Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT37.4% 277.0 / 169.5 206S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (12/117);  new base T (77/0);  total (89/117)
Fisher's exact test for biased strand distribution p-value = 3.08e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 240 total aligned reads displayed.

ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGA  >  AP012306/3938738‑3939034
                                                                                                                                                     |                                                                                                                                                     
 cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttct                                                                                                                                                      <  2:2000491/150‑1 (MQ=255)
 cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttct                                                                                                                                                      <  2:1875775/150‑1 (MQ=255)
  tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTtttttttt                                                                                                                                                     >  1:1763602/1‑150 (MQ=255)
  tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                                     >  2:234732/1‑150 (MQ=255)
   cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttt                                                                                                                                                    <  1:265623/150‑1 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                  <  1:3250325/150‑1 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                  <  1:2309819/150‑1 (MQ=255)
      aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGAttttttttttttt                                                                                                                                                 >  2:1648422/1‑149 (MQ=255)
      aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                 <  1:16737/150‑1 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGc                                                                                                                                              <  2:728489/150‑1 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGc                                                                                                                                              <  2:3732931/150‑1 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGc                                                                                                                                              <  1:837591/150‑1 (MQ=255)
         aaaaNGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGGAtttttttttttttttg                                                                                                                                              >  1:2504047/1‑146 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttc                                                                                                                                                       <  2:1412667/140‑1 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttc                                                                                                                                                       >  1:1412667/1‑140 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                                                                                             <  2:1605684/150‑1 (MQ=255)
           aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCttttttttttttttttca                                                                                                                                            >  2:1015297/1‑144 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttgcct                                                                                                                                           >  2:2160552/1‑143 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCAt                                                                                                                                           >  1:1132800/1‑150 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCAt                                                                                                                                           <  1:3971631/150‑1 (MQ=255)
               cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:228793/150‑1 (MQ=255)
               cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:3280379/150‑1 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                                                                      <  2:1091371/150‑1 (MQ=255)
                  cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAg                                                                                                                                     >  1:182405/1‑150 (MQ=255)
                  cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGCAg                                                                                                                                     >  1:646367/1‑150 (MQ=255)
                  cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                                     <  2:2970091/150‑1 (MQ=255)
                   aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAGt                                                                                                                                    >  1:540192/1‑150 (MQ=255)
                     gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGa                                                                                                                                  >  2:3592866/1‑150 (MQ=255)
                      agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                                 <  1:2878521/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                               <  2:1259349/150‑1 (MQ=255)
                         agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCGTTTTTCTTTTTTTTGCAGGAGAAATGGgcgc                                                                                                                              >  1:190245/1‑150 (MQ=255)
                          gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                             <  1:219824/150‑1 (MQ=255)
                           taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGAAGTGAGGGCag                                                                                                                            >  2:3991968/1‑150 (MQ=255)
                           taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTTTTCCCTGAGAAGGGAgcgccg                                                                                                                            >  2:130703/1‑148 (MQ=255)
                           taTCGGTATTGACAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCCCCATGCCTATACCAGTCGGGAGTTATTTCAGGAGGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                            <  2:3916526/150‑1 (MQ=255)
                            aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAgcgccgg                                                                                                                           >  1:746227/1‑147 (MQ=255)
                            aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTCAGAAGAGAGCGgaga                                                                                                                           >  2:3398605/1‑150 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                          <  1:3139473/150‑1 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                          <  2:1220097/150‑1 (MQ=255)
                                gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAgg                                                                                                                       >  2:1477264/1‑150 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                                      <  1:1992368/150‑1 (MQ=255)
                                    tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGGGAGGGCAGAGAgggggg                                                                                                                   >  2:3062250/1‑147 (MQ=255)
                                     gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                  <  1:1391321/150‑1 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  2:2812885/150‑1 (MQ=255)
                                       gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                                <  1:3023441/150‑1 (MQ=255)
                                        agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTTCATTAGAAGTGAGGGCAGgggggggattttt                                                                                                               >  2:4060554/1‑143 (MQ=255)
                                         gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTGAGGAGTGAGCGCAGAGAGGGGTTATCtt                                                                                                              >  2:2736356/1‑150 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGAAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTTTGGCCTCAGAAGTGGGGGCGAAGAGGGAGTATATTg                                                                                                             >  2:1372652/1‑150 (MQ=255)
                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGTGAAGTGGGCGGAGAGAGGGGTTAtttttt                                                                                                            >  2:1731134/1‑148 (MQ=255)
                                             aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTGCCTGAGGAGTGAGGGCAGAGAGGGGTTATACTGCc‑t                                                                                                         >  1:4128684/1‑150 (MQ=255)
                                             aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCc‑t                                                                                                         <  1:3049966/150‑1 (MQ=255)
                                                cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCc‑tgac                                                                                                      <  1:3859574/150‑1 (MQ=255)
                                                   aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGGGAAGTGAGCGCCGAGAGGGAGTATCTTTTc‑tgac‑tga                                                                                                  >  1:2871394/1‑150 (MQ=255)
                                                   aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCc‑tgac‑tga                                                                                                  <  1:3154821/150‑1 (MQ=255)
                                                   aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTTCCTTAGAAGGGAGCGGCGGGGGGGGTTATTTTTCc‑tggtttg                                                                                                   >  2:15481/1‑145 (MQ=255)
                                                    aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGGTTATTTTGCC‑TGAC‑CGaa                                                                                                 >  1:665023/1‑150 (MQ=255)
                                                    aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGGTATCTTTCC‑TGTC‑TGaa                                                                                                 >  1:22933/1‑150 (MQ=255)
                                                      gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACt                                                                                               <  2:3106939/150‑1 (MQ=255)
                                                       tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTg                                                                                              <  1:9691/150‑1 (MQ=255)
                                                         tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTGCATTGGAAGTGAGCGCCGAGGGGGGTTATTTTGCC‑TGAC‑TGAACTGGc                                                                                            >  1:1083877/1‑150 (MQ=255)
                                                                   ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGttt                                                                                  <  1:2789627/150‑1 (MQ=255)
                                                                     ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCC‑TGTC‑TTAACTGGCGGTTAAGttttc                                                                                >  2:1621693/1‑148 (MQ=255)
                                                                        taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGGAGGGAGGGGTTTTTTTTCC‑TGAC‑TGAACTGGCGGTTAAATTTTcgcg                                                                             >  2:1005429/1‑150 (MQ=255)
                                                                         aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGATTTTTTTTTTTTTTTTCGTGGGGAGGTAGCGGTGGGAGGGGTTATTTTGCC‑TGTCTTGAATAGGGGTTTAAGTTTcgccg                                                                             >  2:607702/1‑146 (MQ=255)
                                                                          aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTa                                                                           <  1:1273459/150‑1 (MQ=255)
                                                                          aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTa                                                                           <  2:2720121/150‑1 (MQ=255)
                                                                             tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTAccc                                                                        <  1:2542365/150‑1 (MQ=255)
                                                                                aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGc                                                                     <  2:3211953/150‑1 (MQ=255)
                                                                                 tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                                    <  2:1821735/150‑1 (MQ=255)
                                                                                  aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt                                                                   <  2:3016122/150‑1 (MQ=255)
                                                                                          cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAt                                                           <  1:3989019/150‑1 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                         <  2:1488205/150‑1 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTACTTTTTTTTTTTTCTGGCCCGGGAAGGGGGGGCAGGGAGGGGGTTTCTTTCCGTGCC‑GGAAACGGCAGTTAGGTTTCCCCTTCCCCGGCGtttgggtctg                                                          >  2:3463140/1‑143 (MQ=255)
                                                                                              cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCCGAGAGGGGGTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTTCCCGTACCCCGCGTTTTGTTAttttg                                                       >  2:3901234/1‑147 (MQ=255)
                                                                                              cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                                       <  2:267288/150‑1 (MQ=255)
                                                                                              cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTTCCCGAGGAGGGAGCGCAGAGAGGGGTTATCTTGTC‑TTGC‑TGAAATGGCGATTAAGTTTCCCCGTACCCCGCGGTTTGTTATTTCg                                                       >  2:2207990/1‑150 (MQ=255)
                                                                                               aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCC‑TGAC‑TTAAATGGCGATTTAGTTTTCGCGTGCCCCGCGGTTTGTTATTTCgg                                                      >  2:2923333/1‑150 (MQ=255)
                                                                                                    tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                                 <  2:674749/150‑1 (MQ=255)
                                                                                                         tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                            <  1:1076742/150‑1 (MQ=255)
                                                                                                          cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCC‑TGAC‑TGAACTGGCGGTTAAGTTTTCCCGTACCCCGCGGTTTGTTATTTGGGATGATGGTAtt                                           >  2:2009402/1‑150 (MQ=255)
                                                                                                          cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                           <  1:3535449/150‑1 (MQ=255)
                                                                                                           aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                          <  2:2116541/150‑1 (MQ=255)
                                                                                                           aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTGCGGAGTGGGCGCCGAGAGGGGGTATCTTGCC‑TGAC‑TGGACCGGCGGTTTAGGTTTCGCGGACCCGGCGCTTAGTTATTGCGGATGATGATATTg                                          >  2:2590666/1‑150 (MQ=255)
                                                                                                            ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGa                                         <  1:297624/150‑1 (MQ=255)
                                                                                                              aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                       <  2:2490442/150‑1 (MQ=255)
                                                                                                                      tCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTg                               <  2:2556940/150‑1 (MQ=255)
                                                                                                                            aTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                         <  1:1582320/150‑1 (MQ=255)
                                                                                                                            aTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                         <  1:1209683/150‑1 (MQ=255)
                                                                                                                             tctcGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGGGGGGGGGGGGGGGGGGTTTCTTTCC‑TGTC‑TGAACTGGCGGTTTAGATTTCCCGGACCCCGCGGTTTGTTATTTTGGGTGGTGAGATTTTAGCGCGGCGGGTTTGtt                        >  2:2843586/1‑150 (MQ=255)
                                                                                                                                 gTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGAGAGAGGGGGGGGGGGGTTTTTTGGC‑TGAC‑TTTACTGGGGGTTAAGTTTTCGCGTGCCCCGCGCTTTGTTATTTTGGATGATGATATTTAAACTCGGGCggttggtttctt                    >  2:2907301/1‑146 (MQ=255)
                                                                                                                                 gTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCAGAGAGGGGTTATTTTTCC‑TGTC‑TGAACTGGGGATTAAGTTTCCGCGTACCCCGCGTTTTGTTATTTCGGATGATGATATTTAAGCTCGGCTGGTTGGTTTAtt                    >  2:498317/1‑150 (MQ=255)
                                                                                                                                 gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt                    <  2:3797018/150‑1 (MQ=255)
                                                                                                                                  tttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg                   <  1:2617340/150‑1 (MQ=255)
                                                                                                                                         cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt            <  1:1318988/150‑1 (MQ=255)
                                                                                                                                            tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc         >  2:3584277/1‑150 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg       <  2:4031636/150‑1 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg       <  1:485226/150‑1 (MQ=255)
                                                                                                                                                tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt     >  1:2029653/1‑150 (MQ=255)
                                                                                                                                                 ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg    <  2:3630879/150‑1 (MQ=255)
                                                                                                                                                  tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg   >  2:3878376/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGAC‑TGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGA  >  AP012306/3938738‑3939034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑