Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306800,6750AC24.9% 548.5 / 32.2 233T192P (ACC→CCC) lolAchaperone for lipoproteins
Reads supporting (aligned to +/- strand):  ref base A (133/42);  new base C (0/58);  total (133/100)
Fisher's exact test for biased strand distribution p-value = 7.71e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 292 total aligned reads displayed.

GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAAC  >  AP012306/800526‑800823
                                                                                                                                                     |                                                                                                                                                    
gCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTACCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGACATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCa                                                                                                                                                      <  2:3355143/150‑1 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAcc                                                                                                                                                    >  1:3537346/1‑150 (MQ=255)
    tGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAcccc                                                                                                                                                  <  2:2833139/150‑1 (MQ=255)
     ggCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccg                                                                                                                                                 <  2:604200/150‑1 (MQ=255)
      gCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgc                                                                                                                                                >  1:3814093/1‑150 (MQ=255)
        aaTCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgccg                                                                                                                                              >  2:1403276/1‑150 (MQ=255)
        aaTCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgccg                                                                                                                                              >  1:1638440/1‑150 (MQ=255)
         aTCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCcgccgc                                                                                                                                             >  1:303662/1‑150 (MQ=255)
              aaGCATTTCACCATTACCGTGGGAGTTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCATTTATCAACTAAAACCCCAGAAAAATGGGGCTGTGGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGc                                                                                                                                        <  2:4341235/150‑1 (MQ=255)
               aGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCg                                                                                                                                       >  1:503754/1‑150 (MQ=255)
                 cAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTc                                                                                                                                     >  2:1494067/1‑150 (MQ=255)
                     tCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACgg                                                                                                                                 >  2:2147478/1‑150 (MQ=255)
                     tCACCATTAACGGGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACgg                                                                                                                                 <  2:3399807/150‑1 (MQ=255)
                        ccATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAg                                                                                                                              <  1:685737/150‑1 (MQ=255)
                          aTTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCTCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAgat                                                                                                                            >  1:1580198/1‑150 (MQ=255)
                          aTTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAgat                                                                                                                            >  2:2880763/1‑150 (MQ=255)
                           ttAACGTGGGACGTGATGGCACAACCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAAGGGGGTTGTGGATGCAGCGAATTTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAgatg                                                                                                                           <  2:4154266/150‑1 (MQ=255)
                               cGTGGGACTTGATGGCACAATCAATCATTTTAGCGCGGGGGAGCAGGACGACCAGGGCGCAATTTATCAACTAAAATCCCACCAAATGGGGGCTGTGGATGCAGCAAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCa                                                                                                                       <  2:46724/150‑1 (MQ=255)
                               cGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCa                                                                                                                       >  2:2667419/1‑150 (MQ=255)
                                 tGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAAc                                                                                                                     >  2:1103668/1‑150 (MQ=255)
                                     aCGTGATGGCACAATACATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTaa                                                                                                                 >  2:3770631/1‑150 (MQ=255)
                                      cGTGAGGGCACAATCCATTAGTTTGGCGGGGTGGAGCGGGACGATCAGCGCAGCAGTTATCAACAGAAATCCCAGAAAAATGGGGCTGGGGATGCGGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAg                                                                                                                <  2:3101344/150‑1 (MQ=255)
                                         gATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTag                                                                                                             >  1:61876/1‑150 (MQ=255)
                                         gATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTag                                                                                                             >  2:2260216/1‑150 (MQ=255)
                                           tGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTagag                                                                                                           >  2:3986238/1‑150 (MQ=255)
                                            ggCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAgg                                                                                                          <  1:2589357/150‑1 (MQ=255)
                                             gCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGc                                                                                                         >  1:820267/1‑150 (MQ=255)
                                                 aaTCCATCAGTTTAGGGCGGTGGAGCAGAACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGAAAAATGGGGCTGGGGATGCAGCAAAATTTCCCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCt                                                                                                     <  2:527879/150‑1 (MQ=255)
                                                 aaTCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCt                                                                                                     >  2:3071658/1‑150 (MQ=255)
                                                      aTCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtg                                                                                                <  1:3322519/150‑1 (MQ=255)
                                                       tCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAAGGGGGCTGTGGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtga                                                                                               <  2:2478881/150‑1 (MQ=255)
                                                           tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                           >  1:1010709/1‑150 (MQ=255)
                                                           tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                           >  2:2335495/1‑150 (MQ=255)
                                                           tttAGCGCGGGGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCaa                                                                                           <  2:3715537/150‑1 (MQ=255)
                                                            ttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAAt                                                                                          <  1:3907827/150‑1 (MQ=255)
                                                             tAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATc                                                                                         >  1:2416425/1‑150 (MQ=255)
                                                             tAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTAAAACCCCAGAAAAAGGGGGCTGGGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATc                                                                                         <  2:1193799/150‑1 (MQ=255)
                                                                  gggTGGAGCAGGACGATCAGCGCAGCAGTTACAAACTGAAATCCCAGCAAAATGGGCTTGCGGAGGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCg                                                                                    <  2:78525/149‑1 (MQ=255)
                                                                   ggTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGc                                                                                   >  1:1548621/1‑150 (MQ=255)
                                                                    gTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt                                                                                  <  1:783411/150‑1 (MQ=255)
                                                                    gTGGAGCAGGACGATCAGCGCAGCAGTTATAAACTGAAATCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt                                                                                  <  1:138691/150‑1 (MQ=255)
                                                                       gagaAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGa                                                                               <  2:3506431/150‑1 (MQ=255)
                                                                       gAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGa                                                                               <  1:2152287/150‑1 (MQ=255)
                                                                        aGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAt                                                                              >  2:2935943/1‑150 (MQ=255)
                                                                             gACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACTCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTc                                                                         >  2:317678/1‑150 (MQ=255)
                                                                              aCGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCg                                                                        <  2:3297562/150‑1 (MQ=255)
                                                                               cGACCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAATGGGGCTGGGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCgg                                                                       <  2:2302030/150‑1 (MQ=255)
                                                                                  tCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGata                                                                    <  2:3918208/150‑1 (MQ=255)
                                                                                   cAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataa                                                                   >  1:3432852/1‑150 (MQ=255)
                                                                                   cAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataa                                                                   <  1:3019674/150‑1 (MQ=255)
                                                                                    aGCGCAGCAGTTATCAACTAAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAATTTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                  <  1:2018247/150‑1 (MQ=255)
                                                                                     gcgcAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataata                                                                 <  1:2962591/150‑1 (MQ=255)
                                                                                      cgcAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATAc                                                                >  2:1329985/1‑150 (MQ=255)
                                                                                        cagcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtt                                                              >  2:1539997/1‑150 (MQ=255)
                                                                                           cagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTc                                                           >  2:1875005/1‑150 (MQ=255)
                                                                                            agTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCa                                                          <  2:1986228/150‑1 (MQ=255)
                                                                                             gTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCaa                                                         <  1:183752/150‑1 (MQ=255)
                                                                                             gTTATCAACTAAAACCCCAGAAAAATGGGGCTGTGGATGCAGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCaa                                                         <  2:3548533/150‑1 (MQ=255)
                                                                                              ttATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAc                                                        >  2:3235491/1‑150 (MQ=255)
                                                                                               tATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAcc                                                       <  1:581061/150‑1 (MQ=255)
                                                                                                aTCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACct                                                      >  2:3961213/1‑150 (MQ=255)
                                                                                                 tCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCAAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctc                                                     <  1:1763442/150‑1 (MQ=255)
                                                                                                 ccAACTGAAACCCCAGAAAAATGGGGTTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctc                                                     <  2:2599859/149‑1 (MQ=255)
                                                                                                  cagctaaaaTCCCAGCAAAAGGGGGCTGGGGGTGGAAACAAATTTCCTTACACACCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctct                                                    <  2:4051492/144‑1 (MQ=255)
                                                                                                      tGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGcc                                                >  2:3737840/1‑150 (MQ=255)
                                                                                                       aaaaCCCCAGCAAAATGGGGCTGTGGATGCAGCAAATTTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcg                                               <  2:971334/149‑1 (MQ=255)
                                                                                                         aaccccAGAAAAGTGGGGCTGTGGATGCAGAGAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcgcg                                             <  2:203336/147‑1 (MQ=255)
                                                                                                          aTCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcgcgc                                            >  2:1893951/1‑150 (MQ=255)
                                                                                                          aTCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcgcgc                                            >  1:482429/1‑150 (MQ=255)
                                                                                                             ccAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTGTCGGATAATACTTTTCAACCTCT‑GCCGCGCGTAt                                        <  2:3861865/150‑1 (MQ=255)
                                                                                                              cAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTAt                                        >  1:1740785/1‑150 (MQ=255)
                                                                                                               aGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATg                                       >  1:2294605/1‑150 (MQ=255)
                                                                                                                gCAAAGTGGGGTTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGc                                      <  1:959873/150‑1 (MQ=255)
                                                                                                                   aaaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGc                                   <  1:1083189/150‑1 (MQ=255)
                                                                                                                   aaaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGc                                   >  2:882607/1‑150 (MQ=255)
                                                                                                                    aaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGcc                                  >  2:1134078/1‑150 (MQ=255)
                                                                                                                    aaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGcc                                  >  2:25344/1‑150 (MQ=255)
                                                                                                                      tGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAg                                >  1:2565164/1‑150 (MQ=255)
                                                                                                                       ggggCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGa                               <  1:1791484/150‑1 (MQ=255)
                                                                                                                       ggggCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGa                               <  1:28261/150‑1 (MQ=255)
                                                                                                                        gggCTGTGGATGCAGCGAAATTTACCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaa                              <  1:3986439/150‑1 (MQ=255)
                                                                                                                            tgtgGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAtt                          >  2:3439373/1‑150 (MQ=255)
                                                                                                                                 aTGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGca                     <  1:3384887/150‑1 (MQ=255)
                                                                                                                                 aTGCAGCAAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGca                     <  1:1124607/150‑1 (MQ=255)
                                                                                                                                  tGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGcac                    >  2:3428207/1‑150 (MQ=255)
                                                                                                                                    caacGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAg                  >  1:2508660/4‑150 (MQ=255)
                                                                                                                                    cAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAg                  <  1:4189908/150‑1 (MQ=255)
                                                                                                                                    cAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAg                  >  2:1812149/1‑150 (MQ=255)
                                                                                                                                      gCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGta                <  1:4142008/150‑1 (MQ=255)
                                                                                                                                      gCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGta                <  1:3709133/150‑1 (MQ=255)
                                                                                                                                      gCGAAATTTACCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGta                <  1:3670618/150‑1 (MQ=255)
                                                                                                                                        gAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtata              >  2:1261017/1‑150 (MQ=255)
                                                                                                                                          aaTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATc            >  1:2184590/1‑150 (MQ=255)
                                                                                                                                           aTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCg           >  2:1010905/1‑150 (MQ=255)
                                                                                                                                           aTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCg           >  2:2210492/1‑150 (MQ=255)
                                                                                                                                              tcctttCACCCCGCCTCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCATAGTATATCGGcc        <  2:4282305/146‑1 (MQ=255)
                                                                                                                                               aCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCa       <  1:2669559/150‑1 (MQ=255)
                                                                                                                                                ccTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAg      <  2:57314/150‑1 (MQ=255)
                                                                                                                                                ccTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAg      <  1:94373/150‑1 (MQ=255)
                                                                                                                                                 cTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc     >  2:161639/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAAC  >  AP012306/800526‑800823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑