ttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGAtt                                                                                                                                    <  1:2472237/150‑1 (MQ=255)

                ttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTAt                                                                                                                                  >  1:1202370/1‑150 (MQ=255)

                 tCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATc                                                                                                                                 >  2:306036/1‑150 (MQ=255)

                 tCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATc                                                                                                                                 <  2:1017160/150‑1 (MQ=255)

                  cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCt                                                                                                                                >  2:1387993/1‑150 (MQ=255)

                  cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCt                                                                                                                                <  1:721948/150‑1 (MQ=255)

                  cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCt                                                                                                                                >  2:2777781/1‑150 (MQ=255)

                  cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCt                                                                                                                                <  1:1545474/150‑1 (MQ=255)

                    gCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTa                                                                                                                              <  1:1706157/150‑1 (MQ=255)

                    gCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTa                                                                                                                              <  1:2737041/150‑1 (MQ=255)

                     cGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGGCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAg                                                                                                                             >  1:3633355/1‑150 (MQ=255)

                      gATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGt                                                                                                                            >  1:627482/1‑150 (MQ=255)

                       aTAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGtt                                                                                                                           <  1:971578/150‑1 (MQ=255)

                        tAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTa                                                                                                                          <  2:2049804/150‑1 (MQ=255)

                           tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTg                                                                                                                       <  1:2067737/150‑1 (MQ=255)

                           tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTg                                                                                                                       <  1:347246/150‑1 (MQ=255)

                           tCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTCATTg                                                                                                                       >  2:3470618/1‑150 (MQ=255)

                            cacaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGc                                                                                                                      <  2:1579662/150‑1 (MQ=255)

                                     aaTAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTctgct                                                                                                             >  1:332040/1‑150 (MQ=255)

                                     aaTAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTctgct                                                                                                             >  2:2156888/1‑150 (MQ=255)

                                             aaaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTgg                                                                                                     >  2:3738627/1‑150 (MQ=255)

                                                 tCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATg                                                                                                 <  1:2224233/150‑1 (MQ=255)

                                                 tCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATg                                                                                                 <  2:2617405/150‑1 (MQ=255)

                                                  ccccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                                                                                <  2:1654380/150‑1 (MQ=255)

                                                     cTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGcc                                                                                             >  2:256529/1‑150 (MQ=255)

                                                      tGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCg                                                                                            <  2:861166/150‑1 (MQ=255)

                                                       gCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGt                                                                                           >  2:1919455/1‑150 (MQ=255)

                                                       gCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGt                                                                                           <  2:6390/150‑1 (MQ=255)

                                                           ccTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGc                                                                                       <  2:301855/150‑1 (MQ=255)

                                                           ccTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGc                                                                                       >  2:1730582/1‑150 (MQ=255)

                                                             tGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCt                                                                                     <  2:2921355/150‑1 (MQ=255)

                                                             tGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCt                                                                                     <  2:2021545/150‑1 (MQ=255)

                                                              ggCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTg                                                                                    <  2:440057/150‑1 (MQ=255)

                                                                cAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaa                                                                                  <  2:2823639/150‑1 (MQ=255)

                                                                cAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaa                                                                                  >  2:2411296/1‑150 (MQ=255)

                                                                 aaaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaaa                                                                                 >  1:1850435/1‑150 (MQ=255)

                                                                 aaaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaaa                                                                                 >  2:1326093/1‑150 (MQ=255)

                                                                  aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAat                                                                                >  2:2214353/1‑150 (MQ=255)

                                                                  aaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATAATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAat                                                                                >  1:3918304/1‑150 (MQ=255)

                                                                   aaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAata                                                                               >  1:2629774/1‑150 (MQ=255)

                                                                   aaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAata                                                                               >  2:3349006/1‑150 (MQ=255)

                                                                     tatCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAAtata                                                                             <  1:26061/150‑1 (MQ=255)

                                                                       tCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAcc                                                                           >  2:2485580/1‑150 (MQ=255)

                                                                           ttCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACg                                                                       <  1:1514647/150‑1 (MQ=255)

                                                                           ttCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACg                                                                       <  2:3870591/150‑1 (MQ=255)

                                                                              aaCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCt                                                                    <  2:3967754/150‑1 (MQ=255)

                                                                              aaCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCt                                                                    <  1:2137103/150‑1 (MQ=255)

                                                                              aaCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCt                                                                    >  2:1828931/1‑150 (MQ=255)

                                                                              aaCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCt                                                                    <  1:114328/150‑1 (MQ=255)

                                                                                  aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAg                                                                >  2:54341/1‑150 (MQ=255)

                                                                                     aGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCg                                                             >  1:2797500/1‑150 (MQ=255)

                                                                                      gCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGt                                                            <  2:1621510/150‑1 (MQ=255)

                                                                                      gCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGt                                                            >  2:1867521/1‑150 (MQ=255)

                                                                                      gCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGt                                                            >  2:2598211/1‑150 (MQ=255)

                                                                                       cTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt                                                           >  1:1625644/1‑150 (MQ=255)

                                                                                        tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGttt                                                          <  2:3818338/150‑1 (MQ=255)

                                                                                           gCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGAc                                                       >  2:3582697/1‑150 (MQ=255)

                                                                                           gCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGAc                                                       <  1:1803326/150‑1 (MQ=255)

                                                                                            cAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACg                                                      <  1:2334067/150‑1 (MQ=255)

                                                                                             aGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGc                                                     >  2:3640075/1‑150 (MQ=255)

                                                                                              ggACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGcc                                                    <  1:2385109/150‑1 (MQ=255)

                                                                                                acgacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGa                                                  >  1:2950282/1‑150 (MQ=255)

                                                                                                 cgacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAg                                                 <  1:799154/150‑1 (MQ=255)

                                                                                                  gacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAgt                                                <  1:1410359/150‑1 (MQ=255)

                                                                                                  gacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAgt                                                <  1:1259652/150‑1 (MQ=255)

                                                                                                  gacCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAgt                                                <  1:3389464/150‑1 (MQ=255)

                                                                                                     cTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGtt                                             >  1:3881431/1‑150 (MQ=255)

                                                                                                       gCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGttat                                           >  1:3682591/1‑150 (MQ=255)

                                                                                                        cAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGttatt                                          >  1:4049243/1‑150 (MQ=255)

                                                                                                         aaaCGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGttatta                                         <  1:379439/150‑1 (MQ=255)

                                                                                                                tctTTTCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTTGTTTGACGCCGAGTGTTATTACTGTGAc                                  <  1:2842861/150‑1 (MQ=255)

                                                                                                                  ttttCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACgg                                <  1:174848/150‑1 (MQ=255)

                                                                                                                  ttttCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACgg                                >  2:478/1‑150 (MQ=255)

                                                                                                                  ttttCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACgg                                <  1:3437916/150‑1 (MQ=255)

                                                                                                                    ttCACCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCg                              >  2:906275/1‑150 (MQ=255)

                                                                                                                       aCCGGGGACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCgatg                           >  1:4020997/1‑150 (MQ=255)

                                                                                                                            ggACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGt                      <  2:3536954/150‑1 (MQ=255)

                                                                                                                             gACGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTc                     <  2:2303444/150‑1 (MQ=255)

                                                                                                                               cGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAg                   <  2:1104517/150‑1 (MQ=255)

                                                                                                                               cGGCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAg                   <  1:2043375/150‑1 (MQ=255)

                                                                                                                                 gCCCCAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTa                 <  1:25472/150‑1 (MQ=255)

                                                                                                                                   cccAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATg               <  1:3192649/150‑1 (MQ=255)

                                                                                                                                   cccAATTCTCCGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATg               <  1:1952791/150‑1 (MQ=255)

                                                                                                                                            ccGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACtt      >  2:3190199/1‑150 (MQ=255)

                                                                                                                                            ccGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACtt      <  2:1992121/150‑1 (MQ=255)

                                                                                                                                            ccGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACtt      >  2:1221447/1‑150 (MQ=255)

                                                                                                                                             cGGAGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTg     <  1:1006948/150‑1 (MQ=255)

                                                                                                                                                aGCATGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCt  <  1:761109/150‑1 (MQ=255)

TCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGACGCCGAGTGTTATTACTGTGACGGCGATGATTGTCAGTATGGCGCTACTTGCCT  >  AP012306/3805825‑3806118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`