Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG44.2% 373.4 / 76.1 252intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
Reads supporting (aligned to +/- strand):  ref base T (40/100);  new base G (111/0);  total (152/100)
Fisher's exact test for biased strand distribution p-value = 1.79e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 271 total aligned reads displayed.

CTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGT  >  AP012306/1091844‑1092141
                                                                                                                                                    |                                                                                                                                                     
cTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGtt                                                                                                                                                      >  2:1409435/1‑150 (MQ=255)
    ggATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGgttttt                                                                                                                                                  >  2:2399650/1‑150 (MQ=255)
     gATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGgtttttt                                                                                                                                                 >  2:1686083/1‑150 (MQ=255)
      aTGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTa                                                                                                                                                >  1:3500961/1‑150 (MQ=255)
        gACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATg                                                                                                                                              <  1:3161174/150‑1 (MQ=255)
         aCGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTAttg                                                                                                                                             >  2:583819/1‑148 (MQ=255)
          cGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTggg                                                                                                                                            >  1:109935/1‑150 (MQ=255)
             tATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCAc                                                                                                                                         <  2:2897000/150‑1 (MQ=255)
              aTTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACg                                                                                                                                        >  1:2660515/1‑150 (MQ=255)
               ttCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTggggccgg                                                                                                                                       >  2:1966552/1‑145 (MQ=255)
                tCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGt                                                                                                                                      <  1:3987357/150‑1 (MQ=255)
                    gctgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCgg                                                                                                                                  >  1:1878734/1‑150 (MQ=255)
                      tgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTTTTGGGAcggggcgggg                                                                                                                                >  2:1235328/1‑150 (MQ=255)
                      tgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCgggg                                                                                                                                <  1:1017549/150‑1 (MQ=255)
                      tgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACTGGGCgggg                                                                                                                                >  2:2149310/1‑150 (MQ=255)
                       gTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGGACGGTGCGGGGt                                                                                                                               >  2:711444/1‑150 (MQ=255)
                         tCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGCATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATTGGCACGGGGCGGGGGGa                                                                                                                             >  1:2154981/1‑150 (MQ=255)
                          cccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTTGGGACGGGGCGGGGGGAg                                                                                                                            >  2:1758860/1‑150 (MQ=255)
                          cccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAg                                                                                                                            >  1:2232721/1‑150 (MQ=255)
                           ccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGt                                                                                                                           <  1:713268/150‑1 (MQ=255)
                            cGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGtt                                                                                                                          <  1:2397910/150‑1 (MQ=255)
                              ttCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGt                                                                                                                        <  2:278682/150‑1 (MQ=255)
                              ttCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGGGAGTTGt                                                                                                                        >  2:3597131/1‑150 (MQ=255)
                               tctcGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTc                                                                                                                       >  1:2872909/1‑150 (MQ=255)
                                 tcGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCgg                                                                                                                     <  2:3034904/150‑1 (MQ=255)
                                     gTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATgc                                                                                                                 <  1:3576307/150‑1 (MQ=255)
                                      tCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGGGGGTTGTCGGGTgcg                                                                                                                >  2:2174352/1‑150 (MQ=255)
                                       cTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATgcgc                                                                                                               >  2:2295632/1‑150 (MQ=255)
                                       cTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGGGCGGGGGGGGGTTTCGGCTgcgg                                                                                                               >  1:1079300/1‑149 (MQ=255)
                                           tACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTTTGGGCACGGTGCGGGGGGAGTTGTCGGATGcgcctcg                                                                                                           >  1:1337595/1‑150 (MQ=255)
                                              tcaTCTGGGGCTTACCGTTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCTCTAAGCGAATTTTAGTTCACATATACCCTGCTTCGGCGGGGTTTTTTTATGGGCCCGGTGCGGGGTGAGTTGGCGGATGcgcttcgttt                                                                                                        <  2:3751867/149‑1 (MQ=255)
                                               caTCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTa                                                                                                       <  1:451098/150‑1 (MQ=255)
                                               caTCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTa                                                                                                       >  1:566403/1‑150 (MQ=255)
                                                  cTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATcc                                                                                                    <  2:2476440/150‑1 (MQ=255)
                                                   tGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGGACGGGGCGGGGGGAGGTGTCGGATGCGCTTCGCTTATCCg                                                                                                   >  2:1652785/1‑150 (MQ=255)
                                                     gAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCTGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGAc                                                                                                 <  2:3887791/150‑1 (MQ=255)
                                                      aGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGAcc                                                                                                >  1:3896530/1‑150 (MQ=255)
                                                         gTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTTTTGGGACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTTTTCGACCttc                                                                                             >  2:2198164/1‑148 (MQ=255)
                                                          tACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACa                                                                                            >  1:3685353/1‑150 (MQ=255)
                                                          tACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGGCCTACa                                                                                            >  2:439390/1‑150 (MQ=255)
                                                              ggTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAgggg                                                                                        >  1:2300936/1‑150 (MQ=255)
                                                               gTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGGGCTTCGCTTATCCGACCTACAGGgga                                                                                       >  1:3345974/1‑150 (MQ=255)
                                                                 ggAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGggagg                                                                                     >  1:2479663/1‑150 (MQ=255)
                                                                  gAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGGGCTTCGCATATCCGACCTACAGggggggg                                                                                    >  2:3558003/1‑149 (MQ=255)
                                                                  gAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGGCCTACAGggggggg                                                                                    >  2:75078/1‑149 (MQ=255)
                                                                     tATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGGGCTTCGCTTATCCGACCCACAGGGGAGGgtat                                                                                 >  1:1249337/1‑150 (MQ=255)
                                                                      aTCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATAtt                                                                                <  1:2763213/150‑1 (MQ=255)
                                                                      aTCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAAGGGAGGAGAtt                                                                                >  2:2572818/1‑150 (MQ=255)
                                                                       tCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTCATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTg                                                                               <  2:3126788/150‑1 (MQ=255)
                                                                           acacGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTTGGGACGGGGGGGGGGGGGTTGTCGGATGCGCTTCGCTTATCCGGCCTACAGGGGAGGAGATTGTAgg                                                                           >  2:617868/1‑150 (MQ=255)
                                                                            cacGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGc                                                                          <  1:4105372/150‑1 (MQ=255)
                                                                             acGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGcc                                                                         <  1:2213836/150‑1 (MQ=255)
                                                                             acGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTATTGTAGGcc                                                                         >  2:384127/1‑150 (MQ=255)
                                                                                tAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCgg                                                                      <  2:3532945/150‑1 (MQ=255)
                                                                                  aGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTa                                                                    <  1:3788916/150‑1 (MQ=255)
                                                                                   gTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTaa                                                                   <  2:1894959/150‑1 (MQ=255)
                                                                                   gTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGGaa                                                                   >  1:2034203/1‑150 (MQ=255)
                                                                                    tACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGTgaa                                                                  >  2:276147/1‑150 (MQ=255)
                                                                                       cAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACgt                                                               <  2:2285544/150‑1 (MQ=255)
                                                                                        aGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACgtg                                                              <  1:1601747/150‑1 (MQ=255)
                                                                                           tGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACgtggtg                                                           <  1:192097/150‑1 (MQ=255)
                                                                                             gTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGcc                                                         <  2:3372650/150‑1 (MQ=255)
                                                                                               gcgcgcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCAt                                                       <  1:2742594/150‑1 (MQ=255)
                                                                                                  cgcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCgg                                                    <  2:2044718/150‑1 (MQ=255)
                                                                                                   gcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCAGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCggg                                                   >  1:1446863/1‑150 (MQ=255)
                                                                                                    cTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGTGGGTTGTCGGATGCGCTTCGCTTTTTCGACCTACAGGGGGGGGTATTGTAGGCCCGGTAAAAGTGGTGCCGTCgggg                                                  >  2:1318459/1‑149 (MQ=255)
                                                                                                        tCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGGACGGTGCGGGGGGAGTTGTCGGAGGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCaaaa                                              >  2:3272329/1‑150 (MQ=255)
                                                                                                        tCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCaaaa                                              <  1:1386246/150‑1 (MQ=255)
                                                                                                          aCTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTTTCCGACCTACAGGGGGGGGTATTGTAGGCCCGGTAAACGTGGGGCCATCCGGCAAAAtt                                            >  2:1560897/1‑150 (MQ=255)
                                                                                                             aaGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGGACGGTGCGGGGGGAGGTGTCGGGTGCGCTTCGCTTTTCCGACCTACAGGGGGGGGTATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGc                                         >  2:2840789/1‑150 (MQ=255)
                                                                                                             aaGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGGTGCGCTTCGCTTATCCGACCTACAGGGGGGGATATTTTAGGCCCGGGAAACGTGGGGCCCTCGGGGAAAATTAGc                                         >  2:2981205/1‑150 (MQ=255)
                                                                                                             aaGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGc                                         >  1:4008264/1‑150 (MQ=255)
                                                                                                              aGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCt                                        >  1:1522062/1‑150 (MQ=255)
                                                                                                                 gAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCgg                                     <  1:1182457/150‑1 (MQ=255)
                                                                                                                 gAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGGGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGGAAAAGTGGGGCCATCGGGCAAAATTAACTCgg                                     >  1:1123291/1‑150 (MQ=255)
                                                                                                                 gAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCgg                                     >  1:758031/1‑150 (MQ=255)
                                                                                                                  aaTTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTATTGTAGGCCCGGTAAACGTGGGGCCATCGGGCAAAATTAGCTCggg                                    >  2:1828422/1‑149 (MQ=255)
                                                                                                                   aTTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCAt                                                       <  2:1066120/130‑1 (MQ=255)
                                                                                                                    ttttAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGt                                  >  1:3610809/1‑150 (MQ=255)
                                                                                                                     tttAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTa                                 <  1:3201261/150‑1 (MQ=255)
                                                                                                                        aGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGGAACa                              >  2:3248194/1‑150 (MQ=255)
                                                                                                                             acaTAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGTGAGTTGTCGGGTGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGGAAACGTGGTGCCATCGGGGAAAATTAGCTCGGAGTAACAGGttt                         >  2:3315510/1‑150 (MQ=255)
                                                                                                                                tAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGttttta                      >  1:48818/1‑148 (MQ=255)
                                                                                                                                tAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGa                      <  1:991820/150‑1 (MQ=255)
                                                                                                                                tAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGa                      <  1:2531768/150‑1 (MQ=255)
                                                                                                                                tAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGGACGGGGGGGGGGGGGGTGTCGGGGGGGCTTCGCTTATTCGACCTACAGGGGGGGGGATTGTAGGGCCGGGAAACGGGGGGCCATCGGGCAAAATTAGGTCGGGGGAAAAGGGTTTgg                      >  2:3105597/1‑149 (MQ=255)
                                                                                                                                tAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGGTGTCGGATGCGCTTCGCTTTTCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGGGTAACAGGgtttta                      >  2:406667/1‑148 (MQ=255)
                                                                                                                                 aGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGat                     <  1:2373007/150‑1 (MQ=255)
                                                                                                                                  gACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGatt                    >  1:168173/1‑150 (MQ=255)
                                                                                                                                   aCCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGatta                   <  1:947211/150‑1 (MQ=255)
                                                                                                                                   aCCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGGAAAATTAGCTCGGAGGAACAGGGTTTGatta                   >  1:2649361/1‑150 (MQ=255)
                                                                                                                                     ccTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGGAAACGGGGTGCCATCGGGCAAAATTAGCTCGGGGGAACAGGGTTTGattatt                 >  2:478238/1‑150 (MQ=255)
                                                                                                                                      cTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTAttt                <  1:1772512/150‑1 (MQ=255)
                                                                                                                                            cggcggGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGaa          <  2:691877/150‑1 (MQ=255)
                                                                                                                                             ggcggGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGGTTTGATTATTTAAGGAAg         >  2:2531098/1‑150 (MQ=255)
                                                                                                                                                ggggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGa      >  2:2487518/1‑150 (MQ=255)
                                                                                                                                                ggggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGa      >  2:1456630/1‑150 (MQ=255)
                                                                                                                                                ggggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGa      >  2:849570/1‑150 (MQ=255)
                                                                                                                                                 gggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGAt     >  2:2914694/1‑150 (MQ=255)
                                                                                                                                                    tttttttATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGAtggt  <  1:2935298/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGT  >  AP012306/1091844‑1092141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑