Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,212,7530AC29.8% 262.9 / 51.4 121Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (78/7);  new base C (0/36);  total (78/43)
Fisher's exact test for biased strand distribution p-value = 4.17e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 160 total aligned reads displayed.

CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGC  >  AP012306/2212604‑2212902
                                                                                                                                                     |                                                                                                                                                      
cAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATa                                                                                                                                                        >  2:3767248/1‑150 (MQ=255)
cAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATa                                                                                                                                                        >  2:719608/1‑150 (MQ=255)
  aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑cc                                                                                                                                                     <  1:1981893/150‑1 (MQ=255)
     ccGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCa                                                                                                                                                  >  1:3416207/1‑150 (MQ=255)
     ccGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCa                                                                                                                                                  >  2:2233384/1‑150 (MQ=255)
          gTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCAccgcc                                                                                                                                             >  1:674577/1‑150 (MQ=255)
             gCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCgccgag                                                                                                                                          >  1:1278863/1‑150 (MQ=255)
             gCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCgccgag                                                                                                                                          >  2:3729208/1‑150 (MQ=255)
               tCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCgccgaggc                                                                                                                                        >  2:414925/1‑150 (MQ=255)
                 ccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCGCCgccgaggccg                                                                                                                                      >  1:3827925/1‑150 (MQ=255)
                 ccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCgccgaggccg                                                                                                                                      >  1:920592/1‑150 (MQ=255)
                 ccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCgccgaggccg                                                                                                                                      <  1:2752131/150‑1 (MQ=255)
                    tAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGt                                                                                                                                   <  1:3113930/150‑1 (MQ=255)
                        gCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGcc                                                                                                                               >  2:3345495/1‑150 (MQ=255)
                         ccccAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCa                                                                                                                              >  1:712870/1‑150 (MQ=255)
                         ccccAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCAAAAATC‑CCCCACCGCCGAGGCCGAGTAGCCa                                                                                                                              <  1:1346495/150‑1 (MQ=255)
                         ccccAGCCCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCGCCCCCCCGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCa                                                                                                                              <  2:2315045/150‑1 (MQ=255)
                            cAGCGCACTACGCCGCAACTTGCCGCGATCAACAGCATATCGCGTGCACTACAACGGCGGGAAAATTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAAAA‑CCCCCCCGCCGAGGCCGAGTAGGCAGct                                                                                                                           >  2:3052167/1‑150 (MQ=255)
                            cAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCCCGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGct                                                                                                                           <  2:3508917/150‑1 (MQ=255)
                               cgcACTACGCCGCAAACCGCCGAGATCAACAGCATACCGCGTGCACTACACCGGCGAAAAAGTTTATTCCTCAGCGCAAAGATAATGCCTTCCGCCCCCCCGCCCGGCGCCCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGctgcc                                                                                                                        <  2:1621659/150‑1 (MQ=255)
                                  aCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGctgcctgc                                                                                                                     <  1:1436363/150‑1 (MQ=255)
                                    tACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCa                                                                                                                   >  1:3697502/1‑150 (MQ=255)
                                      cgccgaAAACCCCCGAGACCACCAGAATACCGCGGCCACTACAACGGCGAAAAATTTTATTCCCCAGCCCAAAGAAAAGGCCTCCCGCCCCCCCCCCCAGCGCCCCAAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGt                                                                                                                 <  2:1244676/150‑1 (MQ=255)
                                          gcAAATCGCAGAGATAAACAGCATATCGCGTGCACTACAACGGCGGAAAATTTTATTACTCAGCCAAAAGATAATGACTTCCGCCCCCCCGCCCAGCGCCCACAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAAt                                                                                                             <  2:549888/150‑1 (MQ=255)
                                           cAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCCGTAAATg                                                                                                            >  2:1515931/1‑150 (MQ=255)
                                           cAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATg                                                                                                            >  2:1136140/1‑150 (MQ=255)
                                           aaaaCCGCCGAAATCAACAGCATACCGCGTCCACTACAACGGCGAAAAATTTTATTCCCCAGCGAAAAGAAAATGCCTCCCGCCCCCCCGCCCAGCGACCAAAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATg                                                                                                            <  2:2966148/149‑1 (MQ=255)
                                              aTCGCCGAAATCAACAGCATATCGCGTGCACTACAACGGCGAAAAAGTTTATTACCCACCGCAAAGATAATGACTCCCGCCACCCCGCCCAGCGCCCAAAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGgcg                                                                                                         <  2:3909529/150‑1 (MQ=255)
                                                   cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaa                                                                                                    <  1:1530993/150‑1 (MQ=255)
                                                   cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGGAGCCAGCTGCCTGCCAGTAAATGGCGCTaaa                                                                                                    >  2:1710057/1‑150 (MQ=255)
                                                    gagaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                                   >  1:3112253/1‑150 (MQ=255)
                                                    gagaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                                   >  2:907190/1‑150 (MQ=255)
                                                    gagaTCAACAGCATATCGCGGGCCCTACAACGGGGAAAAAGTTTATTACTAAGCGAAAAGATAATGACTCCCCCCCCCCCCCCCAGCGCCCACAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                                   <  2:1199875/150‑1 (MQ=255)
                                                     agaTCAACAGCATATCGCGTGCGCTACAACGGCGGAAAAGTTTATTACTCCGCGCAAAGGTAATGACTTCCGCCGCCACGCCCCGCGGCCAAAAAAA‑CCCCACCGCCGAGGCCGAGCAGCCACCCGCCTGCCCGTAAAAGGCGCTtaacc                                                                                                  >  2:3789022/1‑148 (MQ=255)
                                                     agaTCAACAGCATATCGCGTGCACTACAACGGGGAAAAAGTTTATTACCCAGCGAAAAGAAAAGGCCTCCCGCCCCCACGCCCAGCGCCCAAAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAc                                                                                                  <  2:3392614/150‑1 (MQ=255)
                                                      gaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAcc                                                                                                 >  1:2046133/1‑150 (MQ=255)
                                                      gaTCAACAGAATATCGCGTGCCCTAAAACGGCGAAAAATTTTATTCCTCAGCGCAAAGATAATGCCTCCCGCCCCCCCGCCCAGCGACCACAAACC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAcc                                                                                                 <  2:431001/150‑1 (MQ=255)
                                                           ccAGCTTAGCGCGGGCACTCCACGGGGGAAAAAGTTTATTCCTCAGCGCAAATAAATTGACTCCCGCCCCCACCCCCAGCGCCCACAAAAC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                            <  2:311777/149‑1 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                       >  2:2195697/1‑150 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                       >  1:542782/1‑150 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                       >  2:428956/1‑150 (MQ=255)
                                                                 tatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCCAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCg                                                                                      >  1:4044535/1‑150 (MQ=255)
                                                                  atCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGc                                                                                     >  1:3511659/1‑150 (MQ=255)
                                                                    cgcgTGCACTACAACGGCGAAAAAGTTTATTCCCCAGCGCAAAGAAAATGACTCCCGCCACCCCGCCCAGCGACCAAAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt                                                                                   <  2:3674019/150‑1 (MQ=255)
                                                                     gcgTGCACTACAACGGCGAAAAAGTTTATTACCCAGCGCAAAGATAATGACTCCCGCCACCCCGCCCAGCGACCACAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtg                                                                                  <  2:2314278/150‑1 (MQ=255)
                                                                      cgTGCACTACAACGGCGAAAAATTTTATTACCCAGCGAAAAGATAAGGACTCCCGCCCCCCCGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                                 <  2:1496620/150‑1 (MQ=255)
                                                                         gCACTACAACGGCGGAAAAGTTTATTCCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcc                                                                              <  1:3711414/150‑1 (MQ=255)
                                                                         gCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcc                                                                              >  2:1446025/1‑150 (MQ=255)
                                                                          cACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccc                                                                             >  1:1012907/1‑150 (MQ=255)
                                                                          cACTACAACGGCGAAAAATTTTATTCCCCAGCGCAAAGATAATGCCTCCCGCCCCCCCGCCCAGCGACCACAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccc                                                                             <  2:2648354/150‑1 (MQ=255)
                                                                           aCTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGCCTTCCGCCACCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                            <  1:2701774/150‑1 (MQ=255)
                                                                           aCTACAACGGCGGAAAAGGTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                            >  1:1877028/1‑150 (MQ=255)
                                                                            ccacacCGGCGGAAAAGTTTATTCCTCGGCAAAAAGATAATCCCTCCCGCCCCCGCGCCGAGGGACCAAAATTC‑CCCAACCGCCGAGGCCGAGTAGCCGGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                           <  2:1329052/148‑1 (MQ=255)
                                                                            cTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                           >  2:1610060/1‑150 (MQ=255)
                                                                            cTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                           >  1:25995/1‑150 (MQ=255)
                                                                              acacCGGCGAAAAATTTTATTACCCAGCGCAAAGATAGTGTCTCCCGCCACCACGCCCAGCGACCAAAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTg                                                                         <  2:3269158/150‑1 (MQ=255)
                                                                              aCAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTg                                                                         <  1:3677107/150‑1 (MQ=255)
                                                                                 aCGGGGAAAAAGTTTTTTCCCCGGGGCAAAGATAATGCCTCCCGCCACCCCGCCCAGCCACCAAAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                                      <  2:2645759/150‑1 (MQ=255)
                                                                                    gcggAAAAGTTTATTACTCAGCGCAAAGATGATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                                   >  2:2381673/1‑150 (MQ=255)
                                                                                    gcgaAAAATTTTATCACCCAGCCCAAAGATAATGCCTCCCGCCACCACGCCCAGCGACCACAAACC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                                   <  2:1371504/150‑1 (MQ=255)
                                                                                     cggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                                  >  1:1482974/1‑150 (MQ=255)
                                                                                      ggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaac                                                                 >  1:3507867/1‑150 (MQ=255)
                                                                                        aaaaGTTTATTACTCAGCGAAAAGATAATGACTTCCGCCCCCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                               <  1:536494/150‑1 (MQ=255)
                                                                                          aaGGTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGCAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaac                                                             >  2:2621319/1‑150 (MQ=255)
                                                                                           gttttATTACTCAGCAAAAAGAAAATGCCTCCCCCCCCCCCGCCCAGCGACCACAAACC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                            <  2:2001286/148‑1 (MQ=255)
                                                                                           attttATTCCCCAGCAAAAAGAAAATGCCTCCCCCCCCCCCCCCCAGCGCCCAAAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                            <  2:163545/148‑1 (MQ=255)
                                                                                           attttATTCCCAAGCGCAAAGATAATGACTTCCGCCACCCCCCCCAGCGCCCCCAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                            <  1:801949/148‑1 (MQ=255)
                                                                                             tttATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                          >  2:581078/1‑150 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAg                                                         >  2:271882/1‑150 (MQ=255)
                                                                                                attaCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGc                                                       >  2:274319/1‑150 (MQ=255)
                                                                                                attaCCCAGGCCAAAAATAACGACTCCCGCACCCCCACCCAGC‑ACCAAAAACACCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCTGCATGTGCCCCCTGCAATAAACAAACGCAGGc                                                       <  2:1268390/150‑1 (MQ=255)
                                                                                                 ttccTCACCGCAAAGATAATGCCTCCCGCCACCACCCCCAGCGCCCAAAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGcc                                                      <  2:1654948/147‑1 (MQ=255)
                                                                                                 ttaCTCAGCGCAAAGATAATGACTCCCGCCACCGCGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGcc                                                      <  1:2125706/150‑1 (MQ=255)
                                                                                                     ccaccgaaaaGATAATGCCTCCCGCCCCCACGCCCAGCGACCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaa                                                  <  2:1708014/143‑1 (MQ=255)
                                                                                                        gcgcAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgc                                               >  1:277419/1‑150 (MQ=255)
                                                                                                        gcgaAAAGATAATGCCTCCCGCCACCCCGCCCAGCGCCCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgc                                               <  1:2863159/150‑1 (MQ=255)
                                                                                                           cAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGcc                                            >  1:1776209/1‑150 (MQ=255)
                                                                                                           cAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGcc                                            >  2:1868108/1‑150 (MQ=255)
                                                                                                           aaaaGATAATGCTTCCCGCCCCCGCCCCCGGCGACCAAAATCC‑CCCCCCCGCCGAGGCTGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGcc                                            <  2:1913458/149‑1 (MQ=255)
                                                                                                              aGAAAATGACTCCCGCCCCCACGCCCAGCACCCAAAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGt                                         <  2:3370528/150‑1 (MQ=255)
                                                                                                                aTAATGATTCCCGCCCCCACCCCCAGCGACCACAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                                       <  2:1276362/150‑1 (MQ=255)
                                                                                                                 tAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                      >  1:163414/1‑150 (MQ=255)
                                                                                                                 tAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                      >  2:3028277/1‑150 (MQ=255)
                                                                                                                  aaTGACTCCCGCCCCCCCGCCCAGCGCCCAAAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTg                                     <  2:1776209/150‑1 (MQ=255)
                                                                                                                       cTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGaa                                >  1:3946778/1‑150 (MQ=255)
                                                                                                                        ttCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAc                               >  1:1052744/1‑150 (MQ=255)
                                                                                                                             cccccacGCCCAGCGCCCAAAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcg                          <  2:59662/147‑1 (MQ=255)
                                                                                                                              caccacGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                         >  1:3380031/1‑150 (MQ=255)
                                                                                                                              caccacGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                         >  1:3405614/1‑150 (MQ=255)
                                                                                                                               accacGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGcc                        >  1:41754/1‑150 (MQ=255)
                                                                                                                                  ccGCCCAGCGCCCACAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAAc                     <  2:2814637/149‑1 (MQ=255)
                                                                                                                                    gCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa                   >  1:2258312/1‑150 (MQ=255)
                                                                                                                                     cccAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCAc                  >  2:4027694/1‑150 (MQ=255)
                                                                                                                                       cAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGc                >  1:2335733/1‑150 (MQ=255)
                                                                                                                                          cGACCACAAATC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGa             <  2:1603114/150‑1 (MQ=255)
                                                                                                                                          cGACCAAAAATC‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGa             <  1:3876157/150‑1 (MQ=255)
                                                                                                                                           gACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc            >  2:294119/1‑150 (MQ=255)
                                                                                                                                           gACCAAAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc            >  2:2391253/1‑150 (MQ=255)
                                                                                                                                             ccACAAACC‑CCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCa          <  2:683695/150‑1 (MQ=255)
                                                                                                                                                     a‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGc  >  2:1186988/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA‑CCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGC  >  AP012306/2212604‑2212902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑