Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306524,2890TG24.2% 328.3 / 13.6 133N4T (AAC→ACC) citDcitrate lyase, acyl carrier (gamma) subunit
Reads supporting (aligned to +/- strand):  ref base T (19/81);  new base G (32/0);  total (52/81)
Fisher's exact test for biased strand distribution p-value = 1.06e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

CGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGC  >  AP012306/524140‑524437
                                                                                                                                                      |                                                                                                                                                     
cGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGt                                                                                                                                                       <  1:2656807/150‑1 (MQ=255)
 gagaACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGtt                                                                                                                                                      <  2:2415264/150‑1 (MQ=255)
 gagaACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGtt                                                                                                                                                      <  2:2090066/150‑1 (MQ=255)
  agaACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGgtt                                                                                                                                                     >  2:1597209/1‑150 (MQ=255)
     aCGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTAtt                                                                                                                                                  >  2:831669/1‑150 (MQ=255)
       gTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGG‑TGGGTtttttt                                                                                                                                                >  2:2289558/1‑150 (MQ=255)
       gTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGG‑CGGtttttttt                                                                                                                                                >  2:3389440/1‑150 (MQ=255)
       gTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTAtttt                                                                                                                                                <  1:3135646/150‑1 (MQ=255)
         ccAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTAttttta                                                                                                                                              >  1:1037109/1‑148 (MQ=255)
          cAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGttttttttttt                                                                                                                                             >  2:2166068/1‑147 (MQ=255)
          cAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCAt                                                                                                                                             <  1:435636/150‑1 (MQ=255)
          cAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑CGGGTTTTTTTTAt                                                                                                                                             >  2:2921674/1‑150 (MQ=255)
            gAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTttttttttttt                                                                                                                                           >  2:1060310/1‑145 (MQ=255)
             aaTGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCAtttt                                                                                                                                          <  1:1449213/150‑1 (MQ=255)
             aaTGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTAGTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCAtttt                                                                                                                                          <  2:1214050/150‑1 (MQ=255)
              aTGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCAttttt                                                                                                                                         <  2:255137/150‑1 (MQ=255)
               tggtggTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTc                                                                                                                                        <  2:1328309/150‑1 (MQ=255)
                ggtggtTCGAATTGCTTCGCCAAACTGTTTCTCAACGCTGCTATTGAGTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTGAAGGGTGCCTGGAACGGGGGGC‑TGGTTTATTTTCATTTTTCa                                                                                                                                       <  2:1740128/150‑1 (MQ=255)
                ggtggtGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCa                                                                                                                                       <  2:938306/150‑1 (MQ=255)
                 gtggtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCAc                                                                                                                                      <  2:763514/150‑1 (MQ=255)
                  tggtgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGG‑TTATTTTTATTTTTCACCt                                                                                                                                    >  1:504116/1‑150 (MQ=255)
                     tgCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGt                                                                                                                                  <  2:4348352/150‑1 (MQ=255)
                       cGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGttt                                                                                                                                <  2:40106/150‑1 (MQ=255)
                        gAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTGTATTTTCATTTTTCACCCGTTtc                                                                                                                               >  1:586022/1‑150 (MQ=255)
                        gAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTTTTTTTATTTTTTTCCCGtttt                                                                                                                               >  2:3186571/1‑149 (MQ=255)
                            tGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCAt                                                                                                                           <  1:1232457/150‑1 (MQ=255)
                              cATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTTTTTTTCTTTTTTTCCCGTTTTTCCTGc                                                                                                                         >  2:3876299/1‑150 (MQ=255)
                              cATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGATTATTTTTCTTTTTGACCTTTTTCTCATgt                                                                                                                         >  1:3446633/1‑149 (MQ=255)
                                tCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCgg                                                                                                                       <  1:2250269/150‑1 (MQ=255)
                                 cGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTCATTTTTCACCTGTTTCTCATGCggg                                                                                                                      >  1:1846417/1‑150 (MQ=255)
                                  gCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTTTTTTCTTTTTTTTCCACTTTCTCCTGCgggg                                                                                                                     >  2:3068600/1‑150 (MQ=255)
                                   ccAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGG‑TGGTTTTTTTTTTTTTTTTACCTGTTTTTCATGCggggg                                                                                                                    >  2:3752377/1‑150 (MQ=255)
                                   ccAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTTTCCTTCggggg                                                                                                                    >  1:3350655/1‑150 (MQ=255)
                                    cAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTTTCATGCgggggg                                                                                                                   >  1:328960/1‑149 (MQ=255)
                                     aaaCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTTCCTGTTTTTCATGCggggggc                                                                                                                  >  2:2769211/1‑148 (MQ=255)
                                       aCTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCtt                                                                                                                <  1:4383706/150‑1 (MQ=255)
                                         tGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTTTCCTGGTTCTCATGCGGGGGTCtttt                                                                                                              >  1:3571495/1‑150 (MQ=255)
                                         tGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCtttt                                                                                                              <  2:2266347/150‑1 (MQ=255)
                                          gTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGG‑TGGGTTTTTTTTTTTTTTTTCCTTTTTTTTCTTTGGGGGGCttttt                                                                                                             >  2:2060133/1‑149 (MQ=255)
                                          gTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTg                                                                                                             <  2:3362969/150‑1 (MQ=255)
                                          gTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTT‑ACCTGTTTCTCATGCGGGGGGCttttta                                                                                                            >  1:1922750/1‑148 (MQ=255)
                                           tttCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCCGTTTCTCATTCGGGGGGCttttta                                                                                                            >  1:3487114/1‑148 (MQ=255)
                                            ttCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGAc                                                                                                           <  2:3913284/150‑1 (MQ=255)
                                            ttCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑GGGATTATTTTCCTTTTTTACCCGTTTCTCATATGGGGGGGTTTTTAc                                                                                                           >  2:2623239/1‑150 (MQ=255)
                                             tctcAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TTGTTTTTTTTTATTTTTCACCTTTTTTTCATGCGGGGGTCTTTTTACg                                                                                                          >  1:3032401/1‑150 (MQ=255)
                                              ctcAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑CGGTTTATTTTCATTTTTTACCTGTTTCTTATGCGGGGGGCTTTTGAcca                                                                                                         >  1:2457864/1‑148 (MQ=255)
                                                cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCGGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTCTCCTTCGGGGGGCTTTTTACGAGc                                                                                                       >  1:4009623/1‑150 (MQ=255)
                                                cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTTTTTTTATTTTTTTCCCGTTTTTCATTCCGGGGGGttttttctagg                                                                                                       >  2:547191/1‑143 (MQ=255)
                                                 aaCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGtt                                                                                                                                                      <  1:4197383/102‑1 (MQ=255)
                                                 aaCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGGGTTTTTACGAGcg                                                                                                      >  2:1658221/1‑149 (MQ=255)
                                                  aCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTTTTTTTTTTTTTTCCCCGTTTTTCATTCGGGGGGCTTTTGGCggggtt                                                                                                     >  2:1268558/1‑145 (MQ=255)
                                                     ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGG‑TGGGTTTTTTTTATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTTCGAGCTGCcg                                                                                                  >  2:1570797/1‑150 (MQ=255)
                                                     ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCGGGGTTTTTTTTCTTTTTTTCCCT‑TTTTTCTTTCGGGGGGCTTTTGACGGGcctccg                                                                                                  >  2:109248/1‑145 (MQ=255)
                                                     ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TCGGTTATTTTTATTTTTCTCCTGTTTCTCATGCGGGGGGCTTTTTACCAGCTGccc                                                                                                  >  1:687888/1‑149 (MQ=255)
                                                      tgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCcgc                                                                                                 <  2:4182276/150‑1 (MQ=255)
                                                        ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTTTCCTGCGGGGGGCTTTTGACGAGCTGCcccgg                                                                                               >  1:1332027/1‑149 (MQ=255)
                                                         tATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTc                                                                                              <  1:45187/150‑1 (MQ=255)
                                                          aTTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTcc                                                                                             <  2:2067786/150‑1 (MQ=255)
                                                           ttGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCt                                                                                            <  2:2243828/150‑1 (MQ=255)
                                                            tGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTTTTTTTATTTTTTACCTGTTTCTCCTGCGGGGGGCTTTTTTCGAGCTGCCCCGGCCTg                                                                                           >  1:4042688/1‑150 (MQ=255)
                                                              aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGc                                                                                         <  1:1175525/150‑1 (MQ=255)
                                                              aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTTTCATTCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGc                                                                                         >  1:587391/1‑150 (MQ=255)
                                                              aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGc                                                                                         >  1:203614/1‑150 (MQ=255)
                                                                ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTCGCgg                                                                                       >  1:861930/1‑150 (MQ=255)
                                                                 tGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTTTTTTTTTTTTTTTCCCGTTTCTTCTTCGGGGGGCTTTTTTCGGGCTGCCGCGTCCTGGCggg                                                                                      >  2:2253287/1‑150 (MQ=255)
                                                                   cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGG‑CGGGTTATTTTTTTTTTTTTCCTGTTTTTTATGCGGGGGGCTTTTTGCGAGCGGCCGCGCTCTGGCggggg                                                                                    >  2:2634393/1‑148 (MQ=255)
                                                                      gTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGc                                                                                 <  2:1977166/150‑1 (MQ=255)
                                                                      gTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCCGTTTTTCATTCGGGGGGCTTTTTTCGAGCTGCCGCGTCCCGGCgggggggg                                                                                 >  2:2356580/1‑145 (MQ=255)
                                                                            atCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc                                                                           <  2:638075/150‑1 (MQ=255)
                                                                                       cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCaa                                                               <  1:3686154/150‑1 (MQ=255)
                                                                                        gAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCaaa                                                              <  1:1052905/150‑1 (MQ=255)
                                                                                          gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAaata                                                            <  2:692341/150‑1 (MQ=255)
                                                                                           tGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAaataa                                                           <  2:4392875/150‑1 (MQ=255)
                                                                                               gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCa                                                       <  1:897093/150‑1 (MQ=255)
                                                                                                 gATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGGTTATTTTTATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTTCGAGCTGCCGCGTCCCGGCGGGGGGGGTCAAGCAGGGTCTTGCaaaaaagccga                                                      >  2:347807/1‑145 (MQ=255)
                                                                                                  aTGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCg                                                    <  1:2617734/150‑1 (MQ=255)
                                                                                                      gTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTTCTTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCCGGCGGGGGGGCGCCAGCAAGGTT‑TTCAAAAAAAACAGCggggc                                                >  2:1850879/1‑146 (MQ=255)
                                                                                                        atcatcACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTg                                              <  2:1406136/150‑1 (MQ=255)
                                                                                                            tcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAgg                                          <  1:2276531/150‑1 (MQ=255)
                                                                                                             cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAggg                                         <  1:3257692/150‑1 (MQ=255)
                                                                                                             cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAggg                                         <  2:1028543/150‑1 (MQ=255)
                                                                                                              acaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGa                                        <  1:1244513/150‑1 (MQ=255)
                                                                                                                   cccAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAgc                                   <  1:2200201/150‑1 (MQ=255)
                                                                                                                          tCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTTTTTTTATTTTTTTCCTGTTTTTCCTGCGGGGGGGTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAGCAGGGTT‑TTCCAAAAAAATAGCGCGGCTGCAGGGGGCCGCGGggcgg                            >  2:2905374/1‑149 (MQ=255)
                                                                                                                            aaGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATa                          <  2:3264060/150‑1 (MQ=255)
                                                                                                                            aaGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATa                          <  2:2496758/150‑1 (MQ=255)
                                                                                                                             aGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAg                         <  1:15981/150‑1 (MQ=255)
                                                                                                                               ggTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGcc                       <  2:305493/150‑1 (MQ=255)
                                                                                                                                  gCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTg                    <  2:2413350/150‑1 (MQ=255)
                                                                                                                                    cTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTgag                  <  1:955965/150‑1 (MQ=255)
                                                                                                                                      gCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGAt                <  1:2058522/150‑1 (MQ=255)
                                                                                                                                          cggcggGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGtt            <  1:1264392/150‑1 (MQ=255)
                                                                                                                                             cggGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGttttt         <  1:2258485/150‑1 (MQ=255)
                                                                                                                                              gggC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCCAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTc        >  2:1874656/1‑150 (MQ=255)
                                                                                                                                              gggC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGGTTTTc        >  1:20056/1‑150 (MQ=255)
                                                                                                                                                gC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGc      >  2:2325942/1‑150 (MQ=255)
                                                                                                                                                    ggTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag   <  2:1349731/150‑1 (MQ=255)
                                                                                                                                                    ggTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGGTTTTCGCcag   >  1:1455802/1‑150 (MQ=255)
                                                                                                                                                     gTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc  >  2:2219873/1‑150 (MQ=255)
                                                                                                                                                     gTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc  >  1:1858070/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGAGAACGTCCAGAATGGTGGTGCGAATTGCATCGCCAAACTGTTTCTCAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGC‑TGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTC‑TGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGC  >  AP012306/524140‑524437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑