Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,854,8510AC31.0% 559.9 / 94.0 258T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
Reads supporting (aligned to +/- strand):  ref base A (156/22);  new base C (0/80);  total (156/102)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 287 total aligned reads displayed.

TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGC  >  AP012306/1854702‑1854998
                                                                                                                                                      |                                                                                                                                                   
 aGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAAc                                                                                                                                                    <  1:3514223/150‑1 (MQ=255)
 aGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAAc                                                                                                                                                    >  1:3477900/1‑150 (MQ=255)
 aGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAAc                                                                                                                                                    >  2:3264570/1‑150 (MQ=255)
 aGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAAc                                                                                                                                                    >  1:4162402/1‑150 (MQ=255)
       cTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGc                                                                                                                                              >  1:3809069/1‑150 (MQ=255)
        taataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGcc                                                                                                                                             >  1:2306408/1‑150 (MQ=255)
         aataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTACCg                                                                                                                                            >  1:2135197/1‑150 (MQ=255)
          ataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGt                                                                                                                                           >  1:1426082/1‑150 (MQ=255)
           caaCACCCCGCAAGGGAAAAAAACCCATGGAATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTCCCCCCATAACAGCAAGAACCCGTGGAGGCCCCGCT‑AACCATTTTCCGCCGTCAAAAACCCCCTGCCGtt                                                                                                                                          <  2:900502/149‑1 (MQ=255)
             aCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTAt                                                                                                                                        >  1:778510/1‑150 (MQ=255)
                tCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGt                                                                                                                                     >  2:3912039/1‑150 (MQ=255)
                  ccTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGCCATAAAACCCCTCCCGTTATCGttc                                                                                                                                   >  2:960865/1‑148 (MQ=255)
                    tCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGtgctg                                                                                                                                 >  2:3522770/1‑150 (MQ=255)
                    tCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGtgctg                                                                                                                                 <  1:1342592/150‑1 (MQ=255)
                       aGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGccc                                                                                                                              >  1:695747/1‑150 (MQ=255)
                        gggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCa                                                                                                                             <  1:3668409/150‑1 (MQ=255)
                           aaaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCg                                                                                                                          >  2:2791826/1‑150 (MQ=255)
                              aaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgcg                                                                                                                       >  2:717060/1‑150 (MQ=255)
                                   ccATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACCCTTCGCCCATACCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCTTCAAAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt                                                                                                                  <  2:2058682/150‑1 (MQ=255)
                                   ccATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt                                                                                                                  >  2:2186324/1‑150 (MQ=255)
                                    cATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTa                                                                                                                 >  1:1397798/1‑150 (MQ=255)
                                     aTGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCACCCATTTT‑CGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaa                                                                                                                <  2:2476704/150‑1 (MQ=255)
                                     aTGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaa                                                                                                                <  1:1197356/150‑1 (MQ=255)
                                     aTGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCCCCCATACCAGCAAGAAGCCGTGGATGCCACGCTCACCCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaa                                                                                                                <  2:3828605/150‑1 (MQ=255)
                                        gCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCACCCATTTT‑CGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg                                                                                                             <  2:1994597/150‑1 (MQ=255)
                                          aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                           >  1:1489921/1‑150 (MQ=255)
                                            aCTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTg                                                                                                         >  2:3478674/1‑150 (MQ=255)
                                             cTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTgg                                                                                                        >  1:1097640/1‑150 (MQ=255)
                                              tATTAGCAGAATAATCTACCTACGCGAGCCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGt                                                                                                       <  1:2566156/150‑1 (MQ=255)
                                              tATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGt                                                                                                       >  1:1631911/1‑150 (MQ=255)
                                               aTTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTg                                                                                                      >  2:2909429/1‑150 (MQ=255)
                                                  aGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATa                                                                                                   <  1:285733/150‑1 (MQ=255)
                                                         taatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAAc                                                                                            >  1:2005604/1‑150 (MQ=255)
                                                           atCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTg                                                                                          >  1:2733769/1‑150 (MQ=255)
                                                             ctacctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGc                                                                                        >  2:3092394/1‑150 (MQ=255)
                                                                cctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACg                                                                                     <  1:3424592/150‑1 (MQ=255)
                                                                cctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACg                                                                                     >  1:615783/1‑150 (MQ=255)
                                                                cctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACg                                                                                     >  1:3592251/1‑150 (MQ=255)
                                                                 ctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACgg                                                                                    >  2:504920/1‑150 (MQ=255)
                                                                   acGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggct                                                                                  >  1:2915832/1‑150 (MQ=255)
                                                                    cgcgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctg                                                                                 <  1:3159340/150‑1 (MQ=255)
                                                                     gcgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctgg                                                                                <  2:1725795/150‑1 (MQ=255)
                                                                      cgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctggc                                                                               >  2:2720156/1‑150 (MQ=255)
                                                                            cATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTgg                                                                         <  1:1928640/150‑1 (MQ=255)
                                                                               gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCCGGTAAAAGCCTGGAGATAGCGTGACTCGCACGGCTGGCTCGTGGGcg                                                                      >  2:2986173/1‑150 (MQ=255)
                                                                               gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcg                                                                      >  1:1974511/1‑150 (MQ=255)
                                                                               gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcg                                                                      >  2:143017/1‑150 (MQ=255)
                                                                               gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcg                                                                      >  2:4076815/1‑150 (MQ=255)
                                                                                  ttttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgt                                                                   <  1:1060547/150‑1 (MQ=255)
                                                                                     tACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgct                                                                >  1:2347392/1‑150 (MQ=255)
                                                                                      acacTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctg                                                               <  1:2538162/150‑1 (MQ=255)
                                                                                       cacTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctgg                                                              >  1:549087/1‑150 (MQ=255)
                                                                                        acTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggt                                                             <  1:1702891/150‑1 (MQ=255)
                                                                                         cTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtg                                                            >  2:463005/1‑150 (MQ=255)
                                                                                           tCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtgct                                                          >  2:1601049/1‑150 (MQ=255)
                                                                                              cccATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGc                                                       <  2:190987/150‑1 (MQ=255)
                                                                                              cccATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGc                                                       >  2:3312776/1‑150 (MQ=255)
                                                                                               ccATATCAGCAAGAAGCGGTGGATGCCCGCCTAAACAATTTT‑CGCCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGca                                                      <  2:2686330/150‑1 (MQ=255)
                                                                                                cATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcac                                                     >  1:880875/1‑150 (MQ=255)
                                                                                                cATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcac                                                     >  2:1746445/1‑150 (MQ=255)
                                                                                                cATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCATTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcac                                                     >  2:3931084/1‑150 (MQ=255)
                                                                                                   atCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACg                                                  <  1:1570578/150‑1 (MQ=255)
                                                                                                    tCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGt                                                 >  1:1759106/1‑150 (MQ=255)
                                                                                                     cAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTAAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGtt                                                <  1:3879378/150‑1 (MQ=255)
                                                                                                     cAGCAAGAAGCCGTGGATGCCACGCCCAACCTTTTT‑CGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGtt                                                <  2:1302563/150‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCACCCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                               <  2:126535/150‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                               >  2:2851625/1‑150 (MQ=255)
                                                                                                       gCAAGAAGCCGTGGATGCCACGCTCACCCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaa                                              <  2:4040104/150‑1 (MQ=255)
                                                                                                        cAAGAGGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaaa                                             <  1:3056434/150‑1 (MQ=255)
                                                                                                        cAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaaa                                             >  1:1532925/1‑150 (MQ=255)
                                                                                                        cAAGAAGCCGTGGATGCCACGCCAAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaaa                                             <  2:415824/150‑1 (MQ=255)
                                                                                                          agaagCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGa                                           >  2:827189/1‑150 (MQ=255)
                                                                                                          agaagCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGa                                           >  1:1675789/1‑150 (MQ=255)
                                                                                                            aagCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAAc                                         <  2:600555/150‑1 (MQ=255)
                                                                                                                   ggATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                  >  1:3103876/1‑150 (MQ=255)
                                                                                                                    gATGCCCCGCTCAACCTTTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcg                                 <  2:2236556/150‑1 (MQ=255)
                                                                                                                      tGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCa                               >  1:567477/1‑150 (MQ=255)
                                                                                                                        ccACGCTCACCCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCaaa                             <  1:3392503/150‑1 (MQ=255)
                                                                                                                           cGCTGAACCATTTT‑CCTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAAcc                          <  2:2364390/150‑1 (MQ=255)
                                                                                                                           cGCTCAGCCATTTC‑ATTCGCAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAAcc                          <  1:4063073/150‑1 (MQ=255)
                                                                                                                           cGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAAcc                          >  1:2997851/1‑150 (MQ=255)
                                                                                                                            gCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCa                         >  2:3691209/1‑150 (MQ=255)
                                                                                                                              tCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATg                       <  2:1375766/150‑1 (MQ=255)
                                                                                                                               cacccATTTT‑CTTCGCAAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                      <  2:2115840/147‑1 (MQ=255)
                                                                                                                               cAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                      <  1:3756025/150‑1 (MQ=255)
                                                                                                                               cAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                      >  2:226657/1‑150 (MQ=255)
                                                                                                                                   cATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAg                  <  2:3410160/150‑1 (MQ=255)
                                                                                                                                    aTTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGt                 <  1:2389943/150‑1 (MQ=255)
                                                                                                                                     tttc‑CGTCTTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTa                <  1:753780/150‑1 (MQ=255)
                                                                                                                                       tt‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATc              <  2:1026851/150‑1 (MQ=255)
                                                                                                                                            tcgtcATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgc          <  1:100338/150‑1 (MQ=255)
                                                                                                                                            ccgtaATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgc          <  1:3264570/149‑1 (MQ=255)
                                                                                                                                             cgttATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgcg         <  1:3954304/150‑1 (MQ=255)
                                                                                                                                             cgtcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgcg         >  2:2618626/1‑150 (MQ=255)
                                                                                                                                                 aTAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgg     >  1:2078531/1‑150 (MQ=255)
                                                                                                                                                  tAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg    <  2:2322613/150‑1 (MQ=255)
                                                                                                                                                  tAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg    >  2:1066568/1‑150 (MQ=255)
                                                                                                                                                    aaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                        >  2:1136717/1‑96 (MQ=255)
                                                                                                                                                    aaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                        <  1:1136717/96‑1 (MQ=255)
                                                                                                                                                    aaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGc  >  1:2478453/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGC  >  AP012306/1854702‑1854998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑