Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306257,1370TG26.7% 367.7 / 13.7 172L496V (TTG→GTG) betTcholine transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base T (85/41);  new base G (46/0);  total (131/41)
Fisher's exact test for biased strand distribution p-value = 4.08e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

CCCCCGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAG  >  AP012306/256988‑257286
                                                                                                                                                     |                                                                                                                                                      
cccccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGt                                                                                                                                                        >  1:1979097/1‑150 (MQ=255)
 ccccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGtt                                                                                                                                                       <  1:2985775/150‑1 (MQ=255)
 ccccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGtt                                                                                                                                                       >  1:168628/1‑150 (MQ=255)
 ccccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGAGATTATGGGGCTGCCGTTCAGCTTTGTTATCTTCTTCGTGATGGCGGGGtt                                                                                                                                                       >  2:2818903/1‑150 (MQ=255)
    cGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGgtgtg                                                                                                                                                    >  2:1278007/1‑149 (MQ=255)
     ggctggctGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGtat                                                                                                                                                   >  2:886402/1‑150 (MQ=255)
      gctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGtata                                                                                                                                                  >  2:1871319/1‑150 (MQ=255)
       ctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGACGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGtgtta                                                                                                                                                 >  2:2452194/1‑146 (MQ=255)
        tggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATaaa                                                                                                                                                <  1:1941551/150‑1 (MQ=255)
        tggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATaaa                                                                                                                                                >  2:966694/1‑150 (MQ=255)
         ggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑t                                                                                                                                              <  2:2238143/150‑1 (MQ=255)
          gctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑tc                                                                                                                                             <  1:1104992/150‑1 (MQ=255)
          gctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑tc                                                                                                                                             <  1:1554680/150‑1 (MQ=255)
           ctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑tct                                                                                                                                            <  2:1018295/150‑1 (MQ=255)
           ctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑tct                                                                                                                                            >  1:35229/1‑150 (MQ=255)
            tgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCCTGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATGAA‑tctc                                                                                                                                           >  2:2975055/1‑150 (MQ=255)
             gcgcGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑Tctct                                                                                                                                          <  2:603915/150‑1 (MQ=255)
             gcgcGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑Tctct                                                                                                                                          <  1:2232983/150‑1 (MQ=255)
             gcgcGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑Tctct                                                                                                                                          <  1:3430239/150‑1 (MQ=255)
               gcgTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGa                                                                                                                                        >  1:2648020/1‑150 (MQ=255)
               gcgTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGa                                                                                                                                        <  1:2606883/150‑1 (MQ=255)
                cgTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGCTGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGGATTAA‑TCTCTGaa                                                                                                                                       >  2:1661817/1‑150 (MQ=255)
                 gTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTTTAAA‑TCTCTGAAg                                                                                                                                      >  2:1597445/1‑150 (MQ=255)
                 gTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAg                                                                                                                                      <  1:908237/150‑1 (MQ=255)
                   cttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGt                                                                                                                                    >  1:3223908/1‑150 (MQ=255)
                        ggTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTagaag                                                                                                                               <  1:3657679/150‑1 (MQ=255)
                        ggTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTagaag                                                                                                                               >  1:2277279/1‑150 (MQ=255)
                          tCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGAt                                                                                                                             >  1:1008712/1‑150 (MQ=255)
                          tCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGAt                                                                                                                             <  1:1508651/150‑1 (MQ=255)
                            ggTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTa                                                                                                                           >  1:346010/1‑150 (MQ=255)
                                gCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTAccgc                                                                                                                       >  2:3239487/1‑150 (MQ=255)
                                 cGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTAccgcc                                                                                                                      <  1:1661067/150‑1 (MQ=255)
                                   aTTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGt                                                                                                                    >  1:467136/1‑150 (MQ=255)
                                        ccTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTTTAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTTAAAg                                                                                                               >  2:302092/1‑150 (MQ=255)
                                        ccTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAg                                                                                                               >  1:2353328/1‑150 (MQ=255)
                                           gctgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGc                                                                                                            >  2:402943/1‑150 (MQ=255)
                                            ctgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑ATTCTTAAAGTAAGAAATTACCGCCGTGAAAGGGcg                                                                                                           >  2:1753430/1‑149 (MQ=255)
                                             tgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCa                                                                                                          >  2:2944446/1‑150 (MQ=255)
                                                  cTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACcgc                                                                                                     >  1:3574162/1‑150 (MQ=255)
                                                    cGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGa                                                                                                   >  1:437499/1‑150 (MQ=255)
                                                     ggCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGAt                                                                                                  >  1:311015/1‑150 (MQ=255)
                                                     ggCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGAt                                                                                                  >  2:2022794/1‑150 (MQ=255)
                                                        aTGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATacc                                                                                               >  2:3712416/1‑150 (MQ=255)
                                                         tGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATaccg                                                                                              >  2:2922142/1‑150 (MQ=255)
                                                               tgaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATaccgcaccgc                                                                                        >  2:2677710/1‑150 (MQ=255)
                                                               tgaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TATCTGAAGGGAGAAAATTACCACCGGGAAAGGGCCAACCGCGATaccccaccgc                                                                                        >  2:3284366/1‑150 (MQ=255)
                                                                 aCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGGGGAAGATTACCGCCGGGAAAGGGCCAACCGCGATACCGCCCCGCGa                                                                                      >  2:1106536/1‑150 (MQ=255)
                                                                  cTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGAGCCAACCGCGATACCGCACCGCGAc                                                                                     >  1:2314902/1‑150 (MQ=255)
                                                                       gggATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTTAAGGGAGAAGATTATCGCCGTGAAAATGTCAACCGGGATACCACGCCGCGAcccccg                                                                                >  2:3359050/1‑146 (MQ=255)
                                                                        ggATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTTTATAAA‑TCTCTGAAGGGAGAAGAATACCGCCGTGAAAAAGCCCACCGCGAAAACCCACCCCGGCCGCTgg                                                                               >  2:1945180/1‑150 (MQ=255)
                                                                         gATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGGGTATAAA‑TCTCTGAAGGAACAAAAATTCCCCCGTGAAAACGCCCCACACGCAACCGCACCGCGAACGCTggg                                                                              >  2:3186920/1‑150 (MQ=255)
                                                                             tCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGGATAAA‑TCTCTGAGGGGAGAAGGTTACCCCCGTGGAAGTGGCAACCGGGGTACCCCACCGCGAACGCTGGGGCtt                                                                          >  2:3107303/1‑150 (MQ=255)
                                                                               cgcgCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCa                                                                        >  1:2058203/1‑150 (MQ=255)
                                                                                  gcTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGGAGAAGATTACCGCCGGGAAAGGGGCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGa                                                                     >  2:3960749/1‑150 (MQ=255)
                                                                                   cTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGAt                                                                    >  2:3380067/1‑150 (MQ=255)
                                                                                      cAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGc                                                                 >  1:1407241/1‑150 (MQ=255)
                                                                                       aaaaCACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGGAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGcc                                                                >  1:1773077/1‑150 (MQ=255)
                                                                                            accacGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGCAGGTAGAAGATTAACGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCCCTGGGGCTTCAGGATCGCCTGAGc                                                           >  2:3672247/1‑150 (MQ=255)
                                                                                             ccacGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCt                                                          >  2:3778509/1‑150 (MQ=255)
                                                                                              cacGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTg                                                         >  2:320020/1‑150 (MQ=255)
                                                                                               acGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTTTAAA‑TTTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGGCAACCTCGCTACCGCACCCCGACCCCTGGGGGCTCCTGTTCGGCCGAGCTgg                                                        >  2:3982078/1‑150 (MQ=255)
                                                                                               acGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTgg                                                        >  1:2689588/1‑150 (MQ=255)
                                                                                                cGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGa                                                       >  2:2049578/1‑150 (MQ=255)
                                                                                                 ggTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGaa                                                      >  1:3956005/1‑150 (MQ=255)
                                                                                                   tGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCCTCAGGATCGCCTGAGCTGGaaaa                                                    >  1:3618388/1‑150 (MQ=255)
                                                                                                      ttATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGGAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAAGATCGCCTGAGCTGGAAAAAAc                                                 >  2:3147466/1‑150 (MQ=255)
                                                                                                      ttATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGGTACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGGTGGAAAAAAc                                                 >  2:2394225/1‑150 (MQ=255)
                                                                                                         tGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGGGCTGGAAAAAACGtc                                              >  2:1512655/1‑150 (MQ=255)
                                                                                                         tGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtc                                              >  2:500045/1‑150 (MQ=255)
                                                                                                          ggggCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtct                                             >  2:2528534/1‑150 (MQ=255)
                                                                                                          ggggCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtct                                             >  2:1164500/1‑150 (MQ=255)
                                                                                                                 ccGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGGAGAAGATTACCGCCGTGAAAGGGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCccgc                                      >  2:804560/1‑150 (MQ=255)
                                                                                                                   gTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCCCGccc                                    >  1:3882466/1‑149 (MQ=255)
                                                                                                                      cAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCtgat                                 >  1:318384/1‑150 (MQ=255)
                                                                                                                       aGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGTAAAAGATTACCCCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGGAAAAACGTCTCTCGCGCCtgatg                                >  2:3599338/1‑150 (MQ=255)
                                                                                                                          tttGTGATCTTCTTCGTGATGGCGGGGTTGTATTAA‑ACTCTCTAGGTAGAAGATTATCGCCGTGAAAGTGACAACCTGGATACCGCCCCGCGACCGGTGGGGGTTTTAGGTTGCCTTCACTTGAAAAAACGACCCGCCCGCCTGTGGAAt                             >  2:1420541/1‑150 (MQ=255)
                                                                                                                          tttGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAAt                             >  1:2155003/1‑150 (MQ=255)
                                                                                                                           ttGTGCTTTTCTGCGGGAGGGGGGGGTTTTAAAAA‑TCTGTGAAGGTAAAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAAtt                            <  1:999795/150‑1 (MQ=255)
                                                                                                                           ttGTGCTCTTGTGCGTGGTGGGGGGGTTGAAAAAC‑TCCCTGAAGGAAGAAAATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAAtt                            <  1:2527818/150‑1 (MQ=255)
                                                                                                                           ttGTGATCTTCTTGGTGGGGGGGGGGTTGTATAAA‑TCTCTGAGGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAAtt                            <  1:672192/150‑1 (MQ=255)
                                                                                                                            tgttttcttctTGGTG‑GGGGGGGGTTTTAAAAATTTTCGGAGGGTAGAATATTGCCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTa                           <  2:3604996/145‑1 (MQ=255)
                                                                                                                             gtgATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTAt                          >  1:2573436/1‑150 (MQ=255)
                                                                                                                             gtgATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTAt                          >  1:1217072/1‑150 (MQ=255)
                                                                                                                                 tcttctTCGTGATGGCGGGGGTGTATAAA‑ACTCTGAAGGTAAAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCCCCGCGACCCCTGGGGCTTCAGGATCCCCTGAGCTGGAAAAAACGCCTCTCGCGCCCGATGAATTATCCgg                      >  2:3571214/1‑150 (MQ=255)
                                                                                                                                  cttcttCGTGATGGCGGGGGTTTATAAA‑TCTTTCAAGGGAGAAGATTAACGCCGTGGAAGTGCCAACCGCGATATCCCACCGCGACCGCTGGGGCTTCAGGGTCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATTCggg                     >  2:1547924/1‑150 (MQ=255)
                                                                                                                                  cttcttCGTGATGGCGGGGGTGTATAAA‑TCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCCCCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCggg                     >  2:3833323/1‑150 (MQ=255)
                                                                                                                                   ttcttcGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGc                    >  2:1394123/1‑150 (MQ=255)
                                                                                                                                      ttcGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAATGCCAACCGCGATACCACCCCGCGACCGCCGGGGCTTCACGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCAcg                 >  1:2798241/1‑150 (MQ=255)
                                                                                                                                      ttcGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAAAAGATTAACCCCGGGGAAGGGCCAACCGCGGTACCGCACCGCGACCCCTGGGGCTTCAGGATGGCCTGAGCTGGGAAAAACGGCTCTCGCGCCTGCTGAATTATCCggggccg                 >  2:1819778/1‑146 (MQ=255)
                                                                                                                                       tcGTGGGGGGGGGTTTGTATAAA‑TCTCAGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCAcgc                <  1:401239/150‑1 (MQ=255)
                                                                                                                                         gTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGt              >  1:1198723/1‑150 (MQ=255)
                                                                                                                                           gATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTa            >  1:2206318/1‑150 (MQ=255)
                                                                                                                                           gATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTa            >  1:595687/1‑150 (MQ=255)
                                                                                                                                           gATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTa            >  1:3365420/1‑150 (MQ=255)
                                                                                                                                            tggggggggTTTTATAAA‑TCTAGGAGGGAAGAAGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTac           <  2:5906/145‑1 (MQ=255)
                                                                                                                                                cGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTa       >  2:4104325/1‑150 (MQ=255)
                                                                                                                                                 ggggTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTaa      >  1:1275539/1‑150 (MQ=255)
                                                                                                                                                 ggggTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTaa      >  1:855724/1‑150 (MQ=255)
                                                                                                                                                  gggTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTaaa     >  2:2663821/1‑150 (MQ=255)
                                                                                                                                                    tttGTATAAT‑TCTCGAAGGGAAGAAGTTTGCCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACa   <  2:2317924/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCCCCGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAA‑TCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAG  >  AP012306/256988‑257286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑