Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT22.5% 617.5 / 112.5 258S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (19/181);  new base T (58/0);  total (77/181)
Fisher's exact test for biased strand distribution p-value = 1.65e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 306 total aligned reads displayed.

CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938739‑3939036
                                                                                                                                                     |                                                                                                                                                      
cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttct                                                                                                                                                       >  1:770842/1‑150 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGTCCTtttttttt                                                                                                                                                      >  2:890569/1‑150 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCAtttttttttt                                                                                                                                                     >  1:3914814/1‑150 (MQ=255)
     aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttctttttc                                                                                                                                                  <  2:2053113/150‑1 (MQ=255)
       aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCAttttttttttttttt                                                                                                                                                >  1:537708/1‑147 (MQ=255)
       aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTg                                                                                                                                                <  1:2696480/150‑1 (MQ=255)
         aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGcc                                                                                                                                              <  2:1161459/150‑1 (MQ=255)
          aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCa                                                                                                                                             <  2:3288006/150‑1 (MQ=255)
          aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCa                                                                                                                                             <  1:95248/150‑1 (MQ=255)
           aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCAt                                                                                                                                            <  1:1304208/150‑1 (MQ=255)
             gCGTCATGAGTAGTACCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                          <  2:3596647/150‑1 (MQ=255)
               gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                        <  2:2532880/150‑1 (MQ=255)
                tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                                                                       <  1:2444794/150‑1 (MQ=255)
                 cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTCTTTTTTTTCCACTAGAAg                                                                                                                                      >  1:150085/1‑150 (MQ=255)
                 cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCACTTTTTTTTTTTTTTCCATTAGaaa                                                                                                                                      >  1:1434642/1‑149 (MQ=255)
                  aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                                     <  1:1147856/150‑1 (MQ=255)
                   tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTg                                                                                                                                    <  1:1225431/150‑1 (MQ=255)
                    gAGTAGTAACGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCGCCATACCTATACCAGTCAGAATTCATTTCAGGATGCCAGGTCGCGGATCTCCCTTGCGGC‑GGTCATTTTTTCTTTATCTGCCATGAGAAGTGa                                                                                                                                   <  2:3043571/150‑1 (MQ=255)
                      gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTTCCATGAGAAGGGAgc                                                                                                                                 >  1:677919/1‑150 (MQ=255)
                      gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                 <  1:3479726/150‑1 (MQ=255)
                       tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTTAGAAGTGAggg                                                                                                                                >  1:2817149/1‑148 (MQ=255)
                           aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                            <  1:3369304/150‑1 (MQ=255)
                           aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGCCTTTTTTTTTTTTTTTGCCTTTGGGAGTGAGAGCaga                                                                                                                            >  2:236892/1‑150 (MQ=255)
                            tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                           <  1:2905807/150‑1 (MQ=255)
                            tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                           <  2:3470147/150‑1 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAgg                                                                                                                        <  2:1380449/150‑1 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAgg                                                                                                                        <  1:3305208/150‑1 (MQ=255)
                                tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGAAATGGGCGCAGAGAggg                                                                                                                       >  1:1896797/1‑150 (MQ=255)
                                 aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                                                                                      <  1:3663757/150‑1 (MQ=255)
                                   tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAtt                                                                                                                    <  1:2418681/150‑1 (MQ=255)
                                    tggagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                   <  1:3227317/148‑1 (MQ=255)
                                    gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCGTTTTTTCTTTTTTTGTCATGAGAAGTGAGCGCCGAGCGGGAGTa                                                                                                                   >  2:2997962/1‑150 (MQ=255)
                                    gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                   <  1:895421/150‑1 (MQ=255)
                                    gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGGATTTTTTTTTTTTTTTTCAGGAGAAGGGAGGGGGGGGAGGGATTa                                                                                                                   >  2:1704036/1‑150 (MQ=255)
                                     agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                  <  1:788449/150‑1 (MQ=255)
                                     agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                  <  1:2724234/150‑1 (MQ=255)
                                        gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTTAGAAGTGAGCGCAGAGAGGGGTTATCtt                                                                                                               >  2:4019383/1‑150 (MQ=255)
                                        gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                               <  2:430273/150‑1 (MQ=255)
                                         tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                              <  2:121431/150‑1 (MQ=255)
                                           tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGcc                                                                                                            <  1:1784984/150‑1 (MQ=255)
                                            aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCGTTTTTTCTTTTTTTGGCACGTGGAGTGGGTGCCGTGAGGGATTATcaattct                                                                                                           >  2:2915268/1‑144 (MQ=255)
                                            aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                                           <  1:4140840/150‑1 (MQ=255)
                                             ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGAGAGGGGGTATTTTGGctg                                                                                                          >  2:1555430/1‑150 (MQ=255)
                                               cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgac                                                                                                        <  2:2126313/150‑1 (MQ=255)
                                                aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                       <  1:4257546/150‑1 (MQ=255)
                                                   aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGaa                                                                                                    <  2:1465946/150‑1 (MQ=255)
                                                    aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                                   <  2:498481/150‑1 (MQ=255)
                                                    aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                                   <  1:3932477/150‑1 (MQ=255)
                                                    aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTTTTTTTTTGCCATGAGAAGTGGGCGCAGGGAGGGGGTATCTTGCCTGGCTGAAc                                                                                                   >  2:2619103/1‑150 (MQ=255)
                                                     gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                                                                                  <  1:3834958/150‑1 (MQ=255)
                                                     gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                                                                                  <  1:2316801/150‑1 (MQ=255)
                                                       ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTGTCATGAGAAGAGAGCGCAGAGAGGGGATATCTTGTCTGACTGAAAT‑gg                                                                                               >  2:341631/1‑150 (MQ=255)
                                                       ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑gg                                                                                               <  2:3612009/150‑1 (MQ=255)
                                                           gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGAt                                                                                           <  1:3069278/150‑1 (MQ=255)
                                                             gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTGATTTTTTTTTTTTTTTCCCTCAGAAAAGAGGGCGGGGGGGGGGTATTTTGCCTTACTTAACA‑GGCGGTTa                                                                                         >  2:2463812/1‑150 (MQ=255)
                                                              tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTTAGAAGTGAGGGCAGGGAGGGGGTATTTTGCCTGACTTAACA‑GGCgggtta                                                                                        >  2:682244/1‑145 (MQ=255)
                                                              tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTaa                                                                                        <  1:2140113/150‑1 (MQ=255)
                                                               aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAg                                                                                       <  2:3770336/150‑1 (MQ=255)
                                                                  ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGGACT‑GGCGGTTAAGttt                                                                                    >  2:1486054/1‑150 (MQ=255)
                                                                  ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGttt                                                                                    <  1:1726471/150‑1 (MQ=255)
                                                                  ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGttt                                                                                    <  2:3535005/150‑1 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTc                                                                                   <  2:2299705/150‑1 (MQ=255)
                                                                     tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCcg                                                                                 <  1:827737/150‑1 (MQ=255)
                                                                      ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCcgc                                                                                <  1:3983593/150‑1 (MQ=255)
                                                                        aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACT‑GGCGATTTAGTTTCCCCGt                                                                              >  2:4071494/1‑150 (MQ=255)
                                                                         aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTa                                                                             <  1:378889/150‑1 (MQ=255)
                                                                          gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAc                                                                            <  2:4232733/150‑1 (MQ=255)
                                                                             ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCg                                                                         <  2:2163806/150‑1 (MQ=255)
                                                                                   cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                   <  1:4221920/150‑1 (MQ=255)
                                                                                        ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                              <  2:3157653/150‑1 (MQ=255)
                                                                                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                            <  2:386640/150‑1 (MQ=255)
                                                                                             cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                                         <  1:3284102/150‑1 (MQ=255)
                                                                                             cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                                         <  1:2964944/150‑1 (MQ=255)
                                                                                               ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGGTATTTTTCCTGACTGAACT‑GGCGATTAAATTTTCCCGGACCCGGCGTTTTGTttttttgga                                                       >  2:2337002/1‑145 (MQ=255)
                                                                                                gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                      <  1:1346049/150‑1 (MQ=255)
                                                                                                gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                      <  1:4029109/150‑1 (MQ=255)
                                                                                                   tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                                   <  1:999630/150‑1 (MQ=255)
                                                                                                       ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGata                                               <  1:2001215/150‑1 (MQ=255)
                                                                                                          aGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                            <  2:1934092/150‑1 (MQ=255)
                                                                                                                  aTGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGTGAAGGGGGGGGAGGGGGGGGGTATTTTTCCTGGCTGAACTGGGGGGTTAAGTTT‑CGCGGACCCCGCGGTTTGGTATTTTGGGTGGTGGTATTTaaaatccg                                    >  2:1994648/1‑144 (MQ=255)
                                                                                                                    gTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTTCCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                  <  1:1672753/150‑1 (MQ=255)
                                                                                                                    gTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                  <  2:3202768/150‑1 (MQ=255)
                                                                                                                    gTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                  <  1:1570665/150‑1 (MQ=255)
                                                                                                                     tCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTg                                 <  1:221999/150‑1 (MQ=255)
                                                                                                                      cgcgGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                                <  2:3827430/150‑1 (MQ=255)
                                                                                                                       gcgGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                               <  1:1032546/150‑1 (MQ=255)
                                                                                                                         ggATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                             <  1:2662912/150‑1 (MQ=255)
                                                                                                                             ctcGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                         <  2:2703972/150‑1 (MQ=255)
                                                                                                                               cGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                       <  2:2426725/150‑1 (MQ=255)
                                                                                                                                gTTTGCGGC‑GGTCATTTTTTC‑TTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg                     <  1:47079/150‑1 (MQ=255)
                                                                                                                                   tGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTc                   <  1:613240/150‑1 (MQ=255)
                                                                                                                                           gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa            >  2:31008/1‑150 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         >  2:1114310/1‑150 (MQ=255)
                                                                                                                                                tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt       >  2:1667724/1‑150 (MQ=255)
                                                                                                                                                tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt       >  2:3965/1‑150 (MQ=255)
                                                                                                                                                tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt       <  2:1152158/150‑1 (MQ=255)
                                                                                                                                                  tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg     <  2:826375/150‑1 (MQ=255)
                                                                                                                                                    tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   >  2:1877941/1‑150 (MQ=255)
                                                                                                                                                    tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   <  1:2048597/150‑1 (MQ=255)
                                                                                                                                                     ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg  >  2:2870972/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938739‑3939036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑