BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,073,851	Δ1 bp	intergenic (+109/+159)	lysV → / ← xapR	tRNA‑Lys/DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,073,851	0	T	.	100.0%	568.2 / NA	125	intergenic (+109/+159)	lysV/xapR	tRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (41/84); total (41/84)

Only 100 of 126 total aligned reads displayed.

GAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073798‑2073987

gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGa                                       >  2:92362/1‑150 (MQ=0)

 aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaa                                      >  2:2421649/1‑150 (MQ=2)

 aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaa                                      >  1:3483264/1‑150 (MQ=2)

 aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaa                                      >  2:404652/1‑150 (MQ=2)

  aCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGaaa                                     <  2:4053109/150‑1 (MQ=255)

   ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:1206467/150‑1 (MQ=255)

   ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    >  1:3739120/1‑150 (MQ=255)

   ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  2:2999479/150‑1 (MQ=255)

   ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:2385225/150‑1 (MQ=255)

   ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:2371392/150‑1 (MQ=255)

   ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAt                                    >  2:3785186/1‑150 (MQ=255)

    cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:3276703/150‑1 (MQ=255)

    cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  2:3284014/1‑150 (MQ=255)

    cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:3072829/150‑1 (MQ=255)

    cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAAtt                                   >  2:710791/1‑150 (MQ=255)

       cagcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGcc                                >  1:4134498/1‑150 (MQ=255)

        agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:394258/150‑1 (MQ=255)

        agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  1:3774423/150‑1 (MQ=255)

        agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:2981711/150‑1 (MQ=255)

        agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  1:2741851/150‑1 (MQ=255)

         gcagGTTTGAGTTGAGCGTAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  2:108257/150‑1 (MQ=11)

         gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              >  1:190000/1‑150 (MQ=255)

         gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  2:2986253/150‑1 (MQ=255)

          cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGa                             <  2:1816152/150‑1 (MQ=255)

          cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGa                             <  2:19512/150‑1 (MQ=255)

           agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  1:2898978/150‑1 (MQ=255)

           agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            >  1:200022/1‑150 (MQ=255)

           agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  1:4305690/150‑1 (MQ=255)

            ggTTTGAGTTGAGCGGAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:3527058/150‑1 (MQ=14)

            ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:1811390/150‑1 (MQ=255)

            ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:2695366/150‑1 (MQ=255)

            ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:1892663/150‑1 (MQ=255)

            ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:2219073/150‑1 (MQ=255)

            ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:2744176/150‑1 (MQ=255)

             gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAGGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCTTGATgc                          <  1:1321156/150‑1 (MQ=11)

             gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  2:1761748/150‑1 (MQ=255)

             gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  2:935293/150‑1 (MQ=255)

             gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  1:3388208/150‑1 (MQ=255)

             gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  1:3283532/150‑1 (MQ=255)

             gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  2:3944864/150‑1 (MQ=255)

              tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         >  1:4132372/1‑150 (MQ=255)

              tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  1:307281/150‑1 (MQ=255)

              gttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  2:1683212/149‑1 (MQ=255)

               ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        >  1:3648764/1‑150 (MQ=255)

               ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        <  1:3443355/150‑1 (MQ=255)

               ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        <  2:802884/150‑1 (MQ=255)

                tgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCt                       >  2:398918/1‑150 (MQ=255)

                 gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  1:3543969/150‑1 (MQ=255)

                 gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  1:3207699/150‑1 (MQ=255)

                  agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     <  2:1922544/150‑1 (MQ=255)

                   gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    <  2:1229883/150‑1 (MQ=255)

                   gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    >  1:908634/1‑150 (MQ=255)

                   gttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCa                    <  1:3984924/150‑1 (MQ=255)

                    ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   <  2:1448121/150‑1 (MQ=255)

                     tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  2:4118470/150‑1 (MQ=255)

                     tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  1:1874487/150‑1 (MQ=255)

                     tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  2:2566255/150‑1 (MQ=255)

                      gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 <  2:2994250/150‑1 (MQ=255)

                        gcgcAGCGTAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  2:2539342/150‑1 (MQ=17)

                        gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:3785648/150‑1 (MQ=255)

                        gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  2:1919439/150‑1 (MQ=255)

                        gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCT‑AAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  2:1042348/150‑1 (MQ=17)

                        gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACACACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:2123560/150‑1 (MQ=17)

                         cgcgGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  2:1566621/150‑1 (MQ=17)

                         cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  1:1244978/150‑1 (MQ=255)

                          gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              >  1:2025811/1‑150 (MQ=17)

                          gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  1:2055304/150‑1 (MQ=255)

                           cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  2:2184891/1‑150 (MQ=255)

                           cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  1:2216292/1‑150 (MQ=255)

                           cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  2:1835314/1‑150 (MQ=255)

                           cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  2:2267601/1‑150 (MQ=255)

                           cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATACTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  2:2232798/1‑150 (MQ=17)

                            aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           <  2:2719260/150‑1 (MQ=255)

                             gCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGac          <  2:1670534/150‑1 (MQ=255)

                             gCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGac          >  2:2643882/1‑150 (MQ=255)

                              cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  2:4069397/150‑1 (MQ=255)

                              cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  1:4075839/150‑1 (MQ=255)

                              cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  2:825679/150‑1 (MQ=255)

                              cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         >  1:1740364/1‑150 (MQ=255)

                               gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        <  2:1696964/150‑1 (MQ=255)

                               gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  2:2568626/1‑150 (MQ=255)

                               gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        >  1:1458183/1‑150 (MQ=255)

                                aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  2:497666/150‑1 (MQ=255)

                                aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  1:459738/150‑1 (MQ=255)

                                 aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      >  2:3753913/1‑150 (MQ=255)

                                 aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      >  1:1861386/1‑150 (MQ=255)

                                 aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      >  2:918301/1‑150 (MQ=255)

                                  acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     >  2:2462202/1‑150 (MQ=255)

                                   caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:1904286/150‑1 (MQ=255)

                                   caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:4083565/150‑1 (MQ=255)

                                   caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:4297317/150‑1 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:1731442/150‑1 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   >  2:1332120/1‑150 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  2:1707830/150‑1 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   >  1:2349309/1‑150 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:2529849/150‑1 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:678621/150‑1 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   >  1:2845705/1‑150 (MQ=255)

                                    aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   >  2:2478144/1‑150 (MQ=255)

                                     acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  2:878262/150‑1 (MQ=255)

GAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073798‑2073987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`