BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,539,458	C→T	Q106* (CAG→TAG)	cdh →	CDP‑diacylglycerol phosphotidylhydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,539,458	0	C	T	100.0%	1329.9 / NA	338	Q106* (CAG→TAG)	cdh	CDP‑diacylglycerol phosphotidylhydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (162/176); total (162/176)

Only 100 of 354 total aligned reads displayed.

GAAGTCAAACCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGCAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTG  >  AP012306/3539311‑3539604

 aaGTCAAACCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAgg                                                                                                                                                 >  1:809527/1‑150 (MQ=255)

  aGTCAAACCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGgc                                                                                                                                                <  1:1123737/150‑1 (MQ=255)

   gTCAAACCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGgcg                                                                                                                                               <  2:1924813/150‑1 (MQ=255)

   gTCAAACCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGgcg                                                                                                                                               <  2:2633997/150‑1 (MQ=255)

      aaaCCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGt                                                                                                                                            <  1:863288/150‑1 (MQ=255)

      aaaCCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGt                                                                                                                                            >  1:1482452/1‑150 (MQ=255)

        aCCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGa                                                                                                                                          <  1:2760332/150‑1 (MQ=255)

        aCCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGa                                                                                                                                          <  1:2115499/150‑1 (MQ=255)

        aCCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGa                                                                                                                                          >  1:1855750/1‑150 (MQ=255)

         cccAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGAt                                                                                                                                         >  2:336315/1‑150 (MQ=255)

          ccAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGAtt                                                                                                                                        >  1:1604697/1‑150 (MQ=255)

          ccAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGAtt                                                                                                                                        <  1:2233915/150‑1 (MQ=255)

           cAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGAttt                                                                                                                                       >  2:3448182/1‑150 (MQ=255)

               gCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATg                                                                                                                                   <  1:1807369/150‑1 (MQ=255)

               gCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATg                                                                                                                                   >  2:1812465/1‑150 (MQ=255)

                ccGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGa                                                                                                                                  <  2:3903427/150‑1 (MQ=255)

                    aTACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCaa                                                                                                                              <  1:4177040/150‑1 (MQ=255)

                       cGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCaaaaa                                                                                                                           >  2:3296731/1‑150 (MQ=255)

                         tGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAAt                                                                                                                         <  1:1683075/150‑1 (MQ=255)

                         tGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAAt                                                                                                                         <  2:4119133/150‑1 (MQ=255)

                         tGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAAt                                                                                                                         <  1:3538681/150‑1 (MQ=255)

                           gTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATAc                                                                                                                       <  1:4260786/150‑1 (MQ=255)

                           gTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATAc                                                                                                                       <  1:3567903/150‑1 (MQ=255)

                              ttAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGc                                                                                                                    <  1:4025221/150‑1 (MQ=255)

                              ttAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGc                                                                                                                    <  1:4398425/150‑1 (MQ=255)

                                   aGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGcc                                                                                                               >  2:3976959/1‑150 (MQ=255)

                                        ttAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTc                                                                                                          <  1:2999019/150‑1 (MQ=255)

                                         tAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTcc                                                                                                         <  2:3571137/150‑1 (MQ=255)

                                         tAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTcc                                                                                                         <  2:1470432/150‑1 (MQ=255)

                                         tAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTcc                                                                                                         <  2:1106475/150‑1 (MQ=255)

                                             ggCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGAt                                                                                                     >  1:1883477/1‑150 (MQ=255)

                                               cccACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATcg                                                                                                   >  1:2616359/1‑150 (MQ=255)

                                                ccACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATcgc                                                                                                  <  2:3019094/150‑1 (MQ=255)

                                                ccACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATcgc                                                                                                  >  1:3514412/1‑150 (MQ=255)

                                                 cACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATcgcg                                                                                                 >  1:1385029/1‑150 (MQ=255)

                                                   cTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATcgcgcg                                                                                               >  2:2224857/1‑150 (MQ=255)

                                                      cAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGtt                                                                                            <  1:547636/150‑1 (MQ=255)

                                                         tatCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCt                                                                                         >  1:1969177/1‑150 (MQ=255)

                                                            cTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTg                                                                                      <  2:2743578/150‑1 (MQ=255)

                                                            cTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTg                                                                                      <  2:4066302/150‑1 (MQ=255)

                                                            cTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTg                                                                                      >  2:2360344/1‑150 (MQ=255)

                                                              gTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGc                                                                                    <  1:363244/150‑1 (MQ=255)

                                                              gTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGc                                                                                    <  1:1540891/150‑1 (MQ=255)

                                                                  aTGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATc                                                                                >  1:764822/1‑150 (MQ=255)

                                                                  aTGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATc                                                                                >  1:4328601/1‑150 (MQ=255)

                                                                      cAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACt                                                                            >  1:2023480/1‑150 (MQ=255)

                                                                      cAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGAAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACt                                                                            >  2:4247226/1‑150 (MQ=255)

                                                                        aCGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTcc                                                                          <  1:1178441/150‑1 (MQ=255)

                                                                             tcgtatTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCAc                                                                     <  1:3285503/150‑1 (MQ=255)

                                                                                 atTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCggg                                                                 <  1:2644636/150‑1 (MQ=255)

                                                                                   tAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCg                                                               >  1:987695/1‑150 (MQ=255)

                                                                                     aCGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTa                                                             <  2:599896/150‑1 (MQ=255)

                                                                                     aCGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTa                                                             >  2:3199864/1‑150 (MQ=255)

                                                                                     aCGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTa                                                             >  1:2897964/1‑150 (MQ=255)

                                                                                      cGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTAc                                                            >  1:2161072/1‑150 (MQ=255)

                                                                                       ggTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACg                                                           <  1:652147/150‑1 (MQ=255)

                                                                                        gTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGc                                                          <  2:3810186/150‑1 (MQ=255)

                                                                                         tACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCa                                                         >  2:1519154/1‑150 (MQ=255)

                                                                                              aaaGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCCAAAcc                                                    >  2:3110665/1‑150 (MQ=255)

                                                                                              aaaGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAAcc                                                    >  1:3028516/1‑150 (MQ=255)

                                                                                                aGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCAt                                                  <  1:596737/150‑1 (MQ=255)

                                                                                                aGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCAt                                                  >  1:3281239/1‑150 (MQ=255)

                                                                                                   ccTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCAtttt                                               <  2:2541781/150‑1 (MQ=255)

                                                                                                        gttgACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTttcatat                                          <  2:280540/150‑1 (MQ=255)

                                                                                                         ttgACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTGGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTttcatatt                                         <  2:1347520/150‑1 (MQ=255)

                                                                                                          tgACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTttcatattc                                        <  2:4384252/150‑1 (MQ=255)

                                                                                                            aCCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCTAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTttcatattcat                                      >  1:1426361/1‑150 (MQ=255)

                                                                                                             ccGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTttcatattcata                                     <  2:106516/150‑1 (MQ=255)

                                                                                                               gATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATAtc                                   >  2:3692529/1‑150 (MQ=255)

                                                                                                                aTCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATAtct                                  <  2:2972075/150‑1 (MQ=255)

                                                                                                                  ccTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATctct                                <  1:3944050/150‑1 (MQ=255)

                                                                                                                   cTTCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCtt                               <  2:1969177/150‑1 (MQ=255)

                                                                                                                    ttCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTg                              >  1:2784187/1‑150 (MQ=255)

                                                                                                                    ttCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGA‑TTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGt                             <  2:1701735/150‑1 (MQ=255)

                                                                                                                     tCAACGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGt                             <  1:2848300/150‑1 (MQ=255)

                                                                                                                       aaCGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTAt                           >  2:129089/1‑150 (MQ=255)

                                                                                                                       aaCGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTAt                           <  1:3842468/150‑1 (MQ=255)

                                                                                                                        aCGCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCCTATCTCTTGTAtt                          >  2:894134/1‑150 (MQ=255)

                                                                                                                          gCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCg                        >  1:2477317/1‑150 (MQ=255)

                                                                                                                          gCCGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCg                        <  2:2116731/150‑1 (MQ=255)

                                                                                                                           ccGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGt                       <  2:4331481/150‑1 (MQ=255)

                                                                                                                            cGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTc                      >  1:3428303/1‑150 (MQ=255)

                                                                                                                            cGAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTc                      <  1:2394252/150‑1 (MQ=255)

                                                                                                                             gAACTTCTTTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTcc                     >  1:3101563/1‑150 (MQ=255)

                                                                                                                                 ttcttTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGAt                 >  1:4238894/1‑150 (MQ=255)

                                                                                                                                  tcttTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGAtg                >  1:2686481/1‑150 (MQ=255)

                                                                                                                                  tcttTTGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGAtg                <  2:528729/150‑1 (MQ=255)

                                                                                                                                     tttGGTTGGCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTgc             <  1:1719132/150‑1 (MQ=255)

                                                                                                                                       tggttggCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTgcgc           <  2:1201060/150‑1 (MQ=255)

                                                                                                                                       tggttggCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTgcgc           <  1:1007147/150‑1 (MQ=255)

                                                                                                                                         gttggCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCaa         >  1:1241858/1‑150 (MQ=255)

                                                                                                                                         gttggCTTGGTAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCaa         >  1:4116357/1‑150 (MQ=255)

                                                                                                                                          ttggcttggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCaaa        >  1:1362210/1‑150 (MQ=255)

                                                                                                                                           tggcttggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAAc       >  2:805973/1‑150 (MQ=255)

                                                                                                                                             gcttggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAg     <  1:3226379/150‑1 (MQ=255)

                                                                                                                                             gcttggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAg     <  1:492064/150‑1 (MQ=255)

                                                                                                                                              cttggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGc    >  1:936863/1‑150 (MQ=255)

                                                                                                                                               ttggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCt   >  1:3755564/1‑150 (MQ=255)

                                                                                                                                               ttggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCt   <  1:2297803/150‑1 (MQ=255)

                                                                                                                                                tggtAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTg  >  2:2070656/1‑150 (MQ=255)

GAAGTCAAACCCAATGCCGGATACGTGGTTTTAAAAGATCTTAATGGCCCACTGCAATATCTGTTGATGCCAACGTATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGCAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTG  >  AP012306/3539311‑3539604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`