Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,857,9110AC22.2% 523.8 / 12.5 189K180N (AAA→AAC) aaeRpredicted DNA‑binding transcriptional regulator, efflux system
Reads supporting (aligned to +/- strand):  ref base A (122/25);  new base C (0/42);  total (122/67)
Fisher's exact test for biased strand distribution p-value = 7.97e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 237 total aligned reads displayed.

GCCCCCGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCT  >  AP012306/2857762‑2858060
                                                                                                                                                      |                                                                                                                                                       
 ccccgGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑c                                                                                                                                                       <  1:4109489/150‑1 (MQ=255)
   cccGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑c‑cc                                                                                                                                                    >  1:1208128/1‑150 (MQ=255)
     cGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑ccgc                                                                                                                                                  <  2:1776355/150‑1 (MQ=255)
       aCCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑ccgccg                                                                                                                                                >  1:3263706/1‑150 (MQ=255)
         cTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGAt                                                                                                                                              >  1:3743848/1‑150 (MQ=255)
         cTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGAt                                                                                                                                              >  1:1020380/1‑150 (MQ=255)
               gCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGagt                                                                                                                                        >  1:1346473/1‑150 (MQ=255)
                     gttCTGGGTGGGGTGCCCCGCGGCGGCGCGTCACAGGTTCCCAGCCTTTTCCCCCGCCGTCTGGGCGCGATCAAAAGGGTGG‑TGTGCCCCGCGAAAAGTTATCCCCCAAAACAGGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGtcat                                                                                                                                  <  2:2678181/148‑1 (MQ=255)
                     ggTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGtcat                                                                                                                                  >  2:2882316/1‑150 (MQ=255)
                       tCTGGATGTGGTGATCCGGGTCGGCGCGTTGCAGGATCCCGGCTTGTTTCCCCGCCGTCGGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCAAAAAGCTATCTCCCACAATCCGGCATCCCGAAAAAC‑C‑CCGCCGATTTGAGTAGtcattc                                                                                                                                <  2:3197507/150‑1 (MQ=255)
                          ggATGGGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATg                                                                                                                             >  2:1618775/1‑150 (MQ=255)
                           gATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑A‑CCGCCGATTTGAGTAGTCATTCATgg                                                                                                                            >  2:646780/1‑150 (MQ=255)
                            aTGTGGTGATCCGCGTCGGCGCGTCGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGc                                                                                                                           >  2:192071/1‑150 (MQ=255)
                                ggtgATCCGCGTCGGCGCGTTGAAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGa                                                                                                                       >  2:2619465/1‑150 (MQ=255)
                                   gATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATa                                                                                                                    >  2:1517634/1‑150 (MQ=255)
                                             ggCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCgg                                                                                                          >  2:1604669/1‑150 (MQ=255)
                                               ggggTTGCAGGTTTCCGGCCTTTTTCCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATAGGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGcc                                                                                                        <  2:1434597/147‑1 (MQ=255)
                                               cgcgTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGcc                                                                                                        >  2:2706518/1‑150 (MQ=255)
                                                gcgTTGCAGGTTCCCAGCCTGTTTCCCCGCCGTCGGGGCGCGATGCCAAGGGTGG‑TGTGCGCCGCGAAAATCTATCTCACACAATGCGGCATCCCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGccc                                                                                                       <  2:2208857/150‑1 (MQ=255)
                                                 cgTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCg                                                                                                      >  2:748264/1‑150 (MQ=255)
                                                    tGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACa                                                                                                   >  1:2061664/1‑150 (MQ=255)
                                                     gCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACaa                                                                                                  >  1:1749078/1‑150 (MQ=255)
                                                      cAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAAt                                                                                                 >  1:3689939/1‑150 (MQ=255)
                                                      cAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAAt                                                                                                 >  1:1448268/1‑150 (MQ=255)
                                                        ggATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGa                                                                                               >  2:1688749/1‑150 (MQ=255)
                                                         gttcccAGCCTGTTTCCCCCCCGTCGGGGCGCGATGCAAAGGGGGG‑TGTGCGCCGCAAAAAGCTATCCCACAAAATCCGGCA‑ACCGGAAAAACC‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGaa                                                                                              <  2:1724390/146‑1 (MQ=255)
                                                           ttCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAAtt                                                                                            >  1:1171060/1‑150 (MQ=255)
                                                             ccAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTg                                                                                          >  1:2464088/1‑150 (MQ=255)
                                                             ccAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTg                                                                                          >  1:1631084/1‑150 (MQ=255)
                                                                gcttGTTTCCCCGCCGTCGGGGCGCGATGACAATGGTGG‑TGGGCGCCGCAAAAAGTTATCTCACAAAATCCGGCATACGGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAAc                                                                                       <  2:1093932/147‑1 (MQ=255)
                                                                  cTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTg                                                                                     >  2:2283773/1‑150 (MQ=255)
                                                                   tGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGa                                                                                    >  1:3964602/1‑150 (MQ=255)
                                                                    gTTTCCCCGCCGTCTGGGCGCGATGCAAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATCCCGAAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGAt                                                                                   <  1:2227353/150‑1 (MQ=255)
                                                                          cccccgTCTGGGCGGGATGCAAAGGGGGG‑TGTGCCCCGAAAAAACCTATCCCCCAAAATACGGCAT‑CCGGAAAAACC‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCAcc                                                                             <  2:3490154/147‑1 (MQ=255)
                                                                               gTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAg                                                                        >  1:1041261/1‑150 (MQ=255)
                                                                                gcggggCGGGAT‑CCAATGGGGGTTGCGCGCCGAAAAAACTTTCCCCACACACTGCGGCAGACGGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAgg                                                                       <  2:867995/147‑1 (MQ=255)
                                                                                    ggCGCGATGCCAAGGGTGG‑TGTGCGCCGCAAAAAGCTATCTCACACAATACGGCATACCGGAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGAtc                                                                   <  1:2755132/150‑1 (MQ=255)
                                                                                      ggcgATGCCAATGGTGG‑TGGGCGCCGGAAAAAGCTATCTCACACAATCCGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGAtctc                                                                 <  1:614843/149‑1 (MQ=255)
                                                                                       gcgATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCg                                                                >  1:231177/1‑150 (MQ=255)
                                                                                          aTGCCAATGGTGG‑GGTGCGCCGCAAAAAGCTATCTCACAAAATACGGCATCCCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACt                                                             <  1:3652586/150‑1 (MQ=255)
                                                                                              catgggtgg‑tGTGCGCGGCAAAAAGCTATCTCACACAATACGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGcc                                                         <  1:1874476/146‑1 (MQ=255)
                                                                                              cAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGcc                                                         >  1:3616155/1‑150 (MQ=255)
                                                                                               agtggtgg‑TGTGCGCCGAGAAAACCTACCTCACAAAATACGACATCCCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCt                                                        <  2:1578403/148‑1 (MQ=255)
                                                                                                 tggtgg‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGa                                                      >  1:1132040/1‑150 (MQ=255)
                                                                                                 tggtgg‑TGTGCCCCGAGAAAAGCTATCTCACACAATACGGCATACCGAAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGa                                                      <  1:3483537/150‑1 (MQ=255)
                                                                                                 gggggg‑tgTGCGCCGAAAAAAGCTATCTCACACAATACGGCATCCCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGa                                                      <  2:3719946/146‑1 (MQ=255)
                                                                                                  ggtgg‑tGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGAt                                                     >  1:1300255/1‑150 (MQ=255)
                                                                                                  ggtgg‑tGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGAt                                                     >  1:3431307/1‑150 (MQ=255)
                                                                                                   gtgg‑tgTGCGCGGCGAAAAGCTATCTAACACAATCCGCCATACCGAAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATc                                                    <  1:802757/150‑1 (MQ=255)
                                                                                                   gtgg‑tgTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATc                                                    >  2:1448207/1‑150 (MQ=255)
                                                                                                   gtgg‑tgTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATc                                                    >  2:3371415/1‑150 (MQ=255)
                                                                                                    tgg‑tgTGCGCCGCGAAAAGCTATCTCACACAATACGGCAT‑CCGGAAAAACC‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATcc                                                   <  1:763403/150‑1 (MQ=255)
                                                                                                    tgg‑tgTGCGCCGCAAAAAGCTATCTCACACAATACGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATcc                                                   <  1:499548/150‑1 (MQ=255)
                                                                                                      g‑tgtGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCca                                                 >  2:3884001/1‑150 (MQ=255)
                                                                                                      g‑tgaGCGCGGCGAAGATCTTTCTCAAACAAGCCGAACTAACAAAAACA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCca                                                 <  2:1427570/150‑1 (MQ=255)
                                                                                                        tgtgCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCcac                                                >  1:3178315/1‑150 (MQ=255)
                                                                                                         gtgCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCcaca                                               >  2:494050/1‑150 (MQ=255)
                                                                                                         gtgCGCCGCAAAAAGCTATCTCACAAAATACGGCATACCGAAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCcaca                                               <  1:3940911/150‑1 (MQ=255)
                                                                                                            cgaggtgAAAGGCTATCCCACCGAACAGGGCAT‑CGGGAAAAAGC‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAgg                                            <  1:132915/144‑1 (MQ=255)
                                                                                                              ccggaaaaaGTTATCCCACAAAATGCGGCATCCCGGAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGaa                                          <  1:1070147/145‑1 (MQ=255)
                                                                                                              ccgaaaaaaGCTACCTCACACAATACGGCATACCGGAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGaa                                          <  2:1299390/145‑1 (MQ=255)
                                                                                                                   aaaaGCTATCACACACAATACGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGAttt                                     <  1:877193/150‑1 (MQ=255)
                                                                                                                       gCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGa                                 >  2:3795394/1‑150 (MQ=255)
                                                                                                                          accccacacaATCCGGCAT‑CCGGAAAAACC‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTa                              <  1:3045939/146‑1 (MQ=255)
                                                                                                                          aTCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTa                              >  2:1999849/1‑150 (MQ=255)
                                                                                                                          aTCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTa                              >  1:2344315/1‑150 (MQ=255)
                                                                                                                           tctcACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTaa                             >  2:201103/1‑150 (MQ=255)
                                                                                                                           tctcACACAATACGGAATACGGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTaa                             <  1:2702544/150‑1 (MQ=255)
                                                                                                                           tctcACACAACCGGGCACACGGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTaa                             <  1:4095189/150‑1 (MQ=255)
                                                                                                                            ctcACACAATGCGCCATACCGAAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTAGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAAt                            <  1:2645700/150‑1 (MQ=255)
                                                                                                                             tcACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATg                           >  1:3860974/1‑150 (MQ=255)
                                                                                                                             tcACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATg                           >  1:3223571/1‑150 (MQ=255)
                                                                                                                                 acaATACGGCATACCGGAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATcc                       <  1:1426412/150‑1 (MQ=255)
                                                                                                                                 acaATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATcc                       >  2:3523317/1‑150 (MQ=255)
                                                                                                                                 aaaaTACGCCATACGGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATcc                       <  2:1819605/148‑1 (MQ=255)
                                                                                                                                  caATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCg                      >  1:3513496/1‑150 (MQ=255)
                                                                                                                                  caATACGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCg                      <  1:2933584/150‑1 (MQ=255)
                                                                                                                                  aaaTACGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCg                      <  2:1133627/149‑1 (MQ=255)
                                                                                                                                      ccGGCATACGGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGa                  <  1:2428245/149‑1 (MQ=255)
                                                                                                                                      aCGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTTGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGa                  <  1:401178/150‑1 (MQ=255)
                                                                                                                                      aCGGCATACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGa                  <  2:1205096/150‑1 (MQ=255)
                                                                                                                                        ggCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACg                >  2:3977733/1‑150 (MQ=255)
                                                                                                                                          cATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGGGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCt              >  2:3161550/1‑150 (MQ=255)
                                                                                                                                           aTACCGGAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTg             <  1:3590041/150‑1 (MQ=255)
                                                                                                                                            tACCGAAAAAC‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTgg            <  1:716321/150‑1 (MQ=255)
                                                                                                                                             gcctgAAAAA‑CTCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTgg            <  1:958283/146‑1 (MQ=255)
                                                                                                                                             aCCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGt           >  2:2919240/1‑150 (MQ=255)
                                                                                                                                             aCCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGt           >  1:1788693/1‑150 (MQ=255)
                                                                                                                                             aCCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAAAACAGCGTGCCGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGAGTCGCCTGATCCCAAAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGt           >  2:3480678/1‑150 (MQ=255)
                                                                                                                                               cgaaaaac‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTgc         <  2:2124452/147‑1 (MQ=255)
                                                                                                                                                ggAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTgcg        >  1:1714823/1‑150 (MQ=255)
                                                                                                                                                ggAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTgcg        >  1:1999927/1‑150 (MQ=255)
                                                                                                                                                aaaaaat‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTgcg        <  2:3631817/148‑1 (MQ=255)
                                                                                                                                                 gAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATTATCCGATGACGCTGGTgcgc       >  2:2302765/1‑150 (MQ=255)
                                                                                                                                                  aaaaa‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgct      >  1:2523689/1‑150 (MQ=255)
                                                                                                                                                  aaaaa‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgct      >  1:2976998/1‑150 (MQ=255)
                                                                                                                                                  aaaaa‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgct      >  1:172654/1‑150 (MQ=255)
                                                                                                                                                   aaac‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgctg     <  2:4135023/150‑1 (MQ=255)
                                                                                                                                                      a‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgctggct  >  1:69503/1‑150 (MQ=255)
                                                                                                                                                      a‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCgctggct  >  1:3342960/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GCCCCCGACCTGATTGCCGACGGTCTGGATGTGGTGATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCGATGCCAATGGTGG‑TGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAA‑C‑CCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCT  >  AP012306/2857762‑2858060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑