| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP012306 | 472,868 | 0 | T | G | 23.7% | 568.2 / 16.2 | 220 | D435A (GAC→GCC) | cusS | sensory histidine kinase in two‑component regulatory system with CusR, senses copper ions |
| Reads supporting (aligned to +/- strand): ref base T (38/129); new base G (52/0); total (91/129) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-26 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 320 total aligned reads displayed.
TGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTC > AP012306/472719‑473017 | cTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCACCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCa < 1:3211710/150‑1 (MQ=255) aaGGATTAAGCGGGTAATGTGATAACAAACCTTGACCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAAcg < 1:1201225/150‑1 (MQ=255) aGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAAcgc < 2:2360028/150‑1 (MQ=255) aGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAAcgc > 1:2429261/1‑150 (MQ=255) ttAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGaca < 1:234431/150‑3 (MQ=255) ttAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATa < 1:4241435/150‑1 (MQ=255) tAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAg > 1:682323/1‑150 (MQ=255) aGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGaa > 1:3613884/1‑150 (MQ=255) gCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGTCAACGCGATAGaaa < 1:1861878/150‑1 (MQ=255) gCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGaaa < 2:1631857/150‑1 (MQ=255) cGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAAc < 1:3805392/150‑1 (MQ=255) gggTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACg < 1:481790/150‑1 (MQ=255) gTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGt < 1:2169905/150‑1 (MQ=255) gTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGt > 1:3745370/1‑150 (MQ=255) tAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGTc > 2:3616274/1‑150 (MQ=255) tAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGGCACGCGATAGCAAAGGtg > 2:2606899/1‑149 (MQ=255) tgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACa < 2:422567/150‑1 (MQ=255) gATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACaa < 2:58452/150‑1 (MQ=255) cAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATcgcg > 1:2594052/1‑150 (MQ=255) cAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATcccg > 2:3573802/1‑150 (MQ=255) aaaCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCgg > 2:666256/1‑150 (MQ=255) aaaCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATTGCgg > 1:91705/1‑150 (MQ=255) aaaCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCgg > 2:3737932/1‑150 (MQ=255) aaCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGt > 1:4314762/1‑150 (MQ=255) aCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTa < 1:4126545/150‑1 (MQ=255) aCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTa < 1:3474514/150‑1 (MQ=255) ccTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTaa < 1:3277509/150‑1 (MQ=255) cTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAAGGGTCAACGCGATAGAAACGGGCAAACAATCGCGGTAAg > 1:3062579/1‑150 (MQ=255) ttGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGt < 1:2767963/150‑1 (MQ=255) ttGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGt < 2:3144646/150‑1 (MQ=255) tCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTTCACGCGGTAGAAAAGGTCAAACAATCCCGGGAAGTGCt > 1:1010170/1‑150 (MQ=255) cccccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTc < 2:2663054/150‑1 (MQ=255) cccccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTc < 1:2036759/150‑1 (MQ=255) ccGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTCCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGCGGGGGCAACGCGCTAGTAACCGTCAAAAAATCTCGGGCAGTGCTCtgg > 1:1910493/1‑150 (MQ=255) cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGgc < 1:1116301/150‑1 (MQ=255) cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGgc < 1:1718695/150‑1 (MQ=255) gcgcATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATTCCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGgcg < 2:433047/150‑1 (MQ=255) gcATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGGCAAACAATCGCGGGAAGTGCTCGGGCGCa > 2:2740172/1‑150 (MQ=255) aTCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAAt < 2:9484/150‑1 (MQ=255) tCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATg < 2:4385260/150‑1 (MQ=255) ccGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGGCAAACAATCGCGGTAAGGGCTCGGGCGCAATgg > 1:281513/1‑150 (MQ=255) gACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGc < 1:2710919/150‑1 (MQ=255) ttACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTAc > 1:1444114/1‑150 (MQ=255) cAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGt < 1:1539730/150‑1 (MQ=255) cAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAAAAACGGTCAAAAAATCGCGGGAAGTGCTCGGGCGCAATGGGCGTACCcgggg > 2:449824/1‑149 (MQ=255) ccGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGtttt < 1:3436945/150‑1 (MQ=255) ccGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGGGCTCGGGCGCAATGGGGGTACCGGGGgttt > 2:4138453/1‑150 (MQ=255) ccGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACCCGATAGAAAAGGGCAAAAAATCTTGGTAAGTGCGCGGGGGCAAAGGGCGTACCcgggggtt > 2:2852133/1‑146 (MQ=255) cGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTc > 1:293000/1‑150 (MQ=255) tGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCCATGGGCGTACCGGGGGTTTcga > 1:802562/1‑150 (MQ=255) gcgcGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACtt < 1:4064184/150‑1 (MQ=255) cgACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCa < 2:120394/150‑1 (MQ=255) gACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCCATGGGCGTACCGGGGTTTTCGACGATAACTTTCAc > 2:1121684/1‑150 (MQ=255) caacaaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCa > 1:1224253/1‑150 (MQ=255) aacaaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAAATTGCACCAg > 2:3911238/1‑150 (MQ=255) caaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAACGCGATAGAAACGGTCCAACAATCGCGGTAAGGGGTCGGGCGCAAAGGGCGTAACGGGGGTTTTGTCGAAAAATTGCACCAGGt > 2:700859/1‑150 (MQ=255) caaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAggg > 1:1241050/1‑149 (MQ=255) aTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGa > 1:3408320/1‑150 (MQ=255) tCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGAt < 1:3175804/150‑1 (MQ=255) cGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATc < 1:2488588/150‑1 (MQ=255) aTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGTGGGAAGGGTACGGGCGCAATGGGCGTACCGGGGATTTCGACGGTAACTTGCACCAGGTGATCGa > 2:1825231/1‑150 (MQ=255) aTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGGGTACCGGGGGTTTTGACGATAACTTTCACCAGGTGATCGa > 2:531993/1‑150 (MQ=255) aTTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGGACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGa > 2:2971383/1‑150 (MQ=255) tCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCg < 2:913681/150‑1 (MQ=255) cACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGt < 1:1599174/150‑1 (MQ=255) tATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTg < 2:1721370/150‑1 (MQ=255) cGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACa < 1:2236733/150‑1 (MQ=255) gCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAg < 2:3232814/150‑1 (MQ=255) cAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGGACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGGCTGCCAgcg > 2:3382037/1‑150 (MQ=255) cAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAgcg > 2:1540442/1‑150 (MQ=255) gCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGGGGGGGGGAAGCGATAGAAAAGGGGAAACAAAAGCGGGGAGGGGTCGGGGGCAATGGGGGGAACGGGGGTTTCGGCGATAACTTGGACCAGGGGGTTGACCGGCTGAAAggggac > 2:2627914/1‑145 (MQ=255) ccAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACt < 1:2395784/150‑1 (MQ=255) ccAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGGCAAACAATCGCGGTAAGTGCTCGGGGGCGATGGGGGGACCGGGGGTTTTGACGATAACTTGCACCAGGTGATCGACCGGCTGACAGCGCACt > 2:1407157/1‑150 (MQ=255) cAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCCACGCGAAACAAAAGATCCAACAATCGCGGTAAGAGGTCCGGCGCACTCGGCGTACCGGGGGTTTCGACGACAACTTGCACCAGGTGAGCGACCGCCTGACAGCCCGCTa > 2:1988697/1‑150 (MQ=255) cAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCACTa > 2:2880644/1‑150 (MQ=255) cAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAAAGGGCAAACAATCGCGGGAAGGGCTCCGGCGCAAAGGGCGTACCCGGGGTTTTGACGATAACTTGCACCAGGGGATCGACCGTCTGACAacgcccca > 2:2051063/1‑146 (MQ=255) cGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTg < 2:2540212/150‑1 (MQ=255) gCTACCTTCACCTTTTCGCTGGCGGGGGGGGGCAAACGGAAAGGAACGGGCAAACAATCGCGGTAAGCGGTCCGGCGCCATGGGCGGACCGGGGGTTTTGACGAAAAACTCGACCAAGGGATTGACCGTCTGACAGGGCACTTCCATTGt > 2:2220443/1‑150 (MQ=255) gCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGt > 1:736813/1‑150 (MQ=255) gCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGGGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCCCTACAAtttt > 2:3464424/1‑148 (MQ=255) gCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACCGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCCCTACAAtttt > 2:246457/1‑148 (MQ=255) cTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtc > 1:762815/1‑150 (MQ=255) tACCTTCACCTTTTCGCTGGCGGGGGGGGTCAACGCGGTAGAAAAGGTCAAACAATCGCGGGAAGGGCTCGGGGGCAATGGGCGTACCGGGGGTTTTGCCGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCCCCACAATTTtct > 2:3795054/1‑150 (MQ=255) aCCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtctc > 1:4167152/1‑150 (MQ=255) tCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCg < 2:4198531/150‑1 (MQ=255) tCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCg < 2:4259653/150‑1 (MQ=255) tCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCCGACAGCGCACTACAAATGTCTCTCCg > 2:1746376/1‑150 (MQ=255) aCCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGGAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGt > 1:880086/1‑150 (MQ=255) cTTTTCGCTGGCGGGAGGGGTCAACGCGGTAGAAACGGTCAAAAAATCGCGGTAAGGGCTCGGGGGCAATGGGCGGACCGGGGGTTTCGACGATAACTTGCACCAGGGGGTCGACCGTCTGACAGCGCACTACAATTGTTTCTCCGGTTg > 2:1688800/1‑150 (MQ=255) tttCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGGTTTCGACGATAACTTGCACCAGGGGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCCGTTGGc > 2:21550/1‑150 (MQ=255) cTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGtata > 2:3865310/1‑150 (MQ=255) gCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACg < 1:140389/150‑1 (MQ=255) cGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGATGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGc < 1:3993301/150‑1 (MQ=255) cGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGc > 2:1050169/1‑150 (MQ=255) cGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGAAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGc > 2:784463/1‑150 (MQ=255) gAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAgg < 2:1322186/150‑1 (MQ=255) aGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAggg < 2:1512328/150‑1 (MQ=255) aGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAggg > 2:1953652/1‑150 (MQ=255) gggtTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGc < 1:1304082/150‑1 (MQ=255) tCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTc < 2:3319688/150‑1 (MQ=255) | TGCTGAAGGATTAAGCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTGGCGTATAACGCAGGGCGTTC > AP012306/472719‑473017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |