BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,073,848	Δ2 bp	intergenic (+106/+161)	lysV → / ← xapR	tRNA‑Lys/DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,073,847	0	G	.	100.0%	567.4 / NA	125	intergenic (+105/+163)	lysV/xapR	tRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (41/84); total (41/84)
*	AP012306	2,073,848	0	T	.	100.0%	567.4 / NA	125	intergenic (+106/+162)	lysV/xapR	tRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (41/84); total (41/84)

Only 100 of 131 total aligned reads displayed.

CTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073698‑2073987

||

cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCg                                                                                                                                               >  1:368821/1‑150 (MQ=32)

 ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                              >  2:2219912/1‑149 (MQ=14)

 ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                              >  1:1543696/1‑149 (MQ=14)

  tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcg                                                                                                                                             <  1:704819/150‑3 (MQ=11)

                                                                                                    gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGa                                       >  2:92362/1‑150 (MQ=0)

                                                                                                     aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaa                                      >  2:2421649/1‑150 (MQ=2)

                                                                                                     aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaa                                      >  1:1889917/1‑150 (MQ=2)

                                                                                                     aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaa                                      >  1:3483264/1‑150 (MQ=2)

                                                                                                      aCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaaa                                     <  2:4053109/150‑1 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:1206467/150‑1 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  2:2999479/150‑1 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:2385225/150‑1 (MQ=255)

                                                                                                       ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:2371392/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  2:710791/1‑150 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:2883885/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:3276703/150‑1 (MQ=255)

                                                                                                        cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  2:3284014/1‑150 (MQ=255)

                                                                                                           cagcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGcc                                >  1:2248757/1‑150 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  1:3774423/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:394258/150‑1 (MQ=255)

                                                                                                            agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  1:2741851/150‑1 (MQ=255)

                                                                                                             gcagGTTTGAGTTGAGCGTAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  2:108257/150‑1 (MQ=11)

                                                                                                             gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  2:2986253/150‑1 (MQ=255)

                                                                                                             gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              >  1:190000/1‑150 (MQ=255)

                                                                                                              cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGa                             <  2:1816152/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  1:2898978/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  1:4305690/150‑1 (MQ=255)

                                                                                                               agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            >  1:200022/1‑150 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGGAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:3527058/150‑1 (MQ=14)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATTTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:204444/150‑1 (MQ=14)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:1811390/150‑1 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:2744176/150‑1 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:2695366/150‑1 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:1892663/150‑1 (MQ=255)

                                                                                                                ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:2219073/150‑1 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAGGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCTTGATgc                          <  1:1321156/150‑1 (MQ=11)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  2:3944864/150‑1 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  2:1725916/150‑1 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  1:3283532/150‑1 (MQ=255)

                                                                                                                 gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  2:935293/150‑1 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         >  2:2919510/1‑150 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  1:307281/150‑1 (MQ=255)

                                                                                                                  tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         >  1:4132372/1‑150 (MQ=255)

                                                                                                                  gttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  2:1683212/149‑1 (MQ=255)

                                                                                                                   ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        <  2:802884/150‑1 (MQ=255)

                                                                                                                   ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        <  1:858565/150‑1 (MQ=255)

                                                                                                                   ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        <  1:3443355/150‑1 (MQ=255)

                                                                                                                   ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        >  1:3648764/1‑150 (MQ=255)

                                                                                                                    tgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCt                       >  2:398918/1‑150 (MQ=255)

                                                                                                                    tgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCt                       <  2:4048114/150‑1 (MQ=255)

                                                                                                                     gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  1:3162495/150‑1 (MQ=255)

                                                                                                                     gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  1:3207699/150‑1 (MQ=255)

                                                                                                                      agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     >  1:2048417/1‑150 (MQ=255)

                                                                                                                      agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     <  2:1922544/150‑1 (MQ=255)

                                                                                                                      agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     <  2:3880696/150‑1 (MQ=255)

                                                                                                                       gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    <  2:1229883/150‑1 (MQ=255)

                                                                                                                       gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    >  1:908634/1‑150 (MQ=255)

                                                                                                                       gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    <  1:3984924/150‑1 (MQ=255)

                                                                                                                        ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   <  2:1448121/150‑1 (MQ=255)

                                                                                                                         tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  2:2566255/150‑1 (MQ=255)

                                                                                                                         tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  1:1874487/150‑1 (MQ=255)

                                                                                                                          gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  2:2994250/150‑1 (MQ=255)

                                                                                                                            gcgcAGCGTAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  2:2539342/150‑1 (MQ=17)

                                                                                                                            gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  2:1919439/150‑1 (MQ=255)

                                                                                                                            gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:3785648/150‑1 (MQ=255)

                                                                                                                            gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACACACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:2123560/150‑1 (MQ=17)

                                                                                                                             cgcgGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  2:1566621/150‑1 (MQ=17)

                                                                                                                             cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  1:1244978/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              >  1:2025811/1‑150 (MQ=17)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  2:245900/150‑1 (MQ=255)

                                                                                                                              gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             >  2:2567615/1‑150 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  2:2184891/1‑150 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  2:2267601/1‑150 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  2:1835314/1‑150 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  1:3674740/150‑1 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  1:4272616/150‑1 (MQ=255)

                                                                                                                               cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  1:2216292/1‑150 (MQ=255)

                                                                                                                                aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           <  2:2719260/150‑1 (MQ=255)

                                                                                                                                 gCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGac          >  2:2643882/1‑150 (MQ=255)

                                                                                                                                  cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         >  1:1740364/1‑150 (MQ=255)

                                                                                                                                  cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  2:4069397/150‑1 (MQ=255)

                                                                                                                                  cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  2:825679/150‑1 (MQ=255)

                                                                                                                                  cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  1:4075839/150‑1 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  2:598934/1‑150 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  1:1458183/1‑150 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  2:1187058/1‑150 (MQ=255)

                                                                                                                                   gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        <  2:1696964/150‑1 (MQ=255)

                                                                                                                                    aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  1:459738/150‑1 (MQ=255)

                                                                                                                                    aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  2:497666/150‑1 (MQ=255)

                                                                                                                                     aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      >  2:3753913/1‑150 (MQ=255)

                                                                                                                                     aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      >  2:918301/1‑150 (MQ=255)

                                                                                                                                      acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     <  1:3438950/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:4083565/150‑1 (MQ=255)

                                                                                                                                       caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:4297317/150‑1 (MQ=255)

                                                                                                                                        aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:2529849/150‑1 (MQ=255)

                                                                                                                                        aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  2:1707830/150‑1 (MQ=255)

                                                                                                                                        aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:1731442/150‑1 (MQ=255)

                                                                                                                                        aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   >  2:2478144/1‑150 (MQ=255)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  >  2:1602114/1‑150 (MQ=255)

                                                                                                                                         acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  2:878262/150‑1 (MQ=255)

||

CTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073698‑2073987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`