Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC32.4% 441.3 / 56.1 222L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (126/24);  new base C (0/72);  total (126/96)
Fisher's exact test for biased strand distribution p-value = 8.15e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 278 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                      |                                                                                                                                                     
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAc                                                                                                                                                      <  1:2977838/150‑1 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAccc                                                                                                                                                    >  1:2172489/1‑150 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAccc                                                                                                                                                    <  2:421808/150‑1 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTc                                                                                                                                                  >  2:3424312/1‑150 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTc                                                                                                                                                  <  1:1042290/150‑1 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGAcc                                                                                                                                              >  1:1328099/1‑150 (MQ=255)
                 cGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTcg                                                                                                                                      >  1:1251581/1‑150 (MQ=255)
                 cGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTcg                                                                                                                                      >  1:3459413/1‑150 (MQ=255)
                     cTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCgag                                                                                                                                  >  1:816696/1‑150 (MQ=255)
                       gAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGAt                                                                                                                                >  2:1070695/1‑150 (MQ=255)
                       gAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGAt                                                                                                                                >  2:3350389/1‑150 (MQ=255)
                          tCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCtt                                                                                                                             >  2:2483451/1‑150 (MQ=255)
                            aGTTTCTGCCACAAGGGCTGGAGGGGCAGTATCCGTCTCACTGGCGCGTTAACCACGGCGTATTGCAGAAGGTGC‑GACAGCGGGTTGCACAGATGGAAGAGAAGGAGGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           <  2:4004624/150‑1 (MQ=255)
                            aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           <  1:1761596/150‑1 (MQ=255)
                            aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           <  1:137913/150‑1 (MQ=255)
                             gtggctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCGGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGtt                                                                                                                          <  1:3075819/147‑1 (MQ=255)
                                cctgcCAAAAGAGCTGGAGGGGAAGTATCCGCCCCAGTGGGGCTTAACCCACAGCGTATTGCAGCAGGTGC‑GACAGCTGTTTGCAAAGATGGAACAGCAGAAAGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTAt                                                                                                                       <  2:3011798/149‑1 (MQ=255)
                                 ctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                                      >  2:2913298/1‑150 (MQ=255)
                                    ccACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                   <  2:2410271/150‑1 (MQ=255)
                                    ccACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                   >  2:1521071/1‑150 (MQ=255)
                                     cacaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                  <  1:2450358/150‑1 (MQ=255)
                                     cacaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                  >  2:1969577/1‑150 (MQ=255)
                                     cacaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                  <  1:2801879/150‑1 (MQ=255)
                                       caAGAGCTGGATGGGCAGTATCCGTCTCCCGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGAAGGAAGGGAAAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                                <  2:3568014/150‑1 (MQ=255)
                                         agagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACt                                                                                                              >  2:1477026/1‑150 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTATTTATGCAATTACTg                                                                                                             >  2:3604361/1‑150 (MQ=255)
                                          gagCTGGATGGGCAGTACCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             <  1:359617/150‑1 (MQ=255)
                                           agCTGGAGGGGTATTGTCGGTCTGACGGGCGGGTGCACAACAGTCTTTTGCAGC‑GGTGCGGACCGTTGGTAGCACCCGGGCAACGGCAGGAAGGGGAAATTTATTTACCCGCGTCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGc                                                                                                            <  2:3549128/150‑1 (MQ=255)
                                             ttGGATGGGCAGTACCGGTCTCACTGGCGGGTTAACCACGGCGTATTGCAGCAGGGGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGGAAAATGTTTTCCCCCGGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          <  2:837615/149‑1 (MQ=255)
                                             cTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          >  2:3945269/1‑150 (MQ=255)
                                                     gCAGTCTCCGTCTCACTGGCGGTTTACCCACAGCTTATTGCAGCAGGTGC‑GACAGCTGGTCGCCCAGATGGAACAGCAGGAAGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                  <  2:2605297/150‑1 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                <  1:496787/150‑1 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             <  1:1177728/150‑1 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             <  1:1146209/150‑1 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  2:2112908/1‑150 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  1:3222906/1‑150 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            >  2:931298/1‑150 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            >  1:4359650/1‑150 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAACCACAGCGCATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            >  2:177669/1‑150 (MQ=255)
                                                             cGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCa                                                                                          >  1:1535956/1‑150 (MQ=255)
                                                              gTCTCGCTGGGGCGTTAACCACAGGGTATTGCAGCAGGTGA‑GACAGCTGGTTGCAAAGGTGGAACAGCAGGAGGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         <  2:3840837/150‑1 (MQ=255)
                                                              gTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         >  1:3362186/1‑150 (MQ=255)
                                                                 tcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGttt                                                                                      >  1:2328483/1‑150 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     <  2:3459413/150‑1 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     >  2:3798109/1‑150 (MQ=255)
                                                                        cgcgTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               >  2:3146158/1‑150 (MQ=255)
                                                                        cgcgTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               <  2:3358622/150‑1 (MQ=255)
                                                                         gcgTTACCCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGAAGGAGGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                              <  2:2936849/150‑1 (MQ=255)
                                                                         gcgTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                              >  2:2751572/1‑150 (MQ=255)
                                                                              acccACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         <  1:26459/148‑1 (MQ=255)
                                                                               cccACAGCGTATTGCAGAAGGTGC‑GACAGCTGGTTGCCCAGATGGAACAGCAGGAAGGGGAAAATTATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        <  2:1651651/149‑1 (MQ=255)
                                                                               aCCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        >  2:609360/1‑150 (MQ=255)
                                                                               aCCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAAGGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        <  1:186367/150‑1 (MQ=255)
                                                                                 cacaGCGTTTTGCAGCAGGGGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      <  2:1218003/150‑1 (MQ=255)
                                                                                 cacaGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      <  1:368601/150‑1 (MQ=255)
                                                                                 cacaGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      >  2:1162912/1‑150 (MQ=255)
                                                                                  acaGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaa                                                                     <  2:1064409/150‑1 (MQ=255)
                                                                                   caGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaaa                                                                    >  1:3510207/1‑150 (MQ=255)
                                                                                    aGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAAc                                                                   >  1:1922100/1‑150 (MQ=255)
                                                                                    aGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAAc                                                                   >  1:1852831/1‑150 (MQ=255)
                                                                                       gTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                >  2:1692785/1‑150 (MQ=255)
                                                                                       gTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                >  1:2248756/1‑150 (MQ=255)
                                                                                            gcagcaGGGGC‑GCCAGTTGGTTGCACAGAGGAAACAGAAGGAGGGGAAAAATTTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATc                                                           <  2:2052064/150‑1 (MQ=255)
                                                                                              agcggGTGC‑GACGGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATTATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         <  2:698389/150‑1 (MQ=255)
                                                                                                 agGTGC‑CGCAGCTGGTTGCAGAGATGAAACAGCAGGAGGGGAAAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtc                                                      <  2:2510188/150‑1 (MQ=255)
                                                                                                   gTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtctc                                                    <  1:2571700/150‑1 (MQ=255)
                                                                                                    tGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCa                                                   >  1:2624253/1‑150 (MQ=255)
                                                                                                      c‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCGCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAAc                                                 <  1:1636188/150‑1 (MQ=255)
                                                                                                        gACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                <  2:1892569/150‑1 (MQ=255)
                                                                                                        gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  2:2627542/1‑150 (MQ=255)
                                                                                                         aCAGCTGGTGGCAGAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                               <  1:773417/150‑1 (MQ=255)
                                                                                                          cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              <  1:201205/150‑1 (MQ=255)
                                                                                                          cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              >  1:568897/1‑150 (MQ=255)
                                                                                                             cTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           >  2:4280226/1‑150 (MQ=255)
                                                                                                             cTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           <  1:3753623/150‑1 (MQ=255)
                                                                                                             cTGGTTGCACAGATGAAACAGCAGGAAGGGGAAAATGTTTTCCCCTCGTCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           <  1:639538/150‑1 (MQ=255)
                                                                                                              tGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTc                                          >  2:4397699/1‑150 (MQ=255)
                                                                                                              tGGTTGCAAAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTc                                          <  2:3269575/150‑1 (MQ=255)
                                                                                                               ggTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                         <  2:668205/150‑1 (MQ=255)
                                                                                                                gTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCTCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                        >  1:4223602/1‑150 (MQ=255)
                                                                                                                    cacaGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcct                                    >  2:1241313/1‑150 (MQ=255)
                                                                                                                     acaGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTTCTTctggcctg                                   <  2:3678383/150‑1 (MQ=255)
                                                                                                                         agggAACAGCAGGAGGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               <  2:2835563/148‑1 (MQ=255)
                                                                                                                         aTGGACCGGGAGGAAGGGAAAAATGTTTTCCCTTCGTCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               <  2:2356280/150‑1 (MQ=255)
                                                                                                                         aTGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               <  1:385652/150‑1 (MQ=255)
                                                                                                                         aTGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               >  2:3221026/1‑150 (MQ=255)
                                                                                                                           ggAACAGAAGGAAGGGAAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTgga                             <  2:1398926/150‑1 (MQ=255)
                                                                                                                             aaCAGAAGGAAGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           <  2:3886182/150‑1 (MQ=255)
                                                                                                                              aCAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagga                          >  2:3047090/1‑150 (MQ=255)
                                                                                                                                  cagGAAGGGGAAAATTTTTTCCCCCCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      <  2:3522804/150‑1 (MQ=255)
                                                                                                                                  cagGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      >  2:1574251/1‑150 (MQ=255)
                                                                                                                                     gAAGGGGAAAATTATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtttt                   <  2:3574673/150‑1 (MQ=255)
                                                                                                                                     gAAGGGAAAAATTTTTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtttt                   <  2:4158476/150‑1 (MQ=255)
                                                                                                                                         ggaaaaaTGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               <  2:2162718/147‑1 (MQ=255)
                                                                                                                                           gaaagggATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  1:3974511/144‑1 (MQ=255)
                                                                                                                                           gAAAATTATTAACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  2:2545310/150‑1 (MQ=255)
                                                                                                                                           aaaaaTGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  2:585140/149‑1 (MQ=255)
                                                                                                                                               aTGATTTTCCGTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTGCTGGCCTGGCTGGAGGACCATTTTGCCGATGAgg         <  1:4106058/150‑1 (MQ=255)
                                                                                                                                                   tttCCCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGaa     <  1:3193559/150‑1 (MQ=255)
                                                                                                                                                      aCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTg  <  1:592268/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGC‑GACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑