Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC24.4% 349.8 / 19.5 135L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (88/14);  new base C (0/33);  total (88/47)
Fisher's exact test for biased strand distribution p-value = 1.06e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 217 total aligned reads displayed.

ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCTATG  >  AP012306/3182712‑3183009
                                                                                                                                                      |                                                                                                                                                        
      cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGAGTTCCCCTTGCGTGGTGTTTGGCGCAACGCTGTTGGGTACCTGGTGGTGGGGGGCCAGTC‑GCCTTAATTTTccccac                                                                                                                                                    >  2:3682685/1‑148 (MQ=255)
      cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCATGATGATGGGCGCAACCCTGTTGCTTTCCTTGGGGGGGCGGGCCCGTC‑GCCCTTATTTTcccccc                                                                                                                                                    >  2:1381596/1‑148 (MQ=255)
        cccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTGCCCCTGGCGTGTTGATTGGCGCAACGCTGGTGCTTTCCTGGGGGTGGGAGGCCCGGCGGCCTTATTTTTCcccccc                                                                                                                                                   >  2:713856/1‑150 (MQ=255)
              cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTTGGATTGTGATGGGCGGAACGCTGATTCTTTCCTGGTGGTGGGAGGGCAGGC‑GCCTTATTTTTTCCCCCCAGCcaaa                                                                                                                                            >  2:1466344/1‑150 (MQ=255)
                 tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCACCGCTGATGCTTACCTGGGGGTGGCAGGCCAGTC‑CCCTTAATTTCCCCCGCCAGCAAAAAGc                                                                                                                                         <  2:2194586/150‑1 (MQ=255)
                  ccGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTTTTTATGGCGGGCATTGCCCCTGGAATGATGATGGGCGCACCGCTGATGCTTACCTGGGGGTGGCAGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCa                                                                                                                                        <  2:2459793/150‑1 (MQ=255)
                  ccGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCCTGATTATGGGCGCAACACCGATGCTTTCCTGGGGGTGGGGGGCCCGAC‑GCCGTCATTTACCCCCCCCGCAAAAAGCa                                                                                                                                        >  2:2407452/1‑150 (MQ=255)
                  ccGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCGGGCCAGTC‑GCCTTTATTTTTCCCGCCAGCCAAAAACa                                                                                                                                        >  2:1802934/1‑150 (MQ=255)
                  ccGTTTATGATCTTCGCCGTTTCAGGCGAATTATCTCTCAGTAAGTTTTTTTTGGGGGCCATCCCCACGGACCTGGTAAGGGGGGAACGGCTGCTCGTTACCGGGGGGTGGGAGGCCAGCC‑GCATTAATTTACCCCGCCAGCAAAAAGCa                                                                                                                                        <  2:4156930/150‑1 (MQ=255)
                   cGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTTCCCCGGCATGATGATGGGGGCAAAGCTGATGCTTTTCTGGTGGGGGGAGGGCAGTC‑GCCTTTATTTTTCCCCCCAGCAAAAAACaa                                                                                                                                       >  2:1505946/1‑150 (MQ=255)
                   cGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCaa                                                                                                                                       <  1:1521355/150‑1 (MQ=255)
                    gTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTTCCCTGGCCTGGTGATGGGCGGCACGCTGATGCTTACCCCGGGGGGGGGGGGCAGTT‑GCCCTTATTTTACCCGCCCGCcaaaaagaac                                                                                                                                      >  2:3961273/1‑145 (MQ=255)
                     tttATTATCTTCGGCGTTTCCAGCGGATTATCAATAAGTAAGCTGTTTAGGGGGGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTACCGGGTGGTGGCGGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACg                                                                                                                                     <  2:1144584/150‑1 (MQ=255)
                      ttattaTCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCGTTTCCCCTGGCCTTGTGATGGGTGCAAAGCGTCTTCTTTCCCGGTGGTGGGGGGCCCGGC‑GCCTTTATTTTTCCCCCCCGCCAAAaacaacaa                                                                                                                                    >  2:4176621/1‑148 (MQ=255)
                       tattatCTTCGGCGTTTCCGGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTCCCTGGGGGTGGCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACGAt                                                                                                                                   <  2:3334710/150‑1 (MQ=255)
                         ttatCTCCGGCTTTTCCAGCGGATTATCCATCAGAAGGTTTTTTATGGCGGGCTTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCGGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGc                                                                                                                                 <  2:2538914/150‑1 (MQ=255)
                           ttCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGTTGTTTAGGGCGGGCATTGCCCTTGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGGGGGGGCAGGCCAGTC‑CCCTAATTTTACCCCGCCAGCAAAAAGCAACGATGCAg                                                                                                                               <  2:799251/149‑1 (MQ=255)
                            tCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAgg                                                                                                                              <  2:445701/150‑1 (MQ=255)
                             cTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCATTCCCCCGGCAATGTGATGGGGGCCACGCTGTATCTTTACTCGGGGGGGGAGGCCCGGC‑GCCGTTATTTTCCCCCCCCGCGAAAAAAAACCATGCCGGa                                                                                                                             >  2:2273017/1‑150 (MQ=255)
                               tcgtggTTTCCAGGGGATTATCCACAAGTAAGCTGTTGATGGCGGGCATGGCCCCGGGAATGATGAGGGGAGCAACGCGGATGCTTACCTGGTGGGGGCGGGCCGGTC‑GCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGaaa                                                                                                                           <  2:525477/145‑1 (MQ=255)
                                cGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCGTTGTCCCCCGCGTGGTGGTGGGGGGAACGCTGATGGTTACCTGGGGGGGGGGGGGCCGGC‑CCCCTTTTTTTCCCCGCCCCCCAAAAACAAAGAAGCAGGaaaa                                                                                                                          >  2:3394005/1‑149 (MQ=255)
                                 ggCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTTTCCCCGGCCTGATGATGGGGGCAAAGCTTGTGCTTTCCTGGGGGGGGGCGGGCAGGC‑GCCCTTATTTACCCCCCCCGCCAAAAACAACGATGCAGGgaaac                                                                                                                         >  2:172786/1‑148 (MQ=255)
                                   ctttcccAGCGAATTACCCACCAGTAACCTGTTTATGGCGGGCATTGCCCCGGGCATGATGAGGGGCGCAACGCTGATGCTTACCTGGGGGGGGCGGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTg                                                                                                                       <  2:3072243/145‑1 (MQ=255)
                                      ttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCATGATGATGGGGGCAACGCTGATGCTTACCTGGTGGGGGGAGGCCCGTC‑GCCTTTATTTTCCCCCCCCGCAAAAAACAAACATGCAGGAAATCTGGCa                                                                                                                    >  2:3419760/1‑150 (MQ=255)
                                      ttCCAGCGGATTACCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCGGGTGGGGGCAGGCCAGTC‑GCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCa                                                                                                                    <  1:2963242/150‑1 (MQ=255)
                                       tCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCATGATGATGGGGGCAACGCTGATGCTTACCCGGGGGTGGCAGGCCCGTC‑GCCCTTATTTTTCCCCCCCCCAAAAAACAACGAAGCAGGAAAACTGGCAc                                                                                                                   >  2:1291828/1‑150 (MQ=255)
                                              gATTACCCATCAGTAAGCTGTTTAGGGCGGGCATTCCCCCGGGCATGATGAGGGGCGCACCGCTGAGGCTTCCCTGGGGGGGGCGGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTg                                                                                                            <  2:2236011/150‑1 (MQ=255)
                                               aTTATCCATCAGTAAGCTGTTTATGGCGGGCATTTGCCCCGGCATGATTATTGGGGCCACGCTGATGCTTTACTTGGGGGGGGGGGCCAGGC‑GCCTTAATTTTTCCCCCCCCCAAAAAAAAAAAAAGCAGGAAAACTTGCACCCtttttt                                                                                                           >  2:839476/1‑148 (MQ=255)
                                                      aTCAGTAAGCTGTTTATGGCGGGCATTTTCCCCCGGATGGTGATGGGGGGGAACCTCATGCTTTCCTGGTGGTGGCAGGGCCGTC‑GCCCTTATTTTCCCCGCCCGCCAAAAGCAAAGATGCAGGGAAACTGGCACTCTTTTTTTTcccgg                                                                                                    >  2:4252107/1‑147 (MQ=255)
                                                       gcAGTAAGCTTTTTATGGCGGGTATTGCCCCTGGCATGAGGTGGGGCGCAACGCTGATGCTTACCTGGGGGGGGCAGGCCAGTC‑GCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTa                                                                                                   <  2:665484/149‑1 (MQ=255)
                                                          gaaaGCTTTTTATGGGGGGCATGCCCCGCGGCATGATGATGGGCGCAACGCTCTTGCTTACCTGGTGGTGGCAGCCCAGCC‑CCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCt                                                                                                <  2:892303/148‑1 (MQ=255)
                                                          gTAAGCTGTTTATGGCGGGCATTGTCCCCGGCCTGGTGGTGGGGGCAACGCTGGTGGTTACCTGGTGGGGGCAGGGCCGGC‑GCCTTAATTTTACCCGCCCGCCAAAAAAAACGAAGCAGGAAATCTGGGACTCTTTTTTCTCCGGTATCt                                                                                                >  2:3592894/1‑150 (MQ=255)
                                                              gCTGTTTATGGCGGGCATTTCCCCCGGCATGATGATGGGGGCAACGCTGATGCTTTCCTTGTGGGGGCAGGCCAGTC‑GCCTTTATTTACCCCCCCAGCCAAAAACAACGATGCAGGAAAACTGGGGCCCTTTTTTTTCCGGTATCTtggg                                                                                            >  2:1882183/1‑149 (MQ=255)
                                                                tGTTTATGGCGGGCATTGCCCCCGGCATGGTGATGGGGGCAACGCTGATGGTTAACTGGGGGGGGCAGGCCAGTC‑GCCCTGATTTAACCCGCCAGCAAAAAGCAACGAAGCAGGGAAACTGGGACTCTTTTGTCTCCGGGATCTGGgcgc                                                                                          >  2:347414/1‑150 (MQ=255)
                                                                 gTTTAGGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCCGGGTGGTGGCAGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                                         <  2:2435258/150‑1 (MQ=255)
                                                                        gCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                                  >  2:548482/1‑150 (MQ=255)
                                                                        gCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                                  >  1:928008/1‑150 (MQ=255)
                                                                        gCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCGGGTGGTGGCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                                  <  1:97364/150‑1 (MQ=255)
                                                                         cGGGCATTGCCCCTGGCATGATGATGGGCGCACCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                 <  1:1739340/150‑1 (MQ=255)
                                                                         cGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                 >  1:1026908/1‑150 (MQ=255)
                                                                         cGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                 >  2:1697732/1‑150 (MQ=255)
                                                                         cGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                 >  1:3679430/1‑150 (MQ=255)
                                                                          gggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                                >  1:1061914/1‑150 (MQ=255)
                                                                          gggCATTCCCCCGGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                                <  2:1120802/150‑1 (MQ=255)
                                                                          ggccATCGCCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                                <  2:2749379/147‑1 (MQ=255)
                                                                            gCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAg                                                                              >  1:3743653/1‑150 (MQ=255)
                                                                              aTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                            >  1:2921785/1‑150 (MQ=255)
                                                                                tGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcat                                                                          >  1:2287391/1‑150 (MQ=255)
                                                                                 gCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                         >  1:4301402/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                       >  2:345927/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                       >  1:3191239/1‑150 (MQ=255)
                                                                                     cTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatta                                                                     <  1:325308/150‑1 (MQ=255)
                                                                                      tGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                    >  2:1376832/1‑150 (MQ=255)
                                                                                       ggCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                   <  1:740876/150‑1 (MQ=255)
                                                                                          atgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGc                                                                >  1:3819048/1‑150 (MQ=255)
                                                                                           tgatgatgGGCGCAAGGCTGATGCTTACCTGGTGGTGGCGGGCCAGCC‑GCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑g                                                              <  2:3909674/150‑1 (MQ=255)
                                                                                           tgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑g                                                              <  1:4194770/150‑1 (MQ=255)
                                                                                            gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑gg                                                             >  1:3875065/1‑150 (MQ=255)
                                                                                              tgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑gggg                                                           >  2:2110575/1‑149 (MQ=255)
                                                                                                atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGgtt                                                         >  2:3753095/1‑150 (MQ=255)
                                                                                                 tgGGCGCAACGCTGATGCTTACCTGGGGGTGGCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTc                                                        <  2:1070621/150‑1 (MQ=255)
                                                                                                 ggggCGCACCGCTGATGCTTACGTGGTGGGGGCGGGCGAGTC‑CCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTc                                                        <  2:3140161/149‑1 (MQ=255)
                                                                                                  ggccgcACCGCTGATCCTTACCTGGGGGGGGCAGCCCAGTC‑GCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCg                                                       <  1:2448999/147‑1 (MQ=255)
                                                                                                     cgcAACGCTGGTTCTTTCTTGGGGGTGGCGGGCCAGTC‑GCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑c                                                  <  2:3657611/150‑1 (MQ=255)
                                                                                                       cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGCTTGG‑GT‑cct                                                >  2:4299474/1‑149 (MQ=255)
                                                                                                        aaCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTGTTG‑CT‑CCgg                                               >  2:1182432/1‑150 (MQ=255)
                                                                                                             tgtggCTTCCCTGGGGGTGGCTTCCCATCC‑CCTTTAATTGCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGt                                          <  2:4221530/146‑1 (MQ=255)
                                                                                                             tGATGCTTCCCTGGGGGTGGCAGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGt                                          <  2:1324717/150‑1 (MQ=255)
                                                                                                               aTGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTTATCATTATTGGC‑GGGGTTTG‑CT‑CCCGCCTGttt                                        >  1:1946534/1‑150 (MQ=255)
                                                                                                                tGCTTCCCGGGTGGTGGCGGGCCGGTC‑CCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTa                                       <  2:3105049/150‑1 (MQ=255)
                                                                                                                tGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCGGGCTGTTTa                                       >  1:2727501/1‑150 (MQ=255)
                                                                                                                 gCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCCGCCTGtttta                                      >  1:1992619/1‑148 (MQ=255)
                                                                                                                    tACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGGGCA‑CT‑CCCGCCCGTTTTcccc                                   >  2:857734/1‑150 (MQ=255)
                                                                                                                       gtggtgggggCCGGCACGTC‑GCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑c                               <  2:3276331/149‑1 (MQ=255)
                                                                                                                          gtggtggCAGGCCAGTC‑GCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGa                            <  1:1594807/150‑1 (MQ=255)
                                                                                                                            ggtggCAGGCCAGTC‑GCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAg                          <  1:1145846/150‑1 (MQ=255)
                                                                                                                               ggCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCCGCCTGTTTTTCCCTA‑ccccaaccg                       >  2:1260227/1‑143 (MQ=255)
                                                                                                                                 cAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCggg                     <  1:2460724/150‑1 (MQ=255)
                                                                                                                                 cAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CTCCCGGCCGTTTTTCCCCAC‑CCCAACCCgg                      >  2:1967601/1‑150 (MQ=255)
                                                                                                                                 cAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCCGCCCGTTTACCCCTA‑CCCAAACCggg                     >  1:1798446/1‑150 (MQ=255)
                                                                                                                                  aGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTT‑CTCCCGGCCTTTTTTCCCCCT‑CCGAAACCggg                     >  1:309529/1‑150 (MQ=255)
                                                                                                                                     ccggTC‑GCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCg                 <  2:670837/147‑1 (MQ=255)
                                                                                                                                     ccAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTGTTG‑CT‑CCCGCCTGTTTTTCCCTC‑CCCCAGCCcgggggc                 >  2:755312/1‑147 (MQ=255)
                                                                                                                                      cAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTGTCG‑CT‑CCCGCCTGTTTTTCCCTC‑CCCAAGCCGGGGGCgg                >  2:167122/1‑149 (MQ=255)
                                                                                                                                      cAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCCGCCTGTTTTTCCCTA‑CCCAAGCCGGGGGCGt                >  2:2466137/1‑150 (MQ=255)
                                                                                                                                       aGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTTT‑CT‑CCGGCCCGTTTTTCCCCA‑CCCAAGCCCGGGcccgt               >  2:2681921/1‑147 (MQ=255)
                                                                                                                                       aGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTT‑CT‑CCTGCCCGTTTTCCCCCC‑CCCAACCCCGGGCCGtt               >  2:3793169/1‑150 (MQ=255)
                                                                                                                                        ggcccccTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGtt               <  1:3653040/145‑1 (MQ=255)
                                                                                                                                         tC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTT‑CT‑CCGGCCTGTTTATCCCTA‑CCCACACCCGGGCCGTtgc             >  1:1299464/1‑150 (MQ=255)
                                                                                                                                          tcgttttAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTtgc             <  2:632368/145‑1 (MQ=255)
                                                                                                                                          c‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCCGCCCGTTTTCCCCTA‑CCGAAGCCCGGGGCGGtgct            >  1:538651/1‑150 (MQ=255)
                                                                                                                                            gctttAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTtgctg           <  2:2777125/147‑1 (MQ=255)
                                                                                                                                            gCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTtgctg           <  1:2497022/150‑1 (MQ=255)
                                                                                                                                             ccTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTG‑TT‑CCGCCTTTTTTACCCTTA‑CAGAAGCCGGGGCCgcgctgg           >  2:2082894/1‑144 (MQ=255)
                                                                                                                                             ccTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCGGGtgctgc          >  1:3007556/1‑150 (MQ=255)
                                                                                                                                                tAATTTACCCCGCCAGCAAAAAGCAACAATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTTGTCC‑CCGGCTCTTTTTCCCCT‑‑CCCAAGCCCGGGCCGCTGCTGCttt       >  1:2202207/1‑150 (MQ=255)
                                                                                                                                                  aTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCt     <  1:1385047/150‑1 (MQ=255)
                                                                                                                                                  aTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCt     <  1:3519943/150‑1 (MQ=255)
                                                                                                                                                    ttACCCCGCCAGCAAAAAGCAGCGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGGTTTG‑CT‑CCCGCCCCTTTTCCCCCACCCAAACCCGGGGCCGTTG‑TGCTTTCttt   >  1:2031644/1‑148 (MQ=255)
                                                                                                                                                     tACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCTATg  <  1:761025/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTC‑GCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGC‑GGGTTTCG‑CT‑CCGGCCTGTTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCTATG  >  AP012306/3182712‑3183009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑