breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 43.1% | 383.0 / 82.8 | 248 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 1,944,288 | 0 | A | C | 35.4% | 405.9 / 55.4 | 215 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 644,417 | 0 | G | A | 32.5% | 715.5 / inf | 521 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 1,226,557 | 0 | A | G | 30.4% | 355.5 / 91.6 | 181 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 935,054 | 0 | G | T | 29.6% | 186.3 / 42.6 | 81 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 800,675 | 0 | A | C | 29.5% | 616.4 / 35.8 | 278 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 3,938,887 | 0 | C | T | 28.8% | 554.5 / 167.3 | 278 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 2,558,071 | 0 | A | C | 27.9% | 509.4 / 34.1 | 233 | V302G (GTA→GGA) | visC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
* | AP012306 | 202,417 | 0 | T | G | 27.5% | 602.7 / 23.3 | 262 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
* | AP012306 | 3,527,368 | 0 | A | C | 26.2% | 614.1 / 48.8 | 256 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 2,758,909 | 0 | T | G | 25.0% | 635.0 / 18.6 | 241 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 1,854,851 | 0 | A | C | 23.5% | 728.9 / 69.4 | 285 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 3,282,076 | 0 | T | G | 23.2% | 520.0 / 16.7 | 235 | intergenic (‑79/+27) | ilvB/ivbL | acetolactate synthase I, large subunit/ilvB operon leader peptide |
* | AP012306 | 3,184,032 | 0 | A | C | 22.5% | 634.2 / 15.6 | 262 | T159P (ACC→CCC) | yiaO | predicted transporter |
* | AP012306 | 3,456,453 | 0 | A | C | 22.3% | 653.0 / 11.6 | 243 | intergenic (+12/+369) | fre/fadA | flavin reductase/3‑ketoacyl‑CoA thiolase |
* | AP012306 | 3,412,049 | 0 | T | G | 21.8% | 808.4 / 27.4 | 317 | intergenic (+25/‑122) | proM/aslB | tRNA‑Pro/predicted regulator of arylsulfatase activity |
* | AP012306 | 406,677 | 0 | A | G | 21.5% | 489.8 / 18.5 | 187 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 3,324,433 | 0 | T | A | 21.2% | 566.0 / 68.6 | 217 | intergenic (+2/+52) | yieF/yieG | chromate reductase, Class I, flavoprotein/predicted inner membrane protein |
* | AP012306 | 2,499,414 | 0 | A | C | 21.2% | 822.7 / 38.2 | 302 | P126P (CCA→CCC) | yqeI | predicted transcriptional regulator |
* | AP012306 | 524,289 | 0 | T | G | 20.8% | 447.8 / 10.1 | 168 | N4T (AAC→ACC) | citD | citrate lyase, acyl carrier (gamma) subunit |
Marginal new junction evidence (lowest skew 10 of 132 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 1092512 | 393 (1.290) | 20 (0.080) | 12/258 | 10.4 | 6.2% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 268 (1.020) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 997497 | 337 (1.100) | 13 (0.050) | 10/268 | 11.2 | 4.3% | coding (41/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
? | AP012306 | 997993 = | 273 (1.000) | coding (537/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase | |||||
* | ? | AP012306 | = 1092334 | 305 (1.000) | 13 (0.050) | 8/258 | 11.7 | 4.7% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 268 (1.020) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | 1154853 = | 779 (2.550) | 10 (0.030) | 9/292 | 11.9 | 1.3% | coding (416/789 nt) | fabI | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
? | AP012306 | 1154865 = | 770 (2.580) | coding (404/789 nt) | fabI | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent | |||||
* | ? | AP012306 | = 3014868 | 370 (1.210) | 9 (0.030) | 8/274 | 12.0 | 2.6% | coding (930/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
? | AP012306 | = 3014856 | 337 (1.200) | coding (918/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding | |||||
* | ? | AP012306 | = 2105705 | 321 (1.050) | 8 (0.030) | 8/286 | 12.2 | 2.5% | coding (64/900 nt) | hemF | coproporphyrinogen III oxidase |
? | AP012306 | = 2105706 | 307 (1.050) | coding (65/900 nt) | hemF | coproporphyrinogen III oxidase | |||||
* | ? | AP012306 | 1155231 = | 1126 (3.690) | 8 (0.030) | 8/288 | 12.2 | 0.72% | coding (38/789 nt) | fabI | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent |
? | AP012306 | 1155256 = | 1119 (3.800) | coding (13/789 nt) | fabI | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent | |||||
* | ? | AP012306 | 606478 = | 132 (0.430) | 8 (0.030) | 8/292 | 12.3 | 5.4% | coding (153/657 nt) | ybgJ | predicted enzyme subunit |
? | AP012306 | 606497 = | 151 (0.510) | coding (172/657 nt) | ybgJ | predicted enzyme subunit | |||||
* | ? | AP012306 | = 1288775 | 420 (1.380) | 7 (0.020) | 7/278 | 12.4 | 1.7% | coding (577/1683 nt) | ydeN | conserved protein |
? | AP012306 | = 1288765 | 399 (1.400) | coding (587/1683 nt) | ydeN | conserved protein | |||||
* | ? | AP012306 | = 955136 | 265 (0.870) | 7 (0.020) | 7/292 | 12.7 | 2.6% | intergenic (+1265/‑1406) | mdoG/yceK | glucan biosynthesis protein, periplasmic/predicted lipoprotein |
? | AP012306 | = 955148 | 259 (0.870) | intergenic (+1277/‑1394) | mdoG/yceK | glucan biosynthesis protein, periplasmic/predicted lipoprotein |