Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,128,4120TG21.8% 683.8 / 16.6 258V29G (GTG→GGG) yciOconserved protein
Reads supporting (aligned to +/- strand):  ref base T (37/164);  new base G (56/0);  total (94/164)
Fisher's exact test for biased strand distribution p-value = 6.04e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 317 total aligned reads displayed.

CGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128264‑1128561
                                                                                                                                                    |                                                                                                                                                      
cGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTg                                                                                                                                                       >  1:3728532/1‑150 (MQ=255)
 gCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGa                                                                                                                                                      >  1:3252925/1‑150 (MQ=255)
  cAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGAt                                                                                                                                                     >  1:1618304/1‑150 (MQ=255)
        ttGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTa                                                                                                                                               >  2:2414759/1‑150 (MQ=255)
        ttGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTa                                                                                                                                               >  1:4286970/1‑150 (MQ=255)
          gAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATc                                                                                                                                             <  2:269924/150‑1 (MQ=255)
          gAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATc                                                                                                                                             <  2:3289153/150‑1 (MQ=255)
            aGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCa                                                                                                                                           >  2:1562302/1‑150 (MQ=255)
               cGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑Ct                                                                                                                                       <  1:1370966/150‑1 (MQ=255)
               cGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCCGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑Ct                                                                                                                                       <  2:4087479/150‑1 (MQ=255)
                gTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTg                                                                                                                                      <  2:3170032/150‑1 (MQ=255)
                   tGGCAGCTACGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGAtt                                                                                                                                   <  1:3016651/150‑1 (MQ=255)
                         cTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCt                                                                                                                             >  1:2747867/1‑150 (MQ=255)
                         cTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCt                                                                                                                             <  1:2223217/150‑1 (MQ=255)
                           aTGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTTTTCCA‑CTGCTTCCGGCTaa                                                                                                                           >  2:2013468/1‑149 (MQ=255)
                            tGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTTCCA‑CTGATTTCGGCTATg                                                                                                                          >  1:2069121/1‑150 (MQ=255)
                                       gCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGt                                                                                                               <  2:271669/150‑1 (MQ=255)
                                          gAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTaaa                                                                                                            <  1:469258/150‑1 (MQ=255)
                                          gAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATCCAA‑CTGATTCCGGCTATGCGCGCGGCTGTaaa                                                                                                            >  1:3127585/1‑150 (MQ=255)
                                           aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGGTTCCGGCTATGCGCTCGGCTGTtaaa                                                                                                           >  2:1712033/1‑150 (MQ=255)
                                             caccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAAtt                                                                                                         <  2:917667/150‑1 (MQ=255)
                                              accacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTg                                                                                                        <  1:1334218/150‑1 (MQ=255)
                                                 acaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTTTTTATCCAA‑CTGATTCCGGCTATGCGCTTGGCCGGAAAATTTAAg                                                                                                     >  2:4043480/1‑150 (MQ=255)
                                                  caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCCA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGa                                                                                                    >  1:909996/1‑150 (MQ=255)
                                                  caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGa                                                                                                    <  1:198861/150‑1 (MQ=255)
                                                  caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGa                                                                                                    <  2:1036588/150‑1 (MQ=255)
                                                   agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAt                                                                                                   <  2:4287660/150‑1 (MQ=255)
                                                     agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTTTTTTTCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTTAAGATaa                                                                                                 >  2:187417/1‑150 (MQ=255)
                                                        gggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATaaaaa                                                                                              <  2:1951873/150‑1 (MQ=255)
                                                         ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAc                                                                                             <  2:3687534/150‑1 (MQ=255)
                                                         ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAc                                                                                             <  2:2909946/150‑1 (MQ=255)
                                                          gAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAcg                                                                                            >  2:1776463/1‑150 (MQ=255)
                                                            aTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAcgcg                                                                                          <  2:4201200/150‑1 (MQ=255)
                                                            aTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTTTTTTTCCAA‑CTGATTCCGGTTATGCGCTCGGCTGTAAAATTTAAGATAAAAAcgcg                                                                                          >  2:3770807/1‑150 (MQ=255)
                                                             ttATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGa                                                                                         >  1:1172716/1‑150 (MQ=255)
                                                             ttATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGAGTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGGAAAATTGAAGATAAAAACGCGa                                                                                         >  2:3639232/1‑150 (MQ=255)
                                                              tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGAt                                                                                        <  1:1488294/150‑1 (MQ=255)
                                                              tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGAt                                                                                        <  2:4138550/150‑1 (MQ=255)
                                                              tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCCA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTTAAGATAAAAACGCGAt                                                                                        >  2:3978576/1‑150 (MQ=255)
                                                              tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGAt                                                                                        >  1:2402767/1‑150 (MQ=255)
                                                                tGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATgg                                                                                      <  1:4124977/150‑1 (MQ=255)
                                                                  aGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAg                                                                                    <  2:3497858/150‑1 (MQ=255)
                                                                  aGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTTATTTCGGCTATGGGCTTGGCTGTAAAATTGAAGATAAAAAAGCGATGGAg                                                                                    >  2:1590230/1‑150 (MQ=255)
                                                                   gCCTGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGc                                                                                   <  2:3384762/150‑1 (MQ=255)
                                                                    ccAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCg                                                                                  <  2:3448639/150‑1 (MQ=255)
                                                                    ccAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCg                                                                                  >  1:1454443/1‑150 (MQ=255)
                                                                    ccAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCg                                                                                  <  2:2126074/150‑1 (MQ=255)
                                                                      aGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTa                                                                                >  1:2154958/1‑150 (MQ=255)
                                                                       gTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTAt                                                                               <  1:2840155/150‑1 (MQ=255)
                                                                        tttttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTAtt                                                                              <  2:1450649/150‑1 (MQ=255)
                                                                          tttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTg                                                                            <  2:3812745/150‑1 (MQ=255)
                                                                          tttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTg                                                                            >  1:644197/1‑150 (MQ=255)
                                                                          tttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTg                                                                            >  1:2589116/1‑150 (MQ=255)
                                                                           ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTTTCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAAGCGATGGAGCGTATTTGt                                                                           >  1:2491937/1‑150 (MQ=255)
                                                                           ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGt                                                                           <  1:3105355/150‑1 (MQ=255)
                                                                            tttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTTCAA‑CTGATTTCGGCCATGCGCTCGGCTGTAAAATTTAAGATAAAAAAGCGATGGGGGGGATTTTTc                                                                          >  2:2075296/1‑150 (MQ=255)
                                                                              tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGt                                                                        <  1:2682531/150‑1 (MQ=255)
                                                                               atatTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAACTTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTa                                                                       <  1:3814524/150‑1 (MQ=255)
                                                                                tatTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAAATGAAGATAAAAACGCGATGGAGCGTATTTGTCGTAt                                                                      >  1:1826098/1‑150 (MQ=255)
                                                                                  ttCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTc                                                                    <  1:1017654/150‑1 (MQ=255)
                                                                                       ccTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAg                                                               <  1:2922998/150‑1 (MQ=255)
                                                                                       ccTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAg                                                               <  1:983936/150‑1 (MQ=255)
                                                                                       ccTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCCGCTATGCGCCCGGCTGGAAAATTGAAGATAAAAACGCGATGGAGCGGATTTGTCGTATTTGcccg                                                               >  2:2807094/1‑148 (MQ=255)
                                                                                           aTAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGc                                                           >  1:774671/1‑150 (MQ=255)
                                                                                            tAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTTAAGATAAAAACGCGATGGAGCGGATTTTTCGTATTTGCCAGCGGcc                                                          >  2:2165886/1‑150 (MQ=255)
                                                                                             aaCCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCCGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGGATTCGCCAGCTGCCg                                                         >  1:2246388/1‑150 (MQ=255)
                                                                                                 cacaGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATCCAATCTTA‑TCCCGCTATGCGCTCCGCTGTTAAAATGAAAATAAAAACGAGATTGGGCGTATTTTTCGGATTTTCCCGCTGCCGGTCg                                                     >  2:3108014/1‑150 (MQ=255)
                                                                                                  acaGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCCGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAAGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGAccg                                                    >  1:3924967/1‑148 (MQ=255)
                                                                                                      cAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTcac                                                <  2:1246057/150‑1 (MQ=255)
                                                                                                       aaCGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATTCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTcaca                                               <  2:1746171/150‑1 (MQ=255)
                                                                                                       aaCGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCCA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTcaca                                               >  1:186950/1‑150 (MQ=255)
                                                                                                         cGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTTCGGCTATTCGCTCGGGTGTAAAATTTAAGATAAAAACCCGGTGGAGGGGATTTGTTGTATTCGCCCGCTGCCGGACGGTCCCaaa                                             >  2:2947007/1‑149 (MQ=255)
                                                                                                           tCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACtt                                           <  1:1692876/150‑1 (MQ=255)
                                                                                                           tCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGGTGGAGCGGATTTTTCGTATTCGCCCGCTGCCGGACGGTCCCAAAtt                                           >  2:796432/1‑150 (MQ=255)
                                                                                                            cTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACttt                                          <  2:759923/150‑1 (MQ=255)
                                                                                                            cTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACttt                                          <  1:2314360/150‑1 (MQ=255)
                                                                                                            cTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATCCAA‑CCGATTCCGGCTATGCGCCCGGCTGGAAAATTGAAGATAAAAACGCCGTGGAGCGTAATTGTCGTATTCGCCAGGCGCCGGACGGATACAAAttt                                          >  2:2764694/1‑150 (MQ=255)
                                                                                                               aTCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAcc                                       <  1:3395795/150‑1 (MQ=255)
                                                                                                                tCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAccc                                      <  1:4372597/150‑1 (MQ=255)
                                                                                                                 cAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAAAGCGATGGAGCGGATTTTTCGTATTCGCCAGCTGCCGGGCGGGCACAACTTTACCCt                                     >  2:3118285/1‑150 (MQ=255)
                                                                                                                   aCCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCCGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTTTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCtta                                   >  1:1747427/1‑148 (MQ=255)
                                                                                                                         cGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTc                             <  2:3924203/150‑1 (MQ=255)
                                                                                                                         cGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTc                             <  1:825944/150‑1 (MQ=255)
                                                                                                                          ggTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTcg                            >  1:3537963/1‑150 (MQ=255)
                                                                                                                           gTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTcgc                           <  1:2996990/150‑1 (MQ=255)
                                                                                                                              gagaTCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGAt                        >  2:2975813/1‑150 (MQ=255)
                                                                                                                               agaTCGTGCGTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATc                       >  2:2040257/1‑150 (MQ=255)
                                                                                                                                 aTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGGTTCCGGCTATTCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCtt                     >  2:3567258/1‑150 (MQ=255)
                                                                                                                                  tCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCttt                    <  2:374806/150‑1 (MQ=255)
                                                                                                                                  tCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCttt                    <  1:558911/150‑1 (MQ=255)
                                                                                                                                      gCGTAAAGGCGGGGCGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTTAAGATAAAAAAGCGATGGAGCGCATTTTTCGCATTTGCCCGCTGCCGGAGGGTGACAAATTTAACCTGATGTGTCGCGGTCTTTCTgt                >  2:3224131/1‑149 (MQ=255)
                                                                                                                                        gTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAc              <  1:714126/150‑1 (MQ=255)
                                                                                                                                        gTAAAGGCGGGGGGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAc              >  2:1316689/1‑150 (MQ=255)
                                                                                                                                          aaaGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTTCCCTGATGTGTCGCGATCTTTCTGAActc            <  2:2226178/150‑1 (MQ=255)
                                                                                                                                            aGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  2:2359316/150‑1 (MQ=255)
                                                                                                                                            aGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  1:3612361/150‑1 (MQ=255)
                                                                                                                                              gCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGa        <  1:3450240/150‑1 (MQ=255)
                                                                                                                                                ggggTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAcc      <  2:591293/150‑1 (MQ=255)
                                                                                                                                                ggggTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAcc      >  2:2846848/1‑150 (MQ=255)
                                                                                                                                                ggggTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAcc      <  2:2534215/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAA‑CTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128264‑1128561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑