breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG43.1% 383.0 / 82.8 248intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC35.4% 405.9 / 55.4 215G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306644,4170GA32.5% 715.5 / inf 521intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,226,5570AG30.4% 355.5 / 91.6 181G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP012306935,0540GT29.6% 186.3 / 42.6 81intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP012306800,6750AC29.5% 616.4 / 35.8 278T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123063,938,8870CT28.8% 554.5 / 167.3 278S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123062,558,0710AC27.9% 509.4 / 34.1 233V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP012306202,4170TG27.5% 602.7 / 23.3 262V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123063,527,3680AC26.2% 614.1 / 48.8 256L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123062,758,9090TG25.0% 635.0 / 18.6 241G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123061,854,8510AC23.5% 728.9 / 69.4 285T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,282,0760TG23.2% 520.0 / 16.7 235intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP0123063,184,0320AC22.5% 634.2 / 15.6 262T159P (ACC→CCC) yiaOpredicted transporter
*AP0123063,456,4530AC22.3% 653.0 / 11.6 243intergenic (+12/+369)fre/fadAflavin reductase/3‑ketoacyl‑CoA thiolase
*AP0123063,412,0490TG21.8% 808.4 / 27.4 317intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP012306406,6770AG21.5% 489.8 / 18.5 187K294E (AAA→GAA) hemHferrochelatase
*AP0123063,324,4330TA21.2% 566.0 / 68.6 217intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP0123062,499,4140AC21.2% 822.7 / 38.2 302P126P (CCA→CCCyqeIpredicted transcriptional regulator
*AP012306524,2890TG20.8% 447.8 / 10.1 168N4T (AAC→ACC) citDcitrate lyase, acyl carrier (gamma) subunit

Marginal new junction evidence (lowest skew 10 of 132 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512393 (1.290)20 (0.080) 12/258 10.4 6.2% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 268 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497337 (1.100)13 (0.050) 10/268 11.2 4.3% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 273 (1.000)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092334305 (1.000)13 (0.050) 8/258 11.7 4.7% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 268 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 1154853 =779 (2.550)10 (0.030) 9/292 11.9 1.3% coding (416/789 nt) fabI enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
?AP012306 1154865 = 770 (2.580)coding (404/789 nt) fabI enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
* ? AP012306 = 3014868370 (1.210)9 (0.030) 8/274 12.0 2.6% coding (930/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
?AP012306 = 3014856 337 (1.200)coding (918/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
* ? AP012306 = 2105705321 (1.050)8 (0.030) 8/286 12.2 2.5% coding (64/900 nt) hemF coproporphyrinogen III oxidase
?AP012306 = 2105706 307 (1.050)coding (65/900 nt) hemF coproporphyrinogen III oxidase
* ? AP012306 1155231 =1126 (3.690)8 (0.030) 8/288 12.2 0.72% coding (38/789 nt) fabI enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
?AP012306 1155256 = 1119 (3.800)coding (13/789 nt) fabI enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
* ? AP012306 606478 =132 (0.430)8 (0.030) 8/292 12.3 5.4% coding (153/657 nt) ybgJ predicted enzyme subunit
?AP012306 606497 = 151 (0.510)coding (172/657 nt) ybgJ predicted enzyme subunit
* ? AP012306 = 1288775420 (1.380)7 (0.020) 7/278 12.4 1.7% coding (577/1683 nt) ydeN conserved protein
?AP012306 = 1288765 399 (1.400)coding (587/1683 nt) ydeN conserved protein
* ? AP012306 = 955136265 (0.870)7 (0.020) 7/292 12.7 2.6% intergenic (+1265/‑1406) mdoG/yceK glucan biosynthesis protein, periplasmic/predicted lipoprotein
?AP012306 = 955148 259 (0.870)intergenic (+1277/‑1394) mdoG/yceK glucan biosynthesis protein, periplasmic/predicted lipoprotein