| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AP012306 | 1,132,860 | T→C | N161S (AAC→AGC) | yciK ← | predicted oxoacyl‑(acyl carrier protein) reductase, EmrKY‑TolC system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP012306 | 1,132,860 | 0 | T | C | 99.0% | 1124.3 / ‑1.2 | 286 | N161S (AAC→AGC) | yciK | predicted oxoacyl‑(acyl carrier protein) reductase, EmrKY‑TolC system |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (151/132); total (153/133) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 291 total aligned reads displayed.
TCTTCGGTCGGGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTT > AP012306/1132713‑1133006 | cTTCGGTCGGGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTgg > 2:428282/1‑150 (MQ=255) cTTCGGTCGGGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTgg > 2:3557586/1‑150 (MQ=255) ttCGGTCGGGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGc < 1:4063388/150‑1 (MQ=255) ggtcggGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCg > 1:856674/1‑150 (MQ=255) gtcggGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGt < 2:3900653/150‑1 (MQ=255) tcggGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTc < 2:2127523/150‑1 (MQ=255) gggAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTccc > 1:2383254/1‑150 (MQ=255) ggAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGTCCCt < 2:4298437/150‑1 (MQ=255) ggAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCt < 2:2579476/150‑1 (MQ=255) ggAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCt > 2:1070228/1‑150 (MQ=255) aaGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGa > 2:3838971/1‑150 (MQ=255) ggCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACg < 1:1090619/150‑1 (MQ=255) gcgcTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGt < 2:2451289/150‑1 (MQ=255) gcgcTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGt < 1:4303797/150‑1 (MQ=255) gcgcTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGt > 2:3956495/1‑150 (MQ=255) gcgcTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGt < 1:3219720/150‑1 (MQ=255) gCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACg > 2:97768/1‑150 (MQ=255) aCGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCt > 2:1317066/1‑150 (MQ=255) aCGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCt > 2:53236/1‑150 (MQ=255) gCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTtg > 2:3511285/1‑150 (MQ=255) aTTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTtgat < 2:1107031/150‑1 (MQ=255) aTTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTtgat > 1:4326368/1‑150 (MQ=255) aTTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTtgat > 1:452789/1‑150 (MQ=255) gCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGaa > 1:278856/1‑150 (MQ=255) cGGTGCGCGTACCGCCTGGGATAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAg > 2:2026991/1‑150 (MQ=255) ggTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGt < 2:2267570/150‑1 (MQ=255) gTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTa > 1:1729349/1‑150 (MQ=255) gTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTa < 1:2680799/150‑1 (MQ=255) gTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTa > 2:1558227/1‑150 (MQ=255) gcgcGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTaaa < 1:123272/150‑1 (MQ=255) gcgcGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTaaa > 1:1989167/1‑150 (MQ=255) gcgcGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTaaa > 1:231151/1‑150 (MQ=255) cgcgTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAg < 2:1579024/150‑1 (MQ=255) gcgTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGGGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGa > 2:1484571/1‑150 (MQ=255) cgTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑c < 1:663350/150‑1 (MQ=255) gTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑cc > 1:3791232/1‑150 (MQ=255) tACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCa > 2:895846/1‑150 (MQ=255) tACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCa > 1:2010254/1‑150 (MQ=255) cGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTg < 1:259154/150‑1 (MQ=255) cGCCTGGGTTAATGCAGTTGACACGCAGGCGCGACTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTAGACGCTGCATATGCCCCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTg < 2:4063967/150‑1 (MQ=255) ccTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGaa > 2:1976703/1‑150 (MQ=255) tGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAAcc > 1:1081947/1‑150 (MQ=255) tGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAAcc < 2:629115/150‑1 (MQ=255) tGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAAcc > 1:1462101/1‑150 (MQ=255) ttAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCg > 1:2868447/1‑150 (MQ=255) tAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGt > 1:4135382/1‑150 (MQ=255) aaTGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTc > 1:2945963/1‑150 (MQ=255) aTGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTcc < 1:1433264/150‑1 (MQ=255) gTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGAttt < 2:4041312/150‑1 (MQ=255) gTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGAttt < 1:1677202/150‑1 (MQ=255) gTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGAttt < 1:1146413/150‑1 (MQ=255) ttGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTg > 2:2899684/1‑150 (MQ=255) tGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGa > 1:3588813/1‑150 (MQ=255) cAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGtaataa < 2:4366072/150‑1 (MQ=255) ggCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAg < 2:4137571/150‑1 (MQ=255) gcgcTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAgg > 1:919202/1‑150 (MQ=255) tgctgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGc < 1:3737238/150‑1 (MQ=255) tgctgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGc < 1:4286196/150‑1 (MQ=255) gctgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCa > 1:2180855/1‑150 (MQ=255) gctgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCa > 1:4357346/1‑150 (MQ=255) gctgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCa > 1:514261/1‑150 (MQ=255) ctgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAg < 1:2437395/150‑1 (MQ=255) tgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGt > 1:1568829/1‑150 (MQ=255) tgATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGt > 1:741858/1‑150 (MQ=255) atTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCt > 2:2238906/1‑150 (MQ=255) aTCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGt < 2:1725995/150‑1 (MQ=255) ggCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAg < 2:2082560/150‑1 (MQ=255) gCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGc > 1:2134251/1‑150 (MQ=255) gCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGc > 1:3842775/1‑150 (MQ=255) ccAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCa > 1:1396189/1‑150 (MQ=255) cAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCAt > 2:3696252/1‑150 (MQ=255) gTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGGCGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATaa < 2:3040822/150‑1 (MQ=255) gTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATaa < 1:602978/150‑1 (MQ=255) aCCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAg > 1:1818907/1‑150 (MQ=255) cTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGt > 1:1325243/1‑150 (MQ=255) atcatcCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCa < 1:3115778/150‑1 (MQ=255) tcatcCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGGGAGCATAAAGGTGGCAt > 1:3887524/1‑150 (MQ=255) catcCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCAtt > 2:838346/1‑150 (MQ=255) ccTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACg > 1:3325120/1‑150 (MQ=255) ttCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGtt < 2:311328/150‑1 (MQ=255) ttCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGtt < 1:249759/150‑1 (MQ=255) tCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTg < 1:1742362/150‑1 (MQ=255) tCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTg > 2:2799318/1‑150 (MQ=255) ggTGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGAc > 1:3443291/1‑150 (MQ=255) tGGCAAATTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCt > 1:945210/1‑150 (MQ=255) aaaTTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCAt < 2:3808036/150‑1 (MQ=255) aaaTTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCAt < 2:1767183/150‑1 (MQ=255) aTTTCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGa > 1:2560705/1‑150 (MQ=255) tttCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGAc < 2:3248367/150‑1 (MQ=255) tCGACGCTGCATATGCACCCCAGCTGGCTCGTCCCGGCCGTCC‑ACCCTTGGAGAAAGAAAGACCCAGGAAACCGCCGT‑CGATTTTATGAAAAAAAGAAGCCGTGCCCTGGGGGGGCAAAAGGGGGGCATTACCTTGGACCTGCAtacggc > 2:1530734/1‑145 (MQ=255) tGCATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCa > 2:797566/1‑150 (MQ=255) cATATGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGa > 2:1374045/1‑150 (MQ=255) atatGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGAc > 1:4213767/1‑150 (MQ=255) atGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCt > 1:3561648/1‑150 (MQ=255) tGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTg < 1:3137146/150‑1 (MQ=255) tGCACCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTg < 2:1819761/150‑1 (MQ=255) aCCCCAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCgg > 2:3581768/1‑150 (MQ=255) cccAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGAt < 1:3965196/150‑1 (MQ=255) cccAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGAt > 1:3037376/1‑150 (MQ=255) cAGCTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGAttt > 2:2391507/1‑150 (MQ=255) | TCTTCGGTCGGGAAGGCGCTGGCACGCATTGCGGTGCGCGTACCGCCTGGGTTAATGCAGTTGACACGCAGGCGCTGCTGATATTCATCGGCCAGTACCTGCATCATCCCTTCGGTGGCAAATTTCGACGCTGCATATGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGA‑CCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTG‑CCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTT > AP012306/1132713‑1133006 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |