Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 2,938,048 G→A M1M (ATG→ATA) † crp → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,938,0480GA100.0% 1324.8 / NA 340M1M (ATG→ATA) †crpDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (183/157);  total (183/157)

Only 100 of 368 total aligned reads displayed.

GTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGT  >  AP012306/2937901‑2938194
                                                                                                                                                   |                                                                                                                                                  
gTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGt                                                                                                                                                  <  1:2562252/150‑1 (MQ=255)
gTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGt                                                                                                                                                  >  2:16150/1‑150 (MQ=255)
 tACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTg                                                                                                                                                 >  1:1077446/1‑150 (MQ=255)
  aCTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGc                                                                                                                                                <  1:2796088/150‑1 (MQ=255)
   cTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCt                                                                                                                                               <  2:4151912/150‑1 (MQ=255)
    tGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCtt                                                                                                                                              <  1:2031512/150‑1 (MQ=255)
     gCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTg                                                                                                                                             <  2:3678667/150‑1 (MQ=255)
       aTGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGc                                                                                                                                           >  2:1099206/1‑150 (MQ=255)
            tGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAAcc                                                                                                                                      <  1:3331849/150‑1 (MQ=255)
             gCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCg                                                                                                                                     <  1:104805/150‑1 (MQ=255)
                  ggACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAAc                                                                                                                                >  2:3243861/1‑150 (MQ=255)
                     cGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGa                                                                                                                             >  2:219167/1‑150 (MQ=255)
                       tCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGAcc                                                                                                                           <  2:2522960/150‑1 (MQ=255)
                           aTTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGAc                                                                                                                       <  2:1308847/150‑1 (MQ=255)
                            ttACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGAct                                                                                                                      <  1:2270884/150‑1 (MQ=255)
                             tACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGActc                                                                                                                     <  2:3414684/150‑1 (MQ=255)
                               ccGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACtctc                                                                                                                   >  2:74347/1‑150 (MQ=255)
                               ccGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACtctc                                                                                                                   <  1:899401/150‑1 (MQ=255)
                                   ggaGTAAAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGGGGCGTTATCTGGCTCTGGAGAAAGTTGATAACAGGGTATACCCGGGCATAGTGCTTGGCAACCCGCAAAAAGACCCGACTCTCGAAt                                                                                                               <  2:925533/148‑1 (MQ=255)
                                   gCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAAt                                                                                                               <  1:2161366/150‑1 (MQ=255)
                                   gCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAACCATATTCCGGCAATCCAGCGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATACCCGCGCATAGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAAt                                                                                                               <  2:4100800/150‑1 (MQ=255)
                                    cAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATg                                                                                                              >  2:1640132/1‑150 (MQ=255)
                                    cAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATg                                                                                                              >  1:516023/1‑150 (MQ=255)
                                    cAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTCCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATg                                                                                                              <  1:2383381/150‑1 (MQ=255)
                                     aGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATgg                                                                                                             <  2:2297083/150‑1 (MQ=255)
                                      gTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGt                                                                                                            >  1:3769747/1‑150 (MQ=255)
                                       tACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCAGACTCTCGAATGGtt                                                                                                           <  1:2047061/150‑1 (MQ=255)
                                        aCAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTc                                                                                                          <  2:1794526/150‑1 (MQ=255)
                                         cAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCt                                                                                                         <  1:4114442/150‑1 (MQ=255)
                                                tAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCa                                                                                                  >  2:3026151/1‑150 (MQ=255)
                                                tAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCa                                                                                                  >  2:2752865/1‑150 (MQ=255)
                                                  gCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCAtt                                                                                                <  1:3155210/150‑1 (MQ=255)
                                                  gCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCAtt                                                                                                >  1:384491/1‑150 (MQ=255)
                                                  gCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGAATAGTGTTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCAtt                                                                                                <  1:2462737/150‑1 (MQ=255)
                                                    cccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGc                                                                                              >  1:1155299/1‑150 (MQ=255)
                                                      cTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCca                                                                                            <  1:186424/150‑1 (MQ=255)
                                                         cccAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACAt                                                                                         >  2:321853/1‑150 (MQ=255)
                                                          ccAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACAtt                                                                                        >  2:3799830/1‑150 (MQ=255)
                                                            aGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCa                                                                                      >  2:149791/1‑150 (MQ=255)
                                                             ggTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCAt                                                                                     >  1:252264/1‑150 (MQ=255)
                                                                aGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAg                                                                                  <  2:1156962/150‑1 (MQ=255)
                                                                aGCGGGAAGAATATTTCGGAAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAg                                                                                  <  2:1070630/150‑1 (MQ=255)
                                                                  cGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTa                                                                                >  1:1376418/1‑150 (MQ=255)
                                                                    ggAAGCATATTTCGGCAATCCAGAGCCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGTTTGGCAACCCGAAAAAAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTAcc                                                                              <  2:1911480/150‑1 (MQ=255)
                                                                     gAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTAccc                                                                             <  1:2748526/150‑1 (MQ=255)
                                                                      aaGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCa                                                                            <  2:2034813/150‑1 (MQ=255)
                                                                       aGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCAt                                                                           >  1:1706703/1‑150 (MQ=255)
                                                                        gCATATTTCGGCAATCCAGAGACGGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATc                                                                          <  2:4081895/150‑1 (MQ=255)
                                                                        gCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATc                                                                          >  2:3591701/1‑150 (MQ=255)
                                                                        gCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATc                                                                          <  2:2254881/150‑1 (MQ=255)
                                                                        gCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATc                                                                          <  1:3084171/150‑1 (MQ=255)
                                                                               tCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGc                                                                   >  1:573860/1‑150 (MQ=255)
                                                                                 cgCAACCCAGGCACAGCGGTGTTATCGGGCTATGGAGAAAGCTTATAACAGAGGATACCCGCGCATAGGGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAc                                                                 <  2:3719185/149‑1 (MQ=255)
                                                                                  gCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACg                                                                <  1:727106/150‑1 (MQ=255)
                                                                                   cAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGc                                                               >  2:209862/1‑150 (MQ=255)
                                                                                     aTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCtt                                                             <  1:1345111/150‑1 (MQ=255)
                                                                                     aTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCtt                                                             <  2:514562/150‑1 (MQ=255)
                                                                                     aTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCtt                                                             >  1:3948258/1‑150 (MQ=255)
                                                                                          gagaCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCa                                                        <  2:686794/150‑1 (MQ=255)
                                                                                           agaCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAc                                                       >  1:3400019/1‑150 (MQ=255)
                                                                                             aCAGCGGCGTTATCTGCCTCTGGAGAAAGCTTATAACAGAGGATACCCGCCCATCGTGCTTGGCACCCCGCAACCAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa                                                     <  2:811976/150‑1 (MQ=255)
                                                                                              cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                    >  1:1263693/1‑150 (MQ=255)
                                                                                              cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTAAACCAGAGGATAACCGGGCATAGTTCTTGGCAACCCGCAAAAAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                    <  2:3642559/150‑1 (MQ=255)
                                                                                               aGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                   <  1:1409693/150‑1 (MQ=255)
                                                                                                gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGGGCTTGGCAAACCGCAAACGGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg                                                  <  1:814150/150‑1 (MQ=255)
                                                                                                     gTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                             <  1:221664/150‑1 (MQ=255)
                                                                                                      ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                            >  1:3515004/1‑150 (MQ=255)
                                                                                                       tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                           <  1:2666173/150‑1 (MQ=255)
                                                                                                            ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                      >  2:576811/1‑150 (MQ=255)
                                                                                                               tttGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                   <  2:1780964/148‑1 (MQ=255)
                                                                                                               tctGGAGAAAGCTTATAACAGAGGATACCCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                   <  2:1027808/150‑1 (MQ=255)
                                                                                                               tctGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                   >  2:2607860/1‑150 (MQ=255)
                                                                                                                ctGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACg                                  <  2:2498879/150‑1 (MQ=255)
                                                                                                                 tGGAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGc                                 >  1:2551526/1‑150 (MQ=255)
                                                                                                                  ggAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                >  2:1689910/1‑150 (MQ=255)
                                                                                                                  ggAGAAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                <  2:121154/150‑1 (MQ=255)
                                                                                                                   gagaAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                               >  2:2407978/1‑150 (MQ=255)
                                                                                                                   gagaAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                               <  1:3637623/150‑1 (MQ=255)
                                                                                                                     gaAAGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta                             >  1:3200071/1‑150 (MQ=255)
                                                                                                                       aaGCTTATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACCCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtact                           >  2:2922541/1‑150 (MQ=255)
                                                                                                                           ttATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                       >  1:2976840/1‑150 (MQ=255)
                                                                                                                           ttATAACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                       >  1:1955276/1‑150 (MQ=255)
                                                                                                                             ataACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                     >  2:2844430/1‑150 (MQ=255)
                                                                                                                             ataACAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                     <  2:2447614/150‑1 (MQ=255)
                                                                                                                              taACAAAGGATAACCGCCCATATTGTTTAGAAAGCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCCCCAGGGTGAAAAAGCGGAAACGCTGTCCTACATCGt                    <  2:3855691/150‑1 (MQ=255)
                                                                                                                              aaaCAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                    >  1:165830/2‑150 (MQ=255)
                                                                                                                               aaCAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                   >  2:3916820/1‑150 (MQ=255)
                                                                                                                               aaCAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                   >  2:3510825/1‑150 (MQ=255)
                                                                                                                                aCAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                  >  2:2597177/1‑150 (MQ=255)
                                                                                                                                 cAGAGGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa                 <  2:2710599/150‑1 (MQ=255)
                                                                                                                                  agagGATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa                >  1:1479440/1‑150 (MQ=255)
                                                                                                                                      gATAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGct            <  1:676568/150‑1 (MQ=255)
                                                                                                                                       aTAACCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctc           <  1:2286967/150‑1 (MQ=255)
                                                                                                                                        tAACCGCTCATAGTGCTTGGCAAACCGCAAAAAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct          <  2:1403109/150‑1 (MQ=255)
                                                                                                                                         aaCCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGGGAAAAACCGGAACCCCTGACTTACACCTTTAAAGCCTCtg         >  1:3322842/1‑150 (MQ=255)
                                                                                                                                         aaCCGCGCATAGTGCTTGGCAAACCGAAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtg         <  2:639360/150‑1 (MQ=255)
                                                                                                                                          ccggccaaTGGTG‑TTGGGAAACCTCCAACAGACCCGGCTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGGGCCCGCTTATTCCCCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg       <  2:1832819/143‑1 (MQ=255)
                                                                                                                                          aCCGCGCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt        <  1:2093940/150‑1 (MQ=255)
                                                                                                                                            cgcgCATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTgg      >  1:625592/1‑150 (MQ=255)
                                                                                                                                                cATAGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGt  >  2:3533833/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGT  >  AP012306/2937901‑2938194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑