Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,226,5570AG21.9% 457.9 / 66.4 184G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (21/122);  new base G (40/0);  total (62/122)
Fisher's exact test for biased strand distribution p-value = 3.16e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

TTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226408‑1226706
                                                                                                                                                     |                                                                                                                                                      
  gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtt                                                                                                                                                      >  1:900844/1‑150 (MQ=255)
  gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                      <  1:1770362/150‑1 (MQ=255)
   cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt                                                                                                                                                     >  1:2854309/1‑150 (MQ=255)
   cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt                                                                                                                                                     <  2:1748714/150‑1 (MQ=255)
   cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt                                                                                                                                                     <  2:3718127/150‑1 (MQ=255)
     aTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTta                                                                                                                                                   <  1:2261795/150‑1 (MQ=255)
     aTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTta                                                                                                                                                   <  2:2541945/150‑1 (MQ=255)
      tCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttttt                                                                                                                                                  >  1:1039715/1‑148 (MQ=255)
       cACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata                                                                                                                                                 <  2:2351282/150‑1 (MQ=255)
        aCCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttttttc                                                                                                                                                >  1:2111958/1‑146 (MQ=255)
           ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                                                                                                             <  1:179900/150‑1 (MQ=255)
           ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                                                                                                             <  2:1837150/150‑1 (MQ=255)
                gTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACg                                                                                                                                        <  1:949296/150‑1 (MQ=255)
                   cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAtt                                                                                                                                     <  1:4010883/150‑1 (MQ=255)
                     ggTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTc                                                                                                                                   <  1:4135756/150‑1 (MQ=255)
                        cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTCGGATTTTAcc                                                                                                                                >  2:2669161/1‑150 (MQ=255)
                            gTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGt                                                                                                                            <  1:889303/150‑1 (MQ=255)
                            gTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTCTGACGATTTCACCATGt                                                                                                                            <  2:2034433/150‑1 (MQ=255)
                              aaGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTACGATTTCACCATGtgg                                                                                                                          >  1:3280949/1‑148 (MQ=255)
                              aaGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑Cg                                                                                                                         <  2:2897255/150‑1 (MQ=255)
                                  tgtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTaa                                                                                                                     <  1:1822930/150‑1 (MQ=255)
                                    tCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCCTGT‑CGGTAACg                                                                                                                   >  1:3501611/1‑150 (MQ=255)
                                    tCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACg                                                                                                                   <  1:3603518/150‑1 (MQ=255)
                                    tCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACg                                                                                                                   <  1:3312382/150‑1 (MQ=255)
                                       ggTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTACGGTTTTAACCTGT‑CGGTAAAGccc                                                                                                                >  1:3833211/1‑149 (MQ=255)
                                            cttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTATTTTTTTCGATTTTCCCATTTCCGGTAA‑GCCgggaat                                                                                                           >  2:3413584/1‑146 (MQ=255)
                                            cttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACt                                                                                                           <  2:62282/150‑1 (MQ=255)
                                            cttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACt                                                                                                           <  1:631346/150‑1 (MQ=255)
                                               ctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTTCCATGT‑CGGGAACGCCCGGAATGAc                                                                                                        >  2:889635/1‑150 (MQ=255)
                                                tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTATACTTTTTACGATTTCACCATTT‑CCGTAACGCCGGAAGTGACg                                                                                                       >  1:2941648/1‑150 (MQ=255)
                                                tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACg                                                                                                       <  1:584804/150‑1 (MQ=255)
                                                 ggCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGT‑CGGTAACGCCGGGACTGACGc                                                                                                      >  1:3734014/1‑150 (MQ=255)
                                                    aTAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCCTGT‑CGGTAACGCCGGGACTGACGCTgg                                                                                                   >  1:1305238/1‑150 (MQ=255)
                                                    aTAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTgg                                                                                                   <  1:1712306/150‑1 (MQ=255)
                                                      aaTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCATGT‑CGGTAACGCCGGGACTGACGCTGGCg                                                                                                 >  1:1594239/1‑150 (MQ=255)
                                                       aTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGa                                                                                                <  2:1571273/150‑1 (MQ=255)
                                                            gggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATc                                                                                           <  1:4330905/150‑1 (MQ=255)
                                                             ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGT‑CGGGAACGCCGGGACTGACGCCGGGGGATATcc                                                                                          >  2:2801574/1‑150 (MQ=255)
                                                             ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATcc                                                                                          <  1:2509427/150‑1 (MQ=255)
                                                                tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGTCGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                       >  1:194231/1‑150 (MQ=255)
                                                                 ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACTTTTTTCGATTTTACCATTG‑CCGTAAAGCCGGGACCGGCGCTCGGGAATATccccgg                                                                                      >  2:408001/1‑146 (MQ=255)
                                                                 ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACTTTTTACGGTTTTTCCCTGT‑CGGGAAAGCCGGGAATGGAGCTGGCGGAAATCCCTgc                                                                                      >  2:3749846/1‑150 (MQ=255)
                                                                 ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgc                                                                                      <  1:1878799/150‑1 (MQ=255)
                                                                    gctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt                                                                                   <  2:1406669/150‑1 (MQ=255)
                                                                     ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg                                                                                  <  2:4141944/150‑1 (MQ=255)
                                                                     ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg                                                                                  <  2:1427091/150‑1 (MQ=255)
                                                                     ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg                                                                                  <  1:1538628/150‑1 (MQ=255)
                                                                      tgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGt                                                                                 <  2:1270787/150‑1 (MQ=255)
                                                                       gTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTTACGCTGGCGAATATCCGTGCGCTGtt                                                                                <  1:2337142/150‑1 (MQ=255)
                                                                        tttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGttt                                                                               <  2:4199836/150‑1 (MQ=255)
                                                                         ttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTATTTTTTTCGATTTTACCCTGT‑CGGTAAAGCCCGAACTTACGCTGGCGAATATCCGTGGGCGGTTTa                                                                              >  2:3267387/1‑150 (MQ=255)
                                                                         ttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTCCCCTTT‑CGGTAACGCCCGGAATTACGCCTGGGAAAATCCGTGCCCCGtttt                                                                              >  2:181688/1‑149 (MQ=255)
                                                                          tttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGATTTATAATTTTTACGATTTTACCATGT‑CGGTAACCCCGGAACTGACGCTGGCGGATAACCCTGCCCTGtttta                                                                             >  2:1194887/1‑148 (MQ=255)
                                                                          tttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTTACGGTTTTACCATGT‑CGGTAAACCCGGGACTGACGCTGGCGAATATCCCTGCGCTGtttta                                                                             >  2:4144848/1‑148 (MQ=255)
                                                                          tttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa                                                                             <  1:1294994/150‑1 (MQ=255)
                                                                          tttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa                                                                             <  1:24163/150‑1 (MQ=255)
                                                                           ttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTGCGATTTCACCATGT‑CGGTAACGCCCGGACTGACGCTGGCGAATATCCGTGCGCTGTTTTAt                                                                            >  2:2713839/1‑150 (MQ=255)
                                                                           ttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGATGTTTAAt                                                                            <  2:3126833/150‑1 (MQ=255)
                                                                             aCTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATcc                                                                          <  1:3809948/150‑1 (MQ=255)
                                                                             aCTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATcc                                                                          <  2:919430/150‑1 (MQ=255)
                                                                               tgctgcTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCgg                                                                        <  1:2178162/150‑1 (MQ=255)
                                                                                gctgctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTTCGATTTTACCATGT‑CGGGAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCCGTTTTATCCGGc                                                                       >  2:466611/1‑150 (MQ=255)
                                                                                    ctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAt                                                                   <  2:1857632/150‑1 (MQ=255)
                                                                                     ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAtt                                                                  <  2:942383/150‑1 (MQ=255)
                                                                                      tGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTa                                                                 <  1:3473883/150‑1 (MQ=255)
                                                                                        gCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACg                                                               <  2:4379478/150‑1 (MQ=255)
                                                                                          ccGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAc                                                             <  1:2142396/150‑1 (MQ=255)
                                                                                            gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACCTTTGGCGATTTTCCCATTG‑CGGGAACCCCGGCACTTGCGCTTGGGTAAATCCGTGCGGTGGTTAATCCGGCGGATTACGAcat                                                           >  2:3328942/1‑150 (MQ=255)
                                                                                            gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcat                                                           <  2:2282847/150‑1 (MQ=255)
                                                                                            gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcat                                                           <  1:2820363/150‑1 (MQ=255)
                                                                                               aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcatcat                                                        <  2:2145458/150‑1 (MQ=255)
                                                                                                  gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtct                                                     <  1:4085334/150‑1 (MQ=255)
                                                                                                  gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtct                                                     <  1:973670/150‑1 (MQ=255)
                                                                                                   tgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtctc                                                    <  1:3054412/150‑1 (MQ=255)
                                                                                                      tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCTTTTTACGGTTTTTCCATTT‑CGGTGACGCCCGAACTGGCGCCGGCGGAAATCCCGGGGCTGTTTTATTCCGCGGATTACGAAATCATTCTcccc                                                 >  2:2006853/1‑148 (MQ=255)
                                                                                                      tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAAATCATTCTCCGc                                                 <  1:2305210/150‑1 (MQ=255)
                                                                                                       ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCCTGT‑CGGTAACCCCGGGAATGACGCTGGGGAATATCCCTGCGCCGTTTTATTCCGCGGATTACGAAATCCTTTTCCCCa                                                >  2:3475537/1‑150 (MQ=255)
                                                                                                       ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTTCGGTTTCACCATTT‑CGGGAACGCCCGGACTGACGCTGGCGAATATCCCTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCGCa                                                >  2:600958/1‑150 (MQ=255)
                                                                                                        tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCAc                                               <  1:333671/150‑1 (MQ=255)
                                                                                                         ggCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACg                                              <  1:2685767/150‑1 (MQ=255)
                                                                                                            aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTg                                           <  2:498334/150‑1 (MQ=255)
                                                                                                            aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTg                                           <  1:5641/150‑1 (MQ=255)
                                                                                                                tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGGTTTTACCATGT‑CGGGAACGCCGGAAATGACGCTGGGGGAAATCCGTGGGCTGTTTTATCCGGCGAATTACGACATCATTCTCCCCCCGCTGGCTa                                       >  2:1220555/1‑150 (MQ=255)
                                                                                                                tCTATTTCGGCTCGCGGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTa                                       <  2:3595422/150‑1 (MQ=255)
                                                                                                                     ttCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCt                                  <  1:320614/150‑1 (MQ=255)
                                                                                                                      tCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCCTGT‑TGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCCCACGCTGACTAtaggtg                                 >  2:1003372/1‑145 (MQ=255)
                                                                                                                               tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTcc                        <  2:1524271/150‑2 (MQ=255)
                                                                                                                                gctgACACTGTTATGGCAGGGATTTTATACTTTTTACGATTTTAACATAT‑CGGTAAAGCCCGAACTGACGCCGGCGAATATGCGTGGGCTGTTTTATCCCGCGAATTACGGCAACCATTTCCACACGCTTACTATGGGCGTGGGAGTCAc                       >  2:826270/1‑150 (MQ=255)
                                                                                                                                     cacTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCCGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGCCTATGGCTGTGGCAGGCACTATCg                  >  2:3457292/1‑150 (MQ=255)
                                                                                                                                             tGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGcc          >  2:670898/1‑150 (MQ=255)
                                                                                                                                             tGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGcc          <  2:1498437/150‑1 (MQ=255)
                                                                                                                                                cAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAtt       >  2:354800/1‑150 (MQ=255)
                                                                                                                                                 aGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTc      >  2:4181232/1‑150 (MQ=255)
                                                                                                                                                 aGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTc      >  2:2994120/1‑150 (MQ=255)
                                                                                                                                                  gggATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCt     <  2:3080971/150‑1 (MQ=255)
                                                                                                                                                   ggATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTg    >  2:2226553/1‑150 (MQ=255)
                                                                                                                                                   ggATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGCGGCAGTGACTATCGCCAGCGCCCTTCTg    >  2:4161812/1‑150 (MQ=255)
                                                                                                                                                    gATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg   >  1:2361189/1‑150 (MQ=255)
                                                                                                                                                    gATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg   >  2:3902208/1‑150 (MQ=255)
                                                                                                                                                    gATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg   <  1:32352/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGT‑CGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226408‑1226706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑