Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 2,791,735 +A intergenic (‑113/‑235) metY ← / → argG tRNA‑Met/argininosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,791,7341.A98.7% 1564.8 / 8.3 396intergenic (‑112/‑236)metY/argGtRNA‑Met/argininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base A (190/201);  total (192/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 410 total aligned reads displayed.

TTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCAC‑ATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCGATAAAAGAT  >  AP012306/2791584‑2791878
                                                                                                                                                       |                                                                                                                                                
 tATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATCCTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCcac                                                                                                                                                   <  2:555470/150‑1 (MQ=255)
 tATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCcac                                                                                                                                                   >  2:2201087/1‑150 (MQ=255)
    gAGCCAGACGAGCTACCAGGCTGCTCCCCCCCGCGCCTGAAACGTGGCAAATGCTACTCGTTTGGGGTAAAAAATGAAAATACTGCTGGGATTTGGTGTCCCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCcac‑aat                                                                                                                                               <  2:1675075/150‑3 (MQ=255)
          gacgaGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTc                                                                                                                                          >  1:70453/1‑150 (MQ=255)
          gacgaGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTc                                                                                                                                          <  1:607752/150‑1 (MQ=255)
            cgaGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCaa                                                                                                                                        >  2:882173/1‑150 (MQ=255)
            cgaGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCaa                                                                                                                                        <  2:3209194/150‑1 (MQ=255)
             gaGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAAc                                                                                                                                       <  1:665378/150‑1 (MQ=255)
                cTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGtt                                                                                                                                    <  1:1242366/150‑1 (MQ=255)
                   ccAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTAt                                                                                                                                 <  1:1677449/150‑1 (MQ=255)
                     aGGCTGCTCCCCCCCGCGCCTGAAACGTGGCAAATTCTCCTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTg                                                                                                                               <  2:341729/150‑1 (MQ=255)
                     aGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTg                                                                                                                               >  1:1280953/1‑150 (MQ=255)
                     aGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTg                                                                                                                               <  2:476643/150‑1 (MQ=255)
                       gctgctCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCt                                                                                                                             <  2:2038009/150‑1 (MQ=255)
                        ctgctCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACTTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTa                                                                                                                            <  2:2133937/150‑1 (MQ=255)
                        ctgctCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTa                                                                                                                            >  2:3963571/1‑150 (MQ=255)
                            tCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATCCTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCa                                                                                                                        <  2:2537422/150‑1 (MQ=255)
                            tCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCa                                                                                                                        <  2:1158734/150‑1 (MQ=255)
                              cACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGCAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGGTTATTGCTAATCAtg                                                                                                                      >  2:246676/1‑150 (MQ=255)
                                      gcCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGtgaatgaa                                                                                                              >  1:3433375/1‑150 (MQ=255)
                                      gcCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGtgaatgaa                                                                                                              >  1:4136160/1‑150 (MQ=255)
                                      gcCAGAAACGTGGCAAATTCTACTCGTTTGGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGtgaatgaa                                                                                                              <  2:1204844/150‑1 (MQ=255)
                                       ccTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTgaatgaat                                                                                                             >  1:511450/1‑150 (MQ=255)
                                       ccTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTgaatgaat                                                                                                             <  1:2840212/150‑1 (MQ=255)
                                       ccTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTgaatgaat                                                                                                             <  1:1494257/150‑1 (MQ=255)
                                       ccTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTgaatgaat                                                                                                             >  2:3006612/1‑150 (MQ=255)
                                        cTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAata                                                                                                            >  2:1442597/1‑150 (MQ=255)
                                         tGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAatat                                                                                                           >  2:2965033/1‑150 (MQ=255)
                                         tGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAatat                                                                                                           <  1:3877249/150‑1 (MQ=255)
                                          gAACCGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATCCTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATc                                                                                                          <  2:273268/150‑1 (MQ=255)
                                          gAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATCCTGCTGGAATTTGGTGTCCCGAGACGGGAGGTAAAATCTGCAGCCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATc                                                                                                          <  2:539064/150‑1 (MQ=255)
                                          gAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATc                                                                                                          >  2:1301094/1‑150 (MQ=255)
                                            aaCGTGGCAATTTCTACTTGTTTGGGTAAAAAAATGAAAATACTGCTGGATTTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCa                                                                                                        <  2:3077776/150‑1 (MQ=255)
                                             aCGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAg                                                                                                       >  1:83798/1‑150 (MQ=255)
                                             aCGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAg                                                                                                       <  1:3379379/150‑1 (MQ=255)
                                              cGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGt                                                                                                      >  1:2772570/1‑150 (MQ=255)
                                                tGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTc                                                                                                    >  1:3581401/1‑150 (MQ=255)
                                                tGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTc                                                                                                    <  1:875302/150‑1 (MQ=255)
                                                  gCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCAc                                                                                                  <  1:921876/150‑1 (MQ=255)
                                                   cAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACt                                                                                                 >  1:379713/1‑150 (MQ=255)
                                                      aTTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTc                                                                                              <  2:712840/150‑1 (MQ=255)
                                                       ttCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCa                                                                                             >  1:959689/1‑150 (MQ=255)
                                                            cTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATttgt                                                                                        >  2:1525079/1‑150 (MQ=255)
                                                               gTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGa                                                                                     <  1:3557661/150‑1 (MQ=255)
                                                               gTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGa                                                                                     <  1:1404356/150‑1 (MQ=255)
                                                                 ttggggTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAAt                                                                                   <  2:649335/147‑1 (MQ=255)
                                                                  ttGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATa                                                                                  >  1:991448/1‑150 (MQ=255)
                                                                   tGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATAc                                                                                 >  2:3541592/1‑150 (MQ=255)
                                                                    gggTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACt                                                                                <  1:1790612/150‑1 (MQ=255)
                                                                     ggTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACtt                                                                               <  1:2969281/150‑1 (MQ=255)
                                                                     ggTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACtt                                                                               >  1:1196347/1‑150 (MQ=255)
                                                                       tAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACtttt                                                                             <  2:762902/150‑1 (MQ=255)
                                                                       tAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACtttt                                                                             <  1:2019110/150‑1 (MQ=255)
                                                                        aaaaaaTGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTg                                                                            <  2:1724768/150‑1 (MQ=255)
                                                                         aaaaaTGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGc                                                                           >  2:4030029/1‑150 (MQ=255)
                                                                         aaaaaTGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTCGc                                                                           <  1:2078752/150‑1 (MQ=255)
                                                                           aaaTGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCt                                                                         >  1:3615874/1‑150 (MQ=255)
                                                                             aTGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTtc                                                                       >  1:1977401/1‑150 (MQ=255)
                                                                                 aaaTACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCt                                                                   >  1:2361406/1‑150 (MQ=255)
                                                                                    tACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGct                                                                >  2:1839553/1‑150 (MQ=255)
                                                                                      ctgctgGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGctct                                                              >  1:1938307/1‑150 (MQ=255)
                                                                                        gctgGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTcc                                                            >  2:3671396/1‑150 (MQ=255)
                                                                                         ctgGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTccc                                                           >  1:2254056/1‑150 (MQ=255)
                                                                                          tgGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCt                                                          <  2:290682/150‑1 (MQ=255)
                                                                                              aTTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAg                                                      <  2:348210/150‑1 (MQ=255)
                                                                                                    tgtACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCatt                                                >  2:3697716/1‑150 (MQ=255)
                                                                                                     gtACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCatta                                               <  1:733334/150‑1 (MQ=255)
                                                                                                        ccGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTAttt                                            <  2:3364313/150‑1 (MQ=255)
                                                                                                        ccGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAACCGCATTAttt                                            >  2:1081515/1‑150 (MQ=255)
                                                                                                         cGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTAtttt                                           <  1:758007/150‑1 (MQ=255)
                                                                                                           aggcGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTAc                                         <  2:4081818/147‑1 (MQ=255)
                                                                                                           agaCGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTAc                                         >  1:3138544/1‑150 (MQ=255)
                                                                                                           agaCGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTAc                                         <  1:1017621/150‑1 (MQ=255)
                                                                                                            gaCGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACa                                        <  2:3846697/150‑1 (MQ=255)
                                                                                                            gaCGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACa                                        >  2:2008697/1‑150 (MQ=255)
                                                                                                            gaCGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACa                                        <  2:1950288/150‑1 (MQ=255)
                                                                                                                 gACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACaaaaaa                                   <  1:3719159/150‑1 (MQ=255)
                                                                                                                 gACGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACaaaaaa                                   <  1:2574011/150‑1 (MQ=255)
                                                                                                                  aCGTAAAATCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAac                                  >  2:664707/1‑150 (MQ=255)
                                                                                                                      aaaaTCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAacacac                              >  2:3129899/1‑150 (MQ=255)
                                                                                                                       aaaTCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACt                             >  2:2608245/1‑150 (MQ=255)
                                                                                                                        aaTCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTa                            >  2:61444/1‑150 (MQ=255)
                                                                                                                          tCTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTaaa                          >  1:1111119/1‑150 (MQ=255)
                                                                                                                           cTGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAAc                         >  1:2115038/1‑150 (MQ=255)
                                                                                                                            tGCAGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAAct                        >  1:3324547/1‑150 (MQ=255)
                                                                                                                               aGGCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCtt                     >  1:1630153/1‑150 (MQ=255)
                                                                                                                                ggCATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTc                    >  1:4013926/1‑150 (MQ=255)
                                                                                                                                  cATTATAGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCt                  <  2:2281237/150‑1 (MQ=255)
                                                                                                                                     tataGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGtc               <  1:1875445/150‑1 (MQ=255)
                                                                                                                                      ataGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGtct              <  1:2683305/150‑1 (MQ=255)
                                                                                                                                      ataGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGtct              >  2:3274228/1‑150 (MQ=255)
                                                                                                                                       taGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGtctc             >  1:3939709/1‑150 (MQ=255)
                                                                                                                                       taGTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGtctc             >  2:1545492/1‑150 (MQ=255)
                                                                                                                                         gTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCg           >  2:1129662/1‑150 (MQ=255)
                                                                                                                                         gTGATCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCg           >  1:242418/1‑150 (MQ=255)
                                                                                                                                             tCCACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCGATaa       <  1:319223/150‑1 (MQ=255)
                                                                                                                                              ccACGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCGATaaa      >  2:1884023/1‑150 (MQ=255)
                                                                                                                                                 cGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCGATAAAAga   >  1:3248734/1‑150 (MQ=255)
                                                                                                                                                 cGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCGATAAAAga   >  1:1534253/1‑150 (MQ=255)
                                                                                                                                                 cGCCACAATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCGATAAAAga   <  2:835458/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
TTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGCCTGAAACGTGGCAAATTCTACTCGTTTTGGGTAAAAAATGCAAATACTGCTGGGATTTGGTGTACCGAGACGGGACGTAAAATCTGCAGGCATTATAGTGATCCACGCCAC‑ATTTTGTCAACGTTTATTGCTAATCATGTGAATGAATATCCAGTTCACTTTCATTTGTTGAATACTTTTGCCTTCTCCTGCTCTCCCTTAAGCGCATTATTTTACAAAAAACACACTAAACTCTTCCTGTCTCCGATAAAAGAT  >  AP012306/2791584‑2791878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑