Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,758,9090TG29.0% 444.7 / 20.7 194G33G (GGT→GGG) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (39/98);  new base G (56/0);  total (96/98)
Fisher's exact test for biased strand distribution p-value = 3.71e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058
                                                                                                                                                      |                                                                                                                                                     
aaGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGt                                                                                                                                                       <  1:3857409/150‑1 (MQ=255)
aaGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGt                                                                                                                                                       <  1:2159260/150‑1 (MQ=255)
 aGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGtt                                                                                                                                                      <  1:328509/150‑1 (MQ=255)
      aaCCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTcc                                                                                                                                                 >  2:2322659/1‑150 (MQ=255)
       aCCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCt                                                                                                                                                >  1:2854130/1‑150 (MQ=255)
            aGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCg                                                                                                                                           >  1:3877357/1‑150 (MQ=255)
              cccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGAt                                                                                                                                         >  2:3580481/1‑150 (MQ=255)
               ccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGTg                                                                                                                                        >  1:211968/1‑150 (MQ=255)
                cGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGGGAAGt                                                                                                                                       >  2:684239/1‑150 (MQ=255)
                cGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGt                                                                                                                                       >  1:423123/1‑150 (MQ=255)
                  ttGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTg                                                                                                                                     <  2:875707/150‑1 (MQ=255)
                   tGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGc                                                                                                                                    <  1:3097291/150‑1 (MQ=255)
                   tGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGc                                                                                                                                    <  1:2613247/150‑1 (MQ=255)
                     ccAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCg                                                                                                                                  >  1:1618936/1‑150 (MQ=255)
                      cAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGAGGTTGCCGa                                                                                                                                 >  2:2598620/1‑150 (MQ=255)
                       aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGAt                                                                                                                                >  1:2673452/1‑150 (MQ=255)
                         tAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATcc                                                                                                                              <  1:458466/150‑1 (MQ=255)
                         tAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATcc                                                                                                                              <  2:1789641/150‑1 (MQ=255)
                          aaaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCt                                                                                                                             <  2:2659785/150‑1 (MQ=255)
                             aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGG‑GGTTTTACCTGGGGGGTGTTGCCGGTCCTGaa                                                                                                                          >  2:3794956/1‑150 (MQ=255)
                             aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGaa                                                                                                                          <  2:4043557/150‑1 (MQ=255)
                             aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGaa                                                                                                                          <  2:3557303/150‑1 (MQ=255)
                                 aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGG‑GGTTTTTACTGGGCGATTTTGTCGATCCTTAAAAAg                                                                                                                      >  1:1466769/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTGTCGCTTCTGAAAAAGa                                                                                                                     >  2:2819823/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTGCCGATCCTGCAAAAGa                                                                                                                     >  2:2495756/1‑150 (MQ=255)
                                   gaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGGTGTTGCCGATCCTGAAAAAGAt                                                                                                                    >  2:399888/1‑150 (MQ=255)
                                     agagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGAttt                                                                                                                  <  2:2776145/150‑1 (MQ=255)
                                      gagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTta                                                                                                                 <  1:2214619/150‑1 (MQ=255)
                                       agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTtat                                                                                                                >  1:1837285/1‑150 (MQ=255)
                                       agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTTCCGATCCTGAAAAAGATTtat                                                                                                                >  1:2394938/1‑150 (MQ=255)
                                       agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGCTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGAATtat                                                                                                                >  1:775811/1‑150 (MQ=255)
                                        ggACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTtata                                                                                                               <  1:3007389/150‑1 (MQ=255)
                                        ggACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTtata                                                                                                               <  1:3921629/150‑1 (MQ=255)
                                         gACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTGCCGATCCTTAAAAAGATTTTTaa                                                                                                              >  2:1139678/1‑150 (MQ=255)
                                          aCTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGGTGCCGGTCCTGGAAAAGATTTATaaa                                                                                                             >  2:3059576/1‑149 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTGCCGATCCTGAAAAAAAATTATAAAga                                                                                                           >  2:3009588/1‑150 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGga                                                                                                           >  1:2357376/1‑150 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTCGGCGATGTTGCCGCTCCTGAAAAAGATTTATAAGga                                                                                                           >  1:380514/1‑150 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCCTGGGGATGTTTCCGATCCTGAAAAAGATTTATAAAga                                                                                                           >  2:1542323/1‑150 (MQ=255)
                                             aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTACC‑GGGCGATGTTGCCGGACCTGGAAAAAAATTATAAAgac                                                                                                          >  2:922666/1‑150 (MQ=255)
                                             aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTTTAAGgac                                                                                                          >  1:3514794/1‑150 (MQ=255)
                                                gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGGGATGTTGCCGATCCTGAAAAAGATTTATAAGgacgac                                                                                                       >  2:1625385/1‑150 (MQ=255)
                                                gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGgacgac                                                                                                       >  1:2523326/1‑150 (MQ=255)
                                                gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGCTTTACCTGGGCGGGGATTTCGCTCCTGTGAAAGATTTATaaaaggggc                                                                                                       >  1:1020865/1‑143 (MQ=255)
                                                 cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGGGCTGTTGCCGATCCTGAAAAAAAATTTTAAAGAGGACa                                                                                                      >  2:2173466/1‑150 (MQ=255)
                                                 cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACa                                                                                                      >  1:4105321/1‑150 (MQ=255)
                                                      ggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCg                                                                                                 <  1:19957/150‑1 (MQ=255)
                                                       gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAAAATTATAAAGACGACAAAACgg                                                                                                >  2:674658/1‑150 (MQ=255)
                                                        aTCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGCAAAAGATTTATAAGGACGACAAACCggg                                                                                               >  1:1779492/1‑150 (MQ=255)
                                                             aaaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTTTAAGGACGACAAAACGGGCTTaa                                                                                          >  2:1871519/1‑150 (MQ=255)
                                                                 cAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAgcgc                                                                                      <  1:3950992/150‑1 (MQ=255)
                                                                   gTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTGAAGCgcgg                                                                                    <  1:3675744/150‑1 (MQ=255)
                                                                   gTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAAACGGGCTTTAGCgcgg                                                                                    >  1:155181/1‑150 (MQ=255)
                                                                    tAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTGTTACCTGGGCGGTGTTTCCGGTCCTCAAAAAGAATTATAAGGACGACAAACCGGGCGTAAGCgcggc                                                                                   >  2:320178/1‑150 (MQ=255)
                                                                        aaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGA‑GGCTTTAACTAGGCGGAGTAGCCGAACCTAAAAAAAATTTCTAAGGACGACAAAACAGGGGTAAGCGCGGGGATg                                                                               >  2:3741249/1‑150 (MQ=255)
                                                                           gATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTAACTGGGCGGAGTTGCCGGTCCTGAAAAAAATTTATAAAGGCAACCAAACCGGCTTAAACgcggcggataaa                                                                            >  2:2135986/1‑144 (MQ=255)
                                                                            atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTTGGCGATGTTTCCGATCCTGCAAAAGATTTATAAGGACGACAAACCCGGCTTTAACGCGGCGCTGAAAg                                                                           >  1:2729833/1‑150 (MQ=255)
                                                                            atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAg                                                                           <  1:3404792/150‑1 (MQ=255)
                                                                            atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAg                                                                           <  2:731983/150‑1 (MQ=255)
                                                                            atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCCGGGGGATGTTGCCCGTCCTGTAAAAGAATTTTAAAGGGCACAAACCCGGGTTAAGAGCGGCGATGgaaa                                                                           >  2:4133269/1‑149 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAc                                                                      >  1:3236223/1‑150 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAc                                                                      <  2:2219920/150‑1 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGAGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGCTCCTGAAAAAGATTTTTAAGGGAGACAAACCGGGCTTAAGCGCGGCGATGGAAGATaaa                                                                      >  2:469456/1‑149 (MQ=255)
                                                                                    cGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGaaa                                                                            <  1:707120/141‑1 (MQ=255)
                                                                                      tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCCCCTCCTGAAAAAGATTTATAAGGGCGACAAACCGGGCCTTAGCGCGGCGATGAAAAATAACCTcca                                                                 >  2:520278/1‑148 (MQ=255)
                                                                                      tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGa                                                                 >  1:2277637/1‑150 (MQ=255)
                                                                                          ggCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAAttt                                                             <  1:612915/150‑1 (MQ=255)
                                                                                          ggCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAGCGCGGCGATGGAAGATAACCTCGAAttt                                                             >  1:2953465/1‑150 (MQ=255)
                                                                                           gCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATtta                                                            <  1:3479852/150‑1 (MQ=255)
                                                                                            cTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTGCCGATCCTGAAAAAGATTTTTAAGGTCGACAAACCGGGCTTAAACCCGGCGATGAAAGATAACCTCGAATttat                                                           >  2:3333836/1‑150 (MQ=255)
                                                                                            cTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGGTCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATttat                                                           >  2:83070/1‑150 (MQ=255)
                                                                                              ttcgttcgTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGG‑GGGTTTTTCTGGGGGATGTTGGCGGTCCTGAAAAAGATTTTTAAGGGAGAGAAAACGGGGTTTAGAGCGGGGGTGAAAGAAAACCTCCAAAtttttt                                                         >  2:2035202/1‑149 (MQ=255)
                                                                                              ttcgttcgTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATttatta                                                         <  1:1703473/150‑1 (MQ=255)
                                                                                                cgttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAAt                                                       <  1:87107/150‑1 (MQ=255)
                                                                                                 gttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTTCTGGGCGATGTTGCCGGTCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAACGCGGCGGTGAAAGATAACCTCGAATTTATTAATa                                                      >  2:801835/1‑150 (MQ=255)
                                                                                                      tcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATacccac                                                 <  2:1226005/150‑1 (MQ=255)
                                                                                                            ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGGGGTGTTTCCGCTCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGGAAGATAACCTCGAATTTATTAAAACCCCCCCCAAt                                           >  2:1281943/1‑150 (MQ=255)
                                                                                                               cAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCCGGGCGGTGTTGCCGGTCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGGAAGATAAACTCGAATTTATTAATACCCACCCCAATCTg                                        >  2:102790/1‑150 (MQ=255)
                                                                                                                aaGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGAATTTATTAATAACCACCCGAATCTgg                                       >  2:324810/1‑150 (MQ=255)
                                                                                                                aaGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGGATTTATTAATACCCACCCGAAACTgg                                       >  2:2241271/1‑150 (MQ=255)
                                                                                                                 aGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGt                                      <  2:2889438/150‑1 (MQ=255)
                                                                                                                  gCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTc                                     <  2:1393404/150‑1 (MQ=255)
                                                                                                                     aGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGGGGTGTTGCCGATCCTGAAAAAGATTTATAAAGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGAATTTTTTAATAACCACCCCAATCTGGTCggg                                  >  2:1465093/1‑149 (MQ=255)
                                                                                                                       cTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTTTAAGGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAGATAAACTCGAATTTATTAATACCCACCCGAATCTGGTCGGAtt                                >  2:2947543/1‑150 (MQ=255)
                                                                                                                       cTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCCGGGCGATGTTTTCGGTCCCGAAAAAGATTTATAAGGGCGACAAACCGGGGTTAAGCGCGGCGATGAAAGATAACCTCGAATTTTTTAATACCCACCCCAATCTGGTCGGGtt                                >  2:268649/1‑150 (MQ=255)
                                                                                                                         ttAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTcc                              >  1:307347/1‑150 (MQ=255)
                                                                                                                           aaCTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTTCCGAACCTGAAAAAGATTTATAAAGACGACAAACCGGGGTTAAGCGCGGCGGGGAAAGATAACCTCGAATTTTTTAATACCCACCCGAATCTGGTCGGGTTTCTg                            >  2:3529967/1‑150 (MQ=255)
                                                                                                                            aCTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGa                           <  1:3186870/150‑1 (MQ=255)
                                                                                                                             cTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGAt                          <  1:2791446/150‑1 (MQ=255)
                                                                                                                              tACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTTCCGGTCCTGAAAAAGATTTTTAAAGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATAACCACCCGAAACTGGTCGGGTTCCTGATg                         >  2:173049/1‑150 (MQ=255)
                                                                                                                                   aaGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGtt                    <  1:1089751/150‑1 (MQ=255)
                                                                                                                                      gATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGttatt                 <  1:3364795/150‑1 (MQ=255)
                                                                                                                                      gATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGttatt                 <  1:4049299/150‑1 (MQ=255)
                                                                                                                                       aTGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGttatta                <  1:1266662/150‑1 (MQ=255)
                                                                                                                                           aGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAttt            >  2:387340/1‑150 (MQ=255)
                                                                                                                                                 gc‑ggTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGCATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATgg      >  1:795055/1‑150 (MQ=255)
                                                                                                                                                 gc‑ggTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATgg      >  2:2102640/1‑150 (MQ=255)
                                                                                                                                                     gTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaag   >  2:488680/1‑150 (MQ=255)
                                                                                                                                                     gTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaag   >  2:1081036/1‑150 (MQ=255)
                                                                                                                                                      ttttACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  <  2:1965015/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑