Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,618,527 C→T A1322V (GCG→GTG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,618,5270CT100.0% 1152.8 / NA 279A1322V (GCG→GTG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (160/119);  total (160/119)

Only 100 of 281 total aligned reads displayed.

ACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGC  >  AP012306/3618380‑3618673
                                                                                                                                                   |                                                                                                                                                  
aCTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTgg                                                                                                                                                  >  2:3091489/1‑150 (MQ=255)
  ttCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCa                                                                                                                                                >  2:114376/1‑150 (MQ=255)
  ttCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCa                                                                                                                                                <  1:398439/150‑1 (MQ=255)
   tCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCAt                                                                                                                                               <  2:799947/150‑1 (MQ=255)
    ccTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATc                                                                                                                                              <  2:2515068/150‑1 (MQ=255)
    ccTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATc                                                                                                                                              <  1:3077388/150‑1 (MQ=255)
     cTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCg                                                                                                                                             <  1:945389/150‑1 (MQ=255)
      tGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGt                                                                                                                                            >  2:565307/1‑150 (MQ=255)
      tGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGt                                                                                                                                            <  1:2058387/150‑1 (MQ=255)
        gAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCATTc                                                                                                                                          >  2:3171364/1‑150 (MQ=255)
         aaGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTcc                                                                                                                                         >  1:1956824/1‑150 (MQ=255)
            gCGAACAGGTTGAATACCCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCGCCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAgg                                                                                                                                      <  2:2873613/150‑1 (MQ=255)
             cGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGga                                                                                                                                     <  1:653477/150‑1 (MQ=255)
              gAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGgag                                                                                                                                    >  2:3735690/1‑150 (MQ=255)
                aCAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGAc                                                                                                                                  >  1:2317335/1‑150 (MQ=255)
                 cAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGAcc                                                                                                                                 >  1:2791092/1‑150 (MQ=255)
                    gTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACt                                                                                                                              <  2:276141/150‑1 (MQ=255)
                     ttGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTc                                                                                                                             >  2:3998412/1‑150 (MQ=255)
                      tGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTcg                                                                                                                            <  1:271802/150‑1 (MQ=255)
                      tGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTcg                                                                                                                            >  1:1186238/1‑150 (MQ=255)
                       gAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTcgc                                                                                                                           >  2:1711813/1‑150 (MQ=255)
                        aaTACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTcgcg                                                                                                                          <  2:292697/150‑1 (MQ=255)
                           aCTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGc                                                                                                                       >  1:1222579/1‑150 (MQ=255)
                             tctcGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTg                                                                                                                     >  2:1434088/1‑150 (MQ=255)
                               tcGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGAc                                                                                                                   >  2:2158755/1‑150 (MQ=255)
                                  cgTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGa                                                                                                                <  1:3046342/150‑1 (MQ=255)
                                   gTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGaa                                                                                                               >  2:2657868/1‑150 (MQ=255)
                                        gATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAagcagc                                                                                                          <  2:3413650/150‑1 (MQ=255)
                                           cGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGt                                                                                                       <  2:3542828/150‑1 (MQ=255)
                                             cAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTg                                                                                                     <  1:3179506/150‑1 (MQ=255)
                                              aaaCCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGc                                                                                                    >  2:879160/1‑150 (MQ=255)
                                                aCCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCgg                                                                                                  <  1:766814/150‑1 (MQ=255)
                                                aCCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCgg                                                                                                  <  1:2158595/150‑1 (MQ=255)
                                                aCCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCgg                                                                                                  <  1:3695756/150‑1 (MQ=255)
                                                      aaCTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAAc                                                                                            >  1:3484825/1‑150 (MQ=255)
                                                        cTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAAcgc                                                                                          <  2:468350/150‑1 (MQ=255)
                                                        cTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAAcgc                                                                                          >  2:2633030/1‑150 (MQ=255)
                                                         tGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAAcgcg                                                                                         >  2:3028564/1‑150 (MQ=255)
                                                            aaGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGcgacg                                                                                      >  1:1273096/1‑150 (MQ=255)
                                                            aaGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGcgacg                                                                                      <  2:3577642/150‑1 (MQ=255)
                                                             aGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGcgacga                                                                                     >  2:1840897/1‑150 (MQ=255)
                                                                gAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACt                                                                                  >  2:2603910/1‑150 (MQ=255)
                                                                gAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACt                                                                                  >  1:81329/1‑150 (MQ=255)
                                                                gAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACt                                                                                  >  2:3069380/1‑150 (MQ=255)
                                                                caaCGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCGCCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCTCTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACt                                                                                  <  2:2442685/149‑1 (MQ=255)
                                                                 aaCGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTg                                                                                 >  1:3570300/1‑150 (MQ=255)
                                                                  aCGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTgc                                                                                <  1:3559344/150‑1 (MQ=255)
                                                                   cGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTgcg                                                                               <  2:1626648/150‑1 (MQ=255)
                                                                   cGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTgcg                                                                               >  1:103179/1‑150 (MQ=255)
                                                                       aaaGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGc                                                                           >  2:618022/1‑150 (MQ=255)
                                                                       aaaGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGc                                                                           >  2:2810935/1‑150 (MQ=255)
                                                                       aaaGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGc                                                                           >  2:129165/1‑150 (MQ=255)
                                                                       aaaGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGc                                                                           >  2:2359975/1‑150 (MQ=255)
                                                                               tGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAaga                                                                   >  1:2680251/1‑150 (MQ=255)
                                                                                  aaCTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCATGAAAGAGaa                                                                >  1:2934524/1‑150 (MQ=255)
                                                                                   aCTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAAc                                                               <  1:1637679/150‑1 (MQ=255)
                                                                                    cTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACg                                                              >  1:2280799/1‑150 (MQ=255)
                                                                                    cTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACg                                                              <  2:3479801/150‑1 (MQ=255)
                                                                                       aCTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTa                                                           >  1:1931009/1‑150 (MQ=255)
                                                                                       aCTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTa                                                           <  1:1583848/150‑1 (MQ=255)
                                                                                         tCCCCCCATCTGCTGGGGATCACCAAAACGTCTCTTGCAACCGCGTCCCTCCTCTCCGCGGCCTCGGTCCCGGAGACCACCCGCGGGCTGGCCGCAGCAACCCTTGCGGGCCAACGCGACGAACTGCGCGGCCCGGAAGAGAAAGTTATc                                                         >  2:2025248/1‑150 (MQ=255)
                                                                                          cccGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCg                                                        >  2:2741974/1‑150 (MQ=255)
                                                                                           ccGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGt                                                       <  2:1868592/150‑1 (MQ=255)
                                                                                             gcgATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTgg                                                     >  1:3774131/1‑150 (MQ=255)
                                                                                              cgATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTggg                                                    >  2:1612413/1‑150 (MQ=255)
                                                                                               gATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGgt                                                   <  2:422272/150‑1 (MQ=255)
                                                                                                  ctgctgGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGgtcgt                                                >  1:235334/1‑150 (MQ=255)
                                                                                                   tgctgGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGgtcgtc                                               <  1:3953610/150‑1 (MQ=255)
                                                                                                   tgctgGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGgtcgtc                                               >  1:863696/1‑150 (MQ=255)
                                                                                                    gctgGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCt                                              >  2:2841301/1‑150 (MQ=255)
                                                                                                        ggTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATc                                          <  2:1087647/150‑1 (MQ=255)
                                                                                                          tATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATccc                                        >  2:1932845/1‑150 (MQ=255)
                                                                                                          tATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATccc                                        >  2:3299415/1‑150 (MQ=255)
                                                                                                          tATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATccc                                        <  1:3298338/150‑1 (MQ=255)
                                                                                                           aTCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCg                                       >  1:3589006/1‑150 (MQ=255)
                                                                                                            tCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCgg                                      <  1:171007/150‑1 (MQ=255)
                                                                                                                cAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAgg                                  <  1:1525772/150‑1 (MQ=255)
                                                                                                                 aaaGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGt                                 >  2:1555358/1‑150 (MQ=255)
                                                                                                                   aGCGTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTAc                               >  2:527249/1‑150 (MQ=255)
                                                                                                                      gTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCgg                            >  1:1073756/1‑150 (MQ=255)
                                                                                                                      gTCTCTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCgg                            >  2:3899450/1‑150 (MQ=255)
                                                                                                                       tctcTGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGt                           >  1:3209577/1‑150 (MQ=255)
                                                                                                                           tGGCAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTAcg                       >  2:3629388/1‑150 (MQ=255)
                                                                                                                              cAACCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGGTACGCGt                    >  2:966749/1‑150 (MQ=255)
                                                                                                                               aaCCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTa                   <  2:1366652/150‑1 (MQ=255)
                                                                                                                                aCCGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTac                  <  1:1423971/150‑1 (MQ=255)
                                                                                                                                 ccGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTacc                 >  1:284195/1‑150 (MQ=255)
                                                                                                                                  cGAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTacca                >  2:249496/1‑150 (MQ=255)
                                                                                                                                   gAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTaccac               >  2:2095741/1‑150 (MQ=255)
                                                                                                                                   gAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTaccac               <  1:2473542/150‑1 (MQ=255)
                                                                                                                                   gAGTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACATTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTaccac               >  1:2540932/1‑150 (MQ=255)
                                                                                                                                     gTCCTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTAccacca             <  2:728858/150‑1 (MQ=255)
                                                                                                                                       ccTTCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAgg           <  2:1000074/150‑1 (MQ=255)
                                                                                                                                         ttCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGAt         <  1:4102443/150‑1 (MQ=255)
                                                                                                                                          tCATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATc        <  2:2988095/150‑1 (MQ=255)
                                                                                                                                           cATCTCCGTGGCATCGTTCCAGGAGTCCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCg       <  2:2261205/150‑1 (MQ=255)
                                                                                                                                           cATCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCg       >  1:3417681/1‑150 (MQ=255)
                                                                                                                                            aTCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGt      >  2:3862608/1‑150 (MQ=255)
                                                                                                                                            aTCTCCGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGt      <  1:554283/150‑1 (MQ=255)
                                                                                                                                                ccGTGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGc  >  2:223870/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAACCGCGAACTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGC  >  AP012306/3618380‑3618673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑