Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,116,3540TG22.3% 532.7 / 10.8 221T307P (ACC→CCC) yhjGpredicted outer membrane biogenesis protein
Reads supporting (aligned to +/- strand):  ref base T (62/109);  new base G (49/0);  total (112/109)
Fisher's exact test for biased strand distribution p-value = 3.99e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

TTTTGGTCGTGGCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCAGAGCGGAAATCAGCCT  >  AP012306/3116205‑3116503
                                                                                                                                                     |                                                                                                                                                       
 tttGGTCGTGGCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTa                                                                                                                                                        <  1:2715700/150‑1 (MQ=255)
     gTCGTGGCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAACCCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCgg                                                                                                                                                    <  2:1271152/150‑1 (MQ=255)
     gTCGTGGCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCgg                                                                                                                                                    <  2:3105133/150‑1 (MQ=255)
     gTCGTGGCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCgg                                                                                                                                                    >  2:612215/1‑150 (MQ=255)
         tGGCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGgcag                                                                                                                                                <  1:2973012/150‑1 (MQ=255)
          ggCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGgcagc                                                                                                                                               <  2:4268750/150‑1 (MQ=255)
            cTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGcagcag                                                                                                                                             <  2:2256234/150‑1 (MQ=255)
            cTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGcagcag                                                                                                                                             <  1:3407563/150‑1 (MQ=255)
                ccGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACg                                                                                                                                         >  1:410811/1‑150 (MQ=255)
                ccGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACg                                                                                                                                         >  2:2145882/1‑150 (MQ=255)
                  ggtggtGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGcc                                                                                                                                       <  1:4195722/150‑1 (MQ=255)
                  ggtggtGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCCACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGcc                                                                                                                                       <  2:3304717/150‑1 (MQ=255)
                    tggtgTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGGACGCCCg                                                                                                                                     >  1:3563022/1‑150 (MQ=255)
                     ggtgTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGt                                                                                                                                    >  1:3236751/1‑150 (MQ=255)
                       tgtAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCa                                                                                                                                  <  2:3804844/150‑1 (MQ=255)
                          aGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGtt                                                                                                                               <  2:2297515/150‑1 (MQ=255)
                          aGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGtt                                                                                                                               >  1:1894983/1‑150 (MQ=255)
                           gACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTc                                                                                                                              >  1:1265703/1‑150 (MQ=255)
                            cccAGAGAACCGTGGCTATCGCTATCCCCAATTCGCCCATTAAAACCGCGCTAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑a                                                                                                                            <  2:1542815/149‑1 (MQ=255)
                                   aaCCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGAt                                                                                                                     >  1:1578882/1‑150 (MQ=255)
                                            tatCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTg                                                                                                            <  1:1519024/150‑1 (MQ=255)
                                             atCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAAAACGCCCGTCAGTTC‑ATAAAGATCACCCACTGa                                                                                                           >  2:1101851/1‑150 (MQ=255)
                                               cGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAAt                                                                                                         >  1:929502/1‑150 (MQ=255)
                                                gCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGGGAATc                                                                                                        >  1:2218687/1‑150 (MQ=255)
                                                 cTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCg                                                                                                       <  2:3862186/150‑1 (MQ=255)
                                                 cTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCg                                                                                                       >  2:3617531/1‑150 (MQ=255)
                                                  tATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCTGGCAGCAGAACGCCCCGCCGTTC‑ATAAAGATCACCCAGTGAATCgg                                                                                                      >  2:965864/1‑149 (MQ=255)
                                                   aTCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGcc                                                                                                     >  1:3219804/1‑150 (MQ=255)
                                                   aTCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGcc                                                                                                     <  2:536015/150‑1 (MQ=255)
                                                    tCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCa                                                                                                    >  2:75951/1‑150 (MQ=255)
                                                        cAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGTGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGaaa                                                                                                >  1:223272/1‑150 (MQ=255)
                                                        cAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCCGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCCGaaa                                                                                                >  1:1149706/1‑150 (MQ=255)
                                                         aaTTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCCGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGaaaa                                                                                               >  2:456187/1‑150 (MQ=255)
                                                          aTTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGGGAATCGCCAGaaaaa                                                                                              >  2:125169/1‑149 (MQ=255)
                                                           ttCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAAtt                                                                                             <  2:222254/150‑1 (MQ=255)
                                                           ttCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAAtt                                                                                             <  2:1158068/150‑1 (MQ=255)
                                                            tCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAAttt                                                                                            <  1:1084931/150‑1 (MQ=255)
                                                             cGCCCATTAATACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTa                                                                                           <  1:3129669/150‑1 (MQ=255)
                                                              gCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTaa                                                                                          >  1:1737628/1‑150 (MQ=255)
                                                               cccATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAg                                                                                         <  2:2085158/150‑1 (MQ=255)
                                                                  aTTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCg                                                                                      >  2:3490708/1‑150 (MQ=255)
                                                                   ttAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGt                                                                                     <  1:4236366/150‑1 (MQ=255)
                                                                    tAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑a                                                                                   <  1:475560/150‑1 (MQ=255)
                                                                     aaaaCCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑aa                                                                                  >  1:3692195/1‑150 (MQ=255)
                                                                      aaaCCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑aaa                                                                                 >  1:836927/1‑150 (MQ=255)
                                                                      aaaCCGCGATAATCAAAGACCGACGATTTTTCAGTGACGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑aaa                                                                                 <  2:3063270/150‑1 (MQ=255)
                                                                       aaCCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAAt                                                                                <  2:1511425/150‑1 (MQ=255)
                                                                        aCCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATc                                                                               >  2:2740872/1‑150 (MQ=255)
                                                                           gcgATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGac                                                                            <  2:1383254/150‑1 (MQ=255)
                                                                             gATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGGCGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGG‑AAATTGacac                                                                          >  2:216516/1‑150 (MQ=255)
                                                                              aTAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACAcc                                                                         <  2:1886028/150‑1 (MQ=255)
                                                                              aTAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACAcc                                                                         >  2:1428595/1‑150 (MQ=255)
                                                                                   cAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGGAGAAACCCCGGCAGGTC‑AAAGAGATCACCCCGTTAACCACCAGAAAAATTAAGCCGT‑AAATCGAGAcccaccg                                                                    >  2:79455/1‑145 (MQ=255)
                                                                                   cAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCa                                                                    >  1:2493795/1‑150 (MQ=255)
                                                                                     aaGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCTGGCGGACTCCCCGCAGGTCTGTAAGGATACCCCAG‑GAATTGCCCCGAAATTTAAGCCGG‑AAATGGAACCCGCCCATc                                                                  >  2:2773337/1‑150 (MQ=255)
                                                                                     aaGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATc                                                                  >  1:1357114/1‑150 (MQ=255)
                                                                                          cgacgaTTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCAt                                                             >  2:1816629/1‑150 (MQ=255)
                                                                                            acgaTTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTg                                                           >  1:2443641/1‑150 (MQ=255)
                                                                                             cgaTTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTgg                                                          <  1:1019649/150‑1 (MQ=255)
                                                                                             cgaTTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTgg                                                          >  1:677813/1‑150 (MQ=255)
                                                                                              gaTTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTggg                                                         >  1:54107/1‑150 (MQ=255)
                                                                                                tttttCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTT‑ATAAAGATCACCCAGTGAATCGCCAGAAAATTTTAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTc                                                       >  2:4149629/1‑150 (MQ=255)
                                                                                                tttttCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTc                                                       >  1:1415452/1‑150 (MQ=255)
                                                                                                 ttttCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCa                                                      <  1:2400325/150‑1 (MQ=255)
                                                                                                  tttCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCAt                                                     <  2:3739633/150‑1 (MQ=255)
                                                                                                   ttCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCAtt                                                    >  2:4178339/1‑150 (MQ=255)
                                                                                                     cAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCa                                                  <  2:3363912/150‑1 (MQ=255)
                                                                                                      aGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGAATAGGGGCGCCCAACACCCGCCAGTTC‑ATAGAGATCACCCACTGAATCGCCCGAAAATTTAAGCCGTAAAATCGA‑ACCCCCCCGTTCCATTGGGTCATTCac                                                 >  2:897817/1‑150 (MQ=255)
                                                                                                       gtgtCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCCTCAGTTC‑CTATAGATCACCCAGTGAAACGCCAGAAAATTTAAACCGT‑AAATCGACACCCCCCATCTTCATTTGGTCATTCacg                                                >  2:2030230/1‑150 (MQ=255)
                                                                                                        tgtCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCCGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGGCGTTCccga                                               >  2:1201440/1‑150 (MQ=255)
                                                                                                        tgtCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGGCATTCacga                                               >  2:725855/1‑150 (MQ=255)
                                                                                                         gtCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCacgac                                              <  2:2966631/150‑1 (MQ=255)
                                                                                                         gtCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCacgac                                              <  1:3265201/150‑1 (MQ=255)
                                                                                                           cGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGc                                            >  1:79770/1‑150 (MQ=255)
                                                                                                            gATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGcc                                           >  2:1742394/1‑150 (MQ=255)
                                                                                                             aTTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGGTTCGGGCAGCAGAACACCCCTCAGTTC‑AAAGAGACCACCCAGGGAATCGCCAGAAAACTTCAACCGC‑AAATCGACACCGCCCATCTTCATTTGGTCATTTACGACGccc                                          >  2:788252/1‑149 (MQ=255)
                                                                                                                  cGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGGCCGTCCGATC‑CTAGAGAGCACCCGGTGAATCTCCCGAAAATTTAAGCCGT‑AAATCGGCAACGGCCATCTTCATTGGGGCATTCATGACGCCATCaaa                                     >  2:1709172/1‑150 (MQ=255)
                                                                                                                   gCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCaaaa                                    <  2:2592530/150‑1 (MQ=255)
                                                                                                                    cTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCCTCAAAAg                                   >  1:3540241/1‑150 (MQ=255)
                                                                                                                     tACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGc                                  <  1:2504683/150‑1 (MQ=255)
                                                                                                                     tACCAGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGc                                  >  1:57901/1‑150 (MQ=255)
                                                                                                                      aCCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAAAGATCACCCAGCGAATCGCCAGAAAATTTTAGCCGT‑AAATCGACACCGCCCATCTTCATTTGGTCATTCACGACGCCATCAAAAGCa                                 >  2:2535038/1‑150 (MQ=255)
                                                                                                                        cAGCCGACCATCCGTTTCAAACTGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAac                               >  1:2814361/1‑150 (MQ=255)
                                                                                                                        cAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAac                               >  1:2937070/1‑150 (MQ=255)
                                                                                                                         aGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAaca                              >  1:3794035/1‑150 (MQ=255)
                                                                                                                         aGCCGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAaca                              >  2:2675041/1‑150 (MQ=255)
                                                                                                                           ccGACCATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCCGCAGCTC‑AAAAAGATCACCCAGAGAATCGCCAGAAAATTTAATCCGT‑AAATCGACACCGCCCCTCTTCATTGGGTCATTCCCGACGCCATCAAAAGCAacaca                            >  2:1867720/1‑149 (MQ=255)
                                                                                                                            cGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACgg                           >  1:2656324/1‑150 (MQ=255)
                                                                                                                             gACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCCTCAAAAGCAACACggg                          >  1:3930004/1‑150 (MQ=255)
                                                                                                                             gACCATCCGTTTCAAACGGCGGGGGATCGGGCCGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGGGATTCACGACGCCATCAAAAGCAACACggg                          >  2:457635/1‑150 (MQ=255)
                                                                                                                               ccATCCGTTTCAAACGGCGGGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAAAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGgtg                        >  1:2016539/1‑150 (MQ=255)
                                                                                                                                   ccGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATAGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTAc                    <  1:1827656/150‑1 (MQ=255)
                                                                                                                                      tttCAAACGGCGGTGTATCGGGCAGCACAACGCCCGTCACTTC‑ATAGAGATCCCCCAGTCAATCGCCAGAAAATTTAAGCCGT‑AAATCGACCCCGCCCATCTTCATTGGGTCATTCACCACGCCATCAAAAGCAACACGGGTGTTACCag                 <  2:3909712/150‑1 (MQ=255)
                                                                                                                                      tttCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCag                 >  2:3192429/1‑150 (MQ=255)
                                                                                                                                                cggGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCAGAGCGGAAATc       >  2:3686840/1‑150 (MQ=255)
                                                                                                                                                cggGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCAGAGCGGAAATc       >  2:3248655/1‑150 (MQ=255)
                                                                                                                                                cggGGGATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCAGAGCGGAAATc       >  2:3072548/1‑150 (MQ=255)
                                                                                                                                                 ggggTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCAGAGCGGAAATCa      >  2:4092750/1‑150 (MQ=255)
                                                                                                                                                   ggTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAGCACGGGTGTTACCAGAGCGGAAATCAGc    <  2:3284763/150‑1 (MQ=255)
                                                                                                                                                     tATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCAGAGCGGAAATCAGCCt  >  1:3868298/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TTTTGGTCGTGGCTTTCCGGTGGTGTAGACCAGAGAACCGTGGATATCGCTATCACCAATTCGCCCATTAAAACCGCGATAATCAAAGACCGACGATTTTTCAGTGTCGATTTTCGCTACCAGCCGACCATCCGTTTCAAACGGCGGGGTATCGGGCAGCAGAACGCCCGTCAGTTC‑ATAGAGATCACCCAGTGAATCGCCAGAAAATTTAAGCCGT‑AAATCGACACCGCCCATCTTCATTGGGTCATTCACGACGCCATCAAAAGCAACACGGGTGTTACCAGAGCGGAAATCAGCCT  >  AP012306/3116205‑3116503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑